Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328R3F5|A0A328R3F5_9BACT Glycerol-3-phosphate acyltransferase OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04 OX=2184343 GN=plsY PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.33KK3 pKa = 10.41NKK5 pKa = 7.57TLRR8 pKa = 11.84LYY10 pKa = 9.74TSVVAAILTIFLVSCGGKK28 pKa = 8.35TSNLKK33 pKa = 10.61DD34 pKa = 3.33IADD37 pKa = 4.37DD38 pKa = 3.49THH40 pKa = 6.54EE41 pKa = 4.8PYY43 pKa = 10.66SGEE46 pKa = 3.94ATLSFAINLDD56 pKa = 3.59SDD58 pKa = 4.24LSSQIQEE65 pKa = 3.86GSAPVSDD72 pKa = 3.93PSTRR76 pKa = 11.84ISGLSLSEE84 pKa = 4.02GSSNANTYY92 pKa = 7.19TNCDD96 pKa = 3.24GQVQAFDD103 pKa = 3.41IDD105 pKa = 3.3KK106 pKa = 11.19GLFVSLAIPVSIEE119 pKa = 3.57GNNAKK124 pKa = 10.1VYY126 pKa = 9.69NVKK129 pKa = 10.43PGEE132 pKa = 4.28TYY134 pKa = 10.5SISLSCPVDD143 pKa = 3.67GEE145 pKa = 4.9KK146 pKa = 10.52SLQLQSLASVPKK158 pKa = 10.53DD159 pKa = 3.09ADD161 pKa = 3.47SSEE164 pKa = 4.7EE165 pKa = 3.92IVSDD169 pKa = 3.33ISEE172 pKa = 4.1TTAYY176 pKa = 10.68RR177 pKa = 11.84MAAFNEE183 pKa = 4.24AVDD186 pKa = 4.22DD187 pKa = 4.8LVTEE191 pKa = 4.4LEE193 pKa = 4.41KK194 pKa = 10.64IEE196 pKa = 4.87LSISQKK202 pKa = 9.01MDD204 pKa = 4.08EE205 pKa = 4.55IAALLDD211 pKa = 4.19DD212 pKa = 4.24VSSTVTTFIAEE223 pKa = 4.23QKK225 pKa = 10.52LQFPDD230 pKa = 4.25KK231 pKa = 10.14EE232 pKa = 4.57DD233 pKa = 4.11LFIDD237 pKa = 4.92PIVLLSEE244 pKa = 4.53TPDD247 pKa = 3.11SDD249 pKa = 3.72TFLDD253 pKa = 4.93KK254 pKa = 11.15VSQDD258 pKa = 3.69LLSEE262 pKa = 3.97EE263 pKa = 4.61SIITKK268 pKa = 9.84LQEE271 pKa = 4.04SSDD274 pKa = 4.09TVVSTLYY281 pKa = 10.1IDD283 pKa = 3.6SQTISKK289 pKa = 9.99DD290 pKa = 3.04QSLFLLRR297 pKa = 11.84SLLPSIQFDD306 pKa = 4.29SISNTALLDD315 pKa = 3.72VFSDD319 pKa = 4.78IIDD322 pKa = 3.63QNKK325 pKa = 9.57SVSIEE330 pKa = 4.04TLAEE334 pKa = 3.93ASTPSLYY341 pKa = 10.49SDD343 pKa = 3.4SSSFSLYY350 pKa = 10.54AISATRR356 pKa = 11.84TLLDD360 pKa = 3.13QTYY363 pKa = 7.45NTPSPIPSDD372 pKa = 3.28INEE375 pKa = 5.37DD376 pKa = 3.16ILKK379 pKa = 10.59SVFSNEE385 pKa = 5.12DD386 pKa = 3.25SQTWTARR393 pKa = 11.84SDD395 pKa = 3.67EE396 pKa = 4.34EE397 pKa = 4.46LSSLEE402 pKa = 3.72ITGAQAFIAFYY413 pKa = 9.93SATMSAQLNTSDD425 pKa = 3.85EE426 pKa = 4.34NIIAFSNKK434 pKa = 8.7LRR436 pKa = 11.84SIVFSSNTQSSAPSPGYY453 pKa = 9.04FEE455 pKa = 5.23RR456 pKa = 11.84FNFEE460 pKa = 3.52NTQYY464 pKa = 11.5SFDD467 pKa = 4.67VYY469 pKa = 11.3DD470 pKa = 3.99EE471 pKa = 5.31DD472 pKa = 5.09DD473 pKa = 4.01NEE475 pKa = 4.39VYY477 pKa = 10.05RR478 pKa = 11.84VSMKK482 pKa = 10.76SIVTQEE488 pKa = 3.73SGTTYY493 pKa = 9.87VNHH496 pKa = 6.24YY497 pKa = 10.37FLTKK501 pKa = 10.02EE502 pKa = 4.16NNTISIGKK510 pKa = 8.76VLSISAGPDD519 pKa = 3.31YY520 pKa = 10.79IATSTDD526 pKa = 2.78EE527 pKa = 4.92GEE529 pKa = 4.41FSEE532 pKa = 5.34SLPLQDD538 pKa = 5.53SSDD541 pKa = 3.62FTPFLNLYY549 pKa = 8.19RR550 pKa = 11.84TGFVSDD556 pKa = 4.36LSIDD560 pKa = 3.56GANFSKK566 pKa = 10.98ALTLINDD573 pKa = 4.07KK574 pKa = 11.09GRR576 pKa = 11.84LFYY579 pKa = 10.42MPEE582 pKa = 3.62SGIIIGFDD590 pKa = 2.95GSSNYY595 pKa = 9.5EE596 pKa = 3.94FNATSFDD603 pKa = 4.25DD604 pKa = 5.37LLSQYY609 pKa = 10.85PEE611 pKa = 4.14LQNLDD616 pKa = 3.76SLEE619 pKa = 4.39VNSHH623 pKa = 3.91QTYY626 pKa = 10.65DD627 pKa = 3.1ITDD630 pKa = 3.4NGLVLRR636 pKa = 11.84EE637 pKa = 3.94EE638 pKa = 4.4SDD640 pKa = 3.77EE641 pKa = 4.24EE642 pKa = 3.96EE643 pKa = 4.79HH644 pKa = 7.1EE645 pKa = 4.24NDD647 pKa = 3.43SAIDD651 pKa = 3.72EE652 pKa = 4.4SHH654 pKa = 6.93EE655 pKa = 4.35EE656 pKa = 4.07NLSDD660 pKa = 4.91GYY662 pKa = 10.21FEE664 pKa = 5.22RR665 pKa = 11.84FNFEE669 pKa = 3.7NAQYY673 pKa = 10.8SYY675 pKa = 9.84EE676 pKa = 4.18TYY678 pKa = 7.98TTGEE682 pKa = 4.14DD683 pKa = 3.46SQITKK688 pKa = 8.51VTVKK692 pKa = 10.69SIATEE697 pKa = 4.09EE698 pKa = 4.12AGTTYY703 pKa = 10.52FNYY706 pKa = 9.68YY707 pKa = 10.46FAMQSLNNTITIINPFRR724 pKa = 11.84FSITEE729 pKa = 3.73NSISIITDD737 pKa = 2.89EE738 pKa = 4.96GEE740 pKa = 4.22FTEE743 pKa = 5.73SLPLPNTSVLTPFLDD758 pKa = 3.53GHH760 pKa = 5.8LQGFISNLSVDD771 pKa = 3.04GTTFNKK777 pKa = 10.01ALKK780 pKa = 10.23LINPWGQSFYY790 pKa = 8.42MTEE793 pKa = 3.92SGIMIDD799 pKa = 3.76TNSSFNYY806 pKa = 9.97DD807 pKa = 3.6FNSSSFDD814 pKa = 3.7DD815 pKa = 4.66LLNEE819 pKa = 4.02YY820 pKa = 10.18PEE822 pKa = 4.35LQSFEE827 pKa = 4.35EE828 pKa = 4.39FNNPKK833 pKa = 9.94TYY835 pKa = 10.81VITDD839 pKa = 3.21NRR841 pKa = 11.84LVLLEE846 pKa = 4.45EE847 pKa = 4.58IYY849 pKa = 11.4GEE851 pKa = 3.79FDD853 pKa = 3.84SNVEE857 pKa = 4.03EE858 pKa = 4.36GHH860 pKa = 6.43EE861 pKa = 4.21EE862 pKa = 4.0DD863 pKa = 3.33AHH865 pKa = 8.36SYY867 pKa = 11.32ASDD870 pKa = 4.22LFNQPILALKK880 pKa = 9.93YY881 pKa = 10.36DD882 pKa = 3.56EE883 pKa = 5.14SGQVTHH889 pKa = 5.98EE890 pKa = 4.04VKK892 pKa = 10.55YY893 pKa = 11.06DD894 pKa = 3.3RR895 pKa = 11.84FGFPTIYY902 pKa = 10.44KK903 pKa = 9.68SVYY906 pKa = 9.03TNGQKK911 pKa = 10.18VSSSLDD917 pKa = 3.41PYY919 pKa = 10.45SISSAYY925 pKa = 9.59VAGAYY930 pKa = 9.35DD931 pKa = 3.76NKK933 pKa = 10.89SPEE936 pKa = 4.38DD937 pKa = 3.5DD938 pKa = 3.61VAINMNGQGFLTEE951 pKa = 4.31YY952 pKa = 9.52SHH954 pKa = 7.84SYY956 pKa = 11.19GNVTLKK962 pKa = 10.78YY963 pKa = 9.9SYY965 pKa = 10.88NSQGLVEE972 pKa = 4.18RR973 pKa = 11.84EE974 pKa = 3.53EE975 pKa = 4.66YY976 pKa = 10.46YY977 pKa = 11.24DD978 pKa = 3.62NGEE981 pKa = 4.1LFSIEE986 pKa = 3.57IYY988 pKa = 10.33EE989 pKa = 3.93YY990 pKa = 11.2SEE992 pKa = 3.86NLKK995 pKa = 10.45HH996 pKa = 6.53IKK998 pKa = 9.21TSSYY1002 pKa = 10.44YY1003 pKa = 9.58PEE1005 pKa = 5.21KK1006 pKa = 10.73NEE1008 pKa = 3.56QYY1010 pKa = 11.28VYY1012 pKa = 10.65IFSEE1016 pKa = 4.31DD1017 pKa = 3.65DD1018 pKa = 2.98KK1019 pKa = 11.3TATLRR1024 pKa = 11.84KK1025 pKa = 8.55STIDD1029 pKa = 3.45VPSYY1033 pKa = 9.69EE1034 pKa = 4.06YY1035 pKa = 10.66SGEE1038 pKa = 4.18VISDD1042 pKa = 3.42GTTIEE1047 pKa = 4.44TNFIMTNEE1055 pKa = 3.9SEE1057 pKa = 4.37YY1058 pKa = 10.53RR1059 pKa = 11.84RR1060 pKa = 11.84GWQEE1064 pKa = 3.73VNEE1067 pKa = 4.29EE1068 pKa = 4.04EE1069 pKa = 5.56HH1070 pKa = 7.22EE1071 pKa = 4.31NTSNGYY1077 pKa = 7.83FEE1079 pKa = 5.34RR1080 pKa = 11.84FNFEE1084 pKa = 3.49NAQYY1088 pKa = 10.77SIEE1091 pKa = 4.34TYY1093 pKa = 10.67NEE1095 pKa = 3.31EE1096 pKa = 3.97DD1097 pKa = 3.62DD1098 pKa = 4.83EE1099 pKa = 4.76YY1100 pKa = 11.44EE1101 pKa = 4.01EE1102 pKa = 4.92MIVKK1106 pKa = 8.89TIVTEE1111 pKa = 4.19EE1112 pKa = 4.05AGTTYY1117 pKa = 11.48ANIYY1121 pKa = 9.02FIQEE1125 pKa = 3.96EE1126 pKa = 4.57SSSITIGKK1134 pKa = 9.01FLSLAITQDD1143 pKa = 3.76SISNITDD1150 pKa = 3.46DD1151 pKa = 5.45GEE1153 pKa = 4.5FTTSFPLTNSNEE1165 pKa = 3.91RR1166 pKa = 11.84TPFLNYY1172 pKa = 10.18EE1173 pKa = 4.31SVGFAKK1179 pKa = 10.7DD1180 pKa = 2.93IDD1182 pKa = 4.13FDD1184 pKa = 4.01GVTISKK1190 pKa = 9.87ALKK1193 pKa = 10.59VIDD1196 pKa = 4.74KK1197 pKa = 11.12GNVDD1201 pKa = 3.96DD1202 pKa = 5.7GEE1204 pKa = 4.2EE1205 pKa = 4.04EE1206 pKa = 4.15EE1207 pKa = 4.53IEE1209 pKa = 4.58FIISEE1214 pKa = 4.15SGKK1217 pKa = 9.82ILSSEE1222 pKa = 3.66WTNIQFNSSGFDD1234 pKa = 3.25EE1235 pKa = 5.67LLNQYY1240 pKa = 10.39PEE1242 pKa = 4.17LQNFEE1247 pKa = 4.25NLEE1250 pKa = 4.32AGNPEE1255 pKa = 4.42YY1256 pKa = 11.34YY1257 pKa = 10.37EE1258 pKa = 3.84ITDD1261 pKa = 3.38NGLVLEE1267 pKa = 4.45EE1268 pKa = 5.68DD1269 pKa = 3.5EE1270 pKa = 6.68DD1271 pKa = 4.38EE1272 pKa = 5.74DD1273 pKa = 5.63EE1274 pKa = 4.5EE1275 pKa = 5.93SNDD1278 pKa = 3.37NGSNQDD1284 pKa = 3.05SDD1286 pKa = 4.66EE1287 pKa = 4.07NTEE1290 pKa = 4.46DD1291 pKa = 3.45SSSANTIGFEE1301 pKa = 4.04YY1302 pKa = 10.75SHH1304 pKa = 7.51DD1305 pKa = 3.43KK1306 pKa = 8.94TVYY1309 pKa = 9.84YY1310 pKa = 10.61EE1311 pKa = 4.92DD1312 pKa = 3.79IEE1314 pKa = 5.01SIQFTKK1320 pKa = 10.72VVDD1323 pKa = 4.02GEE1325 pKa = 4.31IGGEE1329 pKa = 4.03LLLDD1333 pKa = 3.86ALEE1336 pKa = 4.18TSKK1339 pKa = 11.42AFLLKK1344 pKa = 10.54ALSPDD1349 pKa = 3.06SSITIEE1355 pKa = 4.44EE1356 pKa = 3.45IDD1358 pKa = 3.89AYY1360 pKa = 10.31FVYY1363 pKa = 10.3LKK1365 pKa = 10.0EE1366 pKa = 4.11YY1367 pKa = 10.1EE1368 pKa = 3.86ISFTGKK1374 pKa = 9.19MSGPKK1379 pKa = 9.79GFMEE1383 pKa = 5.5FGHH1386 pKa = 6.85PYY1388 pKa = 7.84WGIRR1392 pKa = 11.84AVYY1395 pKa = 7.37EE1396 pKa = 4.24TLSEE1400 pKa = 3.99ALEE1403 pKa = 4.16SQEE1406 pKa = 4.92FDD1408 pKa = 3.46VTVQAMLYY1416 pKa = 8.62GVSGGTEE1423 pKa = 4.1TVASGMARR1431 pKa = 11.84IAYY1434 pKa = 10.25SNILYY1439 pKa = 9.64GLKK1442 pKa = 10.3YY1443 pKa = 10.39GYY1445 pKa = 10.21SQDD1448 pKa = 3.45TDD1450 pKa = 3.21GDD1452 pKa = 4.62SIVDD1456 pKa = 3.76NQDD1459 pKa = 2.88AFPNNAEE1466 pKa = 3.91EE1467 pKa = 4.41WADD1470 pKa = 3.45TDD1472 pKa = 5.16LDD1474 pKa = 4.8GIGDD1478 pKa = 3.91NSDD1481 pKa = 3.26TSPYY1485 pKa = 9.64QHH1487 pKa = 6.96SDD1489 pKa = 3.38YY1490 pKa = 11.41QEE1492 pKa = 3.78IALGSDD1498 pKa = 2.97EE1499 pKa = 6.44DD1500 pKa = 3.8IFKK1503 pKa = 11.02SLEE1506 pKa = 3.95NNTFNSSDD1514 pKa = 3.3SSTGEE1519 pKa = 3.47ISLEE1523 pKa = 3.69ALLASEE1529 pKa = 5.19KK1530 pKa = 10.48FLEE1533 pKa = 4.33LFGDD1537 pKa = 3.77EE1538 pKa = 4.43SVDD1541 pKa = 3.65YY1542 pKa = 10.43TSLDD1546 pKa = 3.19ISKK1549 pKa = 10.2HH1550 pKa = 5.71LEE1552 pKa = 4.0LLSKK1556 pKa = 9.85TEE1558 pKa = 4.89LYY1560 pKa = 8.27FTYY1563 pKa = 10.21LKK1565 pKa = 10.82SSLEE1569 pKa = 3.91FHH1571 pKa = 6.91SGNGFDD1577 pKa = 4.94SKK1579 pKa = 11.25APKK1582 pKa = 10.49DD1583 pKa = 4.27FIKK1586 pKa = 10.55SGYY1589 pKa = 8.0EE1590 pKa = 3.36WSEE1593 pKa = 3.78VNIYY1597 pKa = 10.87SSVSEE1602 pKa = 4.23AFGSQDD1608 pKa = 2.91SGIVIQHH1615 pKa = 6.47ILDD1618 pKa = 4.5GYY1620 pKa = 7.79TFQTSLVSDD1629 pKa = 4.7GLLEE1633 pKa = 3.85MAKK1636 pKa = 10.7NIVEE1640 pKa = 4.11YY1641 pKa = 10.42GKK1643 pKa = 10.63EE1644 pKa = 3.89FGYY1647 pKa = 7.73TTATPP1652 pKa = 3.6

Molecular weight:
184.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328R373|A0A328R373_9BACT Uncharacterized protein (Fragment) OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04 OX=2184343 GN=DID78_07105 PE=4 SV=1
MM1 pKa = 6.88VAPVAAKK8 pKa = 10.31VIADD12 pKa = 4.13RR13 pKa = 11.84NSRR16 pKa = 11.84GAPNQNRR23 pKa = 11.84SGFSFQGSTSPFQRR37 pKa = 11.84PLSQWQTVGNIGNNVSIEE55 pKa = 3.95YY56 pKa = 10.6APTNEE61 pKa = 3.74LPVRR65 pKa = 11.84NSRR68 pKa = 11.84PSTPPPRR75 pKa = 11.84VPDD78 pKa = 3.84GASIIDD84 pKa = 3.51NN85 pKa = 3.88

Molecular weight:
9.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1416

0

1416

483552

29

3958

341.5

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.483 ± 0.069

1.378 ± 0.028

5.577 ± 0.058

6.384 ± 0.077

5.069 ± 0.062

5.864 ± 0.074

2.468 ± 0.046

7.921 ± 0.085

7.711 ± 0.078

9.897 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.028

4.852 ± 0.057

3.681 ± 0.046

3.728 ± 0.045

3.672 ± 0.047

8.456 ± 0.073

5.491 ± 0.059

5.949 ± 0.07

0.81 ± 0.019

3.532 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski