Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1416 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328R3F5|A0A328R3F5_9BACT Glycerol-3-phosphate acyltransferase OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04 OX=2184343 GN=plsY PE=3 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.33 KK3 pKa = 10.41 NKK5 pKa = 7.57 TLRR8 pKa = 11.84 LYY10 pKa = 9.74 TSVVAAILTIFLVSCGGKK28 pKa = 8.35 TSNLKK33 pKa = 10.61 DD34 pKa = 3.33 IADD37 pKa = 4.37 DD38 pKa = 3.49 THH40 pKa = 6.54 EE41 pKa = 4.8 PYY43 pKa = 10.66 SGEE46 pKa = 3.94 ATLSFAINLDD56 pKa = 3.59 SDD58 pKa = 4.24 LSSQIQEE65 pKa = 3.86 GSAPVSDD72 pKa = 3.93 PSTRR76 pKa = 11.84 ISGLSLSEE84 pKa = 4.02 GSSNANTYY92 pKa = 7.19 TNCDD96 pKa = 3.24 GQVQAFDD103 pKa = 3.41 IDD105 pKa = 3.3 KK106 pKa = 11.19 GLFVSLAIPVSIEE119 pKa = 3.57 GNNAKK124 pKa = 10.1 VYY126 pKa = 9.69 NVKK129 pKa = 10.43 PGEE132 pKa = 4.28 TYY134 pKa = 10.5 SISLSCPVDD143 pKa = 3.67 GEE145 pKa = 4.9 KK146 pKa = 10.52 SLQLQSLASVPKK158 pKa = 10.53 DD159 pKa = 3.09 ADD161 pKa = 3.47 SSEE164 pKa = 4.7 EE165 pKa = 3.92 IVSDD169 pKa = 3.33 ISEE172 pKa = 4.1 TTAYY176 pKa = 10.68 RR177 pKa = 11.84 MAAFNEE183 pKa = 4.24 AVDD186 pKa = 4.22 DD187 pKa = 4.8 LVTEE191 pKa = 4.4 LEE193 pKa = 4.41 KK194 pKa = 10.64 IEE196 pKa = 4.87 LSISQKK202 pKa = 9.01 MDD204 pKa = 4.08 EE205 pKa = 4.55 IAALLDD211 pKa = 4.19 DD212 pKa = 4.24 VSSTVTTFIAEE223 pKa = 4.23 QKK225 pKa = 10.52 LQFPDD230 pKa = 4.25 KK231 pKa = 10.14 EE232 pKa = 4.57 DD233 pKa = 4.11 LFIDD237 pKa = 4.92 PIVLLSEE244 pKa = 4.53 TPDD247 pKa = 3.11 SDD249 pKa = 3.72 TFLDD253 pKa = 4.93 KK254 pKa = 11.15 VSQDD258 pKa = 3.69 LLSEE262 pKa = 3.97 EE263 pKa = 4.61 SIITKK268 pKa = 9.84 LQEE271 pKa = 4.04 SSDD274 pKa = 4.09 TVVSTLYY281 pKa = 10.1 IDD283 pKa = 3.6 SQTISKK289 pKa = 9.99 DD290 pKa = 3.04 QSLFLLRR297 pKa = 11.84 SLLPSIQFDD306 pKa = 4.29 SISNTALLDD315 pKa = 3.72 VFSDD319 pKa = 4.78 IIDD322 pKa = 3.63 QNKK325 pKa = 9.57 SVSIEE330 pKa = 4.04 TLAEE334 pKa = 3.93 ASTPSLYY341 pKa = 10.49 SDD343 pKa = 3.4 SSSFSLYY350 pKa = 10.54 AISATRR356 pKa = 11.84 TLLDD360 pKa = 3.13 QTYY363 pKa = 7.45 NTPSPIPSDD372 pKa = 3.28 INEE375 pKa = 5.37 DD376 pKa = 3.16 ILKK379 pKa = 10.59 SVFSNEE385 pKa = 5.12 DD386 pKa = 3.25 SQTWTARR393 pKa = 11.84 SDD395 pKa = 3.67 EE396 pKa = 4.34 EE397 pKa = 4.46 LSSLEE402 pKa = 3.72 ITGAQAFIAFYY413 pKa = 9.93 SATMSAQLNTSDD425 pKa = 3.85 EE426 pKa = 4.34 NIIAFSNKK434 pKa = 8.7 LRR436 pKa = 11.84 SIVFSSNTQSSAPSPGYY453 pKa = 9.04 FEE455 pKa = 5.23 RR456 pKa = 11.84 FNFEE460 pKa = 3.52 NTQYY464 pKa = 11.5 SFDD467 pKa = 4.67 VYY469 pKa = 11.3 DD470 pKa = 3.99 EE471 pKa = 5.31 DD472 pKa = 5.09 DD473 pKa = 4.01 NEE475 pKa = 4.39 VYY477 pKa = 10.05 RR478 pKa = 11.84 VSMKK482 pKa = 10.76 SIVTQEE488 pKa = 3.73 SGTTYY493 pKa = 9.87 VNHH496 pKa = 6.24 YY497 pKa = 10.37 FLTKK501 pKa = 10.02 EE502 pKa = 4.16 NNTISIGKK510 pKa = 8.76 VLSISAGPDD519 pKa = 3.31 YY520 pKa = 10.79 IATSTDD526 pKa = 2.78 EE527 pKa = 4.92 GEE529 pKa = 4.41 FSEE532 pKa = 5.34 SLPLQDD538 pKa = 5.53 SSDD541 pKa = 3.62 FTPFLNLYY549 pKa = 8.19 RR550 pKa = 11.84 TGFVSDD556 pKa = 4.36 LSIDD560 pKa = 3.56 GANFSKK566 pKa = 10.98 ALTLINDD573 pKa = 4.07 KK574 pKa = 11.09 GRR576 pKa = 11.84 LFYY579 pKa = 10.42 MPEE582 pKa = 3.62 SGIIIGFDD590 pKa = 2.95 GSSNYY595 pKa = 9.5 EE596 pKa = 3.94 FNATSFDD603 pKa = 4.25 DD604 pKa = 5.37 LLSQYY609 pKa = 10.85 PEE611 pKa = 4.14 LQNLDD616 pKa = 3.76 SLEE619 pKa = 4.39 VNSHH623 pKa = 3.91 QTYY626 pKa = 10.65 DD627 pKa = 3.1 ITDD630 pKa = 3.4 NGLVLRR636 pKa = 11.84 EE637 pKa = 3.94 EE638 pKa = 4.4 SDD640 pKa = 3.77 EE641 pKa = 4.24 EE642 pKa = 3.96 EE643 pKa = 4.79 HH644 pKa = 7.1 EE645 pKa = 4.24 NDD647 pKa = 3.43 SAIDD651 pKa = 3.72 EE652 pKa = 4.4 SHH654 pKa = 6.93 EE655 pKa = 4.35 EE656 pKa = 4.07 NLSDD660 pKa = 4.91 GYY662 pKa = 10.21 FEE664 pKa = 5.22 RR665 pKa = 11.84 FNFEE669 pKa = 3.7 NAQYY673 pKa = 10.8 SYY675 pKa = 9.84 EE676 pKa = 4.18 TYY678 pKa = 7.98 TTGEE682 pKa = 4.14 DD683 pKa = 3.46 SQITKK688 pKa = 8.51 VTVKK692 pKa = 10.69 SIATEE697 pKa = 4.09 EE698 pKa = 4.12 AGTTYY703 pKa = 10.52 FNYY706 pKa = 9.68 YY707 pKa = 10.46 FAMQSLNNTITIINPFRR724 pKa = 11.84 FSITEE729 pKa = 3.73 NSISIITDD737 pKa = 2.89 EE738 pKa = 4.96 GEE740 pKa = 4.22 FTEE743 pKa = 5.73 SLPLPNTSVLTPFLDD758 pKa = 3.53 GHH760 pKa = 5.8 LQGFISNLSVDD771 pKa = 3.04 GTTFNKK777 pKa = 10.01 ALKK780 pKa = 10.23 LINPWGQSFYY790 pKa = 8.42 MTEE793 pKa = 3.92 SGIMIDD799 pKa = 3.76 TNSSFNYY806 pKa = 9.97 DD807 pKa = 3.6 FNSSSFDD814 pKa = 3.7 DD815 pKa = 4.66 LLNEE819 pKa = 4.02 YY820 pKa = 10.18 PEE822 pKa = 4.35 LQSFEE827 pKa = 4.35 EE828 pKa = 4.39 FNNPKK833 pKa = 9.94 TYY835 pKa = 10.81 VITDD839 pKa = 3.21 NRR841 pKa = 11.84 LVLLEE846 pKa = 4.45 EE847 pKa = 4.58 IYY849 pKa = 11.4 GEE851 pKa = 3.79 FDD853 pKa = 3.84 SNVEE857 pKa = 4.03 EE858 pKa = 4.36 GHH860 pKa = 6.43 EE861 pKa = 4.21 EE862 pKa = 4.0 DD863 pKa = 3.33 AHH865 pKa = 8.36 SYY867 pKa = 11.32 ASDD870 pKa = 4.22 LFNQPILALKK880 pKa = 9.93 YY881 pKa = 10.36 DD882 pKa = 3.56 EE883 pKa = 5.14 SGQVTHH889 pKa = 5.98 EE890 pKa = 4.04 VKK892 pKa = 10.55 YY893 pKa = 11.06 DD894 pKa = 3.3 RR895 pKa = 11.84 FGFPTIYY902 pKa = 10.44 KK903 pKa = 9.68 SVYY906 pKa = 9.03 TNGQKK911 pKa = 10.18 VSSSLDD917 pKa = 3.41 PYY919 pKa = 10.45 SISSAYY925 pKa = 9.59 VAGAYY930 pKa = 9.35 DD931 pKa = 3.76 NKK933 pKa = 10.89 SPEE936 pKa = 4.38 DD937 pKa = 3.5 DD938 pKa = 3.61 VAINMNGQGFLTEE951 pKa = 4.31 YY952 pKa = 9.52 SHH954 pKa = 7.84 SYY956 pKa = 11.19 GNVTLKK962 pKa = 10.78 YY963 pKa = 9.9 SYY965 pKa = 10.88 NSQGLVEE972 pKa = 4.18 RR973 pKa = 11.84 EE974 pKa = 3.53 EE975 pKa = 4.66 YY976 pKa = 10.46 YY977 pKa = 11.24 DD978 pKa = 3.62 NGEE981 pKa = 4.1 LFSIEE986 pKa = 3.57 IYY988 pKa = 10.33 EE989 pKa = 3.93 YY990 pKa = 11.2 SEE992 pKa = 3.86 NLKK995 pKa = 10.45 HH996 pKa = 6.53 IKK998 pKa = 9.21 TSSYY1002 pKa = 10.44 YY1003 pKa = 9.58 PEE1005 pKa = 5.21 KK1006 pKa = 10.73 NEE1008 pKa = 3.56 QYY1010 pKa = 11.28 VYY1012 pKa = 10.65 IFSEE1016 pKa = 4.31 DD1017 pKa = 3.65 DD1018 pKa = 2.98 KK1019 pKa = 11.3 TATLRR1024 pKa = 11.84 KK1025 pKa = 8.55 STIDD1029 pKa = 3.45 VPSYY1033 pKa = 9.69 EE1034 pKa = 4.06 YY1035 pKa = 10.66 SGEE1038 pKa = 4.18 VISDD1042 pKa = 3.42 GTTIEE1047 pKa = 4.44 TNFIMTNEE1055 pKa = 3.9 SEE1057 pKa = 4.37 YY1058 pKa = 10.53 RR1059 pKa = 11.84 RR1060 pKa = 11.84 GWQEE1064 pKa = 3.73 VNEE1067 pKa = 4.29 EE1068 pKa = 4.04 EE1069 pKa = 5.56 HH1070 pKa = 7.22 EE1071 pKa = 4.31 NTSNGYY1077 pKa = 7.83 FEE1079 pKa = 5.34 RR1080 pKa = 11.84 FNFEE1084 pKa = 3.49 NAQYY1088 pKa = 10.77 SIEE1091 pKa = 4.34 TYY1093 pKa = 10.67 NEE1095 pKa = 3.31 EE1096 pKa = 3.97 DD1097 pKa = 3.62 DD1098 pKa = 4.83 EE1099 pKa = 4.76 YY1100 pKa = 11.44 EE1101 pKa = 4.01 EE1102 pKa = 4.92 MIVKK1106 pKa = 8.89 TIVTEE1111 pKa = 4.19 EE1112 pKa = 4.05 AGTTYY1117 pKa = 11.48 ANIYY1121 pKa = 9.02 FIQEE1125 pKa = 3.96 EE1126 pKa = 4.57 SSSITIGKK1134 pKa = 9.01 FLSLAITQDD1143 pKa = 3.76 SISNITDD1150 pKa = 3.46 DD1151 pKa = 5.45 GEE1153 pKa = 4.5 FTTSFPLTNSNEE1165 pKa = 3.91 RR1166 pKa = 11.84 TPFLNYY1172 pKa = 10.18 EE1173 pKa = 4.31 SVGFAKK1179 pKa = 10.7 DD1180 pKa = 2.93 IDD1182 pKa = 4.13 FDD1184 pKa = 4.01 GVTISKK1190 pKa = 9.87 ALKK1193 pKa = 10.59 VIDD1196 pKa = 4.74 KK1197 pKa = 11.12 GNVDD1201 pKa = 3.96 DD1202 pKa = 5.7 GEE1204 pKa = 4.2 EE1205 pKa = 4.04 EE1206 pKa = 4.15 EE1207 pKa = 4.53 IEE1209 pKa = 4.58 FIISEE1214 pKa = 4.15 SGKK1217 pKa = 9.82 ILSSEE1222 pKa = 3.66 WTNIQFNSSGFDD1234 pKa = 3.25 EE1235 pKa = 5.67 LLNQYY1240 pKa = 10.39 PEE1242 pKa = 4.17 LQNFEE1247 pKa = 4.25 NLEE1250 pKa = 4.32 AGNPEE1255 pKa = 4.42 YY1256 pKa = 11.34 YY1257 pKa = 10.37 EE1258 pKa = 3.84 ITDD1261 pKa = 3.38 NGLVLEE1267 pKa = 4.45 EE1268 pKa = 5.68 DD1269 pKa = 3.5 EE1270 pKa = 6.68 DD1271 pKa = 4.38 EE1272 pKa = 5.74 DD1273 pKa = 5.63 EE1274 pKa = 4.5 EE1275 pKa = 5.93 SNDD1278 pKa = 3.37 NGSNQDD1284 pKa = 3.05 SDD1286 pKa = 4.66 EE1287 pKa = 4.07 NTEE1290 pKa = 4.46 DD1291 pKa = 3.45 SSSANTIGFEE1301 pKa = 4.04 YY1302 pKa = 10.75 SHH1304 pKa = 7.51 DD1305 pKa = 3.43 KK1306 pKa = 8.94 TVYY1309 pKa = 9.84 YY1310 pKa = 10.61 EE1311 pKa = 4.92 DD1312 pKa = 3.79 IEE1314 pKa = 5.01 SIQFTKK1320 pKa = 10.72 VVDD1323 pKa = 4.02 GEE1325 pKa = 4.31 IGGEE1329 pKa = 4.03 LLLDD1333 pKa = 3.86 ALEE1336 pKa = 4.18 TSKK1339 pKa = 11.42 AFLLKK1344 pKa = 10.54 ALSPDD1349 pKa = 3.06 SSITIEE1355 pKa = 4.44 EE1356 pKa = 3.45 IDD1358 pKa = 3.89 AYY1360 pKa = 10.31 FVYY1363 pKa = 10.3 LKK1365 pKa = 10.0 EE1366 pKa = 4.11 YY1367 pKa = 10.1 EE1368 pKa = 3.86 ISFTGKK1374 pKa = 9.19 MSGPKK1379 pKa = 9.79 GFMEE1383 pKa = 5.5 FGHH1386 pKa = 6.85 PYY1388 pKa = 7.84 WGIRR1392 pKa = 11.84 AVYY1395 pKa = 7.37 EE1396 pKa = 4.24 TLSEE1400 pKa = 3.99 ALEE1403 pKa = 4.16 SQEE1406 pKa = 4.92 FDD1408 pKa = 3.46 VTVQAMLYY1416 pKa = 8.62 GVSGGTEE1423 pKa = 4.1 TVASGMARR1431 pKa = 11.84 IAYY1434 pKa = 10.25 SNILYY1439 pKa = 9.64 GLKK1442 pKa = 10.3 YY1443 pKa = 10.39 GYY1445 pKa = 10.21 SQDD1448 pKa = 3.45 TDD1450 pKa = 3.21 GDD1452 pKa = 4.62 SIVDD1456 pKa = 3.76 NQDD1459 pKa = 2.88 AFPNNAEE1466 pKa = 3.91 EE1467 pKa = 4.41 WADD1470 pKa = 3.45 TDD1472 pKa = 5.16 LDD1474 pKa = 4.8 GIGDD1478 pKa = 3.91 NSDD1481 pKa = 3.26 TSPYY1485 pKa = 9.64 QHH1487 pKa = 6.96 SDD1489 pKa = 3.38 YY1490 pKa = 11.41 QEE1492 pKa = 3.78 IALGSDD1498 pKa = 2.97 EE1499 pKa = 6.44 DD1500 pKa = 3.8 IFKK1503 pKa = 11.02 SLEE1506 pKa = 3.95 NNTFNSSDD1514 pKa = 3.3 SSTGEE1519 pKa = 3.47 ISLEE1523 pKa = 3.69 ALLASEE1529 pKa = 5.19 KK1530 pKa = 10.48 FLEE1533 pKa = 4.33 LFGDD1537 pKa = 3.77 EE1538 pKa = 4.43 SVDD1541 pKa = 3.65 YY1542 pKa = 10.43 TSLDD1546 pKa = 3.19 ISKK1549 pKa = 10.2 HH1550 pKa = 5.71 LEE1552 pKa = 4.0 LLSKK1556 pKa = 9.85 TEE1558 pKa = 4.89 LYY1560 pKa = 8.27 FTYY1563 pKa = 10.21 LKK1565 pKa = 10.82 SSLEE1569 pKa = 3.91 FHH1571 pKa = 6.91 SGNGFDD1577 pKa = 4.94 SKK1579 pKa = 11.25 APKK1582 pKa = 10.49 DD1583 pKa = 4.27 FIKK1586 pKa = 10.55 SGYY1589 pKa = 8.0 EE1590 pKa = 3.36 WSEE1593 pKa = 3.78 VNIYY1597 pKa = 10.87 SSVSEE1602 pKa = 4.23 AFGSQDD1608 pKa = 2.91 SGIVIQHH1615 pKa = 6.47 ILDD1618 pKa = 4.5 GYY1620 pKa = 7.79 TFQTSLVSDD1629 pKa = 4.7 GLLEE1633 pKa = 3.85 MAKK1636 pKa = 10.7 NIVEE1640 pKa = 4.11 YY1641 pKa = 10.42 GKK1643 pKa = 10.63 EE1644 pKa = 3.89 FGYY1647 pKa = 7.73 TTATPP1652 pKa = 3.6
Molecular weight: 184.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.706
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.605
Solomon 3.859
Lehninger 3.821
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.719
EMBOSS 3.757
Sillero 4.012
Patrickios 1.481
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A328R373|A0A328R373_9BACT Uncharacterized protein (Fragment) OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-D04 OX=2184343 GN=DID78_07105 PE=4 SV=1
MM1 pKa = 6.88 VAPVAAKK8 pKa = 10.31 VIADD12 pKa = 4.13 RR13 pKa = 11.84 NSRR16 pKa = 11.84 GAPNQNRR23 pKa = 11.84 SGFSFQGSTSPFQRR37 pKa = 11.84 PLSQWQTVGNIGNNVSIEE55 pKa = 3.95 YY56 pKa = 10.6 APTNEE61 pKa = 3.74 LPVRR65 pKa = 11.84 NSRR68 pKa = 11.84 PSTPPPRR75 pKa = 11.84 VPDD78 pKa = 3.84 GASIIDD84 pKa = 3.51 NN85 pKa = 3.88
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.589
IPC_protein 10.789
Toseland 10.774
ProMoST 10.877
Dawson 10.847
Bjellqvist 10.701
Wikipedia 11.184
Rodwell 10.73
Grimsley 10.906
Solomon 11.14
Lehninger 11.082
Nozaki 10.745
DTASelect 10.701
Thurlkill 10.774
EMBOSS 11.213
Sillero 10.804
Patrickios 10.657
IPC_peptide 11.14
IPC2_peptide 9.911
IPC2.peptide.svr19 8.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1416
0
1416
483552
29
3958
341.5
38.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.483 ± 0.069
1.378 ± 0.028
5.577 ± 0.058
6.384 ± 0.077
5.069 ± 0.062
5.864 ± 0.074
2.468 ± 0.046
7.921 ± 0.085
7.711 ± 0.078
9.897 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.078 ± 0.028
4.852 ± 0.057
3.681 ± 0.046
3.728 ± 0.045
3.672 ± 0.047
8.456 ± 0.073
5.491 ± 0.059
5.949 ± 0.07
0.81 ± 0.019
3.532 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here