Gordonia phage Daredevil

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Daredevilvirus; Gordonia virus DareDevil

Average proteome isoelectric point is 5.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MJ14|A0A345MJ14_9CAUD Uncharacterized protein OS=Gordonia phage Daredevil OX=2283286 GN=159 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 5.1NPRR5 pKa = 11.84IWIGDD10 pKa = 3.77LAAYY14 pKa = 9.27NAGEE18 pKa = 5.38LVGQWIEE25 pKa = 4.05LDD27 pKa = 3.62STTTEE32 pKa = 4.06GDD34 pKa = 3.2IEE36 pKa = 4.16DD37 pKa = 5.22AIAEE41 pKa = 4.08ILEE44 pKa = 4.59LGTAKK49 pKa = 10.62LKK51 pKa = 10.98AQGEE55 pKa = 4.23WSGPHH60 pKa = 5.87EE61 pKa = 4.47EE62 pKa = 4.27WLIADD67 pKa = 4.45TDD69 pKa = 3.99CFGPIKK75 pKa = 10.11IGEE78 pKa = 4.18YY79 pKa = 9.28EE80 pKa = 4.28QISEE84 pKa = 4.08ILAHH88 pKa = 5.36VEE90 pKa = 4.62RR91 pKa = 11.84MGDD94 pKa = 3.73DD95 pKa = 3.36PDD97 pKa = 4.7KK98 pKa = 10.05YY99 pKa = 9.46FAYY102 pKa = 10.23VEE104 pKa = 4.41HH105 pKa = 7.0HH106 pKa = 6.16GSMYY110 pKa = 10.96ADD112 pKa = 5.56GYY114 pKa = 8.21TQDD117 pKa = 4.22SVIGPLGEE125 pKa = 4.19TMLEE129 pKa = 3.82VAYY132 pKa = 10.54ALVDD136 pKa = 3.71DD137 pKa = 4.25YY138 pKa = 11.65GYY140 pKa = 11.36LEE142 pKa = 4.46GVPEE146 pKa = 3.94HH147 pKa = 5.62VARR150 pKa = 11.84YY151 pKa = 8.97FDD153 pKa = 3.93YY154 pKa = 11.07EE155 pKa = 3.93AFARR159 pKa = 11.84DD160 pKa = 3.79LRR162 pKa = 11.84IEE164 pKa = 4.35GNFTEE169 pKa = 4.52YY170 pKa = 10.7DD171 pKa = 3.57GQFYY175 pKa = 10.42EE176 pKa = 4.32ITGDD180 pKa = 3.31

Molecular weight: 20.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MIQ9|A0A345MIQ9_9CAUD Uncharacterized protein OS=Gordonia phage Daredevil OX=2283286 GN=53 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84SGKK5 pKa = 9.73GRR7 pKa = 11.84PCPTPSKK14 pKa = 8.98TVYY17 pKa = 10.24GSGYY21 pKa = 7.33EE22 pKa = 4.09AEE24 pKa = 3.96RR25 pKa = 11.84AMYY28 pKa = 10.44KK29 pKa = 8.3MWRR32 pKa = 11.84AGRR35 pKa = 11.84ARR37 pKa = 11.84RR38 pKa = 11.84GNKK41 pKa = 9.54LPSRR45 pKa = 11.84TYY47 pKa = 10.46RR48 pKa = 11.84CPCGKK53 pKa = 8.68WHH55 pKa = 6.6LTSKK59 pKa = 9.99PKK61 pKa = 9.81RR62 pKa = 11.84DD63 pKa = 2.68RR64 pKa = 11.84VQVNEE69 pKa = 4.03GNYY72 pKa = 9.16VRR74 pKa = 11.84QQ75 pKa = 3.51

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

165

0

165

27531

34

2936

166.9

18.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.002 ± 0.475

0.908 ± 0.141

7.468 ± 0.241

6.869 ± 0.288

2.775 ± 0.116

7.915 ± 0.314

2.118 ± 0.162

5.26 ± 0.135

3.948 ± 0.209

7.929 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.09

3.185 ± 0.18

4.747 ± 0.159

3.487 ± 0.226

6.876 ± 0.218

5.641 ± 0.185

6.08 ± 0.174

6.72 ± 0.202

1.9 ± 0.127

2.793 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski