Woodchuck hepatitis B virus (isolate 7) (WHV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus; Woodchuck hepatitis virus

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P12909-3|HBSAG-3_WHV4 Isoform of P12909 Isoform S of Large envelope protein OS=Woodchuck hepatitis B virus (isolate 7) OX=10432 GN=S PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 10.29NQTFHH7 pKa = 6.73LQGFVDD13 pKa = 4.52GLRR16 pKa = 11.84DD17 pKa = 3.43LTTTEE22 pKa = 4.21RR23 pKa = 11.84QHH25 pKa = 6.18NAYY28 pKa = 9.77GDD30 pKa = 3.87PFTTLSPAVPTVSTILSPPSTTGDD54 pKa = 3.51PALSPEE60 pKa = 4.29MSPSSLLGLLAGLQVVYY77 pKa = 8.81FLWTKK82 pKa = 10.46ILTIAQNLDD91 pKa = 3.17WWWTSLSFPGGIPEE105 pKa = 4.19CTGQNSQFQTCKK117 pKa = 10.26HH118 pKa = 6.26LPTSCPPTCNGFRR131 pKa = 11.84WMYY134 pKa = 9.79LRR136 pKa = 11.84RR137 pKa = 11.84FIIYY141 pKa = 10.36LLVLLLCLIFLLVLLDD157 pKa = 3.72WKK159 pKa = 11.04GLIPVCPLQPTTEE172 pKa = 4.14TTVNCRR178 pKa = 11.84QCTISAQDD186 pKa = 3.8MYY188 pKa = 10.67TPPYY192 pKa = 9.37CCCLKK197 pKa = 9.44PTAGNCTCWPIPSSWALGNYY217 pKa = 7.96LWEE220 pKa = 3.82WALARR225 pKa = 11.84FSWLNLLVPLLQWLGGISLIAWFLLIWMIWFWGPALLSILPPFIPIFVLFFLIWVYY281 pKa = 10.84II282 pKa = 3.78

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P12909-2|HBSAG-2_WHV4 Isoform of P12909 Isoform M of Large envelope protein OS=Woodchuck hepatitis B virus (isolate 7) OX=10432 GN=S PE=3 SV=1
MM1 pKa = 8.03DD2 pKa = 5.37IDD4 pKa = 4.05PYY6 pKa = 11.45KK7 pKa = 10.72EE8 pKa = 4.12FGSSYY13 pKa = 10.8QLLNFLPLDD22 pKa = 3.88FFPDD26 pKa = 3.91LNALVDD32 pKa = 3.85TATALYY38 pKa = 10.2EE39 pKa = 4.05EE40 pKa = 5.01EE41 pKa = 4.13LTGRR45 pKa = 11.84EE46 pKa = 4.29HH47 pKa = 7.77CSPHH51 pKa = 5.3HH52 pKa = 4.74TAIRR56 pKa = 11.84QALVCWDD63 pKa = 4.27EE64 pKa = 4.07LTKK67 pKa = 11.0LIAWMSSNITSEE79 pKa = 4.01QVRR82 pKa = 11.84TIIVNHH88 pKa = 6.0VNDD91 pKa = 3.34TWGLKK96 pKa = 9.17VRR98 pKa = 11.84QSLWFHH104 pKa = 6.39LSCLTFGQHH113 pKa = 5.07TVQEE117 pKa = 4.43FLVSFGVWIRR127 pKa = 11.84TPAPYY132 pKa = 9.94RR133 pKa = 11.84PPNAPILSTLPEE145 pKa = 3.9HH146 pKa = 5.34TVIRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84GGARR156 pKa = 11.84ASRR159 pKa = 11.84SPRR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84TPSPRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84SQSPRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84RR180 pKa = 11.84SQSPSANCC188 pKa = 3.86

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

2

7

2366

141

884

338.0

38.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.41 ± 0.522

3.297 ± 0.583

2.959 ± 0.323

2.578 ± 0.371

4.818 ± 0.212

5.156 ± 0.218

2.62 ± 0.618

5.325 ± 0.68

2.959 ± 0.501

13.187 ± 0.979

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.395 ± 0.186

4.353 ± 0.626

8.411 ± 0.689

4.353 ± 0.279

5.664 ± 1.073

8.538 ± 0.957

7.27 ± 0.54

4.903 ± 0.25

3.888 ± 0.751

2.916 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski