Pelotomaculum propionicicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Pelotomaculum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7RJG5|A0A4Y7RJG5_9FIRM Uncharacterized protein OS=Pelotomaculum propionicicum OX=258475 GN=Pmgp_03562 PE=4 SV=1
MM1 pKa = 7.12EE2 pKa = 5.82AKK4 pKa = 9.79ISHH7 pKa = 7.26DD8 pKa = 3.35IIFFKK13 pKa = 10.99GVAKK17 pKa = 10.95LNDD20 pKa = 3.96LKK22 pKa = 10.63TKK24 pKa = 9.95VAYY27 pKa = 10.06LQGLSAGLDD36 pKa = 3.59FNADD40 pKa = 3.44SKK42 pKa = 11.19EE43 pKa = 3.87GKK45 pKa = 9.04LLKK48 pKa = 10.77GIIEE52 pKa = 4.31VLDD55 pKa = 3.8EE56 pKa = 4.48FADD59 pKa = 3.88SVVDD63 pKa = 4.13LEE65 pKa = 4.37EE66 pKa = 4.43AQEE69 pKa = 3.98QMEE72 pKa = 5.22DD73 pKa = 3.6YY74 pKa = 10.94LEE76 pKa = 5.54SIDD79 pKa = 3.77EE80 pKa = 4.35DD81 pKa = 4.42LYY83 pKa = 10.94HH84 pKa = 8.24LEE86 pKa = 4.34DD87 pKa = 3.46EE88 pKa = 4.86VYY90 pKa = 10.89EE91 pKa = 4.09EE92 pKa = 5.89DD93 pKa = 5.25GLNTCADD100 pKa = 3.63HH101 pKa = 6.85YY102 pKa = 11.61LEE104 pKa = 4.76VDD106 pKa = 3.72CPRR109 pKa = 11.84CGEE112 pKa = 4.4TVCFDD117 pKa = 4.29SEE119 pKa = 4.16ILEE122 pKa = 4.85DD123 pKa = 4.25DD124 pKa = 5.21DD125 pKa = 4.67IVEE128 pKa = 4.57VTCPNCDD135 pKa = 2.5EE136 pKa = 4.37VVFVNDD142 pKa = 4.59GDD144 pKa = 4.07HH145 pKa = 6.0QAADD149 pKa = 3.81EE150 pKa = 4.43PEE152 pKa = 4.25ALVGKK157 pKa = 10.72VNDD160 pKa = 4.0TFGSEE165 pKa = 4.78DD166 pKa = 3.49EE167 pKa = 5.24DD168 pKa = 3.59II169 pKa = 5.1

Molecular weight: 18.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7RJH8|A0A4Y7RJH8_9FIRM Uncharacterized protein OS=Pelotomaculum propionicicum OX=258475 GN=Pmgp_03356 PE=4 SV=1
MM1 pKa = 7.42TEE3 pKa = 3.9YY4 pKa = 10.98NILGKK9 pKa = 9.51PIPRR13 pKa = 11.84LDD15 pKa = 3.55SRR17 pKa = 11.84SKK19 pKa = 8.94VTGEE23 pKa = 4.11TKK25 pKa = 10.36HH26 pKa = 6.97LNDD29 pKa = 2.88ITRR32 pKa = 11.84QRR34 pKa = 11.84RR35 pKa = 11.84SCRR38 pKa = 11.84GRR40 pKa = 11.84SCC42 pKa = 4.3

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3787

0

3787

1096290

29

1840

289.5

32.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.677 ± 0.045

1.288 ± 0.02

5.0 ± 0.026

6.838 ± 0.048

3.938 ± 0.03

7.88 ± 0.04

1.697 ± 0.017

6.791 ± 0.035

5.953 ± 0.034

10.168 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.017

3.847 ± 0.028

4.275 ± 0.027

3.286 ± 0.025

5.547 ± 0.039

5.614 ± 0.029

4.977 ± 0.029

7.443 ± 0.04

1.009 ± 0.016

3.255 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski