Chitinibacter sp. 2T18
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3281 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7H9BMK3|A0A7H9BMK3_9NEIS LysE family transporter OS=Chitinibacter sp. 2T18 OX=2739434 GN=HQ393_10320 PE=4 SV=1
MM1 pKa = 7.72 ASKK4 pKa = 7.71 TTQTASNSAKK14 pKa = 10.16 SANHH18 pKa = 6.06 TGVVTQVMGEE28 pKa = 4.3 VKK30 pKa = 10.54 VLTLAGEE37 pKa = 4.29 IKK39 pKa = 9.72 TLQVGDD45 pKa = 4.05 KK46 pKa = 10.73 VNVGDD51 pKa = 4.74 TIQTGSDD58 pKa = 3.44 GAVSISLDD66 pKa = 3.3 GGGTISVGRR75 pKa = 11.84 SDD77 pKa = 5.6 SMPLTNEE84 pKa = 3.32 MFAALVAPADD94 pKa = 3.77 NAAADD99 pKa = 3.56 AAKK102 pKa = 9.91 IQEE105 pKa = 5.07 LIAQGADD112 pKa = 3.36 PTQVAAASAAGAGGGEE128 pKa = 4.44 DD129 pKa = 3.56 GGHH132 pKa = 5.8 SFVVLDD138 pKa = 3.87 SPEE141 pKa = 3.86 SRR143 pKa = 11.84 VAVDD147 pKa = 3.09 TGFATTPVIQSVSLIPPDD165 pKa = 3.61 ASNAITPTNTPPVANNDD182 pKa = 3.45 TFTPASPPNALNDD195 pKa = 3.94 ALNTNEE201 pKa = 4.53 DD202 pKa = 4.06 TPLVIQGTTLLANDD216 pKa = 4.38 SDD218 pKa = 5.0 ADD220 pKa = 4.1 GNPLTIIGVGTAGNGTVILNADD242 pKa = 3.56 GSITYY247 pKa = 8.4 TPNANYY253 pKa = 10.2 NGPDD257 pKa = 3.15 SFTYY261 pKa = 9.94 TISDD265 pKa = 3.88 GQGGTSTATVTVGVTPVNDD284 pKa = 3.83 PPVLLNPEE292 pKa = 3.94 NLAPLGDD299 pKa = 4.3 DD300 pKa = 3.65 VSVTTPEE307 pKa = 3.93 DD308 pKa = 3.81 TPVGGKK314 pKa = 9.24 LAATDD319 pKa = 3.56 VDD321 pKa = 4.24 GDD323 pKa = 4.04 TLTFSLTGNQPAHH336 pKa = 5.73 GTVTVNADD344 pKa = 3.54 GTWLYY349 pKa = 10.23 TPNTNYY355 pKa = 10.53 NGPDD359 pKa = 3.26 SFTALVDD366 pKa = 4.0 DD367 pKa = 5.13 GKK369 pKa = 11.36 GGTDD373 pKa = 3.13 TLVVNIGVTPVNDD386 pKa = 3.64 PPVLVNPEE394 pKa = 3.72 NLAPLGDD401 pKa = 4.3 DD402 pKa = 3.65 VSVTTPEE409 pKa = 3.88 DD410 pKa = 3.72 TPIGGKK416 pKa = 9.37 LAATDD421 pKa = 3.9 ADD423 pKa = 4.09 GDD425 pKa = 4.14 TLTFSLTGNQPAHH438 pKa = 5.73 GTVTVNADD446 pKa = 3.54 GTWLYY451 pKa = 10.23 TPNTNYY457 pKa = 10.53 NGPDD461 pKa = 3.26 SFTAMVDD468 pKa = 3.47 DD469 pKa = 4.98 GKK471 pKa = 11.36 GGTDD475 pKa = 3.13 TLVVNIGVTPVNDD488 pKa = 3.65 PPVIVDD494 pKa = 4.16 PEE496 pKa = 4.08 NLAPLGDD503 pKa = 4.3 DD504 pKa = 3.65 VSVTTPEE511 pKa = 3.88 DD512 pKa = 3.72 TPIGGKK518 pKa = 9.37 LAATDD523 pKa = 3.86 ADD525 pKa = 4.11 GDD527 pKa = 4.3 SLTFSLTGNQPAHH540 pKa = 5.73 GTVTVNADD548 pKa = 3.54 GTWLYY553 pKa = 10.23 TPNTNYY559 pKa = 10.53 NGPDD563 pKa = 3.26 SFTAMVDD570 pKa = 3.47 DD571 pKa = 4.98 GKK573 pKa = 11.36 GGTDD577 pKa = 3.13 TLVVNIGVTPLNAPPTITTDD597 pKa = 3.06 NGNEE601 pKa = 4.18 GEE603 pKa = 4.6 GNDD606 pKa = 3.66 TVYY609 pKa = 11.04 EE610 pKa = 4.22 SGLATGTNAGVSTITASGTFTVADD634 pKa = 4.0 ADD636 pKa = 4.03 GLGDD640 pKa = 3.37 IHH642 pKa = 7.53 SVTVAGTTFVIGAGGLADD660 pKa = 4.44 LAALVGQTANTSHH673 pKa = 6.8 GSVLIDD679 pKa = 3.45 SYY681 pKa = 11.86 NNGVFGYY688 pKa = 8.5 TYY690 pKa = 10.31 TLTSPVTSGSAEE702 pKa = 3.57 SDD704 pKa = 3.57 SFNVSVADD712 pKa = 4.06 GSGASAPVTVTINIVDD728 pKa = 4.36 DD729 pKa = 4.79 APVSTSEE736 pKa = 4.21 HH737 pKa = 5.71 VSVTEE742 pKa = 3.95 DD743 pKa = 3.0 AASNVVSGNVLDD755 pKa = 4.58 NDD757 pKa = 3.62 VSGADD762 pKa = 3.62 SPKK765 pKa = 10.84 SFAGWSTGDD774 pKa = 3.39 AATITALQNFGTLVQDD790 pKa = 4.68 SDD792 pKa = 4.13 GHH794 pKa = 5.6 WSYY797 pKa = 10.13 TLNNGLSATQALTGSDD813 pKa = 3.02 QTDD816 pKa = 3.67 YY817 pKa = 11.13 VLHH820 pKa = 5.59 YY821 pKa = 8.91 TVQDD825 pKa = 3.43 ADD827 pKa = 4.01 GTTSPATLTITIKK840 pKa = 10.56 GANDD844 pKa = 3.85 GPTITTDD851 pKa = 2.95 NGNDD855 pKa = 3.82 GEE857 pKa = 4.78 GNDD860 pKa = 3.81 TVYY863 pKa = 11.04 EE864 pKa = 4.22 SGLATGTNAGVSTITASGTFTVADD888 pKa = 4.0 ADD890 pKa = 4.03 GLGDD894 pKa = 3.37 IHH896 pKa = 7.53 SVTVAGTTFVIGTGTGEE913 pKa = 4.26 FVDD916 pKa = 4.87 LAALVGQTANTSHH929 pKa = 6.8 GSVLIDD935 pKa = 3.35 SYY937 pKa = 12.14 NNGVYY942 pKa = 10.19 SYY944 pKa = 10.22 TYY946 pKa = 8.69 TLSGAVPDD954 pKa = 3.97 IANQVEE960 pKa = 4.49 TDD962 pKa = 3.42 SFTVSVSDD970 pKa = 3.95 GVAPAASATITIEE983 pKa = 3.9 INDD986 pKa = 5.47 DD987 pKa = 3.36 IPIAKK992 pKa = 9.76 ADD994 pKa = 3.96 SNNISANDD1002 pKa = 3.54 FNSEE1006 pKa = 4.06 FEE1008 pKa = 4.56 GVQEE1012 pKa = 4.21 LPAVQTSGNVLLGDD1026 pKa = 4.14 LNGGVADD1033 pKa = 4.05 VQGADD1038 pKa = 3.4 QPAKK1042 pKa = 7.84 VTQVIFGTNVQAVTTDD1058 pKa = 3.26 APTSIDD1064 pKa = 3.26 GKK1066 pKa = 11.13 YY1067 pKa = 8.8 GTLLLNSDD1075 pKa = 3.55 GSYY1078 pKa = 10.29 TYY1080 pKa = 10.6 TLNNSSNAVQEE1091 pKa = 4.41 LGNNEE1096 pKa = 4.15 HH1097 pKa = 6.52 PTDD1100 pKa = 3.6 VFSYY1104 pKa = 10.15 SIKK1107 pKa = 10.94 DD1108 pKa = 3.29 ADD1110 pKa = 3.81 GDD1112 pKa = 4.14 VSNLTSLTITVNGADD1127 pKa = 3.98 DD1128 pKa = 6.28 AIVLDD1133 pKa = 4.17 TKK1135 pKa = 11.26 LSGEE1139 pKa = 4.28 VSSGTVYY1146 pKa = 10.56 EE1147 pKa = 4.87 AGLSTGSKK1155 pKa = 10.56 ADD1157 pKa = 3.46 GDD1159 pKa = 4.1 ATTDD1163 pKa = 3.31 PTTFTSSFTIKK1174 pKa = 10.67 SIDD1177 pKa = 3.7 GLDD1180 pKa = 3.32 HH1181 pKa = 5.29 VTIGTGANTARR1192 pKa = 11.84 VNFTQAPDD1200 pKa = 4.19 GSWSASVADD1209 pKa = 4.24 SSADD1213 pKa = 3.42 SALGNSLTVTNAVYY1227 pKa = 10.74 SSTSHH1232 pKa = 5.48 TWTFSYY1238 pKa = 10.4 SYY1240 pKa = 9.65 TLNAPEE1246 pKa = 4.1 THH1248 pKa = 6.41 SAPGNDD1254 pKa = 3.28 NVSDD1258 pKa = 4.02 TLSVMALEE1266 pKa = 4.84 TDD1268 pKa = 3.53 GSTANGTLSITVVDD1282 pKa = 4.85 DD1283 pKa = 3.81 VPTAKK1288 pKa = 10.59 ADD1290 pKa = 3.75 FDD1292 pKa = 4.86 SVTEE1296 pKa = 4.41 DD1297 pKa = 3.21 ASSTIATGNVITGVDD1312 pKa = 3.58 LAGSHH1317 pKa = 7.34 DD1318 pKa = 4.57 SNSSDD1323 pKa = 3.66 GVADD1327 pKa = 3.52 VQGADD1332 pKa = 3.29 RR1333 pKa = 11.84 NVMVTHH1339 pKa = 7.01 IGFGTNVQEE1348 pKa = 4.4 VHH1350 pKa = 6.16 TGTPTSINGQYY1361 pKa = 11.16 GSLSLNSDD1369 pKa = 3.1 GSYY1372 pKa = 10.09 TYY1374 pKa = 10.94 SLHH1377 pKa = 6.2 NSSNAVQEE1385 pKa = 4.41 LGNNEE1390 pKa = 4.1 HH1391 pKa = 6.24 PTEE1394 pKa = 4.12 VFTYY1398 pKa = 9.5 TITDD1402 pKa = 3.44 ADD1404 pKa = 4.23 GDD1406 pKa = 4.04 RR1407 pKa = 11.84 SSQTLTISVNGADD1420 pKa = 5.29 DD1421 pKa = 6.6 AIALDD1426 pKa = 3.67 NKK1428 pKa = 10.7 LITEE1432 pKa = 4.5 TNSGTVNEE1440 pKa = 4.82 AGLPSGSLADD1450 pKa = 4.37 SDD1452 pKa = 4.33 PTTQPTSLAGSFTVKK1467 pKa = 10.61 SIDD1470 pKa = 3.67 GLDD1473 pKa = 3.31 HH1474 pKa = 5.29 VTIGTGAGNTATVSFTQAADD1494 pKa = 4.23 GSWTPHH1500 pKa = 5.22 FTDD1503 pKa = 4.94 PSAVSALGNSLSITGSSYY1521 pKa = 11.18 NAADD1525 pKa = 3.68 HH1526 pKa = 6.25 TWTLNYY1532 pKa = 10.16 TYY1534 pKa = 9.87 TLQAAEE1540 pKa = 4.16 THH1542 pKa = 5.97 SAAGNDD1548 pKa = 3.8 TLIDD1552 pKa = 3.78 TLAVSAIEE1560 pKa = 4.31 TDD1562 pKa = 3.24 GSTANGTIRR1571 pKa = 11.84 IGVVDD1576 pKa = 5.03 DD1577 pKa = 4.51 APIAKK1582 pKa = 9.98 DD1583 pKa = 3.19 ISLSSATHH1591 pKa = 4.51 VVEE1594 pKa = 4.39 TNLVLTIDD1602 pKa = 4.09 VSGSMQGSKK1611 pKa = 10.79 LDD1613 pKa = 3.84 LAKK1616 pKa = 10.44 QALSNVIDD1624 pKa = 3.83 QYY1626 pKa = 11.94 DD1627 pKa = 3.33 ALGDD1631 pKa = 3.66 VRR1633 pKa = 11.84 IMLVNFSSDD1642 pKa = 2.89 ASLQGSNTWLSIGAAKK1658 pKa = 10.54 ALINLLSAGGNTNFDD1673 pKa = 3.42 AALAQITTHH1682 pKa = 5.98 YY1683 pKa = 10.64 NDD1685 pKa = 3.33 YY1686 pKa = 11.38 GKK1688 pKa = 8.8 LTGEE1692 pKa = 4.5 GVQNVSYY1699 pKa = 9.95 FLSDD1703 pKa = 3.49 GVPTVGGGITGSEE1716 pKa = 3.66 IGAWTHH1722 pKa = 6.31 FVDD1725 pKa = 4.72 INDD1728 pKa = 3.64 IKK1730 pKa = 11.27 SLAFGIGTGATLSTLEE1746 pKa = 4.54 PIAYY1750 pKa = 9.99 DD1751 pKa = 3.51 GVTNTDD1757 pKa = 3.21 PSSGVTLVTNASNLPSALVNSAQPVLNFSLLDD1789 pKa = 3.56 NGGSFGADD1797 pKa = 2.42 GGHH1800 pKa = 6.2 VNNIIIDD1807 pKa = 3.53 GHH1809 pKa = 5.56 TYY1811 pKa = 10.97 SFGDD1815 pKa = 3.38 ASKK1818 pKa = 9.99 TMTQTGNIYY1827 pKa = 10.47 SYY1829 pKa = 11.5 DD1830 pKa = 3.63 ATTHH1834 pKa = 6.0 EE1835 pKa = 4.77 LTVNTQTGSTLNGGTFTVDD1854 pKa = 2.46 MDD1856 pKa = 3.61 TGVAHH1861 pKa = 6.01 FTGPASLTTALTEE1874 pKa = 4.44 NISFQLIDD1882 pKa = 3.47 NDD1884 pKa = 4.06 GDD1886 pKa = 3.69 VSASKK1891 pKa = 10.93 NLGVTIAAADD1901 pKa = 3.78 SGSNVYY1907 pKa = 8.81 DD1908 pKa = 3.38 TTGGHH1913 pKa = 6.08 TLTGTAFNDD1922 pKa = 4.38 TIDD1925 pKa = 4.41 GNDD1928 pKa = 3.68 GNDD1931 pKa = 4.02 TINGNAGHH1939 pKa = 7.14 DD1940 pKa = 3.8 RR1941 pKa = 11.84 LDD1943 pKa = 3.83 GGTGNDD1949 pKa = 3.83 TLNGGDD1955 pKa = 4.38 GNDD1958 pKa = 3.32 VLYY1961 pKa = 11.12 GGIGDD1966 pKa = 3.94 DD1967 pKa = 4.69 TINGDD1972 pKa = 3.48 SGNDD1976 pKa = 3.29 VLIGGKK1982 pKa = 10.59 GNDD1985 pKa = 3.5 ILNGGSGVDD1994 pKa = 3.14 TFVFSSTASDD2004 pKa = 3.34 NGKK2007 pKa = 8.69 DD2008 pKa = 3.58 TIQDD2012 pKa = 3.17 FTVAPKK2018 pKa = 10.62 VAGLVQGDD2026 pKa = 3.72 ILDD2029 pKa = 3.93 ISDD2032 pKa = 5.06 LLTPSTAATFNSSEE2046 pKa = 3.98 AAAGAFLQFVQDD2058 pKa = 3.83 GANTKK2063 pKa = 10.34 VMFDD2067 pKa = 3.62 ADD2069 pKa = 3.75 GSGTSAAPVQVATLTGVNATDD2090 pKa = 4.42 LLHH2093 pKa = 6.93 HH2094 pKa = 6.77 LLDD2097 pKa = 3.92 NGEE2100 pKa = 4.36 IKK2102 pKa = 9.91 TSHH2105 pKa = 6.21
Molecular weight: 212.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.567
IPC_protein 3.643
Toseland 3.389
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.452
Grimsley 3.287
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.088
Thurlkill 3.452
EMBOSS 3.63
Sillero 3.77
Patrickios 1.863
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A7H9BLL4|A0A7H9BLL4_9NEIS HD-GYP domain-containing protein OS=Chitinibacter sp. 2T18 OX=2739434 GN=HQ393_13515 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.58 RR14 pKa = 11.84 THH16 pKa = 6.03 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSVGGVRR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3281
0
3281
1045249
37
3629
318.6
35.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.024 ± 0.053
0.974 ± 0.013
5.184 ± 0.032
5.61 ± 0.043
3.712 ± 0.03
7.143 ± 0.042
2.241 ± 0.023
5.785 ± 0.038
4.556 ± 0.039
10.972 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.024
3.727 ± 0.036
4.403 ± 0.03
4.865 ± 0.04
5.261 ± 0.047
5.954 ± 0.04
5.162 ± 0.055
6.794 ± 0.041
1.413 ± 0.017
2.66 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here