Staphylococcus phage SA75

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; Staphylococcus virus SA75

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0RSD3|A0A6C0RSD3_9CAUD Transcriptional activator OS=Staphylococcus phage SA75 OX=2681195 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.56VEE20 pKa = 3.88YY21 pKa = 11.22KK22 pKa = 10.82HH23 pKa = 6.75FGDD26 pKa = 4.47VDD28 pKa = 3.64NEE30 pKa = 3.66KK31 pKa = 10.93DD32 pKa = 3.32ALADD36 pKa = 3.51YY37 pKa = 10.65LYY39 pKa = 11.0NNPNEE44 pKa = 3.8ILEE47 pKa = 3.94YY48 pKa = 11.1DD49 pKa = 3.47NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0RUG0|A0A6C0RUG0_9CAUD Uncharacterized protein OS=Staphylococcus phage SA75 OX=2681195 PE=4 SV=1
MM1 pKa = 7.32NKK3 pKa = 9.57ILIRR7 pKa = 11.84FAINYY12 pKa = 8.84IKK14 pKa = 10.44YY15 pKa = 8.01QQKK18 pKa = 8.41QLRR21 pKa = 11.84EE22 pKa = 3.59KK23 pKa = 9.95EE24 pKa = 3.86ARR26 pKa = 11.84IKK28 pKa = 10.67YY29 pKa = 10.51LEE31 pKa = 4.46GFLKK35 pKa = 10.83GKK37 pKa = 10.62GYY39 pKa = 11.17

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13396

39

1154

206.1

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.726 ± 0.533

0.523 ± 0.084

6.3 ± 0.414

7.846 ± 0.573

4.345 ± 0.168

5.502 ± 0.298

1.56 ± 0.16

7.226 ± 0.31

9.361 ± 0.392

7.532 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.141

6.875 ± 0.235

2.822 ± 0.195

3.949 ± 0.205

4.076 ± 0.262

5.77 ± 0.275

6.263 ± 0.287

6.173 ± 0.295

1.165 ± 0.199

4.419 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski