Amino acid dipepetide frequency for Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.257AlaAla: 4.257 ± 0.07
0.66AlaCys: 0.66 ± 0.018
2.935AlaAsp: 2.935 ± 0.037
3.446AlaGlu: 3.446 ± 0.046
2.315AlaPhe: 2.315 ± 0.037
3.047AlaGly: 3.047 ± 0.04
1.098AlaHis: 1.098 ± 0.019
3.653AlaIle: 3.653 ± 0.049
4.001AlaLys: 4.001 ± 0.053
5.577AlaLeu: 5.577 ± 0.059
1.289AlaMet: 1.289 ± 0.023
2.864AlaAsn: 2.864 ± 0.035
2.218AlaPro: 2.218 ± 0.04
2.073AlaGln: 2.073 ± 0.039
2.355AlaArg: 2.355 ± 0.036
4.881AlaSer: 4.881 ± 0.063
3.555AlaThr: 3.555 ± 0.049
3.545AlaVal: 3.545 ± 0.044
0.518AlaTrp: 0.518 ± 0.016
1.664AlaTyr: 1.664 ± 0.028
0.0AlaXaa: 0.0 ± 0.0
Cys
0.618CysAla: 0.618 ± 0.016
0.272CysCys: 0.272 ± 0.011
0.69CysAsp: 0.69 ± 0.019
0.622CysGlu: 0.622 ± 0.016
0.642CysPhe: 0.642 ± 0.018
0.845CysGly: 0.845 ± 0.023
0.325CysHis: 0.325 ± 0.014
0.949CysIle: 0.949 ± 0.017
0.741CysLys: 0.741 ± 0.017
1.271CysLeu: 1.271 ± 0.025
0.267CysMet: 0.267 ± 0.009
0.616CysAsn: 0.616 ± 0.017
0.489CysPro: 0.489 ± 0.016
0.481CysGln: 0.481 ± 0.014
0.481CysArg: 0.481 ± 0.014
1.038CysSer: 1.038 ± 0.022
0.598CysThr: 0.598 ± 0.016
0.78CysVal: 0.78 ± 0.019
0.161CysTrp: 0.161 ± 0.009
0.476CysTyr: 0.476 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.253AspAla: 3.253 ± 0.038
0.632AspCys: 0.632 ± 0.014
4.803AspAsp: 4.803 ± 0.074
5.25AspGlu: 5.25 ± 0.061
2.659AspPhe: 2.659 ± 0.035
3.008AspGly: 3.008 ± 0.037
1.173AspHis: 1.173 ± 0.022
4.278AspIle: 4.278 ± 0.041
3.645AspLys: 3.645 ± 0.042
5.795AspLeu: 5.795 ± 0.054
1.207AspMet: 1.207 ± 0.024
3.054AspAsn: 3.054 ± 0.038
2.642AspPro: 2.642 ± 0.035
2.05AspGln: 2.05 ± 0.03
2.165AspArg: 2.165 ± 0.029
5.103AspSer: 5.103 ± 0.058
3.226AspThr: 3.226 ± 0.038
3.833AspVal: 3.833 ± 0.041
0.689AspTrp: 0.689 ± 0.018
2.103AspTyr: 2.103 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
3.513GluAla: 3.513 ± 0.054
0.666GluCys: 0.666 ± 0.02
4.432GluAsp: 4.432 ± 0.057
6.086GluGlu: 6.086 ± 0.092
2.776GluPhe: 2.776 ± 0.035
2.635GluGly: 2.635 ± 0.032
1.4GluHis: 1.4 ± 0.024
4.205GluIle: 4.205 ± 0.044
5.0GluLys: 5.0 ± 0.06
6.834GluLeu: 6.834 ± 0.062
1.435GluMet: 1.435 ± 0.026
3.937GluAsn: 3.937 ± 0.043
2.234GluPro: 2.234 ± 0.037
2.961GluGln: 2.961 ± 0.039
3.063GluArg: 3.063 ± 0.039
5.168GluSer: 5.168 ± 0.063
3.998GluThr: 3.998 ± 0.05
3.569GluVal: 3.569 ± 0.044
0.715GluTrp: 0.715 ± 0.018
2.362GluTyr: 2.362 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
2.407PheAla: 2.407 ± 0.035
0.537PheCys: 0.537 ± 0.015
2.869PheAsp: 2.869 ± 0.036
2.82PheGlu: 2.82 ± 0.039
1.994PhePhe: 1.994 ± 0.037
2.621PheGly: 2.621 ± 0.04
1.043PheHis: 1.043 ± 0.021
2.833PheIle: 2.833 ± 0.039
3.059PheLys: 3.059 ± 0.038
3.993PheLeu: 3.993 ± 0.049
0.911PheMet: 0.911 ± 0.018
2.568PheAsn: 2.568 ± 0.035
1.777PhePro: 1.777 ± 0.028
1.987PheGln: 1.987 ± 0.031
1.587PheArg: 1.587 ± 0.025
3.544PheSer: 3.544 ± 0.041
2.48PheThr: 2.48 ± 0.036
2.737PheVal: 2.737 ± 0.037
0.522PheTrp: 0.522 ± 0.017
1.553PheTyr: 1.553 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
2.853GlyAla: 2.853 ± 0.045
0.719GlyCys: 0.719 ± 0.017
2.722GlyAsp: 2.722 ± 0.036
2.791GlyGlu: 2.791 ± 0.042
2.469GlyPhe: 2.469 ± 0.038
3.147GlyGly: 3.147 ± 0.057
1.161GlyHis: 1.161 ± 0.024
3.525GlyIle: 3.525 ± 0.038
3.792GlyLys: 3.792 ± 0.043
4.619GlyLeu: 4.619 ± 0.05
1.015GlyMet: 1.015 ± 0.024
2.821GlyAsn: 2.821 ± 0.042
1.678GlyPro: 1.678 ± 0.03
1.755GlyGln: 1.755 ± 0.032
2.096GlyArg: 2.096 ± 0.031
4.99GlySer: 4.99 ± 0.083
3.138GlyThr: 3.138 ± 0.041
3.19GlyVal: 3.19 ± 0.041
0.611GlyTrp: 0.611 ± 0.018
1.879GlyTyr: 1.879 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
1.068HisAla: 1.068 ± 0.021
0.325HisCys: 0.325 ± 0.011
1.305HisAsp: 1.305 ± 0.022
1.358HisGlu: 1.358 ± 0.024
1.021HisPhe: 1.021 ± 0.02
1.229HisGly: 1.229 ± 0.023
0.682HisHis: 0.682 ± 0.02
1.382HisIle: 1.382 ± 0.023
1.266HisLys: 1.266 ± 0.023
2.218HisLeu: 2.218 ± 0.032
0.454HisMet: 0.454 ± 0.014
1.09HisAsn: 1.09 ± 0.024
1.097HisPro: 1.097 ± 0.02
0.954HisGln: 0.954 ± 0.023
0.984HisArg: 0.984 ± 0.02
1.958HisSer: 1.958 ± 0.033
1.133HisThr: 1.133 ± 0.024
1.255HisVal: 1.255 ± 0.026
0.271HisTrp: 0.271 ± 0.01
0.828HisTyr: 0.828 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
3.599IleAla: 3.599 ± 0.045
0.94IleCys: 0.94 ± 0.022
4.196IleAsp: 4.196 ± 0.04
4.044IleGlu: 4.044 ± 0.039
2.711IlePhe: 2.711 ± 0.039
3.176IleGly: 3.176 ± 0.043
1.379IleHis: 1.379 ± 0.025
3.942IleIle: 3.942 ± 0.046
4.278IleLys: 4.278 ± 0.05
5.949IleLeu: 5.949 ± 0.065
1.289IleMet: 1.289 ± 0.025
3.509IleAsn: 3.509 ± 0.044
3.306IlePro: 3.306 ± 0.04
2.46IleGln: 2.46 ± 0.032
2.696IleArg: 2.696 ± 0.035
5.722IleSer: 5.722 ± 0.063
3.66IleThr: 3.66 ± 0.041
3.869IleVal: 3.869 ± 0.043
0.739IleTrp: 0.739 ± 0.016
2.081IleTyr: 2.081 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
3.609LysAla: 3.609 ± 0.048
0.818LysCys: 0.818 ± 0.02
4.14LysAsp: 4.14 ± 0.04
5.04LysGlu: 5.04 ± 0.058
3.013LysPhe: 3.013 ± 0.034
2.865LysGly: 2.865 ± 0.04
1.572LysHis: 1.572 ± 0.03
4.294LysIle: 4.294 ± 0.047
5.938LysLys: 5.938 ± 0.069
7.313LysLeu: 7.313 ± 0.062
1.447LysMet: 1.447 ± 0.026
3.895LysAsn: 3.895 ± 0.044
3.008LysPro: 3.008 ± 0.047
3.038LysGln: 3.038 ± 0.043
3.812LysArg: 3.812 ± 0.044
5.519LysSer: 5.519 ± 0.057
3.913LysThr: 3.913 ± 0.046
4.148LysVal: 4.148 ± 0.044
0.783LysTrp: 0.783 ± 0.019
2.629LysTyr: 2.629 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
5.518LeuAla: 5.518 ± 0.057
1.29LeuCys: 1.29 ± 0.027
5.657LeuAsp: 5.657 ± 0.051
6.272LeuGlu: 6.272 ± 0.068
4.203LeuPhe: 4.203 ± 0.055
4.504LeuGly: 4.504 ± 0.045
2.156LeuHis: 2.156 ± 0.033
5.761LeuIle: 5.761 ± 0.055
7.535LeuLys: 7.535 ± 0.069
9.767LeuLeu: 9.767 ± 0.087
2.008LeuMet: 2.008 ± 0.026
5.495LeuAsn: 5.495 ± 0.057
4.652LeuPro: 4.652 ± 0.043
4.659LeuGln: 4.659 ± 0.047
4.507LeuArg: 4.507 ± 0.043
8.431LeuSer: 8.431 ± 0.062
5.29LeuThr: 5.29 ± 0.05
5.316LeuVal: 5.316 ± 0.052
0.943LeuTrp: 0.943 ± 0.019
3.059LeuTyr: 3.059 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
1.335MetAla: 1.335 ± 0.023
0.248MetCys: 0.248 ± 0.01
1.319MetAsp: 1.319 ± 0.025
1.289MetGlu: 1.289 ± 0.023
0.929MetPhe: 0.929 ± 0.019
1.103MetGly: 1.103 ± 0.023
0.372MetHis: 0.372 ± 0.012
1.298MetIle: 1.298 ± 0.024
1.572MetLys: 1.572 ± 0.028
1.977MetLeu: 1.977 ± 0.032
0.506MetMet: 0.506 ± 0.015
1.254MetAsn: 1.254 ± 0.028
0.759MetPro: 0.759 ± 0.019
0.726MetGln: 0.726 ± 0.018
0.829MetArg: 0.829 ± 0.018
2.031MetSer: 2.031 ± 0.027
1.194MetThr: 1.194 ± 0.021
1.279MetVal: 1.279 ± 0.022
0.178MetTrp: 0.178 ± 0.009
0.604MetTyr: 0.604 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.105AsnAla: 3.105 ± 0.035
0.651AsnCys: 0.651 ± 0.017
3.695AsnAsp: 3.695 ± 0.037
3.797AsnGlu: 3.797 ± 0.039
2.261AsnPhe: 2.261 ± 0.032
3.424AsnGly: 3.424 ± 0.043
1.166AsnHis: 1.166 ± 0.023
3.576AsnIle: 3.576 ± 0.041
3.702AsnLys: 3.702 ± 0.042
4.821AsnLeu: 4.821 ± 0.046
1.121AsnMet: 1.121 ± 0.024
3.574AsnAsn: 3.574 ± 0.062
2.384AsnPro: 2.384 ± 0.032
2.047AsnGln: 2.047 ± 0.033
2.137AsnArg: 2.137 ± 0.029
4.999AsnSer: 4.999 ± 0.057
3.19AsnThr: 3.19 ± 0.043
3.501AsnVal: 3.501 ± 0.041
0.635AsnTrp: 0.635 ± 0.018
1.861AsnTyr: 1.861 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
2.364ProAla: 2.364 ± 0.036
0.367ProCys: 0.367 ± 0.014
2.295ProAsp: 2.295 ± 0.028
3.113ProGlu: 3.113 ± 0.037
1.878ProPhe: 1.878 ± 0.03
2.022ProGly: 2.022 ± 0.034
0.902ProHis: 0.902 ± 0.02
2.727ProIle: 2.727 ± 0.037
2.815ProLys: 2.815 ± 0.035
4.074ProLeu: 4.074 ± 0.042
0.868ProMet: 0.868 ± 0.02
2.301ProAsn: 2.301 ± 0.032
2.263ProPro: 2.263 ± 0.055
1.893ProGln: 1.893 ± 0.043
1.687ProArg: 1.687 ± 0.024
4.044ProSer: 4.044 ± 0.055
2.759ProThr: 2.759 ± 0.035
2.843ProVal: 2.843 ± 0.036
0.451ProTrp: 0.451 ± 0.013
1.366ProTyr: 1.366 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
2.035GlnAla: 2.035 ± 0.038
0.495GlnCys: 0.495 ± 0.013
2.211GlnAsp: 2.211 ± 0.029
2.788GlnGlu: 2.788 ± 0.045
1.951GlnPhe: 1.951 ± 0.03
1.755GlnGly: 1.755 ± 0.032
1.036GlnHis: 1.036 ± 0.025
2.421GlnIle: 2.421 ± 0.031
2.666GlnLys: 2.666 ± 0.039
4.593GlnLeu: 4.593 ± 0.047
0.898GlnMet: 0.898 ± 0.022
2.204GlnAsn: 2.204 ± 0.031
1.804GlnPro: 1.804 ± 0.041
3.45GlnGln: 3.45 ± 0.136
2.041GlnArg: 2.041 ± 0.029
3.22GlnSer: 3.22 ± 0.044
2.196GlnThr: 2.196 ± 0.033
2.221GlnVal: 2.221 ± 0.029
0.502GlnTrp: 0.502 ± 0.014
1.507GlnTyr: 1.507 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
2.281ArgAla: 2.281 ± 0.031
0.592ArgCys: 0.592 ± 0.012
2.41ArgAsp: 2.41 ± 0.033
2.7ArgGlu: 2.7 ± 0.039
1.987ArgPhe: 1.987 ± 0.032
2.016ArgGly: 2.016 ± 0.036
1.019ArgHis: 1.019 ± 0.021
2.852ArgIle: 2.852 ± 0.032
3.485ArgLys: 3.485 ± 0.045
4.215ArgLeu: 4.215 ± 0.046
0.925ArgMet: 0.925 ± 0.019
2.446ArgAsn: 2.446 ± 0.034
1.632ArgPro: 1.632 ± 0.027
1.784ArgGln: 1.784 ± 0.028
2.703ArgArg: 2.703 ± 0.043
3.574ArgSer: 3.574 ± 0.04
2.37ArgThr: 2.37 ± 0.032
2.442ArgVal: 2.442 ± 0.033
0.494ArgTrp: 0.494 ± 0.016
1.593ArgTyr: 1.593 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
4.727SerAla: 4.727 ± 0.054
0.961SerCys: 0.961 ± 0.019
5.004SerAsp: 5.004 ± 0.057
4.982SerGlu: 4.982 ± 0.052
3.799SerPhe: 3.799 ± 0.044
4.658SerGly: 4.658 ± 0.069
1.908SerHis: 1.908 ± 0.031
5.573SerIle: 5.573 ± 0.05
6.218SerLys: 6.218 ± 0.06
8.42SerLeu: 8.42 ± 0.064
1.848SerMet: 1.848 ± 0.032
5.257SerAsn: 5.257 ± 0.063
3.594SerPro: 3.594 ± 0.056
3.487SerGln: 3.487 ± 0.05
3.605SerArg: 3.605 ± 0.044
9.812SerSer: 9.812 ± 0.16
5.784SerThr: 5.784 ± 0.117
5.226SerVal: 5.226 ± 0.054
0.869SerTrp: 0.869 ± 0.019
2.563SerTyr: 2.563 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
3.587ThrAla: 3.587 ± 0.041
0.651ThrCys: 0.651 ± 0.019
3.283ThrAsp: 3.283 ± 0.038
3.739ThrGlu: 3.739 ± 0.07
2.453ThrPhe: 2.453 ± 0.034
3.411ThrGly: 3.411 ± 0.058
1.159ThrHis: 1.159 ± 0.022
3.7ThrIle: 3.7 ± 0.037
3.922ThrLys: 3.922 ± 0.043
5.449ThrLeu: 5.449 ± 0.049
1.181ThrMet: 1.181 ± 0.02
3.104ThrAsn: 3.104 ± 0.042
2.829ThrPro: 2.829 ± 0.047
2.017ThrGln: 2.017 ± 0.029
2.334ThrArg: 2.334 ± 0.032
5.415ThrSer: 5.415 ± 0.098
3.931ThrThr: 3.931 ± 0.084
3.833ThrVal: 3.833 ± 0.048
0.598ThrTrp: 0.598 ± 0.016
1.708ThrTyr: 1.708 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
3.549ValAla: 3.549 ± 0.04
0.795ValCys: 0.795 ± 0.02
3.721ValAsp: 3.721 ± 0.038
4.019ValGlu: 4.019 ± 0.045
2.626ValPhe: 2.626 ± 0.036
3.029ValGly: 3.029 ± 0.044
1.233ValHis: 1.233 ± 0.024
3.842ValIle: 3.842 ± 0.044
4.124ValLys: 4.124 ± 0.043
5.776ValLeu: 5.776 ± 0.052
1.213ValMet: 1.213 ± 0.018
3.133ValAsn: 3.133 ± 0.035
3.0ValPro: 3.0 ± 0.04
2.241ValGln: 2.241 ± 0.03
2.503ValArg: 2.503 ± 0.033
5.302ValSer: 5.302 ± 0.054
3.541ValThr: 3.541 ± 0.041
3.821ValVal: 3.821 ± 0.043
0.632ValTrp: 0.632 ± 0.018
1.909ValTyr: 1.909 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
0.516TrpAla: 0.516 ± 0.016
0.243TrpCys: 0.243 ± 0.011
0.714TrpAsp: 0.714 ± 0.017
0.666TrpGlu: 0.666 ± 0.016
0.573TrpPhe: 0.573 ± 0.017
0.519TrpGly: 0.519 ± 0.018
0.206TrpHis: 0.206 ± 0.009
0.714TrpIle: 0.714 ± 0.017
0.917TrpLys: 0.917 ± 0.019
1.025TrpLeu: 1.025 ± 0.022
0.225TrpMet: 0.225 ± 0.01
0.714TrpAsn: 0.714 ± 0.018
0.32TrpPro: 0.32 ± 0.013
0.389TrpGln: 0.389 ± 0.012
0.506TrpArg: 0.506 ± 0.015
0.852TrpSer: 0.852 ± 0.019
0.579TrpThr: 0.579 ± 0.018
0.59TrpVal: 0.59 ± 0.017
0.165TrpTrp: 0.165 ± 0.009
0.456TrpTyr: 0.456 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.758TyrAla: 1.758 ± 0.033
0.5TyrCys: 0.5 ± 0.016
2.169TyrAsp: 2.169 ± 0.034
2.168TyrGlu: 2.168 ± 0.029
1.582TyrPhe: 1.582 ± 0.026
1.957TyrGly: 1.957 ± 0.034
0.886TyrHis: 0.886 ± 0.022
2.02TyrIle: 2.02 ± 0.029
2.115TyrLys: 2.115 ± 0.032
3.336TyrLeu: 3.336 ± 0.039
0.696TyrMet: 0.696 ± 0.016
1.811TyrAsn: 1.811 ± 0.029
1.346TyrPro: 1.346 ± 0.023
1.545TyrGln: 1.545 ± 0.028
1.428TyrArg: 1.428 ± 0.027
2.693TyrSer: 2.693 ± 0.038
1.768TyrThr: 1.768 ± 0.03
2.009TyrVal: 2.009 ± 0.03
0.413TyrTrp: 0.413 ± 0.014
1.379TyrTyr: 1.379 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5071 proteins (2461417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski