Streptococcus phage Javan101

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXE3|A0A4D5ZXE3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan101 OX=2547974 GN=Javan101_0034 PE=4 SV=1
MM1 pKa = 6.73TTQTTLEE8 pKa = 4.23NAYY11 pKa = 10.65SLYY14 pKa = 10.07PATASIVPFKK24 pKa = 10.64NWLIIAYY31 pKa = 9.56QSYY34 pKa = 10.74KK35 pKa = 10.59GINLHH40 pKa = 6.32IFEE43 pKa = 4.63TVEE46 pKa = 3.87NLDD49 pKa = 3.71EE50 pKa = 4.82FPQEE54 pKa = 3.65EE55 pKa = 4.38RR56 pKa = 11.84RR57 pKa = 11.84FNLIIDD63 pKa = 4.04SEE65 pKa = 4.66EE66 pKa = 3.99TFQDD70 pKa = 2.71QGHH73 pKa = 5.34AVKK76 pKa = 9.62WAFEE80 pKa = 4.1TLGAA84 pKa = 4.58

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZZN3|A0A4D5ZZN3_9CAUD Holin OS=Streptococcus phage Javan101 OX=2547974 GN=Javan101_0006 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 5.24IYY4 pKa = 10.94TILLCTILGIYY15 pKa = 9.46VFLGLYY21 pKa = 9.52LNYY24 pKa = 8.2MTIRR28 pKa = 11.84DD29 pKa = 4.0DD30 pKa = 3.09IRR32 pKa = 11.84RR33 pKa = 11.84EE34 pKa = 3.9KK35 pKa = 10.04EE36 pKa = 3.45RR37 pKa = 11.84KK38 pKa = 9.3AEE40 pKa = 4.37KK41 pKa = 10.04KK42 pKa = 10.48RR43 pKa = 11.84PDD45 pKa = 3.54NNTTPLHH52 pKa = 6.53RR53 pKa = 11.84SRR55 pKa = 5.13

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

11683

37

1039

299.6

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.027 ± 0.486

0.89 ± 0.169

5.872 ± 0.375

7.181 ± 0.362

3.604 ± 0.197

6.95 ± 0.412

2.029 ± 0.159

5.803 ± 0.292

7.344 ± 0.273

9.244 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.157

4.28 ± 0.225

3.056 ± 0.243

4.434 ± 0.24

4.691 ± 0.443

6.813 ± 0.385

6.933 ± 0.492

6.48 ± 0.258

1.429 ± 0.089

3.604 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski