Enterobacteria phage VT1-Sakai

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9EYA9|Q9EYA9_BPVT1 Superinfection exclusion protein B OS=Enterobacteria phage VT1-Sakai OX=462299 GN=sieB PE=4 SV=1
MM1 pKa = 7.47SNIKK5 pKa = 10.19KK6 pKa = 10.63YY7 pKa = 10.7IIDD10 pKa = 4.21YY11 pKa = 9.73DD12 pKa = 3.52WKK14 pKa = 10.79ASIEE18 pKa = 3.99IEE20 pKa = 3.8IDD22 pKa = 3.1HH23 pKa = 7.35DD24 pKa = 4.32VMTEE28 pKa = 3.99EE29 pKa = 5.57KK30 pKa = 10.35LHH32 pKa = 6.08QINNFWSDD40 pKa = 2.68SEE42 pKa = 4.22YY43 pKa = 11.07RR44 pKa = 11.84LNKK47 pKa = 9.52HH48 pKa = 5.94GSVLNAVLIMLAQHH62 pKa = 6.9ALLIAISSDD71 pKa = 2.92LNAYY75 pKa = 9.62GVVCEE80 pKa = 4.87FDD82 pKa = 3.48WNDD85 pKa = 3.26GNGQEE90 pKa = 5.08GWPSMDD96 pKa = 2.65GSEE99 pKa = 4.97GIRR102 pKa = 11.84ITDD105 pKa = 3.36IDD107 pKa = 3.67TSGIFDD113 pKa = 4.38SDD115 pKa = 3.93DD116 pKa = 3.52MTIKK120 pKa = 10.65AAA122 pKa = 3.9

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9EYC1|Q9EYC1_BPVT1 Uncharacterized protein H0042 OS=Enterobacteria phage VT1-Sakai OX=462299 GN=H0042 PE=4 SV=1
MM1 pKa = 6.97STIAEE6 pKa = 4.05LVRR9 pKa = 11.84ANFRR13 pKa = 11.84EE14 pKa = 4.1EE15 pKa = 3.58LVRR18 pKa = 11.84WYY20 pKa = 10.29RR21 pKa = 11.84YY22 pKa = 9.46RR23 pKa = 11.84SSSSLPLDD31 pKa = 3.41EE32 pKa = 6.07LYY34 pKa = 10.73EE35 pKa = 4.16HH36 pKa = 6.91SPAARR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 8.71PRR45 pKa = 11.84DD46 pKa = 3.04RR47 pKa = 11.84VLRR50 pKa = 11.84RR51 pKa = 11.84LFKK54 pKa = 11.01LNNEE58 pKa = 4.2FQRR61 pKa = 11.84NRR63 pKa = 11.84IIRR66 pKa = 11.84SLDD69 pKa = 3.3FKK71 pKa = 11.5

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

1

77

16249

25

881

211.0

23.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.939 ± 0.413

1.465 ± 0.147

5.52 ± 0.208

6.493 ± 0.282

3.157 ± 0.138

7.244 ± 0.342

1.803 ± 0.134

5.243 ± 0.247

5.25 ± 0.325

8.45 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.185

3.963 ± 0.187

4.043 ± 0.267

4.259 ± 0.251

6.948 ± 0.353

6.437 ± 0.219

5.73 ± 0.26

6.462 ± 0.241

2.0 ± 0.157

2.763 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski