Yaba monkey tumor virus (strain VR587) (YMTV)
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6TUZ5|Q6TUZ5_YMTV5 16L OS=Yaba monkey tumor virus (strain VR587) OX=928314 PE=4 SV=1
MM1 pKa = 7.47 NSTINFEE8 pKa = 4.27 DD9 pKa = 3.99 SEE11 pKa = 4.68 FGANMVMVISVFVLMISFLVFMLLYY36 pKa = 10.06 LIKK39 pKa = 9.58 WSYY42 pKa = 11.41 VMGFLNDD49 pKa = 3.49 VKK51 pKa = 10.63 IKK53 pKa = 10.0 IMNMTTRR60 pKa = 11.84 RR61 pKa = 11.84 SFAHH65 pKa = 7.1 LDD67 pKa = 3.81 DD68 pKa = 4.65 IYY70 pKa = 10.47 YY71 pKa = 10.41 TSDD74 pKa = 3.49 DD75 pKa = 4.11 VIGLNVEE82 pKa = 4.12
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.459
IPC2_protein 4.571
IPC_protein 4.368
Toseland 4.19
ProMoST 4.406
Dawson 4.355
Bjellqvist 4.622
Wikipedia 4.291
Rodwell 4.202
Grimsley 4.101
Solomon 4.342
Lehninger 4.304
Nozaki 4.482
DTASelect 4.698
Thurlkill 4.24
EMBOSS 4.304
Sillero 4.495
Patrickios 3.592
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.456
Protein with the highest isoelectric point:
>tr|Q6TUW8|Q6TUW8_YMTV5 45L OS=Yaba monkey tumor virus (strain VR587) OX=928314 PE=4 SV=1
MM1 pKa = 7.52 ALSTKK6 pKa = 10.1 EE7 pKa = 3.65 IFSAIGITCMALLMIISGGALTFKK31 pKa = 10.71 SLAPHH36 pKa = 7.16 RR37 pKa = 11.84 IIVMRR42 pKa = 11.84 SATFNKK48 pKa = 9.37 ILSILEE54 pKa = 3.88 YY55 pKa = 10.06 VAILIFVPGTIALYY69 pKa = 9.93 SAYY72 pKa = 9.58 IKK74 pKa = 9.85 TLILGG79 pKa = 3.88
Molecular weight: 8.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.194
IPC_protein 9.224
Toseland 9.648
ProMoST 9.443
Dawson 9.926
Bjellqvist 9.648
Wikipedia 10.116
Rodwell 10.204
Grimsley 10.014
Solomon 9.999
Lehninger 9.97
Nozaki 9.706
DTASelect 9.633
Thurlkill 9.75
EMBOSS 10.058
Sillero 9.853
Patrickios 7.512
IPC_peptide 9.999
IPC2_peptide 8.463
IPC2.peptide.svr19 8.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
140
0
140
42362
44
1895
302.6
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.743 ± 0.12
2.231 ± 0.12
5.8 ± 0.132
5.278 ± 0.143
5.913 ± 0.154
3.668 ± 0.118
1.678 ± 0.068
9.759 ± 0.2
9.365 ± 0.186
9.081 ± 0.159
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.087
8.21 ± 0.173
2.882 ± 0.115
1.945 ± 0.081
3.121 ± 0.106
8.205 ± 0.193
5.245 ± 0.135
6.754 ± 0.17
0.647 ± 0.046
5.028 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here