Amino acid dipepetide frequency for Actinomadura sp. KC06

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.423AlaAla: 21.423 ± 0.152	1.093AlaCys: 1.093 ± 0.021	8.471AlaAsp: 8.471 ± 0.071	8.783AlaGlu: 8.783 ± 0.07	3.729AlaPhe: 3.729 ± 0.042	13.428AlaGly: 13.428 ± 0.087	2.624AlaHis: 2.624 ± 0.034	4.199AlaIle: 4.199 ± 0.042	2.825AlaLys: 2.825 ± 0.043	13.888AlaLeu: 13.888 ± 0.098	2.63AlaMet: 2.63 ± 0.029	1.92AlaAsn: 1.92 ± 0.032	6.555AlaPro: 6.555 ± 0.061	3.372AlaGln: 3.372 ± 0.045	10.774AlaArg: 10.774 ± 0.083	5.945AlaSer: 5.945 ± 0.053	6.41AlaThr: 6.41 ± 0.065	11.666AlaVal: 11.666 ± 0.087	1.879AlaTrp: 1.879 ± 0.03	2.571AlaTyr: 2.571 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.023CysAla: 1.023 ± 0.021	0.107CysCys: 0.107 ± 0.007	0.542CysAsp: 0.542 ± 0.015	0.432CysGlu: 0.432 ± 0.012	0.224CysPhe: 0.224 ± 0.008	0.989CysGly: 0.989 ± 0.022	0.215CysHis: 0.215 ± 0.009	0.171CysIle: 0.171 ± 0.009	0.132CysLys: 0.132 ± 0.008	0.701CysLeu: 0.701 ± 0.015	0.151CysMet: 0.151 ± 0.009	0.141CysAsn: 0.141 ± 0.007	0.519CysPro: 0.519 ± 0.017	0.175CysGln: 0.175 ± 0.008	0.664CysArg: 0.664 ± 0.019	0.426CysSer: 0.426 ± 0.013	0.455CysThr: 0.455 ± 0.013	0.67CysVal: 0.67 ± 0.016	0.12CysTrp: 0.12 ± 0.007	0.183CysTyr: 0.183 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.177AspAla: 8.177 ± 0.067	0.392AspCys: 0.392 ± 0.012	4.307AspAsp: 4.307 ± 0.046	4.406AspGlu: 4.406 ± 0.041	1.593AspPhe: 1.593 ± 0.024	6.923AspGly: 6.923 ± 0.08	1.431AspHis: 1.431 ± 0.022	1.87AspIle: 1.87 ± 0.028	1.049AspLys: 1.049 ± 0.022	6.745AspLeu: 6.745 ± 0.059	0.937AspMet: 0.937 ± 0.016	0.861AspAsn: 0.861 ± 0.021	4.691AspPro: 4.691 ± 0.043	1.604AspGln: 1.604 ± 0.028	5.32AspArg: 5.32 ± 0.046	2.06AspSer: 2.06 ± 0.028	2.669AspThr: 2.669 ± 0.032	5.569AspVal: 5.569 ± 0.055	0.93AspTrp: 0.93 ± 0.021	1.153AspTyr: 1.153 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.002GluAla: 7.002 ± 0.054	0.387GluCys: 0.387 ± 0.013	2.832GluAsp: 2.832 ± 0.036	3.237GluGlu: 3.237 ± 0.038	1.631GluPhe: 1.631 ± 0.023	3.993GluGly: 3.993 ± 0.039	1.561GluHis: 1.561 ± 0.026	2.671GluIle: 2.671 ± 0.035	1.301GluLys: 1.301 ± 0.027	6.536GluLeu: 6.536 ± 0.059	1.048GluMet: 1.048 ± 0.018	1.036GluAsn: 1.036 ± 0.019	3.731GluPro: 3.731 ± 0.042	1.99GluGln: 1.99 ± 0.031	5.968GluArg: 5.968 ± 0.048	2.503GluSer: 2.503 ± 0.035	2.997GluThr: 2.997 ± 0.037	4.325GluVal: 4.325 ± 0.046	0.832GluTrp: 0.832 ± 0.018	1.122GluTyr: 1.122 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.807PheAla: 3.807 ± 0.036	0.295PheCys: 0.295 ± 0.009	2.018PheAsp: 2.018 ± 0.033	1.473PheGlu: 1.473 ± 0.025	0.854PhePhe: 0.854 ± 0.021	3.18PheGly: 3.18 ± 0.038	0.633PheHis: 0.633 ± 0.016	0.807PheIle: 0.807 ± 0.019	0.515PheLys: 0.515 ± 0.016	2.608PheLeu: 2.608 ± 0.04	0.447PheMet: 0.447 ± 0.013	0.584PheAsn: 0.584 ± 0.017	1.428PhePro: 1.428 ± 0.024	0.72PheGln: 0.72 ± 0.017	1.921PheArg: 1.921 ± 0.026	1.422PheSer: 1.422 ± 0.025	1.968PheThr: 1.968 ± 0.027	2.349PheVal: 2.349 ± 0.031	0.434PheTrp: 0.434 ± 0.012	0.613PheTyr: 0.613 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.933GlyAla: 10.933 ± 0.098	0.839GlyCys: 0.839 ± 0.018	5.664GlyAsp: 5.664 ± 0.05	5.217GlyGlu: 5.217 ± 0.045	2.931GlyPhe: 2.931 ± 0.034	9.368GlyGly: 9.368 ± 0.089	2.263GlyHis: 2.263 ± 0.032	3.389GlyIle: 3.389 ± 0.037	2.368GlyLys: 2.368 ± 0.036	9.632GlyLeu: 9.632 ± 0.063	2.133GlyMet: 2.133 ± 0.028	1.678GlyAsn: 1.678 ± 0.033	5.556GlyPro: 5.556 ± 0.104	2.498GlyGln: 2.498 ± 0.036	8.699GlyArg: 8.699 ± 0.064	4.85GlySer: 4.85 ± 0.046	5.842GlyThr: 5.842 ± 0.051	7.782GlyVal: 7.782 ± 0.059	1.699GlyTrp: 1.699 ± 0.025	2.198GlyTyr: 2.198 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.683HisAla: 2.683 ± 0.035	0.21HisCys: 0.21 ± 0.009	1.441HisAsp: 1.441 ± 0.024	1.206HisGlu: 1.206 ± 0.025	0.581HisPhe: 0.581 ± 0.015	2.376HisGly: 2.376 ± 0.033	0.63HisHis: 0.63 ± 0.019	0.682HisIle: 0.682 ± 0.017	0.337HisLys: 0.337 ± 0.011	2.332HisLeu: 2.332 ± 0.03	0.381HisMet: 0.381 ± 0.013	0.369HisAsn: 0.369 ± 0.013	1.676HisPro: 1.676 ± 0.027	0.552HisGln: 0.552 ± 0.014	2.088HisArg: 2.088 ± 0.033	0.914HisSer: 0.914 ± 0.019	1.06HisThr: 1.06 ± 0.021	1.802HisVal: 1.802 ± 0.032	0.349HisTrp: 0.349 ± 0.011	0.481HisTyr: 0.481 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.295IleAla: 5.295 ± 0.052	0.335IleCys: 0.335 ± 0.012	2.498IleAsp: 2.498 ± 0.033	2.301IleGlu: 2.301 ± 0.036	0.854IlePhe: 0.854 ± 0.02	3.806IleGly: 3.806 ± 0.047	0.603IleHis: 0.603 ± 0.015	1.101IleIle: 1.101 ± 0.024	0.777IleLys: 0.777 ± 0.018	2.664IleLeu: 2.664 ± 0.035	0.571IleMet: 0.571 ± 0.014	0.749IleAsn: 0.749 ± 0.019	1.89IlePro: 1.89 ± 0.029	0.745IleGln: 0.745 ± 0.017	2.484IleArg: 2.484 ± 0.029	1.808IleSer: 1.808 ± 0.026	2.307IleThr: 2.307 ± 0.03	3.416IleVal: 3.416 ± 0.043	0.413IleTrp: 0.413 ± 0.012	0.613IleTyr: 0.613 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.814LysAla: 2.814 ± 0.039	0.123LysCys: 0.123 ± 0.007	1.225LysAsp: 1.225 ± 0.022	1.091LysGlu: 1.091 ± 0.023	0.452LysPhe: 0.452 ± 0.015	1.778LysGly: 1.778 ± 0.03	0.434LysHis: 0.434 ± 0.012	0.977LysIle: 0.977 ± 0.026	0.706LysLys: 0.706 ± 0.023	1.879LysLeu: 1.879 ± 0.034	0.386LysMet: 0.386 ± 0.011	0.505LysAsn: 0.505 ± 0.016	1.362LysPro: 1.362 ± 0.029	0.627LysGln: 0.627 ± 0.016	1.533LysArg: 1.533 ± 0.028	1.109LysSer: 1.109 ± 0.025	1.282LysThr: 1.282 ± 0.026	1.859LysVal: 1.859 ± 0.032	0.271LysTrp: 0.271 ± 0.011	0.413LysTyr: 0.413 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.15LeuAla: 15.15 ± 0.09	0.798LeuCys: 0.798 ± 0.019	7.154LeuAsp: 7.154 ± 0.058	4.783LeuGlu: 4.783 ± 0.055	2.578LeuPhe: 2.578 ± 0.038	9.189LeuGly: 9.189 ± 0.069	2.164LeuHis: 2.164 ± 0.033	3.703LeuIle: 3.703 ± 0.04	1.922LeuLys: 1.922 ± 0.029	10.513LeuLeu: 10.513 ± 0.085	1.7LeuMet: 1.7 ± 0.026	1.816LeuAsn: 1.816 ± 0.027	6.32LeuPro: 6.32 ± 0.054	2.126LeuGln: 2.126 ± 0.032	8.945LeuArg: 8.945 ± 0.074	5.048LeuSer: 5.048 ± 0.046	6.412LeuThr: 6.412 ± 0.052	8.426LeuVal: 8.426 ± 0.066	1.279LeuTrp: 1.279 ± 0.024	1.761LeuTyr: 1.761 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.467MetAla: 2.467 ± 0.03	0.143MetCys: 0.143 ± 0.008	1.04MetAsp: 1.04 ± 0.018	0.801MetGlu: 0.801 ± 0.016	0.531MetPhe: 0.531 ± 0.017	1.378MetGly: 1.378 ± 0.024	0.381MetHis: 0.381 ± 0.011	0.856MetIle: 0.856 ± 0.021	0.452MetLys: 0.452 ± 0.014	1.9MetLeu: 1.9 ± 0.027	0.322MetMet: 0.322 ± 0.012	0.455MetAsn: 0.455 ± 0.013	1.224MetPro: 1.224 ± 0.022	0.44MetGln: 0.44 ± 0.012	1.671MetArg: 1.671 ± 0.024	1.338MetSer: 1.338 ± 0.025	1.587MetThr: 1.587 ± 0.027	1.381MetVal: 1.381 ± 0.026	0.242MetTrp: 0.242 ± 0.01	0.314MetTyr: 0.314 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.275AsnAla: 2.275 ± 0.032	0.186AsnCys: 0.186 ± 0.008	0.964AsnAsp: 0.964 ± 0.024	0.88AsnGlu: 0.88 ± 0.021	0.486AsnPhe: 0.486 ± 0.016	1.91AsnGly: 1.91 ± 0.034	0.363AsnHis: 0.363 ± 0.011	0.629AsnIle: 0.629 ± 0.017	0.372AsnLys: 0.372 ± 0.012	1.713AsnLeu: 1.713 ± 0.025	0.297AsnMet: 0.297 ± 0.011	0.357AsnAsn: 0.357 ± 0.012	1.364AsnPro: 1.364 ± 0.023	0.484AsnGln: 0.484 ± 0.014	1.33AsnArg: 1.33 ± 0.025	0.798AsnSer: 0.798 ± 0.02	0.998AsnThr: 0.998 ± 0.023	1.536AsnVal: 1.536 ± 0.026	0.262AsnTrp: 0.262 ± 0.01	0.39AsnTyr: 0.39 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.343ProAla: 8.343 ± 0.081	0.377ProCys: 0.377 ± 0.015	4.926ProAsp: 4.926 ± 0.048	4.199ProGlu: 4.199 ± 0.042	1.642ProPhe: 1.642 ± 0.028	7.112ProGly: 7.112 ± 0.062	1.306ProHis: 1.306 ± 0.026	1.72ProIle: 1.72 ± 0.027	1.246ProLys: 1.246 ± 0.029	5.187ProLeu: 5.187 ± 0.045	1.184ProMet: 1.184 ± 0.02	0.996ProAsn: 0.996 ± 0.022	4.386ProPro: 4.386 ± 0.068	1.545ProGln: 1.545 ± 0.026	4.17ProArg: 4.17 ± 0.048	3.565ProSer: 3.565 ± 0.04	2.77ProThr: 2.77 ± 0.047	5.216ProVal: 5.216 ± 0.05	0.932ProTrp: 0.932 ± 0.019	1.323ProTyr: 1.323 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.589GlnAla: 3.589 ± 0.042	0.168GlnCys: 0.168 ± 0.008	1.393GlnAsp: 1.393 ± 0.021	1.306GlnGlu: 1.306 ± 0.024	0.662GlnPhe: 0.662 ± 0.017	2.079GlnGly: 2.079 ± 0.031	0.557GlnHis: 0.557 ± 0.013	1.187GlnIle: 1.187 ± 0.024	0.607GlnLys: 0.607 ± 0.018	2.479GlnLeu: 2.479 ± 0.036	0.524GlnMet: 0.524 ± 0.015	0.509GlnAsn: 0.509 ± 0.014	1.498GlnPro: 1.498 ± 0.029	1.036GlnGln: 1.036 ± 0.034	2.375GlnArg: 2.375 ± 0.031	1.153GlnSer: 1.153 ± 0.024	1.341GlnThr: 1.341 ± 0.024	2.391GlnVal: 2.391 ± 0.031	0.409GlnTrp: 0.409 ± 0.013	0.524GlnTyr: 0.524 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.24ArgAla: 10.24 ± 0.081	0.656ArgCys: 0.656 ± 0.017	5.169ArgAsp: 5.169 ± 0.04	4.83ArgGlu: 4.83 ± 0.051	2.532ArgPhe: 2.532 ± 0.032	6.088ArgGly: 6.088 ± 0.052	2.193ArgHis: 2.193 ± 0.034	3.316ArgIle: 3.316 ± 0.039	1.685ArgLys: 1.685 ± 0.027	9.766ArgLeu: 9.766 ± 0.074	1.918ArgMet: 1.918 ± 0.027	1.458ArgAsn: 1.458 ± 0.026	5.644ArgPro: 5.644 ± 0.053	2.233ArgGln: 2.233 ± 0.028	8.813ArgArg: 8.813 ± 0.084	4.034ArgSer: 4.034 ± 0.038	5.051ArgThr: 5.051 ± 0.048	6.376ArgVal: 6.376 ± 0.054	1.49ArgTrp: 1.49 ± 0.026	1.884ArgTyr: 1.884 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.219SerAla: 6.219 ± 0.05	0.385SerCys: 0.385 ± 0.013	2.695SerAsp: 2.695 ± 0.031	2.339SerGlu: 2.339 ± 0.032	1.501SerPhe: 1.501 ± 0.025	5.975SerGly: 5.975 ± 0.05	0.942SerHis: 0.942 ± 0.022	1.628SerIle: 1.628 ± 0.025	0.985SerLys: 0.985 ± 0.025	4.57SerLeu: 4.57 ± 0.051	1.15SerMet: 1.15 ± 0.022	0.811SerAsn: 0.811 ± 0.02	3.217SerPro: 3.217 ± 0.038	1.176SerGln: 1.176 ± 0.022	3.832SerArg: 3.832 ± 0.042	2.647SerSer: 2.647 ± 0.03	2.607SerThr: 2.607 ± 0.038	3.933SerVal: 3.933 ± 0.039	0.822SerTrp: 0.822 ± 0.019	1.124SerTyr: 1.124 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	7.809ThrAla: 7.809 ± 0.056	0.452ThrCys: 0.452 ± 0.013	3.123ThrAsp: 3.123 ± 0.035	2.837ThrGlu: 2.837 ± 0.035	1.686ThrPhe: 1.686 ± 0.03	6.449ThrGly: 6.449 ± 0.079	1.072ThrHis: 1.072 ± 0.025	2.007ThrIle: 2.007 ± 0.029	1.068ThrLys: 1.068 ± 0.025	5.391ThrLeu: 5.391 ± 0.043	1.065ThrMet: 1.065 ± 0.02	0.906ThrAsn: 0.906 ± 0.016	3.676ThrPro: 3.676 ± 0.039	1.198ThrGln: 1.198 ± 0.025	4.081ThrArg: 4.081 ± 0.043	2.987ThrSer: 2.987 ± 0.034	3.228ThrThr: 3.228 ± 0.041	5.454ThrVal: 5.454 ± 0.051	0.911ThrTrp: 0.911 ± 0.021	1.231ThrTyr: 1.231 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	10.825ValAla: 10.825 ± 0.085	0.737ValCys: 0.737 ± 0.014	4.93ValAsp: 4.93 ± 0.045	4.628ValGlu: 4.628 ± 0.044	2.491ValPhe: 2.491 ± 0.034	6.306ValGly: 6.306 ± 0.054	1.989ValHis: 1.989 ± 0.027	3.209ValIle: 3.209 ± 0.039	1.725ValLys: 1.725 ± 0.032	9.467ValLeu: 9.467 ± 0.067	1.516ValMet: 1.516 ± 0.023	1.672ValAsn: 1.672 ± 0.027	5.453ValPro: 5.453 ± 0.053	2.209ValGln: 2.209 ± 0.029	7.3ValArg: 7.3 ± 0.063	4.164ValSer: 4.164 ± 0.041	5.417ValThr: 5.417 ± 0.045	7.714ValVal: 7.714 ± 0.069	1.128ValTrp: 1.128 ± 0.021	1.536ValTyr: 1.536 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.568TrpAla: 1.568 ± 0.023	0.159TrpCys: 0.159 ± 0.008	0.878TrpAsp: 0.878 ± 0.018	0.724TrpGlu: 0.724 ± 0.017	0.478TrpPhe: 0.478 ± 0.014	1.041TrpGly: 1.041 ± 0.02	0.372TrpHis: 0.372 ± 0.012	0.58TrpIle: 0.58 ± 0.015	0.361TrpLys: 0.361 ± 0.012	1.726TrpLeu: 1.726 ± 0.027	0.283TrpMet: 0.283 ± 0.01	0.391TrpAsn: 0.391 ± 0.014	0.891TrpPro: 0.891 ± 0.018	0.483TrpGln: 0.483 ± 0.015	1.547TrpArg: 1.547 ± 0.029	0.882TrpSer: 0.882 ± 0.025	1.118TrpThr: 1.118 ± 0.02	0.941TrpVal: 0.941 ± 0.02	0.345TrpTrp: 0.345 ± 0.011	0.34TrpTyr: 0.34 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.543TyrAla: 2.543 ± 0.032	0.196TyrCys: 0.196 ± 0.009	1.42TyrAsp: 1.42 ± 0.034	1.23TyrGlu: 1.23 ± 0.023	0.642TyrPhe: 0.642 ± 0.017	2.167TyrGly: 2.167 ± 0.032	0.415TyrHis: 0.415 ± 0.013	0.529TyrIle: 0.529 ± 0.015	0.393TyrLys: 0.393 ± 0.012	2.092TyrLeu: 2.092 ± 0.026	0.28TyrMet: 0.28 ± 0.011	0.381TyrAsn: 0.381 ± 0.013	1.063TyrPro: 1.063 ± 0.019	0.574TyrGln: 0.574 ± 0.014	1.86TyrArg: 1.86 ± 0.026	0.904TyrSer: 0.904 ± 0.02	1.112TyrThr: 1.112 ± 0.022	1.613TyrVal: 1.613 ± 0.029	0.359TyrTrp: 0.359 ± 0.013	0.462TyrTyr: 0.462 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8327 proteins (2679231 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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