Actinomadura sp. KC06

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8327 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4X9Y8|A0A4R4X9Y8_9ACTN NUDIX hydrolase OS=Actinomadura sp. KC06 OX=2530369 GN=E1287_34695 PE=4 SV=1
MM1 pKa = 7.86AYY3 pKa = 8.73ATADD7 pKa = 3.83DD8 pKa = 4.74LDD10 pKa = 5.97DD11 pKa = 4.39YY12 pKa = 11.79VDD14 pKa = 3.87TVPANAARR22 pKa = 11.84LLTRR26 pKa = 11.84ASRR29 pKa = 11.84VIDD32 pKa = 3.87GALKK36 pKa = 10.26CAVYY40 pKa = 10.52DD41 pKa = 3.49VDD43 pKa = 4.25EE44 pKa = 5.93AGDD47 pKa = 3.74PTDD50 pKa = 4.5ADD52 pKa = 3.99VITALKK58 pKa = 10.66DD59 pKa = 3.45ATCEE63 pKa = 3.68QAAYY67 pKa = 9.26WITQGSDD74 pKa = 2.52EE75 pKa = 5.37GIPNGYY81 pKa = 7.77TSVSIGSVALAGGQTGNGGGPGTVSGEE108 pKa = 3.83LCAQARR114 pKa = 11.84QILDD118 pKa = 3.37TAGLLNFAPWTWW130 pKa = 2.98

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4X4F2|A0A4R4X4F2_9ACTN Uncharacterized protein OS=Actinomadura sp. KC06 OX=2530369 GN=E1287_37480 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.44GRR40 pKa = 11.84TRR42 pKa = 11.84IAVV45 pKa = 3.24

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8327

0

8327

2679231

20

5884

321.8

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.418 ± 0.042

0.804 ± 0.008

6.269 ± 0.022

5.67 ± 0.026

2.808 ± 0.015

9.554 ± 0.029

2.215 ± 0.012

3.523 ± 0.017

2.083 ± 0.021

10.179 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.893 ± 0.011

1.76 ± 0.015

6.215 ± 0.024

2.579 ± 0.016

8.538 ± 0.034

4.856 ± 0.017

5.663 ± 0.02

8.442 ± 0.027

1.511 ± 0.012

2.024 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski