Amino acid dipepetide frequency for Pseudomonas phage MR2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.141AlaAla: 11.141 ± 1.281
0.696AlaCys: 0.696 ± 0.251
5.803AlaAsp: 5.803 ± 0.582
5.648AlaGlu: 5.648 ± 0.603
3.095AlaPhe: 3.095 ± 0.519
7.505AlaGly: 7.505 ± 0.648
1.393AlaHis: 1.393 ± 0.339
5.88AlaIle: 5.88 ± 0.705
5.88AlaLys: 5.88 ± 0.56
7.041AlaLeu: 7.041 ± 0.931
3.327AlaMet: 3.327 ± 0.577
4.487AlaAsn: 4.487 ± 0.65
3.714AlaPro: 3.714 ± 0.558
4.952AlaGln: 4.952 ± 0.601
5.88AlaArg: 5.88 ± 0.728
7.814AlaSer: 7.814 ± 1.117
5.106AlaThr: 5.106 ± 0.716
4.797AlaVal: 4.797 ± 0.55
1.393AlaTrp: 1.393 ± 0.467
2.553AlaTyr: 2.553 ± 0.495
0.0AlaXaa: 0.0 ± 0.0
Cys
1.083CysAla: 1.083 ± 0.304
0.0CysCys: 0.0 ± 0.0
0.542CysAsp: 0.542 ± 0.291
0.464CysGlu: 0.464 ± 0.252
0.696CysPhe: 0.696 ± 0.25
0.542CysGly: 0.542 ± 0.22
0.464CysHis: 0.464 ± 0.17
0.387CysIle: 0.387 ± 0.167
0.696CysLys: 0.696 ± 0.231
0.696CysLeu: 0.696 ± 0.218
0.232CysMet: 0.232 ± 0.15
0.232CysAsn: 0.232 ± 0.138
0.309CysPro: 0.309 ± 0.163
0.464CysGln: 0.464 ± 0.201
0.696CysArg: 0.696 ± 0.264
0.309CysSer: 0.309 ± 0.143
0.077CysThr: 0.077 ± 0.08
0.542CysVal: 0.542 ± 0.23
0.077CysTrp: 0.077 ± 0.088
0.232CysTyr: 0.232 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
6.19AspAla: 6.19 ± 0.554
0.387AspCys: 0.387 ± 0.168
5.029AspAsp: 5.029 ± 0.678
4.72AspGlu: 4.72 ± 0.722
2.785AspPhe: 2.785 ± 0.37
6.035AspGly: 6.035 ± 0.544
1.238AspHis: 1.238 ± 0.338
3.482AspIle: 3.482 ± 0.432
3.559AspLys: 3.559 ± 0.461
5.106AspLeu: 5.106 ± 0.719
2.012AspMet: 2.012 ± 0.301
2.166AspAsn: 2.166 ± 0.323
2.94AspPro: 2.94 ± 0.623
2.785AspGln: 2.785 ± 0.484
3.559AspArg: 3.559 ± 0.566
3.868AspSer: 3.868 ± 0.655
2.785AspThr: 2.785 ± 0.443
4.41AspVal: 4.41 ± 0.593
1.083AspTrp: 1.083 ± 0.337
2.089AspTyr: 2.089 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
8.201GluAla: 8.201 ± 0.968
0.774GluCys: 0.774 ± 0.248
4.565GluAsp: 4.565 ± 0.495
3.868GluGlu: 3.868 ± 0.76
3.017GluPhe: 3.017 ± 0.61
5.184GluGly: 5.184 ± 0.735
1.238GluHis: 1.238 ± 0.278
3.017GluIle: 3.017 ± 0.448
2.94GluLys: 2.94 ± 0.494
4.642GluLeu: 4.642 ± 0.568
2.166GluMet: 2.166 ± 0.305
2.553GluAsn: 2.553 ± 0.392
2.089GluPro: 2.089 ± 0.387
3.017GluGln: 3.017 ± 0.442
4.023GluArg: 4.023 ± 0.645
3.172GluSer: 3.172 ± 0.513
4.255GluThr: 4.255 ± 0.648
3.868GluVal: 3.868 ± 0.765
1.006GluTrp: 1.006 ± 0.277
2.476GluTyr: 2.476 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
3.017PheAla: 3.017 ± 0.402
0.387PheCys: 0.387 ± 0.157
2.631PheAsp: 2.631 ± 0.538
2.553PheGlu: 2.553 ± 0.564
1.238PhePhe: 1.238 ± 0.342
2.785PheGly: 2.785 ± 0.493
1.006PheHis: 1.006 ± 0.246
1.393PheIle: 1.393 ± 0.395
1.779PheLys: 1.779 ± 0.426
2.708PheLeu: 2.708 ± 0.378
1.238PheMet: 1.238 ± 0.299
1.779PheAsn: 1.779 ± 0.432
1.934PhePro: 1.934 ± 0.415
1.238PheGln: 1.238 ± 0.326
1.47PheArg: 1.47 ± 0.294
2.476PheSer: 2.476 ± 0.482
2.94PheThr: 2.94 ± 0.45
2.476PheVal: 2.476 ± 0.396
0.542PheTrp: 0.542 ± 0.239
0.851PheTyr: 0.851 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
6.963GlyAla: 6.963 ± 1.04
0.696GlyCys: 0.696 ± 0.272
4.797GlyAsp: 4.797 ± 0.584
4.72GlyGlu: 4.72 ± 0.537
3.172GlyPhe: 3.172 ± 0.416
6.422GlyGly: 6.422 ± 0.766
1.547GlyHis: 1.547 ± 0.435
4.333GlyIle: 4.333 ± 0.496
3.559GlyLys: 3.559 ± 0.705
6.499GlyLeu: 6.499 ± 0.944
1.857GlyMet: 1.857 ± 0.375
2.863GlyAsn: 2.863 ± 0.447
2.708GlyPro: 2.708 ± 0.422
3.404GlyGln: 3.404 ± 0.501
4.72GlyArg: 4.72 ± 0.59
5.416GlySer: 5.416 ± 0.736
5.029GlyThr: 5.029 ± 0.575
4.797GlyVal: 4.797 ± 0.701
1.547GlyTrp: 1.547 ± 0.347
3.017GlyTyr: 3.017 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.238HisAla: 1.238 ± 0.298
0.542HisCys: 0.542 ± 0.241
1.083HisAsp: 1.083 ± 0.272
1.161HisGlu: 1.161 ± 0.288
0.542HisPhe: 0.542 ± 0.171
2.012HisGly: 2.012 ± 0.374
0.155HisHis: 0.155 ± 0.121
1.006HisIle: 1.006 ± 0.261
0.928HisLys: 0.928 ± 0.301
2.244HisLeu: 2.244 ± 0.527
0.928HisMet: 0.928 ± 0.244
0.928HisAsn: 0.928 ± 0.27
1.006HisPro: 1.006 ± 0.386
0.542HisGln: 0.542 ± 0.202
1.006HisArg: 1.006 ± 0.303
1.006HisSer: 1.006 ± 0.352
0.696HisThr: 0.696 ± 0.22
1.547HisVal: 1.547 ± 0.365
0.542HisTrp: 0.542 ± 0.221
0.928HisTyr: 0.928 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
4.333IleAla: 4.333 ± 0.497
0.696IleCys: 0.696 ± 0.259
3.095IleAsp: 3.095 ± 0.439
3.714IleGlu: 3.714 ± 0.537
1.083IlePhe: 1.083 ± 0.268
3.017IleGly: 3.017 ± 0.402
1.161IleHis: 1.161 ± 0.349
2.785IleIle: 2.785 ± 0.412
3.017IleLys: 3.017 ± 0.428
4.41IleLeu: 4.41 ± 0.584
0.851IleMet: 0.851 ± 0.276
2.785IleAsn: 2.785 ± 0.586
2.089IlePro: 2.089 ± 0.432
2.012IleGln: 2.012 ± 0.414
3.559IleArg: 3.559 ± 0.48
2.476IleSer: 2.476 ± 0.383
2.553IleThr: 2.553 ± 0.513
3.095IleVal: 3.095 ± 0.399
0.696IleTrp: 0.696 ± 0.246
1.625IleTyr: 1.625 ± 0.353
0.0IleXaa: 0.0 ± 0.0
Lys
7.427LysAla: 7.427 ± 0.828
0.155LysCys: 0.155 ± 0.108
4.952LysAsp: 4.952 ± 0.817
4.023LysGlu: 4.023 ± 0.565
1.934LysPhe: 1.934 ± 0.374
4.101LysGly: 4.101 ± 0.726
1.315LysHis: 1.315 ± 0.474
2.553LysIle: 2.553 ± 0.477
2.94LysLys: 2.94 ± 0.509
4.797LysLeu: 4.797 ± 0.679
1.238LysMet: 1.238 ± 0.233
1.934LysAsn: 1.934 ± 0.342
2.708LysPro: 2.708 ± 0.552
2.631LysGln: 2.631 ± 0.587
3.482LysArg: 3.482 ± 0.567
1.625LysSer: 1.625 ± 0.329
3.25LysThr: 3.25 ± 0.538
5.184LysVal: 5.184 ± 0.741
0.851LysTrp: 0.851 ± 0.233
1.547LysTyr: 1.547 ± 0.325
0.0LysXaa: 0.0 ± 0.0
Leu
7.892LeuAla: 7.892 ± 0.85
0.464LeuCys: 0.464 ± 0.201
5.184LeuAsp: 5.184 ± 0.525
5.648LeuGlu: 5.648 ± 0.678
2.012LeuPhe: 2.012 ± 0.374
4.72LeuGly: 4.72 ± 0.487
1.702LeuHis: 1.702 ± 0.283
4.101LeuIle: 4.101 ± 0.618
6.112LeuLys: 6.112 ± 0.627
4.72LeuLeu: 4.72 ± 0.597
2.398LeuMet: 2.398 ± 0.415
3.636LeuAsn: 3.636 ± 0.614
2.863LeuPro: 2.863 ± 0.427
4.874LeuGln: 4.874 ± 0.544
5.725LeuArg: 5.725 ± 0.771
5.261LeuSer: 5.261 ± 0.67
5.029LeuThr: 5.029 ± 0.704
4.952LeuVal: 4.952 ± 0.556
1.315LeuTrp: 1.315 ± 0.384
2.553LeuTyr: 2.553 ± 0.454
0.0LeuXaa: 0.0 ± 0.0
Met
3.017MetAla: 3.017 ± 0.423
0.155MetCys: 0.155 ± 0.122
2.321MetAsp: 2.321 ± 0.412
1.779MetGlu: 1.779 ± 0.485
0.696MetPhe: 0.696 ± 0.274
2.012MetGly: 2.012 ± 0.328
0.309MetHis: 0.309 ± 0.197
1.47MetIle: 1.47 ± 0.292
1.315MetLys: 1.315 ± 0.315
2.553MetLeu: 2.553 ± 0.314
0.464MetMet: 0.464 ± 0.197
1.238MetAsn: 1.238 ± 0.308
1.315MetPro: 1.315 ± 0.247
1.238MetGln: 1.238 ± 0.407
1.238MetArg: 1.238 ± 0.266
1.934MetSer: 1.934 ± 0.383
1.857MetThr: 1.857 ± 0.398
1.238MetVal: 1.238 ± 0.288
0.309MetTrp: 0.309 ± 0.142
0.077MetTyr: 0.077 ± 0.073
0.0MetXaa: 0.0 ± 0.0
Asn
3.714AsnAla: 3.714 ± 0.591
0.309AsnCys: 0.309 ± 0.143
2.012AsnAsp: 2.012 ± 0.332
3.172AsnGlu: 3.172 ± 0.6
1.547AsnPhe: 1.547 ± 0.316
4.023AsnGly: 4.023 ± 0.724
0.774AsnHis: 0.774 ± 0.265
2.089AsnIle: 2.089 ± 0.369
1.393AsnLys: 1.393 ± 0.431
3.172AsnLeu: 3.172 ± 0.471
0.696AsnMet: 0.696 ± 0.25
2.166AsnAsn: 2.166 ± 0.427
2.244AsnPro: 2.244 ± 0.376
1.702AsnGln: 1.702 ± 0.323
2.089AsnArg: 2.089 ± 0.413
3.404AsnSer: 3.404 ± 0.872
1.857AsnThr: 1.857 ± 0.346
1.934AsnVal: 1.934 ± 0.435
0.619AsnTrp: 0.619 ± 0.214
1.625AsnTyr: 1.625 ± 0.293
0.0AsnXaa: 0.0 ± 0.0
Pro
2.631ProAla: 2.631 ± 0.504
0.387ProCys: 0.387 ± 0.193
3.791ProAsp: 3.791 ± 0.541
3.25ProGlu: 3.25 ± 0.483
1.702ProPhe: 1.702 ± 0.348
2.166ProGly: 2.166 ± 0.539
0.928ProHis: 0.928 ± 0.225
1.547ProIle: 1.547 ± 0.337
2.708ProLys: 2.708 ± 0.448
2.785ProLeu: 2.785 ± 0.578
1.006ProMet: 1.006 ± 0.343
1.934ProAsn: 1.934 ± 0.399
0.928ProPro: 0.928 ± 0.197
1.47ProGln: 1.47 ± 0.4
2.244ProArg: 2.244 ± 0.576
1.857ProSer: 1.857 ± 0.33
2.012ProThr: 2.012 ± 0.419
3.559ProVal: 3.559 ± 0.578
0.542ProTrp: 0.542 ± 0.2
1.238ProTyr: 1.238 ± 0.303
0.0ProXaa: 0.0 ± 0.0
Gln
5.648GlnAla: 5.648 ± 0.807
0.309GlnCys: 0.309 ± 0.117
2.708GlnAsp: 2.708 ± 0.435
3.559GlnGlu: 3.559 ± 0.604
2.012GlnPhe: 2.012 ± 0.312
3.714GlnGly: 3.714 ± 0.556
0.774GlnHis: 0.774 ± 0.263
1.779GlnIle: 1.779 ± 0.419
1.779GlnLys: 1.779 ± 0.352
4.41GlnLeu: 4.41 ± 0.476
1.238GlnMet: 1.238 ± 0.257
1.47GlnAsn: 1.47 ± 0.353
0.851GlnPro: 0.851 ± 0.211
2.012GlnGln: 2.012 ± 0.438
3.636GlnArg: 3.636 ± 0.695
2.476GlnSer: 2.476 ± 0.437
1.779GlnThr: 1.779 ± 0.461
2.631GlnVal: 2.631 ± 0.34
0.619GlnTrp: 0.619 ± 0.183
1.238GlnTyr: 1.238 ± 0.377
0.0GlnXaa: 0.0 ± 0.0
Arg
4.952ArgAla: 4.952 ± 0.514
0.696ArgCys: 0.696 ± 0.304
3.714ArgAsp: 3.714 ± 0.605
4.101ArgGlu: 4.101 ± 0.652
2.012ArgPhe: 2.012 ± 0.452
4.642ArgGly: 4.642 ± 0.576
1.238ArgHis: 1.238 ± 0.347
2.785ArgIle: 2.785 ± 0.571
4.178ArgLys: 4.178 ± 0.671
5.803ArgLeu: 5.803 ± 0.631
1.238ArgMet: 1.238 ± 0.287
1.702ArgAsn: 1.702 ± 0.363
1.625ArgPro: 1.625 ± 0.418
2.94ArgGln: 2.94 ± 0.319
3.559ArgArg: 3.559 ± 0.457
3.559ArgSer: 3.559 ± 0.558
3.327ArgThr: 3.327 ± 0.584
4.023ArgVal: 4.023 ± 0.568
1.083ArgTrp: 1.083 ± 0.392
2.166ArgTyr: 2.166 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
6.344SerAla: 6.344 ± 1.365
0.542SerCys: 0.542 ± 0.233
4.178SerAsp: 4.178 ± 0.478
3.404SerGlu: 3.404 ± 0.41
2.553SerPhe: 2.553 ± 0.471
5.261SerGly: 5.261 ± 0.801
1.315SerHis: 1.315 ± 0.265
3.172SerIle: 3.172 ± 0.518
3.636SerLys: 3.636 ± 0.634
4.487SerLeu: 4.487 ± 0.507
1.238SerMet: 1.238 ± 0.244
2.244SerAsn: 2.244 ± 0.446
2.012SerPro: 2.012 ± 0.369
3.25SerGln: 3.25 ± 0.535
2.94SerArg: 2.94 ± 0.474
4.333SerSer: 4.333 ± 0.91
3.017SerThr: 3.017 ± 0.673
3.791SerVal: 3.791 ± 0.558
1.006SerTrp: 1.006 ± 0.273
2.244SerTyr: 2.244 ± 0.4
0.0SerXaa: 0.0 ± 0.0
Thr
4.255ThrAla: 4.255 ± 0.674
0.309ThrCys: 0.309 ± 0.151
3.946ThrAsp: 3.946 ± 0.583
2.785ThrGlu: 2.785 ± 0.504
2.94ThrPhe: 2.94 ± 0.5
4.72ThrGly: 4.72 ± 0.556
0.464ThrHis: 0.464 ± 0.17
3.095ThrIle: 3.095 ± 0.589
4.565ThrLys: 4.565 ± 0.469
5.725ThrLeu: 5.725 ± 0.656
1.393ThrMet: 1.393 ± 0.29
1.857ThrAsn: 1.857 ± 0.398
2.863ThrPro: 2.863 ± 0.458
1.702ThrGln: 1.702 ± 0.33
2.321ThrArg: 2.321 ± 0.511
3.25ThrSer: 3.25 ± 0.661
3.095ThrThr: 3.095 ± 0.6
4.487ThrVal: 4.487 ± 0.66
0.542ThrTrp: 0.542 ± 0.143
1.315ThrTyr: 1.315 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
5.957ValAla: 5.957 ± 1.038
0.696ValCys: 0.696 ± 0.257
3.172ValAsp: 3.172 ± 0.455
4.178ValGlu: 4.178 ± 0.656
1.934ValPhe: 1.934 ± 0.45
4.874ValGly: 4.874 ± 0.568
1.47ValHis: 1.47 ± 0.454
2.631ValIle: 2.631 ± 0.44
4.333ValLys: 4.333 ± 0.762
4.797ValLeu: 4.797 ± 0.683
1.779ValMet: 1.779 ± 0.403
2.708ValAsn: 2.708 ± 0.707
3.25ValPro: 3.25 ± 0.477
2.785ValGln: 2.785 ± 0.477
4.487ValArg: 4.487 ± 0.579
3.946ValSer: 3.946 ± 0.478
4.41ValThr: 4.41 ± 0.613
5.106ValVal: 5.106 ± 0.922
0.928ValTrp: 0.928 ± 0.252
1.934ValTyr: 1.934 ± 0.484
0.0ValXaa: 0.0 ± 0.0
Trp
1.238TrpAla: 1.238 ± 0.269
0.077TrpCys: 0.077 ± 0.093
0.851TrpAsp: 0.851 ± 0.248
0.696TrpGlu: 0.696 ± 0.199
0.464TrpPhe: 0.464 ± 0.179
0.851TrpGly: 0.851 ± 0.194
0.696TrpHis: 0.696 ± 0.233
0.464TrpIle: 0.464 ± 0.19
1.006TrpLys: 1.006 ± 0.205
2.012TrpLeu: 2.012 ± 0.454
0.464TrpMet: 0.464 ± 0.166
0.542TrpAsn: 0.542 ± 0.217
0.232TrpPro: 0.232 ± 0.122
0.464TrpGln: 0.464 ± 0.238
0.696TrpArg: 0.696 ± 0.173
1.315TrpSer: 1.315 ± 0.359
1.161TrpThr: 1.161 ± 0.327
1.393TrpVal: 1.393 ± 0.31
0.309TrpTrp: 0.309 ± 0.155
0.309TrpTyr: 0.309 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.863TyrAla: 2.863 ± 0.444
0.464TyrCys: 0.464 ± 0.188
1.857TyrAsp: 1.857 ± 0.426
2.631TyrGlu: 2.631 ± 0.525
1.006TyrPhe: 1.006 ± 0.308
3.482TyrGly: 3.482 ± 0.529
0.851TyrHis: 0.851 ± 0.256
1.083TyrIle: 1.083 ± 0.318
2.321TyrLys: 2.321 ± 0.478
2.553TyrLeu: 2.553 ± 0.355
0.696TyrMet: 0.696 ± 0.179
1.315TyrAsn: 1.315 ± 0.304
1.006TyrPro: 1.006 ± 0.241
1.161TyrGln: 1.161 ± 0.287
2.012TyrArg: 2.012 ± 0.33
1.47TyrSer: 1.47 ± 0.376
1.393TyrThr: 1.393 ± 0.361
1.547TyrVal: 1.547 ± 0.297
0.309TyrTrp: 0.309 ± 0.166
0.464TyrTyr: 0.464 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12926 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski