Fowlpox virus (strain NVSL) (FPV)
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 251 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9J572|Q9J572_FOWPN ORF FPV159 N1R/p28 gene family protein OS=Fowlpox virus (strain NVSL) OX=928301 GN=FPV159 PE=4 SV=1
MM1 pKa = 7.4 IRR3 pKa = 11.84 IIILSLLFINVTTDD17 pKa = 3.0 SQEE20 pKa = 3.88 SSKK23 pKa = 11.12 NIQNVLHH30 pKa = 4.88 VTEE33 pKa = 4.43 YY34 pKa = 11.14 SRR36 pKa = 11.84 TGVTACSLHH45 pKa = 6.74 CFDD48 pKa = 5.25 RR49 pKa = 11.84 SKK51 pKa = 11.54 GLDD54 pKa = 3.29 QPKK57 pKa = 8.87 TFILPGKK64 pKa = 9.66 YY65 pKa = 9.99 SNNSIKK71 pKa = 10.87 LEE73 pKa = 4.12 VAIDD77 pKa = 3.92 TYY79 pKa = 11.58 KK80 pKa = 10.6 KK81 pKa = 10.7 DD82 pKa = 3.28 SDD84 pKa = 3.66 FSYY87 pKa = 10.74 SHH89 pKa = 7.57 PCQIFQFCVSGNFSGKK105 pKa = 10.17 RR106 pKa = 11.84 FDD108 pKa = 3.75 HH109 pKa = 6.1 YY110 pKa = 11.22 LYY112 pKa = 10.49 GYY114 pKa = 7.55 TISGFIDD121 pKa = 3.63 IAPKK125 pKa = 10.21 YY126 pKa = 8.37 YY127 pKa = 10.66 SGMSISTITVMPLQEE142 pKa = 4.73 GSLKK146 pKa = 10.54 HH147 pKa = 7.11 DD148 pKa = 4.52 DD149 pKa = 4.33 ADD151 pKa = 5.16 DD152 pKa = 3.68 YY153 pKa = 11.7 DD154 pKa = 5.13 YY155 pKa = 11.81 DD156 pKa = 4.79 DD157 pKa = 5.44 DD158 pKa = 4.15 CVPYY162 pKa = 10.82 KK163 pKa = 9.49 EE164 pKa = 4.43 TQPRR168 pKa = 11.84 HH169 pKa = 4.94 MPEE172 pKa = 3.82 SVIKK176 pKa = 9.7 EE177 pKa = 4.11 GCKK180 pKa = 9.99 PIPLPRR186 pKa = 11.84 YY187 pKa = 9.74 DD188 pKa = 4.68 EE189 pKa = 5.08 NDD191 pKa = 3.57 DD192 pKa = 4.01 PTCIMYY198 pKa = 9.47 WDD200 pKa = 4.67 HH201 pKa = 7.43 SWDD204 pKa = 3.52 NYY206 pKa = 9.9 CNVGFFNSLQSDD218 pKa = 4.07 HH219 pKa = 6.78 NPLVFPLTSYY229 pKa = 11.6 SDD231 pKa = 3.37 INNAFHH237 pKa = 7.41 AFQSSYY243 pKa = 10.74 CRR245 pKa = 11.84 SLGFNQSYY253 pKa = 9.06 SVCVSIGDD261 pKa = 3.77 TPFEE265 pKa = 4.35 VTYY268 pKa = 10.58 HH269 pKa = 6.23 SYY271 pKa = 11.43 EE272 pKa = 4.34 SVTVDD277 pKa = 4.52 QLLQEE282 pKa = 4.31 IKK284 pKa = 9.71 TLYY287 pKa = 11.06 GEE289 pKa = 4.31 DD290 pKa = 3.48 AVYY293 pKa = 10.79 GLPFRR298 pKa = 11.84 NITIMARR305 pKa = 11.84 TRR307 pKa = 11.84 IQSLPLTNNTCIPKK321 pKa = 9.81 QDD323 pKa = 4.4 DD324 pKa = 3.71 ADD326 pKa = 4.33 DD327 pKa = 4.28 VDD329 pKa = 5.3 DD330 pKa = 6.47 ADD332 pKa = 5.75 DD333 pKa = 5.13 VDD335 pKa = 5.16 DD336 pKa = 6.63 ADD338 pKa = 6.03 DD339 pKa = 5.77 ADD341 pKa = 5.77 DD342 pKa = 5.39 DD343 pKa = 5.13 DD344 pKa = 6.79 DD345 pKa = 5.74 YY346 pKa = 11.91 EE347 pKa = 5.48 LYY349 pKa = 10.97 VEE351 pKa = 4.42 TTPRR355 pKa = 11.84 VPTARR360 pKa = 11.84 KK361 pKa = 9.53 KK362 pKa = 10.48 PVTEE366 pKa = 4.25 EE367 pKa = 3.94 YY368 pKa = 11.27 NDD370 pKa = 3.43 IFSSFDD376 pKa = 3.26 NFDD379 pKa = 3.59 MKK381 pKa = 10.82 KK382 pKa = 10.03 KK383 pKa = 10.59
Molecular weight: 43.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.093
IPC2_protein 4.317
IPC_protein 4.342
Toseland 4.126
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.266
Rodwell 4.164
Grimsley 4.037
Solomon 4.329
Lehninger 4.291
Nozaki 4.444
DTASelect 4.724
Thurlkill 4.164
EMBOSS 4.279
Sillero 4.469
Patrickios 1.151
IPC_peptide 4.329
IPC2_peptide 4.444
IPC2.peptide.svr19 4.345
Protein with the highest isoelectric point:
>tr|Q9ICF5|Q9ICF5_FOWPN ORF FPV005 EFc gene family protein OS=Fowlpox virus (strain NVSL) OX=928301 GN=FPV005 PE=4 SV=1
MM1 pKa = 7.31 NNDD4 pKa = 3.74 TIFTLFYY11 pKa = 10.24 CKK13 pKa = 10.01 NKK15 pKa = 10.26 KK16 pKa = 8.03 YY17 pKa = 10.88 VRR19 pKa = 11.84 GEE21 pKa = 3.65 GGRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 GKK28 pKa = 8.62 TGILLFHH35 pKa = 7.38 PINHH39 pKa = 6.04 RR40 pKa = 11.84 VIGTSAHH47 pKa = 4.55 QCYY50 pKa = 7.68 KK51 pKa = 8.7 TRR53 pKa = 11.84 RR54 pKa = 11.84 IGFKK58 pKa = 10.18 LYY60 pKa = 10.64 AVAPRR65 pKa = 11.84 HH66 pKa = 5.64 VSTIRR71 pKa = 11.84 CGRR74 pKa = 11.84 SHH76 pKa = 6.53 SAHH79 pKa = 6.32 RR80 pKa = 11.84 VDD82 pKa = 4.51 KK83 pKa = 11.1 FSFSFQKK90 pKa = 10.53 VDD92 pKa = 4.42 FHH94 pKa = 7.29 CIAGSGAA101 pKa = 3.35
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.882
IPC_protein 10.657
Toseland 10.818
ProMoST 10.496
Dawson 10.906
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 11.199
Grimsley 10.95
Solomon 11.008
Lehninger 10.979
Nozaki 10.804
DTASelect 10.599
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.833
Patrickios 10.935
IPC_peptide 11.023
IPC2_peptide 9.794
IPC2.peptide.svr19 8.351
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
251
0
251
80735
60
1949
321.7
37.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.433 ± 0.097
2.2 ± 0.067
6.4 ± 0.107
5.599 ± 0.104
4.255 ± 0.118
4.003 ± 0.11
2.019 ± 0.064
10.035 ± 0.187
8.224 ± 0.125
9.109 ± 0.173
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.06
7.235 ± 0.148
3.151 ± 0.093
1.99 ± 0.069
4.132 ± 0.093
7.889 ± 0.164
5.724 ± 0.121
5.672 ± 0.097
0.666 ± 0.038
5.708 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here