Amino acid dipepetide frequency for Enterobacteria phage VT1-Sakai

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.078AlaAla: 11.078 ± 1.211
0.985AlaCys: 0.985 ± 0.271
5.539AlaAsp: 5.539 ± 0.573
8.309AlaGlu: 8.309 ± 0.974
3.385AlaPhe: 3.385 ± 0.56
8.801AlaGly: 8.801 ± 1.125
1.046AlaHis: 1.046 ± 0.247
4.924AlaIle: 4.924 ± 0.56
4.0AlaLys: 4.0 ± 0.639
8.309AlaLeu: 8.309 ± 0.758
2.954AlaMet: 2.954 ± 0.373
3.139AlaAsn: 3.139 ± 0.346
2.646AlaPro: 2.646 ± 0.501
4.0AlaGln: 4.0 ± 0.488
6.462AlaArg: 6.462 ± 0.712
7.078AlaSer: 7.078 ± 0.641
5.539AlaThr: 5.539 ± 0.707
6.524AlaVal: 6.524 ± 0.524
2.031AlaTrp: 2.031 ± 0.399
2.646AlaTyr: 2.646 ± 0.426
0.0AlaXaa: 0.0 ± 0.0
Cys
1.292CysAla: 1.292 ± 0.289
0.492CysCys: 0.492 ± 0.163
0.8CysAsp: 0.8 ± 0.205
0.677CysGlu: 0.677 ± 0.216
0.431CysPhe: 0.431 ± 0.167
1.169CysGly: 1.169 ± 0.295
0.308CysHis: 0.308 ± 0.141
0.739CysIle: 0.739 ± 0.278
0.554CysLys: 0.554 ± 0.207
0.862CysLeu: 0.862 ± 0.192
0.369CysMet: 0.369 ± 0.158
0.308CysAsn: 0.308 ± 0.139
0.554CysPro: 0.554 ± 0.203
0.369CysGln: 0.369 ± 0.142
1.6CysArg: 1.6 ± 0.358
1.231CysSer: 1.231 ± 0.289
0.862CysThr: 0.862 ± 0.2
1.169CysVal: 1.169 ± 0.243
0.369CysTrp: 0.369 ± 0.158
0.492CysTyr: 0.492 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
5.355AspAla: 5.355 ± 0.531
0.923AspCys: 0.923 ± 0.235
3.385AspAsp: 3.385 ± 0.57
4.185AspGlu: 4.185 ± 0.629
1.6AspPhe: 1.6 ± 0.295
5.108AspGly: 5.108 ± 0.572
0.739AspHis: 0.739 ± 0.205
3.323AspIle: 3.323 ± 0.448
3.139AspLys: 3.139 ± 0.452
3.693AspLeu: 3.693 ± 0.506
2.154AspMet: 2.154 ± 0.347
2.462AspAsn: 2.462 ± 0.408
2.523AspPro: 2.523 ± 0.326
1.046AspGln: 1.046 ± 0.258
3.2AspArg: 3.2 ± 0.435
2.77AspSer: 2.77 ± 0.457
3.016AspThr: 3.016 ± 0.429
3.816AspVal: 3.816 ± 0.502
1.354AspTrp: 1.354 ± 0.337
1.416AspTyr: 1.416 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
6.462GluAla: 6.462 ± 0.581
1.354GluCys: 1.354 ± 0.315
2.646GluAsp: 2.646 ± 0.396
4.0GluGlu: 4.0 ± 0.682
1.908GluPhe: 1.908 ± 0.321
4.37GluGly: 4.37 ± 0.473
1.169GluHis: 1.169 ± 0.287
4.185GluIle: 4.185 ± 0.486
4.124GluLys: 4.124 ± 0.594
5.724GluLeu: 5.724 ± 0.733
1.477GluMet: 1.477 ± 0.318
3.262GluAsn: 3.262 ± 0.344
2.216GluPro: 2.216 ± 0.371
3.447GluGln: 3.447 ± 0.628
5.047GluArg: 5.047 ± 0.668
4.247GluSer: 4.247 ± 0.571
3.077GluThr: 3.077 ± 0.429
3.262GluVal: 3.262 ± 0.457
1.046GluTrp: 1.046 ± 0.253
1.908GluTyr: 1.908 ± 0.255
0.0GluXaa: 0.0 ± 0.0
Phe
2.154PheAla: 2.154 ± 0.396
0.862PheCys: 0.862 ± 0.22
1.723PheAsp: 1.723 ± 0.314
1.539PheGlu: 1.539 ± 0.307
0.615PhePhe: 0.615 ± 0.196
2.339PheGly: 2.339 ± 0.366
0.554PheHis: 0.554 ± 0.154
1.846PheIle: 1.846 ± 0.255
1.416PheLys: 1.416 ± 0.37
2.277PheLeu: 2.277 ± 0.405
0.8PheMet: 0.8 ± 0.196
1.231PheAsn: 1.231 ± 0.306
1.477PhePro: 1.477 ± 0.356
0.739PheGln: 0.739 ± 0.191
2.277PheArg: 2.277 ± 0.332
3.323PheSer: 3.323 ± 0.565
2.031PheThr: 2.031 ± 0.31
2.708PheVal: 2.708 ± 0.414
0.739PheTrp: 0.739 ± 0.21
0.923PheTyr: 0.923 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
5.724GlyAla: 5.724 ± 0.722
0.862GlyCys: 0.862 ± 0.294
4.554GlyAsp: 4.554 ± 0.611
5.539GlyGlu: 5.539 ± 0.709
2.646GlyPhe: 2.646 ± 0.432
5.478GlyGly: 5.478 ± 0.784
0.862GlyHis: 0.862 ± 0.216
4.37GlyIle: 4.37 ± 0.504
4.431GlyLys: 4.431 ± 0.645
5.847GlyLeu: 5.847 ± 0.655
2.462GlyMet: 2.462 ± 0.306
2.585GlyAsn: 2.585 ± 0.258
2.831GlyPro: 2.831 ± 1.092
3.139GlyGln: 3.139 ± 0.522
3.754GlyArg: 3.754 ± 0.476
4.062GlySer: 4.062 ± 0.467
3.508GlyThr: 3.508 ± 0.499
5.478GlyVal: 5.478 ± 0.545
2.523GlyTrp: 2.523 ± 0.438
1.785GlyTyr: 1.785 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
1.231HisAla: 1.231 ± 0.304
0.431HisCys: 0.431 ± 0.18
0.862HisAsp: 0.862 ± 0.243
0.923HisGlu: 0.923 ± 0.196
0.554HisPhe: 0.554 ± 0.18
1.539HisGly: 1.539 ± 0.259
0.492HisHis: 0.492 ± 0.215
1.046HisIle: 1.046 ± 0.215
1.108HisLys: 1.108 ± 0.254
1.477HisLeu: 1.477 ± 0.366
0.246HisMet: 0.246 ± 0.128
0.739HisAsn: 0.739 ± 0.208
0.739HisPro: 0.739 ± 0.208
0.554HisGln: 0.554 ± 0.178
1.354HisArg: 1.354 ± 0.25
1.662HisSer: 1.662 ± 0.463
0.985HisThr: 0.985 ± 0.237
0.923HisVal: 0.923 ± 0.228
0.246HisTrp: 0.246 ± 0.129
0.923HisTyr: 0.923 ± 0.266
0.0HisXaa: 0.0 ± 0.0
Ile
5.662IleAla: 5.662 ± 0.641
1.108IleCys: 1.108 ± 0.222
3.385IleAsp: 3.385 ± 0.59
2.954IleGlu: 2.954 ± 0.438
0.8IlePhe: 0.8 ± 0.2
3.323IleGly: 3.323 ± 0.463
1.169IleHis: 1.169 ± 0.281
2.4IleIle: 2.4 ± 0.365
2.893IleLys: 2.893 ± 0.42
3.754IleLeu: 3.754 ± 0.633
0.8IleMet: 0.8 ± 0.202
3.077IleAsn: 3.077 ± 0.423
1.908IlePro: 1.908 ± 0.353
1.908IleGln: 1.908 ± 0.279
4.616IleArg: 4.616 ± 0.408
3.139IleSer: 3.139 ± 0.491
4.616IleThr: 4.616 ± 0.598
2.893IleVal: 2.893 ± 0.423
0.8IleTrp: 0.8 ± 0.243
1.231IleTyr: 1.231 ± 0.329
0.0IleXaa: 0.0 ± 0.0
Lys
6.155LysAla: 6.155 ± 0.692
0.431LysCys: 0.431 ± 0.18
2.339LysAsp: 2.339 ± 0.383
3.877LysGlu: 3.877 ± 0.588
0.8LysPhe: 0.8 ± 0.225
4.0LysGly: 4.0 ± 0.69
1.108LysHis: 1.108 ± 0.26
2.4LysIle: 2.4 ± 0.396
3.077LysLys: 3.077 ± 0.455
4.185LysLeu: 4.185 ± 0.549
1.846LysMet: 1.846 ± 0.332
2.523LysAsn: 2.523 ± 0.379
2.277LysPro: 2.277 ± 0.434
2.585LysGln: 2.585 ± 0.464
4.062LysArg: 4.062 ± 0.567
2.646LysSer: 2.646 ± 0.399
3.077LysThr: 3.077 ± 0.413
2.77LysVal: 2.77 ± 0.558
1.108LysTrp: 1.108 ± 0.265
1.231LysTyr: 1.231 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
9.601LeuAla: 9.601 ± 0.926
1.169LeuCys: 1.169 ± 0.264
3.939LeuAsp: 3.939 ± 0.416
4.493LeuGlu: 4.493 ± 0.57
2.831LeuPhe: 2.831 ± 0.392
4.185LeuGly: 4.185 ± 0.513
1.846LeuHis: 1.846 ± 0.39
4.185LeuIle: 4.185 ± 0.585
4.739LeuLys: 4.739 ± 0.514
7.447LeuLeu: 7.447 ± 0.908
2.585LeuMet: 2.585 ± 0.411
3.693LeuAsn: 3.693 ± 0.383
3.631LeuPro: 3.631 ± 0.645
3.262LeuGln: 3.262 ± 0.459
6.647LeuArg: 6.647 ± 0.771
5.908LeuSer: 5.908 ± 0.504
5.539LeuThr: 5.539 ± 0.449
4.985LeuVal: 4.985 ± 0.777
1.908LeuTrp: 1.908 ± 0.325
1.908LeuTyr: 1.908 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
3.447MetAla: 3.447 ± 0.433
0.308MetCys: 0.308 ± 0.114
1.046MetAsp: 1.046 ± 0.338
0.985MetGlu: 0.985 ± 0.306
0.923MetPhe: 0.923 ± 0.211
1.169MetGly: 1.169 ± 0.322
0.369MetHis: 0.369 ± 0.17
0.923MetIle: 0.923 ± 0.23
1.969MetLys: 1.969 ± 0.38
3.139MetLeu: 3.139 ± 0.368
1.108MetMet: 1.108 ± 0.315
1.169MetAsn: 1.169 ± 0.194
1.292MetPro: 1.292 ± 0.268
1.354MetGln: 1.354 ± 0.264
2.031MetArg: 2.031 ± 0.342
1.846MetSer: 1.846 ± 0.291
2.646MetThr: 2.646 ± 0.332
1.723MetVal: 1.723 ± 0.363
0.431MetTrp: 0.431 ± 0.176
0.431MetTyr: 0.431 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
4.0AsnAla: 4.0 ± 0.536
0.554AsnCys: 0.554 ± 0.165
2.031AsnAsp: 2.031 ± 0.296
2.4AsnGlu: 2.4 ± 0.488
1.231AsnPhe: 1.231 ± 0.428
4.185AsnGly: 4.185 ± 0.533
0.985AsnHis: 0.985 ± 0.251
2.216AsnIle: 2.216 ± 0.384
1.416AsnLys: 1.416 ± 0.286
2.893AsnLeu: 2.893 ± 0.391
1.231AsnMet: 1.231 ± 0.334
2.154AsnAsn: 2.154 ± 0.466
2.277AsnPro: 2.277 ± 0.36
1.846AsnGln: 1.846 ± 0.312
2.585AsnArg: 2.585 ± 0.457
2.154AsnSer: 2.154 ± 0.371
1.969AsnThr: 1.969 ± 0.432
2.031AsnVal: 2.031 ± 0.376
0.369AsnTrp: 0.369 ± 0.132
1.108AsnTyr: 1.108 ± 0.266
0.0AsnXaa: 0.0 ± 0.0
Pro
4.677ProAla: 4.677 ± 0.639
0.431ProCys: 0.431 ± 0.145
3.508ProAsp: 3.508 ± 0.476
3.877ProGlu: 3.877 ± 0.57
1.231ProPhe: 1.231 ± 0.242
3.016ProGly: 3.016 ± 0.374
0.8ProHis: 0.8 ± 0.234
1.108ProIle: 1.108 ± 0.261
1.6ProLys: 1.6 ± 0.432
3.016ProLeu: 3.016 ± 0.461
0.739ProMet: 0.739 ± 0.206
1.169ProAsn: 1.169 ± 0.294
1.539ProPro: 1.539 ± 0.345
1.846ProGln: 1.846 ± 0.312
1.846ProArg: 1.846 ± 0.383
2.893ProSer: 2.893 ± 0.414
1.477ProThr: 1.477 ± 0.31
3.754ProVal: 3.754 ± 0.544
0.923ProTrp: 0.923 ± 0.239
0.923ProTyr: 0.923 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
4.185GlnAla: 4.185 ± 0.766
0.8GlnCys: 0.8 ± 0.199
1.723GlnAsp: 1.723 ± 0.303
2.154GlnGlu: 2.154 ± 0.355
1.539GlnPhe: 1.539 ± 0.318
2.893GlnGly: 2.893 ± 0.449
1.046GlnHis: 1.046 ± 0.232
2.277GlnIle: 2.277 ± 0.403
2.216GlnLys: 2.216 ± 0.389
3.57GlnLeu: 3.57 ± 0.657
1.6GlnMet: 1.6 ± 0.329
1.046GlnAsn: 1.046 ± 0.354
1.354GlnPro: 1.354 ± 0.263
2.893GlnGln: 2.893 ± 0.488
3.57GlnArg: 3.57 ± 0.469
2.831GlnSer: 2.831 ± 0.369
2.216GlnThr: 2.216 ± 0.369
2.585GlnVal: 2.585 ± 0.406
0.492GlnTrp: 0.492 ± 0.203
1.6GlnTyr: 1.6 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
4.924ArgAla: 4.924 ± 0.516
1.231ArgCys: 1.231 ± 0.318
4.0ArgAsp: 4.0 ± 0.622
4.924ArgGlu: 4.924 ± 0.589
2.708ArgPhe: 2.708 ± 0.393
3.385ArgGly: 3.385 ± 0.491
1.969ArgHis: 1.969 ± 0.348
4.185ArgIle: 4.185 ± 0.51
4.124ArgLys: 4.124 ± 0.534
6.709ArgLeu: 6.709 ± 0.663
2.031ArgMet: 2.031 ± 0.323
2.708ArgAsn: 2.708 ± 0.413
2.154ArgPro: 2.154 ± 0.415
3.631ArgGln: 3.631 ± 0.567
6.093ArgArg: 6.093 ± 0.904
3.323ArgSer: 3.323 ± 0.54
3.939ArgThr: 3.939 ± 0.542
4.0ArgVal: 4.0 ± 0.561
1.416ArgTrp: 1.416 ± 0.277
2.031ArgTyr: 2.031 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
8.247SerAla: 8.247 ± 1.025
0.554SerCys: 0.554 ± 0.177
4.124SerAsp: 4.124 ± 0.438
4.185SerGlu: 4.185 ± 0.479
1.662SerPhe: 1.662 ± 0.295
5.293SerGly: 5.293 ± 0.606
0.8SerHis: 0.8 ± 0.227
2.523SerIle: 2.523 ± 0.414
2.954SerLys: 2.954 ± 0.507
6.032SerLeu: 6.032 ± 0.599
1.908SerMet: 1.908 ± 0.3
1.969SerAsn: 1.969 ± 0.302
2.77SerPro: 2.77 ± 0.373
3.016SerGln: 3.016 ± 0.367
3.754SerArg: 3.754 ± 0.486
4.493SerSer: 4.493 ± 0.642
2.585SerThr: 2.585 ± 0.506
5.047SerVal: 5.047 ± 0.567
0.615SerTrp: 0.615 ± 0.175
1.846SerTyr: 1.846 ± 0.388
0.0SerXaa: 0.0 ± 0.0
Thr
5.478ThrAla: 5.478 ± 0.783
0.739ThrCys: 0.739 ± 0.201
4.0ThrAsp: 4.0 ± 0.411
3.262ThrGlu: 3.262 ± 0.423
2.339ThrPhe: 2.339 ± 0.308
5.231ThrGly: 5.231 ± 0.769
1.108ThrHis: 1.108 ± 0.229
3.447ThrIle: 3.447 ± 0.522
2.646ThrLys: 2.646 ± 0.455
5.539ThrLeu: 5.539 ± 0.613
0.554ThrMet: 0.554 ± 0.201
1.6ThrAsn: 1.6 ± 0.288
3.2ThrPro: 3.2 ± 0.476
1.539ThrGln: 1.539 ± 0.297
2.462ThrArg: 2.462 ± 0.414
3.877ThrSer: 3.877 ± 0.496
3.139ThrThr: 3.139 ± 0.5
3.939ThrVal: 3.939 ± 0.632
1.354ThrTrp: 1.354 ± 0.277
1.846ThrTyr: 1.846 ± 0.405
0.0ThrXaa: 0.0 ± 0.0
Val
6.155ValAla: 6.155 ± 0.727
0.739ValCys: 0.739 ± 0.199
3.323ValAsp: 3.323 ± 0.478
4.0ValGlu: 4.0 ± 0.504
2.277ValPhe: 2.277 ± 0.425
3.385ValGly: 3.385 ± 0.51
0.739ValHis: 0.739 ± 0.208
3.57ValIle: 3.57 ± 0.447
3.754ValLys: 3.754 ± 0.571
5.478ValLeu: 5.478 ± 0.563
1.969ValMet: 1.969 ± 0.259
3.139ValAsn: 3.139 ± 0.458
3.016ValPro: 3.016 ± 0.424
2.462ValGln: 2.462 ± 0.395
4.062ValArg: 4.062 ± 0.471
4.493ValSer: 4.493 ± 0.596
4.616ValThr: 4.616 ± 0.692
4.37ValVal: 4.37 ± 0.508
1.046ValTrp: 1.046 ± 0.243
2.031ValTyr: 2.031 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
1.292TrpAla: 1.292 ± 0.203
0.369TrpCys: 0.369 ± 0.163
0.739TrpAsp: 0.739 ± 0.214
0.739TrpGlu: 0.739 ± 0.213
1.046TrpPhe: 1.046 ± 0.232
1.231TrpGly: 1.231 ± 0.236
0.492TrpHis: 0.492 ± 0.164
1.169TrpIle: 1.169 ± 0.279
1.292TrpLys: 1.292 ± 0.306
2.031TrpLeu: 2.031 ± 0.434
0.923TrpMet: 0.923 ± 0.212
0.492TrpAsn: 0.492 ± 0.173
1.108TrpPro: 1.108 ± 0.283
1.231TrpGln: 1.231 ± 0.244
1.846TrpArg: 1.846 ± 0.413
0.8TrpSer: 0.8 ± 0.224
0.862TrpThr: 0.862 ± 0.216
1.231TrpVal: 1.231 ± 0.292
0.554TrpTrp: 0.554 ± 0.206
0.554TrpTyr: 0.554 ± 0.194
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.363
0.308TyrCys: 0.308 ± 0.126
1.539TyrAsp: 1.539 ± 0.273
1.908TyrGlu: 1.908 ± 0.361
1.046TyrPhe: 1.046 ± 0.263
2.339TyrGly: 2.339 ± 0.371
0.431TyrHis: 0.431 ± 0.149
1.6TyrIle: 1.6 ± 0.295
1.046TyrLys: 1.046 ± 0.285
2.523TyrLeu: 2.523 ± 0.439
0.492TyrMet: 0.492 ± 0.201
1.169TyrAsn: 1.169 ± 0.287
0.923TyrPro: 0.923 ± 0.228
1.723TyrGln: 1.723 ± 0.326
2.216TyrArg: 2.216 ± 0.328
1.6TyrSer: 1.6 ± 0.334
1.6TyrThr: 1.6 ± 0.335
1.416TyrVal: 1.416 ± 0.371
0.677TyrTrp: 0.677 ± 0.211
0.8TyrTyr: 0.8 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (16249 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski