Maliponia aquimaris
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5072 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238KCP0|A0A238KCP0_9RHOB Pyruvate dehydrogenase complex repressor OS=Maliponia aquimaris OX=1673631 GN=pdhR PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.23 KK3 pKa = 9.84 ILFATTALVATAGLAAAEE21 pKa = 4.32 VKK23 pKa = 9.42 VTGSAEE29 pKa = 3.85 MGVIDD34 pKa = 5.91 GDD36 pKa = 4.44 DD37 pKa = 3.33 LATAQFHH44 pKa = 6.73 TDD46 pKa = 2.69 TDD48 pKa = 3.55 ITFTLSGEE56 pKa = 4.16 ADD58 pKa = 3.18 NGLTFGSKK66 pKa = 9.73 IKK68 pKa = 10.81 LEE70 pKa = 4.04 EE71 pKa = 4.04 AAGGIATTVEE81 pKa = 3.77 DD82 pKa = 4.37 DD83 pKa = 3.8 FAVFIAYY90 pKa = 9.64 GGARR94 pKa = 11.84 LTMGDD99 pKa = 3.37 TDD101 pKa = 5.23 GAFDD105 pKa = 3.46 WAMKK109 pKa = 9.91 EE110 pKa = 3.94 VGIGGSIADD119 pKa = 4.36 DD120 pKa = 3.86 HH121 pKa = 5.96 TTHH124 pKa = 7.39 AGFNFNSGLDD134 pKa = 3.78 GTHH137 pKa = 7.43 DD138 pKa = 3.72 GQIARR143 pKa = 11.84 FDD145 pKa = 3.63 YY146 pKa = 10.83 TFDD149 pKa = 3.62 AFSVAISAEE158 pKa = 3.9 IDD160 pKa = 3.34 DD161 pKa = 4.61 TGVVDD166 pKa = 4.32 PVWGIGFKK174 pKa = 9.93 YY175 pKa = 9.43 TGDD178 pKa = 3.7 LAGLTLGVGIGYY190 pKa = 9.97 QSVDD194 pKa = 3.74 FGGGFDD200 pKa = 3.61 VQVAGISLDD209 pKa = 3.61 TTFDD213 pKa = 3.52 NGLMAILNYY222 pKa = 10.46 SEE224 pKa = 4.05 TDD226 pKa = 3.16 IAGTTVDD233 pKa = 3.19 HH234 pKa = 6.89 WGLGLGYY241 pKa = 8.33 TVNALTVSANYY252 pKa = 10.49 GEE254 pKa = 4.38 YY255 pKa = 10.56 SFPGSLKK262 pKa = 10.87 DD263 pKa = 3.4 SGFGLAVNYY272 pKa = 10.29 DD273 pKa = 3.58 LGGGLVAQFGYY284 pKa = 10.71 GSSSVDD290 pKa = 2.87 VGPAVFGIGDD300 pKa = 3.6 GRR302 pKa = 11.84 FAGALPGDD310 pKa = 3.75 YY311 pKa = 10.44 DD312 pKa = 3.97 SYY314 pKa = 11.82 SFGLAMSFF322 pKa = 3.96
Molecular weight: 33.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.516
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.884
Patrickios 1.888
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A238KBE9|A0A238KBE9_9RHOB Internalin-A OS=Maliponia aquimaris OX=1673631 GN=inlA_1 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.5 GGRR28 pKa = 11.84 KK29 pKa = 8.99 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5072
0
5072
1572961
29
3640
310.1
33.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.852 ± 0.05
0.936 ± 0.012
6.097 ± 0.032
5.627 ± 0.038
3.596 ± 0.022
8.932 ± 0.044
2.067 ± 0.018
4.708 ± 0.027
2.742 ± 0.029
10.289 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.017
2.318 ± 0.02
5.408 ± 0.029
3.101 ± 0.017
7.224 ± 0.041
4.796 ± 0.027
5.55 ± 0.035
7.434 ± 0.032
1.469 ± 0.015
2.174 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here