Amino acid dipepetide frequency for Gordonia phage Daredevil

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.803AlaAla: 13.803 ± 1.084
0.654AlaCys: 0.654 ± 0.157
8.79AlaAsp: 8.79 ± 0.637
8.463AlaGlu: 8.463 ± 0.646
2.724AlaPhe: 2.724 ± 0.256
8.79AlaGly: 8.79 ± 0.631
1.889AlaHis: 1.889 ± 0.247
6.32AlaIle: 6.32 ± 0.489
5.703AlaLys: 5.703 ± 0.75
8.79AlaLeu: 8.79 ± 0.778
3.414AlaMet: 3.414 ± 0.353
3.814AlaAsn: 3.814 ± 0.497
3.996AlaPro: 3.996 ± 0.357
3.887AlaGln: 3.887 ± 0.707
7.337AlaArg: 7.337 ± 0.541
4.831AlaSer: 4.831 ± 0.376
5.667AlaThr: 5.667 ± 0.654
6.865AlaVal: 6.865 ± 0.496
1.961AlaTrp: 1.961 ± 0.242
2.325AlaTyr: 2.325 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
1.09CysAla: 1.09 ± 0.25
0.218CysCys: 0.218 ± 0.106
0.835CysAsp: 0.835 ± 0.222
0.472CysGlu: 0.472 ± 0.179
0.254CysPhe: 0.254 ± 0.102
0.944CysGly: 0.944 ± 0.201
0.291CysHis: 0.291 ± 0.117
0.436CysIle: 0.436 ± 0.14
0.327CysLys: 0.327 ± 0.133
0.545CysLeu: 0.545 ± 0.152
0.218CysMet: 0.218 ± 0.093
0.363CysAsn: 0.363 ± 0.096
0.726CysPro: 0.726 ± 0.201
0.254CysGln: 0.254 ± 0.097
0.763CysArg: 0.763 ± 0.215
0.254CysSer: 0.254 ± 0.108
0.291CysThr: 0.291 ± 0.107
0.436CysVal: 0.436 ± 0.141
0.109CysTrp: 0.109 ± 0.07
0.254CysTyr: 0.254 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
6.902AspAla: 6.902 ± 0.648
0.763AspCys: 0.763 ± 0.182
5.158AspAsp: 5.158 ± 0.558
6.066AspGlu: 6.066 ± 0.524
2.288AspPhe: 2.288 ± 0.306
6.865AspGly: 6.865 ± 0.499
1.671AspHis: 1.671 ± 0.234
3.814AspIle: 3.814 ± 0.379
2.252AspLys: 2.252 ± 0.357
6.902AspLeu: 6.902 ± 0.491
1.598AspMet: 1.598 ± 0.23
2.143AspAsn: 2.143 ± 0.445
3.923AspPro: 3.923 ± 0.505
2.216AspGln: 2.216 ± 0.273
4.323AspArg: 4.323 ± 0.388
3.414AspSer: 3.414 ± 0.345
4.286AspThr: 4.286 ± 0.352
5.667AspVal: 5.667 ± 0.478
1.925AspTrp: 1.925 ± 0.335
2.506AspTyr: 2.506 ± 0.343
0.0AspXaa: 0.0 ± 0.0
Glu
7.446GluAla: 7.446 ± 0.64
0.872GluCys: 0.872 ± 0.228
5.122GluAsp: 5.122 ± 0.426
4.068GluGlu: 4.068 ± 0.41
2.325GluPhe: 2.325 ± 0.339
4.758GluGly: 4.758 ± 0.489
1.38GluHis: 1.38 ± 0.308
4.722GluIle: 4.722 ± 0.468
2.179GluLys: 2.179 ± 0.385
6.393GluLeu: 6.393 ± 0.609
1.744GluMet: 1.744 ± 0.247
2.07GluAsn: 2.07 ± 0.271
2.688GluPro: 2.688 ± 0.334
2.579GluGln: 2.579 ± 0.298
4.758GluArg: 4.758 ± 0.472
3.596GluSer: 3.596 ± 0.421
3.378GluThr: 3.378 ± 0.281
4.649GluVal: 4.649 ± 0.474
1.635GluTrp: 1.635 ± 0.248
2.325GluTyr: 2.325 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
2.833PheAla: 2.833 ± 0.326
0.4PheCys: 0.4 ± 0.12
3.088PheAsp: 3.088 ± 0.345
1.707PheGlu: 1.707 ± 0.256
0.545PhePhe: 0.545 ± 0.154
2.434PheGly: 2.434 ± 0.243
0.581PheHis: 0.581 ± 0.171
0.835PheIle: 0.835 ± 0.172
1.126PheLys: 1.126 ± 0.172
1.38PheLeu: 1.38 ± 0.253
0.835PheMet: 0.835 ± 0.2
1.235PheAsn: 1.235 ± 0.195
1.053PhePro: 1.053 ± 0.194
0.944PheGln: 0.944 ± 0.171
2.143PheArg: 2.143 ± 0.263
1.598PheSer: 1.598 ± 0.23
2.179PheThr: 2.179 ± 0.291
1.489PheVal: 1.489 ± 0.292
0.69PheTrp: 0.69 ± 0.18
0.654PheTyr: 0.654 ± 0.157
0.0PheXaa: 0.0 ± 0.0
Gly
7.083GlyAla: 7.083 ± 0.689
0.763GlyCys: 0.763 ± 0.195
6.357GlyAsp: 6.357 ± 0.555
5.485GlyGlu: 5.485 ± 0.484
2.361GlyPhe: 2.361 ± 0.3
7.156GlyGly: 7.156 ± 0.955
1.707GlyHis: 1.707 ± 0.295
3.814GlyIle: 3.814 ± 0.535
3.305GlyLys: 3.305 ± 0.418
6.066GlyLeu: 6.066 ± 0.551
1.816GlyMet: 1.816 ± 0.296
2.688GlyAsn: 2.688 ± 0.427
3.16GlyPro: 3.16 ± 0.33
3.596GlyGln: 3.596 ± 0.527
4.94GlyArg: 4.94 ± 0.473
4.795GlySer: 4.795 ± 0.442
5.013GlyThr: 5.013 ± 0.495
4.758GlyVal: 4.758 ± 0.431
1.526GlyTrp: 1.526 ± 0.263
2.761GlyTyr: 2.761 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
1.562HisAla: 1.562 ± 0.301
0.218HisCys: 0.218 ± 0.116
1.308HisAsp: 1.308 ± 0.231
1.526HisGlu: 1.526 ± 0.239
0.799HisPhe: 0.799 ± 0.174
1.853HisGly: 1.853 ± 0.248
0.581HisHis: 0.581 ± 0.149
1.235HisIle: 1.235 ± 0.21
0.618HisLys: 0.618 ± 0.162
2.07HisLeu: 2.07 ± 0.266
0.545HisMet: 0.545 ± 0.126
0.69HisAsn: 0.69 ± 0.149
1.707HisPro: 1.707 ± 0.302
0.545HisGln: 0.545 ± 0.142
1.344HisArg: 1.344 ± 0.278
0.981HisSer: 0.981 ± 0.223
1.308HisThr: 1.308 ± 0.278
1.453HisVal: 1.453 ± 0.24
0.363HisTrp: 0.363 ± 0.095
0.472HisTyr: 0.472 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
5.848IleAla: 5.848 ± 0.44
0.509IleCys: 0.509 ± 0.153
4.504IleAsp: 4.504 ± 0.292
4.831IleGlu: 4.831 ± 0.449
1.162IlePhe: 1.162 ± 0.156
4.032IleGly: 4.032 ± 0.537
1.126IleHis: 1.126 ± 0.22
2.325IleIle: 2.325 ± 0.332
1.889IleLys: 1.889 ± 0.288
3.16IleLeu: 3.16 ± 0.311
0.908IleMet: 0.908 ± 0.161
1.925IleAsn: 1.925 ± 0.305
2.688IlePro: 2.688 ± 0.262
2.216IleGln: 2.216 ± 0.383
3.887IleArg: 3.887 ± 0.332
2.688IleSer: 2.688 ± 0.381
3.16IleThr: 3.16 ± 0.298
3.305IleVal: 3.305 ± 0.441
0.908IleTrp: 0.908 ± 0.208
1.526IleTyr: 1.526 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
4.577LysAla: 4.577 ± 0.597
0.109LysCys: 0.109 ± 0.075
2.143LysAsp: 2.143 ± 0.23
1.744LysGlu: 1.744 ± 0.256
1.199LysPhe: 1.199 ± 0.227
2.615LysGly: 2.615 ± 0.332
0.654LysHis: 0.654 ± 0.143
2.179LysIle: 2.179 ± 0.26
1.889LysLys: 1.889 ± 0.342
3.56LysLeu: 3.56 ± 0.428
1.053LysMet: 1.053 ± 0.177
0.835LysAsn: 0.835 ± 0.148
1.998LysPro: 1.998 ± 0.291
1.308LysGln: 1.308 ± 0.232
3.632LysArg: 3.632 ± 0.442
2.107LysSer: 2.107 ± 0.314
2.07LysThr: 2.07 ± 0.258
3.741LysVal: 3.741 ± 0.369
0.799LysTrp: 0.799 ± 0.175
1.271LysTyr: 1.271 ± 0.206
0.0LysXaa: 0.0 ± 0.0
Leu
9.117LeuAla: 9.117 ± 0.667
0.763LeuCys: 0.763 ± 0.171
6.829LeuAsp: 6.829 ± 0.472
5.449LeuGlu: 5.449 ± 0.55
1.925LeuPhe: 1.925 ± 0.273
6.03LeuGly: 6.03 ± 0.513
1.671LeuHis: 1.671 ± 0.272
4.541LeuIle: 4.541 ± 0.364
2.47LeuLys: 2.47 ± 0.287
6.175LeuLeu: 6.175 ± 0.545
1.235LeuMet: 1.235 ± 0.231
2.979LeuAsn: 2.979 ± 0.342
3.923LeuPro: 3.923 ± 0.359
2.761LeuGln: 2.761 ± 0.286
5.34LeuArg: 5.34 ± 0.499
4.758LeuSer: 4.758 ± 0.512
5.158LeuThr: 5.158 ± 0.427
4.94LeuVal: 4.94 ± 0.435
1.09LeuTrp: 1.09 ± 0.226
2.143LeuTyr: 2.143 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.906MetAla: 2.906 ± 0.311
0.327MetCys: 0.327 ± 0.109
0.726MetAsp: 0.726 ± 0.175
1.38MetGlu: 1.38 ± 0.202
0.545MetPhe: 0.545 ± 0.136
1.453MetGly: 1.453 ± 0.228
0.618MetHis: 0.618 ± 0.163
1.126MetIle: 1.126 ± 0.225
1.017MetLys: 1.017 ± 0.187
1.78MetLeu: 1.78 ± 0.225
0.363MetMet: 0.363 ± 0.125
0.726MetAsn: 0.726 ± 0.18
1.38MetPro: 1.38 ± 0.269
0.581MetGln: 0.581 ± 0.156
1.889MetArg: 1.889 ± 0.328
2.615MetSer: 2.615 ± 0.298
2.107MetThr: 2.107 ± 0.345
1.635MetVal: 1.635 ± 0.253
0.218MetTrp: 0.218 ± 0.086
0.4MetTyr: 0.4 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
4.068AsnAla: 4.068 ± 0.479
0.254AsnCys: 0.254 ± 0.099
1.78AsnAsp: 1.78 ± 0.213
1.598AsnGlu: 1.598 ± 0.219
0.799AsnPhe: 0.799 ± 0.2
3.088AsnGly: 3.088 ± 0.397
0.763AsnHis: 0.763 ± 0.156
1.308AsnIle: 1.308 ± 0.286
1.38AsnLys: 1.38 ± 0.248
2.47AsnLeu: 2.47 ± 0.35
0.581AsnMet: 0.581 ± 0.125
0.835AsnAsn: 0.835 ± 0.23
2.252AsnPro: 2.252 ± 0.273
1.199AsnGln: 1.199 ± 0.241
2.797AsnArg: 2.797 ± 0.305
2.034AsnSer: 2.034 ± 0.385
1.453AsnThr: 1.453 ± 0.225
1.816AsnVal: 1.816 ± 0.254
0.618AsnTrp: 0.618 ± 0.238
0.763AsnTyr: 0.763 ± 0.144
0.0AsnXaa: 0.0 ± 0.0
Pro
5.921ProAla: 5.921 ± 0.524
0.472ProCys: 0.472 ± 0.17
3.596ProAsp: 3.596 ± 0.405
3.741ProGlu: 3.741 ± 0.478
1.053ProPhe: 1.053 ± 0.197
4.286ProGly: 4.286 ± 0.431
1.017ProHis: 1.017 ± 0.182
2.107ProIle: 2.107 ± 0.256
1.961ProLys: 1.961 ± 0.267
2.87ProLeu: 2.87 ± 0.313
1.09ProMet: 1.09 ± 0.168
1.707ProAsn: 1.707 ± 0.233
2.543ProPro: 2.543 ± 0.383
1.162ProGln: 1.162 ± 0.194
2.07ProArg: 2.07 ± 0.323
3.051ProSer: 3.051 ± 0.348
3.342ProThr: 3.342 ± 0.426
3.233ProVal: 3.233 ± 0.33
0.944ProTrp: 0.944 ± 0.177
1.308ProTyr: 1.308 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
4.359GlnAla: 4.359 ± 0.723
0.254GlnCys: 0.254 ± 0.096
1.998GlnAsp: 1.998 ± 0.272
1.526GlnGlu: 1.526 ± 0.287
0.981GlnPhe: 0.981 ± 0.164
2.397GlnGly: 2.397 ± 0.34
0.69GlnHis: 0.69 ± 0.186
1.925GlnIle: 1.925 ± 0.275
1.453GlnLys: 1.453 ± 0.326
3.523GlnLeu: 3.523 ± 0.368
0.981GlnMet: 0.981 ± 0.216
0.799GlnAsn: 0.799 ± 0.156
1.816GlnPro: 1.816 ± 0.244
1.562GlnGln: 1.562 ± 0.371
2.543GlnArg: 2.543 ± 0.377
2.07GlnSer: 2.07 ± 0.23
1.453GlnThr: 1.453 ± 0.229
2.797GlnVal: 2.797 ± 0.278
0.763GlnTrp: 0.763 ± 0.171
0.981GlnTyr: 0.981 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
7.301ArgAla: 7.301 ± 0.701
0.545ArgCys: 0.545 ± 0.161
4.432ArgAsp: 4.432 ± 0.438
4.904ArgGlu: 4.904 ± 0.465
1.889ArgPhe: 1.889 ± 0.241
4.722ArgGly: 4.722 ± 0.393
1.671ArgHis: 1.671 ± 0.291
3.56ArgIle: 3.56 ± 0.37
3.088ArgLys: 3.088 ± 0.433
5.812ArgLeu: 5.812 ± 0.5
1.853ArgMet: 1.853 ± 0.279
2.434ArgAsn: 2.434 ± 0.237
3.16ArgPro: 3.16 ± 0.428
2.87ArgGln: 2.87 ± 0.312
5.558ArgArg: 5.558 ± 0.603
3.632ArgSer: 3.632 ± 0.445
4.504ArgThr: 4.504 ± 0.45
3.887ArgVal: 3.887 ± 0.474
1.235ArgTrp: 1.235 ± 0.234
1.671ArgTyr: 1.671 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
6.066SerAla: 6.066 ± 0.413
0.436SerCys: 0.436 ± 0.186
4.177SerAsp: 4.177 ± 0.377
3.378SerGlu: 3.378 ± 0.348
1.744SerPhe: 1.744 ± 0.241
4.323SerGly: 4.323 ± 0.534
1.162SerHis: 1.162 ± 0.219
2.579SerIle: 2.579 ± 0.326
2.07SerLys: 2.07 ± 0.257
4.649SerLeu: 4.649 ± 0.448
1.78SerMet: 1.78 ± 0.255
1.38SerAsn: 1.38 ± 0.263
2.833SerPro: 2.833 ± 0.245
1.744SerGln: 1.744 ± 0.284
4.177SerArg: 4.177 ± 0.545
2.979SerSer: 2.979 ± 0.356
4.177SerThr: 4.177 ± 0.352
4.323SerVal: 4.323 ± 0.463
1.053SerTrp: 1.053 ± 0.208
1.38SerTyr: 1.38 ± 0.196
0.0SerXaa: 0.0 ± 0.0
Thr
6.393ThrAla: 6.393 ± 0.466
0.472ThrCys: 0.472 ± 0.138
4.286ThrAsp: 4.286 ± 0.354
3.887ThrGlu: 3.887 ± 0.39
2.034ThrPhe: 2.034 ± 0.241
4.867ThrGly: 4.867 ± 0.464
1.199ThrHis: 1.199 ± 0.208
3.088ThrIle: 3.088 ± 0.35
2.906ThrLys: 2.906 ± 0.345
4.577ThrLeu: 4.577 ± 0.431
1.344ThrMet: 1.344 ± 0.21
1.635ThrAsn: 1.635 ± 0.267
3.378ThrPro: 3.378 ± 0.381
1.489ThrGln: 1.489 ± 0.272
3.451ThrArg: 3.451 ± 0.397
4.432ThrSer: 4.432 ± 0.479
3.923ThrThr: 3.923 ± 0.383
4.359ThrVal: 4.359 ± 0.485
1.199ThrTrp: 1.199 ± 0.257
1.889ThrTyr: 1.889 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
8.137ValAla: 8.137 ± 0.478
0.545ValCys: 0.545 ± 0.157
5.884ValAsp: 5.884 ± 0.487
5.449ValGlu: 5.449 ± 0.467
1.889ValPhe: 1.889 ± 0.256
4.867ValGly: 4.867 ± 0.477
1.344ValHis: 1.344 ± 0.24
3.959ValIle: 3.959 ± 0.373
2.579ValLys: 2.579 ± 0.307
4.686ValLeu: 4.686 ± 0.459
1.308ValMet: 1.308 ± 0.22
2.034ValAsn: 2.034 ± 0.277
2.833ValPro: 2.833 ± 0.348
2.07ValGln: 2.07 ± 0.25
4.286ValArg: 4.286 ± 0.385
3.233ValSer: 3.233 ± 0.36
4.214ValThr: 4.214 ± 0.401
4.795ValVal: 4.795 ± 0.465
1.09ValTrp: 1.09 ± 0.217
1.998ValTyr: 1.998 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
2.107TrpAla: 2.107 ± 0.243
0.291TrpCys: 0.291 ± 0.127
1.707TrpAsp: 1.707 ± 0.198
0.981TrpGlu: 0.981 ± 0.248
0.472TrpPhe: 0.472 ± 0.121
1.271TrpGly: 1.271 ± 0.256
0.436TrpHis: 0.436 ± 0.139
1.271TrpIle: 1.271 ± 0.231
0.291TrpLys: 0.291 ± 0.097
1.744TrpLeu: 1.744 ± 0.286
0.472TrpMet: 0.472 ± 0.135
0.581TrpAsn: 0.581 ± 0.124
0.4TrpPro: 0.4 ± 0.125
0.835TrpGln: 0.835 ± 0.136
1.417TrpArg: 1.417 ± 0.25
1.635TrpSer: 1.635 ± 0.34
1.199TrpThr: 1.199 ± 0.21
1.053TrpVal: 1.053 ± 0.216
0.327TrpTrp: 0.327 ± 0.119
0.509TrpTyr: 0.509 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.506TyrAla: 2.506 ± 0.316
0.218TyrCys: 0.218 ± 0.108
1.961TyrAsp: 1.961 ± 0.264
2.034TyrGlu: 2.034 ± 0.349
0.763TyrPhe: 0.763 ± 0.179
2.397TyrGly: 2.397 ± 0.33
0.726TyrHis: 0.726 ± 0.203
1.453TyrIle: 1.453 ± 0.249
0.981TyrLys: 0.981 ± 0.125
2.143TyrLeu: 2.143 ± 0.271
0.618TyrMet: 0.618 ± 0.172
0.981TyrAsn: 0.981 ± 0.168
1.017TyrPro: 1.017 ± 0.223
1.053TyrGln: 1.053 ± 0.167
2.107TyrArg: 2.107 ± 0.265
1.707TyrSer: 1.707 ± 0.279
1.925TyrThr: 1.925 ± 0.377
1.998TyrVal: 1.998 ± 0.249
0.545TyrTrp: 0.545 ± 0.16
0.799TyrTyr: 0.799 ± 0.186
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 165 proteins (27531 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski