Turnip curly top virus
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0CSD0|L0CSD0_9GEMI Movement protein OS=Turnip curly top virus OX=859650 PE=4 SV=1
MM1 pKa = 7.42 IWRR4 pKa = 11.84 VNKK7 pKa = 10.0 DD8 pKa = 3.35 EE9 pKa = 4.01 KK10 pKa = 10.39 RR11 pKa = 11.84 VRR13 pKa = 11.84 AFRR16 pKa = 11.84 YY17 pKa = 8.4 KK18 pKa = 10.17 KK19 pKa = 9.97 RR20 pKa = 11.84 RR21 pKa = 11.84 FYY23 pKa = 11.51 SLAEE27 pKa = 3.98 YY28 pKa = 10.82 LLAPLATHH36 pKa = 6.93 LCKK39 pKa = 10.56 ARR41 pKa = 11.84 IFPDD45 pKa = 3.47 DD46 pKa = 4.04 KK47 pKa = 11.3 YY48 pKa = 11.38 LVPAYY53 pKa = 9.31 TDD55 pKa = 3.2 EE56 pKa = 5.01 AYY58 pKa = 11.07 SFLVFLKK65 pKa = 10.63 EE66 pKa = 3.78 EE67 pKa = 4.17 TRR69 pKa = 11.84 TPFQILKK76 pKa = 10.45 DD77 pKa = 3.52 EE78 pKa = 4.46 WNVEE82 pKa = 4.15 FKK84 pKa = 10.63 EE85 pKa = 4.18 IEE87 pKa = 4.16 VEE89 pKa = 4.2 LSRR92 pKa = 11.84 LRR94 pKa = 11.84 FDD96 pKa = 4.07 PKK98 pKa = 11.14 DD99 pKa = 3.77 PGEE102 pKa = 4.15 EE103 pKa = 4.15 ACSEE107 pKa = 4.11 EE108 pKa = 5.4 SPVQQSSPVQSSSCEE123 pKa = 3.92 TT124 pKa = 3.49
Molecular weight: 14.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.359
IPC2_protein 5.588
IPC_protein 5.461
Toseland 5.397
ProMoST 5.575
Dawson 5.436
Bjellqvist 5.55
Wikipedia 5.283
Rodwell 5.372
Grimsley 5.321
Solomon 5.436
Lehninger 5.397
Nozaki 5.563
DTASelect 5.664
Thurlkill 5.423
EMBOSS 5.347
Sillero 5.652
Patrickios 4.24
IPC_peptide 5.448
IPC2_peptide 5.639
IPC2.peptide.svr19 5.806
Protein with the highest isoelectric point:
>tr|L0CRA1|L0CRA1_9GEMI Replication-associated protein OS=Turnip curly top virus OX=859650 PE=3 SV=1
MM1 pKa = 7.45 SGTWSLKK8 pKa = 8.4 RR9 pKa = 11.84 SRR11 pKa = 11.84 WNSPGYY17 pKa = 10.51 DD18 pKa = 3.76 LTPKK22 pKa = 10.03 TPAKK26 pKa = 9.96 RR27 pKa = 11.84 PAVRR31 pKa = 11.84 RR32 pKa = 11.84 ALFNNQAQYY41 pKa = 11.39 SRR43 pKa = 11.84 VAARR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 WSKK52 pKa = 10.82 VSVRR56 pKa = 11.84 GNKK59 pKa = 9.29 PYY61 pKa = 10.52 RR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 LKK67 pKa = 10.76 ASDD70 pKa = 3.25 YY71 pKa = 10.87 QIYY74 pKa = 9.81 KK75 pKa = 10.77 DD76 pKa = 5.49 KK77 pKa = 11.17 IGGDD81 pKa = 3.69 NGWTVTFSGDD91 pKa = 3.06 CTMLNNYY98 pKa = 8.7 VRR100 pKa = 11.84 GIGRR104 pKa = 11.84 DD105 pKa = 3.36 QRR107 pKa = 11.84 DD108 pKa = 3.3 SVMTKK113 pKa = 7.87 TAHH116 pKa = 4.22 MHH118 pKa = 5.51 FNGVLMANDD127 pKa = 4.52 AFWEE131 pKa = 4.53 APNYY135 pKa = 6.13 MTMYY139 pKa = 10.36 SWIILDD145 pKa = 4.08 NDD147 pKa = 3.98 PGGTFPKK154 pKa = 10.28 PSDD157 pKa = 3.54 IFDD160 pKa = 3.58 MEE162 pKa = 4.48 YY163 pKa = 10.97 KK164 pKa = 9.98 EE165 pKa = 4.46 FPSMYY170 pKa = 9.81 EE171 pKa = 3.57 VAEE174 pKa = 4.3 SVKK177 pKa = 10.65 SRR179 pKa = 11.84 FIVKK183 pKa = 10.19 RR184 pKa = 11.84 KK185 pKa = 7.01 TEE187 pKa = 3.84 HH188 pKa = 5.95 YY189 pKa = 10.34 LRR191 pKa = 11.84 STGVAFGEE199 pKa = 4.24 KK200 pKa = 9.68 QNYY203 pKa = 7.41 KK204 pKa = 10.24 APNVGPIKK212 pKa = 10.58 KK213 pKa = 9.24 PIRR216 pKa = 11.84 MKK218 pKa = 10.48 FRR220 pKa = 11.84 NIWQPSEE227 pKa = 3.97 WKK229 pKa = 9.01 DD230 pKa = 3.43 TAGGKK235 pKa = 10.06 YY236 pKa = 10.11 EE237 pKa = 4.17 DD238 pKa = 4.21 LKK240 pKa = 11.25 KK241 pKa = 10.83 GALLYY246 pKa = 11.03 VCICDD251 pKa = 3.71 NKK253 pKa = 9.7 ATQFSFNLKK262 pKa = 9.68 GQWTMYY268 pKa = 10.19 FINRR272 pKa = 11.84 DD273 pKa = 3.02 LMYY276 pKa = 11.06
Molecular weight: 32.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.179
IPC2_protein 9.414
IPC_protein 9.428
Toseland 9.999
ProMoST 9.721
Dawson 10.218
Bjellqvist 9.897
Wikipedia 10.394
Rodwell 10.628
Grimsley 10.292
Solomon 10.233
Lehninger 10.204
Nozaki 9.999
DTASelect 9.897
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 10.028
IPC_peptide 10.233
IPC2_peptide 8.551
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1138
85
371
189.7
21.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.921 ± 0.675
1.406 ± 0.224
5.097 ± 0.697
5.8 ± 0.938
4.833 ± 0.685
5.448 ± 0.723
3.427 ± 0.856
7.03 ± 1.027
6.678 ± 0.843
7.557 ± 0.897
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.855
5.097 ± 0.652
5.8 ± 0.618
4.13 ± 0.426
6.415 ± 0.892
7.909 ± 1.077
5.536 ± 0.52
4.745 ± 0.576
1.845 ± 0.398
4.042 ± 0.663
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here