Synechococcus phage ACG-2014c
Average proteome isoelectric point is 5.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H8ZMG6|H8ZMG6_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014c OX=1079998 GN=Syn7803C43_59 PE=4 SV=1
MM1 pKa = 7.8 NYY3 pKa = 8.67 TLKK6 pKa = 10.5 QLQDD10 pKa = 3.5 RR11 pKa = 11.84 VSSMIKK17 pKa = 9.92 EE18 pKa = 4.01 QGEE21 pKa = 4.25 DD22 pKa = 3.62 AEE24 pKa = 4.52 CAAWIYY30 pKa = 9.2 TKK32 pKa = 10.79 NDD34 pKa = 2.97 CHH36 pKa = 7.97 LKK38 pKa = 10.75 DD39 pKa = 4.03 EE40 pKa = 4.77 NGEE43 pKa = 3.67 TDD45 pKa = 3.45 YY46 pKa = 11.61 QNNVEE51 pKa = 4.44 DD52 pKa = 4.67 PEE54 pKa = 4.24 VLARR58 pKa = 11.84 IFDD61 pKa = 4.0 DD62 pKa = 4.01 VGNIDD67 pKa = 4.07 YY68 pKa = 9.95 IYY70 pKa = 10.8 EE71 pKa = 4.31 VIQDD75 pKa = 3.76 AVDD78 pKa = 3.51 EE79 pKa = 4.48 VTEE82 pKa = 4.15 EE83 pKa = 4.24 QLVQYY88 pKa = 8.37 QQEE91 pKa = 4.23 LVEE94 pKa = 4.68 VKK96 pKa = 10.58
Molecular weight: 11.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|H8ZMU3|H8ZMU3_9CAUD TalC transaldolase-like protein OS=Synechococcus phage ACG-2014c OX=1079998 GN=Syn7803C43_179 PE=4 SV=1
MM1 pKa = 6.85 NTLTIIKK8 pKa = 10.34 NKK10 pKa = 9.4 IEE12 pKa = 3.96 KK13 pKa = 10.03 AARR16 pKa = 11.84 LHH18 pKa = 6.55 DD19 pKa = 4.17 AQILMTTYY27 pKa = 10.37 RR28 pKa = 11.84 GVKK31 pKa = 6.91 YY32 pKa = 9.08 TCDD35 pKa = 3.19 HH36 pKa = 6.74 SGDD39 pKa = 4.09 SVHH42 pKa = 6.61 GTFCYY47 pKa = 9.9 RR48 pKa = 11.84 GRR50 pKa = 11.84 TYY52 pKa = 11.14 KK53 pKa = 10.63 KK54 pKa = 10.25
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 9.18
IPC_protein 9.165
Toseland 9.692
ProMoST 9.604
Dawson 9.955
Bjellqvist 9.692
Wikipedia 10.145
Rodwell 10.277
Grimsley 10.043
Solomon 10.014
Lehninger 9.984
Nozaki 9.794
DTASelect 9.663
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.897
Patrickios 7.527
IPC_peptide 10.014
IPC2_peptide 8.536
IPC2.peptide.svr19 8.104
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
225
0
225
55948
35
7022
248.7
27.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.822 ± 0.189
0.965 ± 0.107
6.874 ± 0.141
5.979 ± 0.243
4.408 ± 0.089
7.918 ± 0.318
1.362 ± 0.117
6.604 ± 0.237
5.791 ± 0.424
7.067 ± 0.148
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.102 ± 0.241
6.022 ± 0.182
3.877 ± 0.122
3.56 ± 0.076
3.829 ± 0.149
7.303 ± 0.299
7.516 ± 0.397
6.653 ± 0.178
1.133 ± 0.094
4.215 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here