Wolbachia endosymbiont wVitA of Nasonia vitripennis phage WOVitA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9P5Y1|E9P5Y1_9CAUD Putative virulence protein VrlC.2 OS=Wolbachia endosymbiont wVitA of Nasonia vitripennis phage WOVitA1 OX=949124 PE=4 SV=1
MM1 pKa = 7.4TLNSYY6 pKa = 9.26YY7 pKa = 11.01NRR9 pKa = 11.84FNPDD13 pKa = 2.6KK14 pKa = 9.93EE15 pKa = 4.45YY16 pKa = 11.14EE17 pKa = 4.1KK18 pKa = 11.26SLFLAGRR25 pKa = 11.84GLQSAEE31 pKa = 3.83LNEE34 pKa = 4.2TQEE37 pKa = 4.28YY38 pKa = 10.71ALSKK42 pKa = 10.85LKK44 pKa = 11.13GIGDD48 pKa = 4.62AIFRR52 pKa = 11.84DD53 pKa = 3.75GDD55 pKa = 3.88VITGSNCIIDD65 pKa = 4.07RR66 pKa = 11.84EE67 pKa = 4.45TGKK70 pKa = 8.52VTLEE74 pKa = 3.66SGKK77 pKa = 10.1IYY79 pKa = 10.61LRR81 pKa = 11.84GAVRR85 pKa = 11.84KK86 pKa = 9.17VEE88 pKa = 4.08KK89 pKa = 10.55EE90 pKa = 3.86EE91 pKa = 3.91FVIPLSTTVRR101 pKa = 11.84IGVYY105 pKa = 9.22YY106 pKa = 10.39VEE108 pKa = 4.3STITEE113 pKa = 4.26LEE115 pKa = 4.21DD116 pKa = 3.35EE117 pKa = 4.55NLRR120 pKa = 11.84DD121 pKa = 3.55PAIGTRR127 pKa = 11.84NYY129 pKa = 10.54QEE131 pKa = 3.78VGAARR136 pKa = 11.84LKK138 pKa = 10.67ISTIWGYY145 pKa = 7.57QAEE148 pKa = 4.4GLSPRR153 pKa = 11.84FSEE156 pKa = 4.7GEE158 pKa = 3.83FYY160 pKa = 10.49PIYY163 pKa = 10.54NIEE166 pKa = 4.08NGVLIEE172 pKa = 4.85HH173 pKa = 6.79SPPPQANIVTTALARR188 pKa = 11.84YY189 pKa = 9.06DD190 pKa = 3.57KK191 pKa = 9.9EE192 pKa = 4.82ANGSYY197 pKa = 9.87VVNGLEE203 pKa = 4.41VMFLQKK209 pKa = 11.01EE210 pKa = 4.04EE211 pKa = 4.63GEE213 pKa = 4.33GGKK216 pKa = 10.4KK217 pKa = 9.7IFVINEE223 pKa = 3.86GKK225 pKa = 10.3AHH227 pKa = 5.83VDD229 pKa = 3.42GYY231 pKa = 10.76EE232 pKa = 3.51IEE234 pKa = 4.62LPHH237 pKa = 7.14SIRR240 pKa = 11.84ISFDD244 pKa = 3.15EE245 pKa = 4.85DD246 pKa = 3.65PDD248 pKa = 3.74IKK250 pKa = 10.84SVEE253 pKa = 4.35SEE255 pKa = 3.84PHH257 pKa = 5.03TFQPNSQRR265 pKa = 11.84VMEE268 pKa = 4.64LKK270 pKa = 11.15VNDD273 pKa = 4.17FPISEE278 pKa = 4.18IKK280 pKa = 10.58KK281 pKa = 10.0VDD283 pKa = 2.99ITIQKK288 pKa = 8.83TITITHH294 pKa = 6.78GSYY297 pKa = 10.42SGAIDD302 pKa = 5.63PIPDD306 pKa = 3.53SAVLEE311 pKa = 4.54IIQAKK316 pKa = 8.26QGNVIYY322 pKa = 10.13EE323 pKa = 3.81NSVDD327 pKa = 3.77YY328 pKa = 10.77KK329 pKa = 11.34LNAGNVDD336 pKa = 2.72WSLPGKK342 pKa = 9.9EE343 pKa = 4.11PAPGSSYY350 pKa = 10.72QITYY354 pKa = 9.83RR355 pKa = 11.84CRR357 pKa = 11.84THH359 pKa = 6.91VSPEE363 pKa = 4.38DD364 pKa = 3.55ISEE367 pKa = 4.21EE368 pKa = 4.29GCKK371 pKa = 10.67VKK373 pKa = 10.77GAVDD377 pKa = 3.53NSLVLIDD384 pKa = 4.7YY385 pKa = 6.81TWKK388 pKa = 9.73MPRR391 pKa = 11.84FDD393 pKa = 6.42LITIDD398 pKa = 3.12SKK400 pKa = 11.48GVVRR404 pKa = 11.84RR405 pKa = 11.84IKK407 pKa = 10.75GIAHH411 pKa = 7.37PWRR414 pKa = 11.84PSMPRR419 pKa = 11.84APSGQLLLCYY429 pKa = 10.21IHH431 pKa = 5.87QTWKK435 pKa = 10.49NGEE438 pKa = 3.99GVKK441 pKa = 9.94IVNNAIHH448 pKa = 6.62AVPMNEE454 pKa = 3.93LEE456 pKa = 4.22AMKK459 pKa = 10.52KK460 pKa = 10.43GINDD464 pKa = 4.22LYY466 pKa = 11.47ALVAEE471 pKa = 4.41EE472 pKa = 4.7RR473 pKa = 11.84LRR475 pKa = 11.84SDD477 pKa = 3.24ANSRR481 pKa = 11.84EE482 pKa = 4.13PTTKK486 pKa = 10.14KK487 pKa = 10.7GVFVDD492 pKa = 4.65SFFDD496 pKa = 4.97DD497 pKa = 3.92DD498 pKa = 4.83MRR500 pKa = 11.84DD501 pKa = 3.34QGISQSAAIVNKK513 pKa = 10.03EE514 pKa = 4.43LILPIDD520 pKa = 3.68VTIADD525 pKa = 3.53IDD527 pKa = 4.11GGEE530 pKa = 4.06KK531 pKa = 9.92TYY533 pKa = 11.02LLPYY537 pKa = 9.52EE538 pKa = 4.66LEE540 pKa = 4.31PVLEE544 pKa = 4.23QLLQTKK550 pKa = 9.14GEE552 pKa = 4.45KK553 pKa = 9.8INPYY557 pKa = 9.47QAFDD561 pKa = 3.98PVPAQITLNKK571 pKa = 10.41NIDD574 pKa = 3.08HH575 pKa = 5.88WTEE578 pKa = 3.68VTTNWKK584 pKa = 10.57SPVTRR589 pKa = 11.84VFNVKK594 pKa = 8.07EE595 pKa = 4.29TTEE598 pKa = 4.3LLSSTSYY605 pKa = 9.44EE606 pKa = 3.91AEE608 pKa = 4.02FMRR611 pKa = 11.84EE612 pKa = 3.74AVQDD616 pKa = 3.71FEE618 pKa = 5.51IEE620 pKa = 4.04GFEE623 pKa = 4.03PNEE626 pKa = 3.67RR627 pKa = 11.84LKK629 pKa = 10.66EE630 pKa = 3.96IKK632 pKa = 9.95FDD634 pKa = 3.63GMSIQPTAA642 pKa = 3.52

Molecular weight: 72.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9P5X2|E9P5X2_9CAUD Uncharacterized protein OS=Wolbachia endosymbiont wVitA of Nasonia vitripennis phage WOVitA1 OX=949124 PE=4 SV=1
MM1 pKa = 7.54LRR3 pKa = 11.84PFGKK7 pKa = 10.08IKK9 pKa = 10.37IFAKK13 pKa = 10.36NSIVFKK19 pKa = 10.97LLMFSCFIYY28 pKa = 9.82TLRR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.57GFLKK37 pKa = 10.64KK38 pKa = 10.65GG39 pKa = 3.39

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

20107

39

2474

319.2

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.709 ± 0.332

1.025 ± 0.125

5.003 ± 0.156

8.087 ± 0.373

3.481 ± 0.175

6.102 ± 0.22

2.079 ± 0.203

8.092 ± 0.271

8.863 ± 0.288

9.723 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.726 ± 0.143

6.058 ± 0.446

2.989 ± 0.212

3.098 ± 0.134

4.6 ± 0.3

6.729 ± 0.221

4.958 ± 0.192

6.495 ± 0.181

0.935 ± 0.142

3.248 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski