Amino acid dipepetide frequency for Staphylococcus phage SA75

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.493AlaAla: 1.493 ± 0.462
0.373AlaCys: 0.373 ± 0.155
2.986AlaAsp: 2.986 ± 0.394
4.255AlaGlu: 4.255 ± 0.497
2.762AlaPhe: 2.762 ± 0.454
2.986AlaGly: 2.986 ± 0.422
1.045AlaHis: 1.045 ± 0.309
5.002AlaIle: 5.002 ± 0.978
4.853AlaLys: 4.853 ± 0.713
3.882AlaLeu: 3.882 ± 0.716
1.866AlaMet: 1.866 ± 0.499
3.658AlaAsn: 3.658 ± 0.578
2.016AlaPro: 2.016 ± 0.356
2.464AlaGln: 2.464 ± 0.42
2.762AlaArg: 2.762 ± 0.492
3.658AlaSer: 3.658 ± 0.583
4.255AlaThr: 4.255 ± 0.714
3.359AlaVal: 3.359 ± 0.555
0.821AlaTrp: 0.821 ± 0.32
2.762AlaTyr: 2.762 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
0.149CysAla: 0.149 ± 0.094
0.0CysCys: 0.0 ± 0.0
0.224CysAsp: 0.224 ± 0.178
0.373CysGlu: 0.373 ± 0.197
0.373CysPhe: 0.373 ± 0.166
0.149CysGly: 0.149 ± 0.098
0.0CysHis: 0.0 ± 0.0
0.149CysIle: 0.149 ± 0.106
0.597CysLys: 0.597 ± 0.189
0.373CysLeu: 0.373 ± 0.208
0.075CysMet: 0.075 ± 0.08
0.448CysAsn: 0.448 ± 0.223
0.373CysPro: 0.373 ± 0.2
0.224CysGln: 0.224 ± 0.116
0.224CysArg: 0.224 ± 0.129
0.523CysSer: 0.523 ± 0.237
0.373CysThr: 0.373 ± 0.149
0.224CysVal: 0.224 ± 0.139
0.149CysTrp: 0.149 ± 0.113
0.224CysTyr: 0.224 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
3.285AspAla: 3.285 ± 0.595
0.373AspCys: 0.373 ± 0.164
4.181AspAsp: 4.181 ± 0.772
6.047AspGlu: 6.047 ± 0.865
3.807AspPhe: 3.807 ± 0.504
3.733AspGly: 3.733 ± 0.614
0.224AspHis: 0.224 ± 0.208
4.853AspIle: 4.853 ± 0.608
5.226AspLys: 5.226 ± 0.666
5.151AspLeu: 5.151 ± 0.675
1.866AspMet: 1.866 ± 0.392
3.882AspAsn: 3.882 ± 0.535
1.493AspPro: 1.493 ± 0.362
1.418AspGln: 1.418 ± 0.399
2.389AspArg: 2.389 ± 0.447
4.031AspSer: 4.031 ± 0.522
3.434AspThr: 3.434 ± 0.581
4.255AspVal: 4.255 ± 0.52
0.672AspTrp: 0.672 ± 0.201
2.613AspTyr: 2.613 ± 0.576
0.0AspXaa: 0.0 ± 0.0
Glu
6.047GluAla: 6.047 ± 0.8
0.373GluCys: 0.373 ± 0.17
3.434GluAsp: 3.434 ± 0.539
5.748GluGlu: 5.748 ± 0.818
3.882GluPhe: 3.882 ± 0.619
2.762GluGly: 2.762 ± 0.542
1.12GluHis: 1.12 ± 0.254
6.271GluIle: 6.271 ± 1.011
6.122GluLys: 6.122 ± 0.858
7.167GluLeu: 7.167 ± 1.004
2.09GluMet: 2.09 ± 0.425
5.226GluAsn: 5.226 ± 0.81
1.717GluPro: 1.717 ± 0.371
3.733GluGln: 3.733 ± 0.473
3.957GluArg: 3.957 ± 0.719
3.733GluSer: 3.733 ± 0.705
3.21GluThr: 3.21 ± 0.494
5.599GluVal: 5.599 ± 0.674
0.747GluTrp: 0.747 ± 0.25
5.524GluTyr: 5.524 ± 0.648
0.0GluXaa: 0.0 ± 0.0
Phe
2.165PheAla: 2.165 ± 0.356
0.299PheCys: 0.299 ± 0.144
2.912PheAsp: 2.912 ± 0.443
3.882PheGlu: 3.882 ± 0.583
1.344PhePhe: 1.344 ± 0.279
3.061PheGly: 3.061 ± 0.47
0.672PheHis: 0.672 ± 0.227
3.061PheIle: 3.061 ± 0.425
4.778PheLys: 4.778 ± 0.571
3.061PheLeu: 3.061 ± 0.451
0.896PheMet: 0.896 ± 0.245
3.434PheAsn: 3.434 ± 0.484
0.747PhePro: 0.747 ± 0.215
1.493PheGln: 1.493 ± 0.408
1.493PheArg: 1.493 ± 0.343
3.135PheSer: 3.135 ± 0.515
2.912PheThr: 2.912 ± 0.479
2.24PheVal: 2.24 ± 0.481
0.224PheTrp: 0.224 ± 0.116
1.642PheTyr: 1.642 ± 0.32
0.0PheXaa: 0.0 ± 0.0
Gly
2.912GlyAla: 2.912 ± 0.517
0.448GlyCys: 0.448 ± 0.185
3.285GlyAsp: 3.285 ± 0.552
3.509GlyGlu: 3.509 ± 0.566
2.389GlyPhe: 2.389 ± 0.469
2.986GlyGly: 2.986 ± 0.492
1.344GlyHis: 1.344 ± 0.381
4.181GlyIle: 4.181 ± 0.537
4.554GlyLys: 4.554 ± 0.638
5.3GlyLeu: 5.3 ± 0.622
1.269GlyMet: 1.269 ± 0.278
3.061GlyAsn: 3.061 ± 0.453
0.597GlyPro: 0.597 ± 0.294
1.493GlyGln: 1.493 ± 0.309
2.09GlyArg: 2.09 ± 0.431
3.21GlySer: 3.21 ± 0.493
3.957GlyThr: 3.957 ± 0.586
4.479GlyVal: 4.479 ± 0.49
1.194GlyTrp: 1.194 ± 0.45
2.762GlyTyr: 2.762 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
1.344HisAla: 1.344 ± 0.317
0.075HisCys: 0.075 ± 0.076
0.971HisAsp: 0.971 ± 0.242
1.12HisGlu: 1.12 ± 0.301
0.971HisPhe: 0.971 ± 0.273
1.269HisGly: 1.269 ± 0.294
0.448HisHis: 0.448 ± 0.168
1.269HisIle: 1.269 ± 0.43
0.672HisLys: 0.672 ± 0.208
1.045HisLeu: 1.045 ± 0.302
0.448HisMet: 0.448 ± 0.21
1.12HisAsn: 1.12 ± 0.259
0.523HisPro: 0.523 ± 0.161
0.224HisGln: 0.224 ± 0.165
0.448HisArg: 0.448 ± 0.181
0.896HisSer: 0.896 ± 0.249
1.045HisThr: 1.045 ± 0.287
1.194HisVal: 1.194 ± 0.268
0.0HisTrp: 0.0 ± 0.0
0.523HisTyr: 0.523 ± 0.289
0.0HisXaa: 0.0 ± 0.0
Ile
4.703IleAla: 4.703 ± 0.709
0.149IleCys: 0.149 ± 0.13
6.047IleAsp: 6.047 ± 0.714
5.898IleGlu: 5.898 ± 0.711
2.538IlePhe: 2.538 ± 0.455
3.583IleGly: 3.583 ± 0.523
1.418IleHis: 1.418 ± 0.277
4.33IleIle: 4.33 ± 0.487
7.391IleLys: 7.391 ± 0.641
4.405IleLeu: 4.405 ± 0.455
1.866IleMet: 1.866 ± 0.342
4.255IleAsn: 4.255 ± 0.517
2.389IlePro: 2.389 ± 0.384
2.24IleGln: 2.24 ± 0.399
3.359IleArg: 3.359 ± 0.533
4.181IleSer: 4.181 ± 0.479
5.151IleThr: 5.151 ± 0.653
4.255IleVal: 4.255 ± 0.605
1.269IleTrp: 1.269 ± 0.623
2.837IleTyr: 2.837 ± 0.478
0.0IleXaa: 0.0 ± 0.0
Lys
5.823LysAla: 5.823 ± 0.693
0.299LysCys: 0.299 ± 0.212
6.271LysAsp: 6.271 ± 0.666
8.436LysGlu: 8.436 ± 1.132
3.285LysPhe: 3.285 ± 0.457
5.674LysGly: 5.674 ± 0.649
1.568LysHis: 1.568 ± 0.416
6.122LysIle: 6.122 ± 0.709
8.287LysLys: 8.287 ± 0.734
6.57LysLeu: 6.57 ± 0.752
2.24LysMet: 2.24 ± 0.403
5.375LysAsn: 5.375 ± 0.802
2.613LysPro: 2.613 ± 0.497
5.151LysGln: 5.151 ± 0.533
3.957LysArg: 3.957 ± 0.534
5.45LysSer: 5.45 ± 0.523
5.3LysThr: 5.3 ± 0.591
6.047LysVal: 6.047 ± 0.558
0.523LysTrp: 0.523 ± 0.179
4.629LysTyr: 4.629 ± 0.835
0.0LysXaa: 0.0 ± 0.0
Leu
3.135LeuAla: 3.135 ± 0.467
0.448LeuCys: 0.448 ± 0.232
4.554LeuAsp: 4.554 ± 0.522
5.972LeuGlu: 5.972 ± 0.718
3.509LeuPhe: 3.509 ± 0.534
3.733LeuGly: 3.733 ± 0.534
0.821LeuHis: 0.821 ± 0.257
4.405LeuIle: 4.405 ± 0.488
7.913LeuLys: 7.913 ± 0.708
5.375LeuLeu: 5.375 ± 0.636
1.642LeuMet: 1.642 ± 0.339
5.898LeuAsn: 5.898 ± 0.474
2.538LeuPro: 2.538 ± 0.404
3.509LeuGln: 3.509 ± 0.592
3.434LeuArg: 3.434 ± 0.685
4.853LeuSer: 4.853 ± 0.537
5.077LeuThr: 5.077 ± 0.646
4.778LeuVal: 4.778 ± 0.814
0.672LeuTrp: 0.672 ± 0.231
3.061LeuTyr: 3.061 ± 0.529
0.0LeuXaa: 0.0 ± 0.0
Met
1.344MetAla: 1.344 ± 0.275
0.075MetCys: 0.075 ± 0.076
1.12MetAsp: 1.12 ± 0.241
1.866MetGlu: 1.866 ± 0.404
0.896MetPhe: 0.896 ± 0.239
1.045MetGly: 1.045 ± 0.294
0.523MetHis: 0.523 ± 0.206
1.568MetIle: 1.568 ± 0.361
2.24MetLys: 2.24 ± 0.443
2.762MetLeu: 2.762 ± 0.414
0.747MetMet: 0.747 ± 0.225
2.165MetAsn: 2.165 ± 0.396
0.821MetPro: 0.821 ± 0.237
1.194MetGln: 1.194 ± 0.296
0.821MetArg: 0.821 ± 0.299
1.344MetSer: 1.344 ± 0.325
2.688MetThr: 2.688 ± 0.577
1.045MetVal: 1.045 ± 0.255
0.448MetTrp: 0.448 ± 0.181
0.971MetTyr: 0.971 ± 0.274
0.0MetXaa: 0.0 ± 0.0
Asn
4.927AsnAla: 4.927 ± 0.828
0.448AsnCys: 0.448 ± 0.267
4.853AsnAsp: 4.853 ± 0.551
4.778AsnGlu: 4.778 ± 0.64
2.912AsnPhe: 2.912 ± 0.45
4.778AsnGly: 4.778 ± 0.613
0.821AsnHis: 0.821 ± 0.227
3.957AsnIle: 3.957 ± 0.512
7.167AsnLys: 7.167 ± 0.661
4.703AsnLeu: 4.703 ± 0.633
1.568AsnMet: 1.568 ± 0.316
5.002AsnAsn: 5.002 ± 0.876
2.762AsnPro: 2.762 ± 0.513
2.389AsnGln: 2.389 ± 0.386
2.464AsnArg: 2.464 ± 0.352
3.285AsnSer: 3.285 ± 0.531
4.629AsnThr: 4.629 ± 0.589
3.807AsnVal: 3.807 ± 0.555
0.896AsnTrp: 0.896 ± 0.204
2.613AsnTyr: 2.613 ± 0.521
0.0AsnXaa: 0.0 ± 0.0
Pro
1.269ProAla: 1.269 ± 0.284
0.224ProCys: 0.224 ± 0.109
1.344ProAsp: 1.344 ± 0.325
2.09ProGlu: 2.09 ± 0.344
1.269ProPhe: 1.269 ± 0.318
1.941ProGly: 1.941 ± 0.457
0.448ProHis: 0.448 ± 0.178
2.165ProIle: 2.165 ± 0.393
3.434ProLys: 3.434 ± 0.601
1.717ProLeu: 1.717 ± 0.337
1.194ProMet: 1.194 ± 0.288
2.538ProAsn: 2.538 ± 0.365
0.523ProPro: 0.523 ± 0.249
1.269ProGln: 1.269 ± 0.358
0.821ProArg: 0.821 ± 0.275
1.269ProSer: 1.269 ± 0.371
1.792ProThr: 1.792 ± 0.399
1.866ProVal: 1.866 ± 0.501
0.075ProTrp: 0.075 ± 0.087
0.971ProTyr: 0.971 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
2.837GlnAla: 2.837 ± 0.506
0.299GlnCys: 0.299 ± 0.168
2.464GlnAsp: 2.464 ± 0.472
2.389GlnGlu: 2.389 ± 0.563
1.568GlnPhe: 1.568 ± 0.309
2.24GlnGly: 2.24 ± 0.356
0.672GlnHis: 0.672 ± 0.196
2.314GlnIle: 2.314 ± 0.391
3.733GlnLys: 3.733 ± 0.572
3.135GlnLeu: 3.135 ± 0.602
1.418GlnMet: 1.418 ± 0.335
2.389GlnAsn: 2.389 ± 0.294
1.792GlnPro: 1.792 ± 0.426
2.24GlnGln: 2.24 ± 0.558
1.642GlnArg: 1.642 ± 0.334
2.09GlnSer: 2.09 ± 0.462
2.09GlnThr: 2.09 ± 0.331
2.24GlnVal: 2.24 ± 0.428
0.448GlnTrp: 0.448 ± 0.181
1.493GlnTyr: 1.493 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
1.418ArgAla: 1.418 ± 0.307
0.373ArgCys: 0.373 ± 0.164
2.016ArgAsp: 2.016 ± 0.432
3.359ArgGlu: 3.359 ± 0.491
2.09ArgPhe: 2.09 ± 0.362
1.941ArgGly: 1.941 ± 0.385
1.045ArgHis: 1.045 ± 0.305
3.583ArgIle: 3.583 ± 0.568
3.957ArgLys: 3.957 ± 0.606
3.882ArgLeu: 3.882 ± 0.436
0.523ArgMet: 0.523 ± 0.194
2.986ArgAsn: 2.986 ± 0.502
1.045ArgPro: 1.045 ± 0.25
1.642ArgGln: 1.642 ± 0.379
1.866ArgArg: 1.866 ± 0.42
2.389ArgSer: 2.389 ± 0.383
2.09ArgThr: 2.09 ± 0.486
2.09ArgVal: 2.09 ± 0.494
0.448ArgTrp: 0.448 ± 0.232
2.016ArgTyr: 2.016 ± 0.465
0.0ArgXaa: 0.0 ± 0.0
Ser
4.33SerAla: 4.33 ± 0.772
0.299SerCys: 0.299 ± 0.17
4.703SerAsp: 4.703 ± 0.867
3.807SerGlu: 3.807 ± 0.525
2.613SerPhe: 2.613 ± 0.485
3.135SerGly: 3.135 ± 0.423
0.971SerHis: 0.971 ± 0.376
4.927SerIle: 4.927 ± 0.563
5.45SerLys: 5.45 ± 0.654
3.658SerLeu: 3.658 ± 0.412
1.792SerMet: 1.792 ± 0.407
4.255SerAsn: 4.255 ± 0.463
0.971SerPro: 0.971 ± 0.332
1.941SerGln: 1.941 ± 0.365
2.613SerArg: 2.613 ± 0.346
3.135SerSer: 3.135 ± 0.486
3.21SerThr: 3.21 ± 0.379
3.061SerVal: 3.061 ± 0.481
0.299SerTrp: 0.299 ± 0.123
2.538SerTyr: 2.538 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
3.807ThrAla: 3.807 ± 0.537
0.0ThrCys: 0.0 ± 0.0
3.807ThrAsp: 3.807 ± 0.719
4.479ThrGlu: 4.479 ± 0.753
2.986ThrPhe: 2.986 ± 0.423
4.106ThrGly: 4.106 ± 0.529
0.672ThrHis: 0.672 ± 0.176
5.524ThrIle: 5.524 ± 1.11
5.226ThrLys: 5.226 ± 0.608
5.151ThrLeu: 5.151 ± 0.536
0.971ThrMet: 0.971 ± 0.309
4.031ThrAsn: 4.031 ± 0.605
2.09ThrPro: 2.09 ± 0.333
2.762ThrGln: 2.762 ± 0.602
2.24ThrArg: 2.24 ± 0.336
3.807ThrSer: 3.807 ± 0.663
3.807ThrThr: 3.807 ± 0.706
4.181ThrVal: 4.181 ± 0.764
0.821ThrTrp: 0.821 ± 0.261
2.165ThrTyr: 2.165 ± 0.439
0.0ThrXaa: 0.0 ± 0.0
Val
3.509ValAla: 3.509 ± 0.868
0.373ValCys: 0.373 ± 0.158
4.853ValAsp: 4.853 ± 0.699
5.3ValGlu: 5.3 ± 0.771
2.464ValPhe: 2.464 ± 0.489
2.837ValGly: 2.837 ± 0.528
0.747ValHis: 0.747 ± 0.247
5.077ValIle: 5.077 ± 0.535
5.898ValLys: 5.898 ± 0.572
4.255ValLeu: 4.255 ± 0.645
1.642ValMet: 1.642 ± 0.387
4.853ValAsn: 4.853 ± 0.577
1.866ValPro: 1.866 ± 0.507
1.493ValGln: 1.493 ± 0.381
2.09ValArg: 2.09 ± 0.447
3.509ValSer: 3.509 ± 0.52
3.807ValThr: 3.807 ± 0.63
3.583ValVal: 3.583 ± 0.505
1.045ValTrp: 1.045 ± 0.344
2.538ValTyr: 2.538 ± 0.53
0.0ValXaa: 0.0 ± 0.0
Trp
0.971TrpAla: 0.971 ± 0.391
0.149TrpCys: 0.149 ± 0.13
0.448TrpAsp: 0.448 ± 0.174
0.821TrpGlu: 0.821 ± 0.255
0.299TrpPhe: 0.299 ± 0.157
0.523TrpGly: 0.523 ± 0.328
0.224TrpHis: 0.224 ± 0.124
0.597TrpIle: 0.597 ± 0.244
0.971TrpLys: 0.971 ± 0.247
0.747TrpLeu: 0.747 ± 0.249
0.149TrpMet: 0.149 ± 0.101
1.642TrpAsn: 1.642 ± 0.94
0.149TrpPro: 0.149 ± 0.101
0.747TrpGln: 0.747 ± 0.251
0.224TrpArg: 0.224 ± 0.132
0.672TrpSer: 0.672 ± 0.267
1.045TrpThr: 1.045 ± 0.27
0.672TrpVal: 0.672 ± 0.268
0.0TrpTrp: 0.0 ± 0.0
0.597TrpTyr: 0.597 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.792TyrAla: 1.792 ± 0.317
0.149TyrCys: 0.149 ± 0.107
2.538TyrAsp: 2.538 ± 0.441
4.33TyrGlu: 4.33 ± 0.613
1.493TyrPhe: 1.493 ± 0.348
2.538TyrGly: 2.538 ± 0.559
0.821TyrHis: 0.821 ± 0.271
2.912TyrIle: 2.912 ± 0.554
5.151TyrLys: 5.151 ± 0.601
2.986TyrLeu: 2.986 ± 0.6
1.344TyrMet: 1.344 ± 0.277
2.538TyrAsn: 2.538 ± 0.327
1.194TyrPro: 1.194 ± 0.284
1.866TyrGln: 1.866 ± 0.332
1.866TyrArg: 1.866 ± 0.419
2.538TyrSer: 2.538 ± 0.451
2.762TyrThr: 2.762 ± 0.369
2.762TyrVal: 2.762 ± 0.45
0.896TyrTrp: 0.896 ± 0.256
1.717TyrTyr: 1.717 ± 0.302
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13396 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski