Influenza A virus (A/ruddy turnstone/Delaware/24/1990(mixed))
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G7WV29|G7WV29_9INFA Isoform of G7WV27 Matrix protein 1 OS=Influenza A virus (A/ruddy turnstone/Delaware/24/1990(mixed)) OX=1093550 GN=M1 PE=3 SV=1
MM1 pKa = 7.38 SLLTEE6 pKa = 4.4 VEE8 pKa = 4.38 TPTRR12 pKa = 11.84 NGWEE16 pKa = 4.59 CKK18 pKa = 10.26 CSDD21 pKa = 3.73 SSDD24 pKa = 3.78 PLVIAASIIGILHH37 pKa = 7.1 LILWILDD44 pKa = 3.39 RR45 pKa = 11.84 LFFKK49 pKa = 10.78 CIYY52 pKa = 9.95 RR53 pKa = 11.84 RR54 pKa = 11.84 LKK56 pKa = 10.55 YY57 pKa = 10.03 GLKK60 pKa = 10.09 RR61 pKa = 11.84 GPSTEE66 pKa = 4.02 GVPEE70 pKa = 4.24 SMRR73 pKa = 11.84 EE74 pKa = 3.92 EE75 pKa = 4.1 YY76 pKa = 10.41 RR77 pKa = 11.84 QEE79 pKa = 3.74 QQSAVDD85 pKa = 3.3 VDD87 pKa = 4.47 DD88 pKa = 3.92 GHH90 pKa = 6.42 FVNIEE95 pKa = 3.83 LEE97 pKa = 4.08
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.904
IPC2_protein 5.003
IPC_protein 4.851
Toseland 4.736
ProMoST 4.825
Dawson 4.8
Bjellqvist 4.991
Wikipedia 4.647
Rodwell 4.711
Grimsley 4.647
Solomon 4.787
Lehninger 4.749
Nozaki 4.914
DTASelect 5.029
Thurlkill 4.736
EMBOSS 4.673
Sillero 4.978
Patrickios 3.935
IPC_peptide 4.8
IPC2_peptide 4.978
IPC2.peptide.svr19 4.965
Protein with the highest isoelectric point:
>tr|G7WV33|G7WV33_9INFA Isoform of G7WV32 Nucleoprotein OS=Influenza A virus (A/ruddy turnstone/Delaware/24/1990(mixed)) OX=1093550 GN=NP PE=3 SV=1
MM1 pKa = 7.78 ASQGTKK7 pKa = 9.71 RR8 pKa = 11.84 SYY10 pKa = 10.66 EE11 pKa = 3.79 QMEE14 pKa = 4.56 TGGEE18 pKa = 4.0 RR19 pKa = 11.84 QNATEE24 pKa = 3.76 IRR26 pKa = 11.84 ASVGRR31 pKa = 11.84 MVGGIGRR38 pKa = 11.84 FYY40 pKa = 10.72 IQMCTEE46 pKa = 4.87 LKK48 pKa = 10.71 LSDD51 pKa = 3.6 YY52 pKa = 10.66 EE53 pKa = 4.36 GRR55 pKa = 11.84 LIQNSITIEE64 pKa = 4.06 RR65 pKa = 11.84 MVLSAFDD72 pKa = 3.57 EE73 pKa = 4.31 RR74 pKa = 11.84 RR75 pKa = 11.84 NKK77 pKa = 10.12 YY78 pKa = 10.45 LEE80 pKa = 3.98 EE81 pKa = 4.54 HH82 pKa = 6.8 PSAGKK87 pKa = 10.07 DD88 pKa = 3.33 PKK90 pKa = 10.14 KK91 pKa = 9.42 TGGPIYY97 pKa = 10.21 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 DD101 pKa = 3.68 GKK103 pKa = 9.47 WMRR106 pKa = 11.84 EE107 pKa = 3.73 LILYY111 pKa = 9.97 DD112 pKa = 3.35 KK113 pKa = 11.39 EE114 pKa = 3.93 EE115 pKa = 3.69 IRR117 pKa = 11.84 RR118 pKa = 11.84 IWRR121 pKa = 11.84 QANNGEE127 pKa = 4.33 DD128 pKa = 3.23 ATAGLTHH135 pKa = 7.15 LMIWHH140 pKa = 6.74 SNLNDD145 pKa = 3.0 ATYY148 pKa = 10.71 QRR150 pKa = 11.84 TRR152 pKa = 11.84 ALVRR156 pKa = 11.84 TGMDD160 pKa = 2.98 PRR162 pKa = 11.84 MCSLMQGSTLPRR174 pKa = 11.84 RR175 pKa = 11.84 SGAAGAAVKK184 pKa = 10.56 GVGTMVMEE192 pKa = 5.61 LIRR195 pKa = 11.84 MIKK198 pKa = 10.19 RR199 pKa = 11.84 GINDD203 pKa = 3.34 RR204 pKa = 11.84 NFWRR208 pKa = 11.84 GEE210 pKa = 3.54 NGRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 IAYY219 pKa = 9.45 EE220 pKa = 3.95 RR221 pKa = 11.84 MCNILKK227 pKa = 10.75 GKK229 pKa = 9.34 FQTAAQRR236 pKa = 11.84 AMMDD240 pKa = 3.11 QVRR243 pKa = 11.84 EE244 pKa = 4.04 SRR246 pKa = 11.84 NPGNAEE252 pKa = 3.62 IEE254 pKa = 4.16 DD255 pKa = 4.8 LIFLARR261 pKa = 11.84 SALILRR267 pKa = 11.84 GSVAHH272 pKa = 6.78 KK273 pKa = 10.44 SCLPACVYY281 pKa = 10.62 GLAVASGYY289 pKa = 10.7 DD290 pKa = 3.72 FEE292 pKa = 6.06 RR293 pKa = 11.84 EE294 pKa = 4.56 GYY296 pKa = 9.53 SLVGIDD302 pKa = 5.28 PFRR305 pKa = 11.84 LLQNSQVFSLIRR317 pKa = 11.84 PNEE320 pKa = 4.06 NPAHH324 pKa = 6.86 KK325 pKa = 10.49 SQLVWMACHH334 pKa = 6.25 SAAFEE339 pKa = 4.24 DD340 pKa = 4.17 LRR342 pKa = 11.84 VSSFIRR348 pKa = 11.84 GTRR351 pKa = 11.84 VVPRR355 pKa = 11.84 GQLSTRR361 pKa = 11.84 GVQIASNEE369 pKa = 3.94 NMEE372 pKa = 4.48 TMDD375 pKa = 4.72 SSTLEE380 pKa = 3.59 LRR382 pKa = 11.84 SRR384 pKa = 11.84 YY385 pKa = 7.55 WAIRR389 pKa = 11.84 TRR391 pKa = 11.84 SGGNTNQQRR400 pKa = 11.84 ASAGQISVQPTFSVQRR416 pKa = 11.84 NLPFEE421 pKa = 4.38 RR422 pKa = 11.84 ATIMAAFTGNTEE434 pKa = 3.86 GRR436 pKa = 11.84 TSDD439 pKa = 3.19 MRR441 pKa = 11.84 TEE443 pKa = 4.9 IIRR446 pKa = 11.84 MMEE449 pKa = 3.73 NARR452 pKa = 11.84 PEE454 pKa = 4.17 DD455 pKa = 3.45 VSFQGRR461 pKa = 11.84 GVFEE465 pKa = 4.95 LSDD468 pKa = 3.67 EE469 pKa = 4.35 KK470 pKa = 10.77 ATNPIVPSFDD480 pKa = 3.22 MSNEE484 pKa = 3.24 GSYY487 pKa = 11.04 FFGDD491 pKa = 3.16 NAEE494 pKa = 4.48 EE495 pKa = 4.16 YY496 pKa = 11.1 DD497 pKa = 3.62 NN498 pKa = 4.9
Molecular weight: 56.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.887
IPC2_protein 8.77
IPC_protein 8.843
Toseland 9.253
ProMoST 9.194
Dawson 9.604
Bjellqvist 9.458
Wikipedia 9.823
Rodwell 9.692
Grimsley 9.692
Solomon 9.663
Lehninger 9.604
Nozaki 9.502
DTASelect 9.385
Thurlkill 9.443
EMBOSS 9.721
Sillero 9.604
Patrickios 5.003
IPC_peptide 9.648
IPC2_peptide 8.346
IPC2.peptide.svr19 7.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
4
11
3554
97
566
323.1
36.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.415 ± 0.639
2.251 ± 0.464
4.558 ± 0.269
6.866 ± 0.5
3.517 ± 0.225
7.569 ± 0.373
1.632 ± 0.164
6.922 ± 0.599
4.277 ± 0.276
7.653 ± 0.699
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.433 ± 0.564
5.74 ± 0.584
3.686 ± 0.3
4.052 ± 0.449
7.372 ± 1.016
8.047 ± 0.349
6.19 ± 0.351
5.627 ± 0.443
1.66 ± 0.248
2.532 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here