Seal anellovirus 3
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5NF40|V5NF40_9VIRU Capsid protein OS=Seal anellovirus 3 OX=1427156 PE=3 SV=1
MM1 pKa = 7.49 MINHH5 pKa = 6.08 QPHH8 pKa = 7.59 AIQTTGDD15 pKa = 3.51 PFYY18 pKa = 10.27 HH19 pKa = 5.94 QKK21 pKa = 11.18 GLVLVSQSNMTQFLFGFAIASNLRR45 pKa = 11.84 SLEE48 pKa = 4.03 TLSTQEE54 pKa = 4.25 SPAGQQRR61 pKa = 11.84 RR62 pKa = 11.84 SSVKK66 pKa = 10.49 RR67 pKa = 11.84 QDD69 pKa = 3.96 LSQKK73 pKa = 10.0 YY74 pKa = 9.18 RR75 pKa = 11.84 LAPYY79 pKa = 9.92 SRR81 pKa = 11.84 PDD83 pKa = 3.41 LQTLGTYY90 pKa = 9.43 LQEE93 pKa = 4.24 TSTRR97 pKa = 11.84 TASSRR102 pKa = 11.84 MRR104 pKa = 11.84 VLNEE108 pKa = 3.78 LLDD111 pKa = 5.03 LIQEE115 pKa = 4.41 DD116 pKa = 4.84 SQPEE120 pKa = 4.03 WKK122 pKa = 9.9 PIRR125 pKa = 11.84 TSSSKK130 pKa = 10.73 DD131 pKa = 3.19 VSDD134 pKa = 4.76 FDD136 pKa = 3.98 TTPGSEE142 pKa = 4.36 SEE144 pKa = 4.56 DD145 pKa = 3.55 SDD147 pKa = 4.73 VSYY150 pKa = 11.4 LDD152 pKa = 3.77 WDD154 pKa = 3.94 WEE156 pKa = 4.29 DD157 pKa = 3.75 EE158 pKa = 4.32 PNPGGKK164 pKa = 9.88 GPPLVPPTPGPKK176 pKa = 9.34 AQGYY180 pKa = 7.22 PPHH183 pKa = 6.79 FLFF186 pKa = 5.84
Molecular weight: 20.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.087
IPC2_protein 5.118
IPC_protein 5.029
Toseland 4.991
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.169
Wikipedia 4.94
Rodwell 4.952
Grimsley 4.927
Solomon 5.041
Lehninger 5.003
Nozaki 5.181
DTASelect 5.359
Thurlkill 5.003
EMBOSS 5.003
Sillero 5.245
Patrickios 4.202
IPC_peptide 5.054
IPC2_peptide 5.232
IPC2.peptide.svr19 5.244
Protein with the highest isoelectric point:
>tr|V5NGP8|V5NGP8_9VIRU ORF2 OS=Seal anellovirus 3 OX=1427156 PE=4 SV=1
MM1 pKa = 8.06 AYY3 pKa = 9.86 RR4 pKa = 11.84 HH5 pKa = 5.89 RR6 pKa = 11.84 RR7 pKa = 11.84 PFRR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 7.7 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 8.37 RR17 pKa = 11.84 RR18 pKa = 11.84 WRR20 pKa = 11.84 GRR22 pKa = 11.84 KK23 pKa = 6.34 VHH25 pKa = 6.59 HH26 pKa = 6.49 RR27 pKa = 11.84 RR28 pKa = 11.84 TWRR31 pKa = 11.84 PFHH34 pKa = 6.47 RR35 pKa = 11.84 FGWRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 WRR43 pKa = 11.84 HH44 pKa = 4.77 PRR46 pKa = 11.84 RR47 pKa = 11.84 GSAVVRR53 pKa = 11.84 ITRR56 pKa = 11.84 PKK58 pKa = 7.13 KK59 pKa = 9.24 HH60 pKa = 6.56 KK61 pKa = 10.35 YY62 pKa = 7.45 ITVTGWEE69 pKa = 4.22 PLGNCCVTDD78 pKa = 3.65 SAGAEE83 pKa = 3.85 AKK85 pKa = 10.23 PYY87 pKa = 10.74 EE88 pKa = 4.77 DD89 pKa = 4.46 LDD91 pKa = 4.63 KK92 pKa = 11.43 DD93 pKa = 3.82 VATWCTQNNNAVWHH107 pKa = 5.67 GTFGHH112 pKa = 7.13 HH113 pKa = 5.93 YY114 pKa = 8.2 LTWGLLVQRR123 pKa = 11.84 AQYY126 pKa = 8.59 YY127 pKa = 8.59 FCKK130 pKa = 10.42 FSSDD134 pKa = 3.45 YY135 pKa = 10.79 RR136 pKa = 11.84 GYY138 pKa = 11.09 DD139 pKa = 3.35 YY140 pKa = 11.57 LSFCGGYY147 pKa = 8.81 IWLPYY152 pKa = 10.29 LYY154 pKa = 10.0 APYY157 pKa = 9.8 MFWLDD162 pKa = 3.38 PCIIDD167 pKa = 3.96 PQEE170 pKa = 4.26 KK171 pKa = 10.39 LMNFQLYY178 pKa = 10.21 KK179 pKa = 10.23 KK180 pKa = 9.74 DD181 pKa = 3.61 SSWFHH186 pKa = 6.48 PGIFMNRR193 pKa = 11.84 KK194 pKa = 7.12 GARR197 pKa = 11.84 FIPPSYY203 pKa = 9.1 QTYY206 pKa = 7.55 THH208 pKa = 6.82 HH209 pKa = 6.66 LYY211 pKa = 10.53 KK212 pKa = 10.45 RR213 pKa = 11.84 LRR215 pKa = 11.84 VPRR218 pKa = 11.84 PPTWEE223 pKa = 3.83 GVYY226 pKa = 9.12 TIPAATQYY234 pKa = 11.32 ILTHH238 pKa = 5.73 WAWTTVTTNTAFFDD252 pKa = 4.51 GACNQSGTTNTCEE265 pKa = 3.61 AVPWFMKK272 pKa = 9.86 GHH274 pKa = 5.23 QPAGKK279 pKa = 9.33 FAQKK283 pKa = 8.29 LTKK286 pKa = 9.56 WKK288 pKa = 9.25 TDD290 pKa = 3.26 TADD293 pKa = 3.15 GTKK296 pKa = 10.66 NLDD299 pKa = 3.5 CFGDD303 pKa = 3.77 PRR305 pKa = 11.84 SWWVNRR311 pKa = 11.84 RR312 pKa = 11.84 KK313 pKa = 10.2 YY314 pKa = 10.56 DD315 pKa = 3.82 DD316 pKa = 4.44 KK317 pKa = 11.83 SSTTCNTDD325 pKa = 1.96 NWGPFLPSKK334 pKa = 10.18 GTGTGVTKK342 pKa = 10.54 QYY344 pKa = 11.39 DD345 pKa = 3.89 SISFWFRR352 pKa = 11.84 YY353 pKa = 8.89 RR354 pKa = 11.84 FKK356 pKa = 10.94 FKK358 pKa = 11.06 VSGDD362 pKa = 3.81 SIYY365 pKa = 10.97 TRR367 pKa = 11.84 IPSRR371 pKa = 11.84 TATEE375 pKa = 4.57 VISEE379 pKa = 4.27 APGPQSKK386 pKa = 9.51 IQARR390 pKa = 11.84 SILKK394 pKa = 8.11 TRR396 pKa = 11.84 PPDD399 pKa = 4.13 LGDD402 pKa = 3.54 ILAGDD407 pKa = 4.21 LDD409 pKa = 4.34 EE410 pKa = 7.64 DD411 pKa = 5.42 GILTDD416 pKa = 4.05 EE417 pKa = 4.1 SLEE420 pKa = 4.24 RR421 pKa = 11.84 IIRR424 pKa = 11.84 SHH426 pKa = 7.78 PGRR429 pKa = 11.84 QPAGVEE435 pKa = 3.88 ADD437 pKa = 3.57 PDD439 pKa = 3.5 QLKK442 pKa = 9.83 QRR444 pKa = 11.84 RR445 pKa = 11.84 VRR447 pKa = 11.84 LRR449 pKa = 11.84 HH450 pKa = 5.9 DD451 pKa = 3.57 PGVRR455 pKa = 11.84 KK456 pKa = 9.59 RR457 pKa = 11.84 RR458 pKa = 11.84 LRR460 pKa = 11.84 RR461 pKa = 11.84 LLSGLGLGRR470 pKa = 11.84 RR471 pKa = 11.84 TQSGGEE477 pKa = 4.26 GPPPSPPHH485 pKa = 5.82 PWAA488 pKa = 4.64
Molecular weight: 57.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.604
IPC_protein 10.072
Toseland 10.175
ProMoST 9.94
Dawson 10.394
Bjellqvist 10.116
Wikipedia 10.599
Rodwell 10.657
Grimsley 10.482
Solomon 10.423
Lehninger 10.379
Nozaki 10.204
DTASelect 10.101
Thurlkill 10.233
EMBOSS 10.584
Sillero 10.306
Patrickios 10.087
IPC_peptide 10.423
IPC2_peptide 9.107
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
757
83
488
252.3
29.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.756 ± 0.537
1.585 ± 0.615
6.077 ± 0.367
3.303 ± 0.805
4.227 ± 0.264
7.398 ± 0.614
2.906 ± 0.556
4.359 ± 1.414
5.02 ± 0.674
7.53 ± 1.301
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.453 ± 0.402
2.906 ± 0.412
7.926 ± 0.713
4.756 ± 1.346
8.983 ± 1.954
7.001 ± 1.905
7.53 ± 0.609
4.227 ± 0.644
3.435 ± 0.836
4.624 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here