Pseudomonas phage MR2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; unclassified Ghunavirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T8T9|A0A6M3T8T9_9CAUD DNA ligase OS=Pseudomonas phage MR2 OX=2711170 GN=PssvBMR2_gp15 PE=4 SV=1
MM1 pKa = 7.26LAIVQHH7 pKa = 6.18YY8 pKa = 8.65IQNPDD13 pKa = 4.95DD14 pKa = 4.38IPDD17 pKa = 3.65IAQASAEE24 pKa = 4.06YY25 pKa = 11.14LNVRR29 pKa = 11.84LNASYY34 pKa = 10.89LIATGMVDD42 pKa = 3.28EE43 pKa = 4.95LRR45 pKa = 11.84RR46 pKa = 11.84SGMSEE51 pKa = 3.58QFIAGFLEE59 pKa = 4.56GCNAATEE66 pKa = 4.68IIEE69 pKa = 4.9LMQEE73 pKa = 4.07SQRR76 pKa = 11.84QQEE79 pKa = 4.35DD80 pKa = 3.11

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TCK3|A0A6M3TCK3_9CAUD Putative DNA-directed RNA polymerase OS=Pseudomonas phage MR2 OX=2711170 GN=PssvBMR2_gp12 PE=4 SV=1
MM1 pKa = 7.15TRR3 pKa = 11.84QGGPKK8 pKa = 10.07DD9 pKa = 3.58RR10 pKa = 11.84VKK12 pKa = 11.02EE13 pKa = 3.97GLDD16 pKa = 3.23KK17 pKa = 10.99VVKK20 pKa = 9.89RR21 pKa = 11.84CRR23 pKa = 11.84IRR25 pKa = 11.84NINNEE30 pKa = 4.15TRR32 pKa = 11.84CQRR35 pKa = 11.84WTT37 pKa = 3.25

Molecular weight: 4.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12926

37

1399

235.0

26.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 0.471

0.874 ± 0.138

6.622 ± 0.271

6.607 ± 0.471

3.644 ± 0.215

7.806 ± 0.337

2.004 ± 0.211

4.905 ± 0.209

5.864 ± 0.343

8.115 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.173

4.054 ± 0.289

3.868 ± 0.242

4.464 ± 0.325

5.934 ± 0.26

6.096 ± 0.46

5.562 ± 0.297

6.537 ± 0.303

1.532 ± 0.132

3.048 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski