Amino acid dipepetide frequency for Synechococcus phage ACG-2014c

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.863AlaAla: 5.863 ± 0.543
0.643AlaCys: 0.643 ± 0.119
4.397AlaAsp: 4.397 ± 0.272
3.986AlaGlu: 3.986 ± 0.309
2.627AlaPhe: 2.627 ± 0.242
5.952AlaGly: 5.952 ± 0.512
0.912AlaHis: 0.912 ± 0.147
4.075AlaIle: 4.075 ± 0.41
3.861AlaLys: 3.861 ± 0.451
4.772AlaLeu: 4.772 ± 0.37
1.18AlaMet: 1.18 ± 0.208
4.111AlaAsn: 4.111 ± 0.346
2.77AlaPro: 2.77 ± 0.222
2.306AlaGln: 2.306 ± 0.221
2.735AlaArg: 2.735 ± 0.229
5.326AlaSer: 5.326 ± 0.374
5.487AlaThr: 5.487 ± 0.451
4.218AlaVal: 4.218 ± 0.292
0.572AlaTrp: 0.572 ± 0.105
2.431AlaTyr: 2.431 ± 0.217
0.0AlaXaa: 0.0 ± 0.0
Cys
0.626CysAla: 0.626 ± 0.111
0.107CysCys: 0.107 ± 0.047
0.715CysAsp: 0.715 ± 0.145
0.572CysGlu: 0.572 ± 0.128
0.465CysPhe: 0.465 ± 0.109
0.643CysGly: 0.643 ± 0.122
0.25CysHis: 0.25 ± 0.086
0.697CysIle: 0.697 ± 0.154
0.643CysLys: 0.643 ± 0.123
0.59CysLeu: 0.59 ± 0.115
0.322CysMet: 0.322 ± 0.097
0.429CysAsn: 0.429 ± 0.111
0.34CysPro: 0.34 ± 0.1
0.375CysGln: 0.375 ± 0.078
0.465CysArg: 0.465 ± 0.092
0.572CysSer: 0.572 ± 0.098
0.59CysThr: 0.59 ± 0.111
0.733CysVal: 0.733 ± 0.141
0.071CysTrp: 0.071 ± 0.04
0.447CysTyr: 0.447 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
5.416AspAla: 5.416 ± 0.345
0.769AspCys: 0.769 ± 0.136
5.13AspAsp: 5.13 ± 0.459
4.129AspGlu: 4.129 ± 0.341
3.074AspPhe: 3.074 ± 0.219
6.292AspGly: 6.292 ± 0.41
0.912AspHis: 0.912 ± 0.146
4.451AspIle: 4.451 ± 0.318
3.164AspLys: 3.164 ± 0.316
4.915AspLeu: 4.915 ± 0.298
1.519AspMet: 1.519 ± 0.175
3.325AspAsn: 3.325 ± 0.208
3.253AspPro: 3.253 ± 0.26
2.038AspGln: 2.038 ± 0.212
2.324AspArg: 2.324 ± 0.226
4.772AspSer: 4.772 ± 0.305
4.701AspThr: 4.701 ± 0.336
4.29AspVal: 4.29 ± 0.355
0.912AspTrp: 0.912 ± 0.138
3.342AspTyr: 3.342 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
3.45GluAla: 3.45 ± 0.24
0.643GluCys: 0.643 ± 0.138
4.272GluAsp: 4.272 ± 0.364
4.433GluGlu: 4.433 ± 0.54
3.074GluPhe: 3.074 ± 0.218
4.147GluGly: 4.147 ± 0.29
0.554GluHis: 0.554 ± 0.107
4.594GluIle: 4.594 ± 0.375
3.932GluLys: 3.932 ± 0.422
4.165GluLeu: 4.165 ± 0.274
1.77GluMet: 1.77 ± 0.329
3.36GluAsn: 3.36 ± 0.256
1.108GluPro: 1.108 ± 0.134
1.895GluGln: 1.895 ± 0.217
2.288GluArg: 2.288 ± 0.274
4.004GluSer: 4.004 ± 0.293
3.879GluThr: 3.879 ± 0.332
4.737GluVal: 4.737 ± 0.249
0.643GluTrp: 0.643 ± 0.132
2.842GluTyr: 2.842 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
3.039PheAla: 3.039 ± 0.245
0.554PheCys: 0.554 ± 0.109
3.807PheAsp: 3.807 ± 0.26
2.395PheGlu: 2.395 ± 0.215
1.93PhePhe: 1.93 ± 0.197
3.182PheGly: 3.182 ± 0.246
0.661PheHis: 0.661 ± 0.143
2.896PheIle: 2.896 ± 0.222
2.342PheLys: 2.342 ± 0.305
2.788PheLeu: 2.788 ± 0.31
1.233PheMet: 1.233 ± 0.208
2.788PheAsn: 2.788 ± 0.197
1.609PhePro: 1.609 ± 0.216
1.519PheGln: 1.519 ± 0.137
1.501PheArg: 1.501 ± 0.168
3.396PheSer: 3.396 ± 0.244
3.468PheThr: 3.468 ± 0.253
2.949PheVal: 2.949 ± 0.275
0.34PheTrp: 0.34 ± 0.085
1.68PheTyr: 1.68 ± 0.172
0.0PheXaa: 0.0 ± 0.0
Gly
5.97GlyAla: 5.97 ± 0.443
0.59GlyCys: 0.59 ± 0.111
5.38GlyAsp: 5.38 ± 0.49
4.486GlyGlu: 4.486 ± 0.269
3.271GlyPhe: 3.271 ± 0.251
7.918GlyGly: 7.918 ± 0.875
0.983GlyHis: 0.983 ± 0.152
4.808GlyIle: 4.808 ± 0.321
4.075GlyLys: 4.075 ± 0.373
4.397GlyLeu: 4.397 ± 0.323
1.609GlyMet: 1.609 ± 0.242
4.826GlyAsn: 4.826 ± 0.399
2.27GlyPro: 2.27 ± 0.285
2.824GlyGln: 2.824 ± 0.199
2.842GlyArg: 2.842 ± 0.276
6.56GlySer: 6.56 ± 0.841
6.989GlyThr: 6.989 ± 0.59
4.683GlyVal: 4.683 ± 0.315
1.162GlyTrp: 1.162 ± 0.145
3.539GlyTyr: 3.539 ± 0.264
0.0GlyXaa: 0.0 ± 0.0
His
0.518HisAla: 0.518 ± 0.123
0.179HisCys: 0.179 ± 0.061
0.858HisAsp: 0.858 ± 0.136
0.947HisGlu: 0.947 ± 0.179
0.894HisPhe: 0.894 ± 0.147
0.858HisGly: 0.858 ± 0.127
0.25HisHis: 0.25 ± 0.085
1.037HisIle: 1.037 ± 0.157
0.804HisLys: 0.804 ± 0.153
0.858HisLeu: 0.858 ± 0.123
0.34HisMet: 0.34 ± 0.1
0.626HisAsn: 0.626 ± 0.113
0.786HisPro: 0.786 ± 0.129
0.536HisGln: 0.536 ± 0.099
0.661HisArg: 0.661 ± 0.15
0.769HisSer: 0.769 ± 0.118
1.001HisThr: 1.001 ± 0.143
0.786HisVal: 0.786 ± 0.133
0.179HisTrp: 0.179 ± 0.071
0.733HisTyr: 0.733 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
4.129IleAla: 4.129 ± 0.307
0.661IleCys: 0.661 ± 0.135
5.309IleAsp: 5.309 ± 0.313
4.361IleGlu: 4.361 ± 0.296
3.003IlePhe: 3.003 ± 0.235
4.737IleGly: 4.737 ± 0.338
0.715IleHis: 0.715 ± 0.12
3.932IleIle: 3.932 ± 0.305
4.004IleLys: 4.004 ± 0.266
4.719IleLeu: 4.719 ± 0.393
0.894IleMet: 0.894 ± 0.171
4.236IleAsn: 4.236 ± 0.281
2.556IlePro: 2.556 ± 0.208
2.842IleGln: 2.842 ± 0.259
2.52IleArg: 2.52 ± 0.218
4.915IleSer: 4.915 ± 0.528
5.416IleThr: 5.416 ± 0.586
4.343IleVal: 4.343 ± 0.512
0.5IleTrp: 0.5 ± 0.098
2.252IleTyr: 2.252 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
2.913LysAla: 2.913 ± 0.306
0.679LysCys: 0.679 ± 0.143
3.45LysAsp: 3.45 ± 0.325
3.771LysGlu: 3.771 ± 0.414
2.663LysPhe: 2.663 ± 0.28
3.414LysGly: 3.414 ± 0.382
0.965LysHis: 0.965 ± 0.202
4.504LysIle: 4.504 ± 0.309
4.522LysLys: 4.522 ± 0.607
4.379LysLeu: 4.379 ± 0.319
1.394LysMet: 1.394 ± 0.214
3.092LysAsn: 3.092 ± 0.279
2.02LysPro: 2.02 ± 0.267
2.109LysGln: 2.109 ± 0.288
2.413LysArg: 2.413 ± 0.265
4.004LysSer: 4.004 ± 0.345
3.718LysThr: 3.718 ± 0.298
4.308LysVal: 4.308 ± 0.357
0.84LysTrp: 0.84 ± 0.146
2.753LysTyr: 2.753 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
4.379LeuAla: 4.379 ± 0.387
0.804LeuCys: 0.804 ± 0.155
5.523LeuAsp: 5.523 ± 0.318
4.165LeuGlu: 4.165 ± 0.266
2.645LeuPhe: 2.645 ± 0.254
4.218LeuGly: 4.218 ± 0.338
1.198LeuHis: 1.198 ± 0.207
4.057LeuIle: 4.057 ± 0.265
4.808LeuLys: 4.808 ± 0.414
5.166LeuLeu: 5.166 ± 0.431
1.394LeuMet: 1.394 ± 0.254
5.076LeuAsn: 5.076 ± 0.347
3.128LeuPro: 3.128 ± 0.209
2.824LeuGln: 2.824 ± 0.229
3.039LeuArg: 3.039 ± 0.232
4.969LeuSer: 4.969 ± 0.346
5.166LeuThr: 5.166 ± 0.456
4.343LeuVal: 4.343 ± 0.3
0.697LeuTrp: 0.697 ± 0.138
3.074LeuTyr: 3.074 ± 0.235
0.0LeuXaa: 0.0 ± 0.0
Met
1.43MetAla: 1.43 ± 0.207
0.268MetCys: 0.268 ± 0.079
1.126MetAsp: 1.126 ± 0.177
1.341MetGlu: 1.341 ± 0.22
0.822MetPhe: 0.822 ± 0.164
1.323MetGly: 1.323 ± 0.214
0.34MetHis: 0.34 ± 0.1
1.269MetIle: 1.269 ± 0.205
2.073MetLys: 2.073 ± 0.284
1.662MetLeu: 1.662 ± 0.273
0.572MetMet: 0.572 ± 0.134
1.215MetAsn: 1.215 ± 0.212
1.001MetPro: 1.001 ± 0.168
0.715MetGln: 0.715 ± 0.148
0.929MetArg: 0.929 ± 0.162
1.394MetSer: 1.394 ± 0.224
1.68MetThr: 1.68 ± 0.232
0.983MetVal: 0.983 ± 0.144
0.393MetTrp: 0.393 ± 0.09
0.483MetTyr: 0.483 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
4.308AsnAla: 4.308 ± 0.336
0.483AsnCys: 0.483 ± 0.102
3.396AsnAsp: 3.396 ± 0.244
3.36AsnGlu: 3.36 ± 0.22
2.699AsnPhe: 2.699 ± 0.229
4.844AsnGly: 4.844 ± 0.505
0.84AsnHis: 0.84 ± 0.143
4.486AsnIle: 4.486 ± 0.356
3.128AsnLys: 3.128 ± 0.296
4.862AsnLeu: 4.862 ± 0.281
1.001AsnMet: 1.001 ± 0.196
3.611AsnAsn: 3.611 ± 0.346
3.039AsnPro: 3.039 ± 0.188
1.966AsnGln: 1.966 ± 0.178
2.395AsnArg: 2.395 ± 0.183
3.861AsnSer: 3.861 ± 0.415
4.469AsnThr: 4.469 ± 0.349
4.343AsnVal: 4.343 ± 0.295
0.697AsnTrp: 0.697 ± 0.123
2.431AsnTyr: 2.431 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
2.556ProAla: 2.556 ± 0.222
0.429ProCys: 0.429 ± 0.122
2.753ProAsp: 2.753 ± 0.307
2.788ProGlu: 2.788 ± 0.245
1.68ProPhe: 1.68 ± 0.179
3.164ProGly: 3.164 ± 0.277
0.697ProHis: 0.697 ± 0.094
2.109ProIle: 2.109 ± 0.193
2.306ProLys: 2.306 ± 0.303
2.056ProLeu: 2.056 ± 0.17
0.679ProMet: 0.679 ± 0.149
2.109ProAsn: 2.109 ± 0.18
1.895ProPro: 1.895 ± 0.256
1.233ProGln: 1.233 ± 0.158
1.466ProArg: 1.466 ± 0.171
3.074ProSer: 3.074 ± 0.245
3.074ProThr: 3.074 ± 0.257
2.61ProVal: 2.61 ± 0.261
0.608ProTrp: 0.608 ± 0.105
1.484ProTyr: 1.484 ± 0.162
0.0ProXaa: 0.0 ± 0.0
Gln
1.984GlnAla: 1.984 ± 0.169
0.34GlnCys: 0.34 ± 0.079
2.109GlnAsp: 2.109 ± 0.168
2.199GlnGlu: 2.199 ± 0.174
1.948GlnPhe: 1.948 ± 0.231
2.413GlnGly: 2.413 ± 0.239
0.518GlnHis: 0.518 ± 0.1
2.52GlnIle: 2.52 ± 0.195
2.306GlnLys: 2.306 ± 0.314
3.199GlnLeu: 3.199 ± 0.236
0.822GlnMet: 0.822 ± 0.158
1.895GlnAsn: 1.895 ± 0.184
1.055GlnPro: 1.055 ± 0.131
1.323GlnGln: 1.323 ± 0.165
1.412GlnArg: 1.412 ± 0.178
2.502GlnSer: 2.502 ± 0.186
2.395GlnThr: 2.395 ± 0.244
2.449GlnVal: 2.449 ± 0.187
0.5GlnTrp: 0.5 ± 0.089
1.716GlnTyr: 1.716 ± 0.175
0.0GlnXaa: 0.0 ± 0.0
Arg
2.61ArgAla: 2.61 ± 0.249
0.357ArgCys: 0.357 ± 0.084
2.181ArgAsp: 2.181 ± 0.225
2.145ArgGlu: 2.145 ± 0.265
1.716ArgPhe: 1.716 ± 0.211
2.484ArgGly: 2.484 ± 0.23
0.733ArgHis: 0.733 ± 0.139
2.627ArgIle: 2.627 ± 0.235
2.484ArgLys: 2.484 ± 0.283
3.164ArgLeu: 3.164 ± 0.225
1.001ArgMet: 1.001 ± 0.168
2.395ArgAsn: 2.395 ± 0.231
1.126ArgPro: 1.126 ± 0.178
1.787ArgGln: 1.787 ± 0.18
1.895ArgArg: 1.895 ± 0.225
2.127ArgSer: 2.127 ± 0.169
1.93ArgThr: 1.93 ± 0.281
3.074ArgVal: 3.074 ± 0.194
0.465ArgTrp: 0.465 ± 0.1
1.984ArgTyr: 1.984 ± 0.196
0.0ArgXaa: 0.0 ± 0.0
Ser
5.38SerAla: 5.38 ± 0.34
0.465SerCys: 0.465 ± 0.103
4.629SerAsp: 4.629 ± 0.283
3.342SerGlu: 3.342 ± 0.277
3.289SerPhe: 3.289 ± 0.209
7.811SerGly: 7.811 ± 0.74
0.84SerHis: 0.84 ± 0.12
4.522SerIle: 4.522 ± 0.525
3.611SerLys: 3.611 ± 0.329
5.398SerLeu: 5.398 ± 0.287
1.537SerMet: 1.537 ± 0.223
4.808SerAsn: 4.808 ± 0.361
2.663SerPro: 2.663 ± 0.282
2.717SerGln: 2.717 ± 0.256
2.377SerArg: 2.377 ± 0.228
6.024SerSer: 6.024 ± 0.508
5.416SerThr: 5.416 ± 0.378
4.433SerVal: 4.433 ± 0.347
0.84SerTrp: 0.84 ± 0.162
2.931SerTyr: 2.931 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
5.863ThrAla: 5.863 ± 0.456
0.518ThrCys: 0.518 ± 0.087
3.914ThrAsp: 3.914 ± 0.311
3.932ThrGlu: 3.932 ± 0.296
3.396ThrPhe: 3.396 ± 0.358
7.132ThrGly: 7.132 ± 0.589
0.84ThrHis: 0.84 ± 0.138
5.362ThrIle: 5.362 ± 0.365
3.199ThrLys: 3.199 ± 0.283
6.292ThrLeu: 6.292 ± 0.531
1.198ThrMet: 1.198 ± 0.204
4.343ThrAsn: 4.343 ± 0.423
3.432ThrPro: 3.432 ± 0.269
2.484ThrGln: 2.484 ± 0.169
2.306ThrArg: 2.306 ± 0.194
5.755ThrSer: 5.755 ± 0.47
6.077ThrThr: 6.077 ± 0.671
5.344ThrVal: 5.344 ± 0.548
0.715ThrTrp: 0.715 ± 0.106
3.056ThrTyr: 3.056 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
4.665ValAla: 4.665 ± 0.319
0.536ValCys: 0.536 ± 0.099
5.166ValAsp: 5.166 ± 0.349
4.254ValGlu: 4.254 ± 0.25
2.556ValPhe: 2.556 ± 0.222
5.219ValGly: 5.219 ± 0.377
0.643ValHis: 0.643 ± 0.116
4.826ValIle: 4.826 ± 0.383
3.593ValLys: 3.593 ± 0.357
3.664ValLeu: 3.664 ± 0.265
1.305ValMet: 1.305 ± 0.172
4.183ValAsn: 4.183 ± 0.314
2.949ValPro: 2.949 ± 0.209
1.913ValGln: 1.913 ± 0.196
2.252ValArg: 2.252 ± 0.193
5.326ValSer: 5.326 ± 0.386
5.809ValThr: 5.809 ± 0.401
4.576ValVal: 4.576 ± 0.285
0.715ValTrp: 0.715 ± 0.115
2.377ValTyr: 2.377 ± 0.22
0.0ValXaa: 0.0 ± 0.0
Trp
0.733TrpAla: 0.733 ± 0.113
0.143TrpCys: 0.143 ± 0.06
0.912TrpAsp: 0.912 ± 0.132
0.804TrpGlu: 0.804 ± 0.171
0.536TrpPhe: 0.536 ± 0.1
0.554TrpGly: 0.554 ± 0.083
0.25TrpHis: 0.25 ± 0.088
0.608TrpIle: 0.608 ± 0.136
0.715TrpLys: 0.715 ± 0.126
0.697TrpLeu: 0.697 ± 0.146
0.322TrpMet: 0.322 ± 0.075
0.84TrpAsn: 0.84 ± 0.118
0.286TrpPro: 0.286 ± 0.079
0.554TrpGln: 0.554 ± 0.088
0.465TrpArg: 0.465 ± 0.095
0.822TrpSer: 0.822 ± 0.125
0.804TrpThr: 0.804 ± 0.104
0.715TrpVal: 0.715 ± 0.122
0.107TrpTrp: 0.107 ± 0.049
0.465TrpTyr: 0.465 ± 0.103
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.395TyrAla: 2.395 ± 0.228
0.483TyrCys: 0.483 ± 0.094
3.718TyrAsp: 3.718 ± 0.229
2.377TyrGlu: 2.377 ± 0.221
1.787TyrPhe: 1.787 ± 0.181
2.878TyrGly: 2.878 ± 0.215
0.518TyrHis: 0.518 ± 0.093
2.663TyrIle: 2.663 ± 0.198
2.342TyrLys: 2.342 ± 0.241
2.931TyrLeu: 2.931 ± 0.275
0.912TyrMet: 0.912 ± 0.128
2.949TyrAsn: 2.949 ± 0.257
1.484TyrPro: 1.484 ± 0.156
1.644TyrGln: 1.644 ± 0.172
2.002TyrArg: 2.002 ± 0.203
2.86TyrSer: 2.86 ± 0.301
3.092TyrThr: 3.092 ± 0.285
2.61TyrVal: 2.61 ± 0.235
0.375TyrTrp: 0.375 ± 0.101
2.127TyrTyr: 2.127 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 225 proteins (55948 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski