Gordonia phage Verity

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DIP9|A0A514DIP9_9CAUD Uncharacterized protein OS=Gordonia phage Verity OX=2591211 GN=5 PE=4 SV=1
MM1 pKa = 7.36YY2 pKa = 9.61EE3 pKa = 4.21LLAALTLIATGPSQAPAPTPVACVMEE29 pKa = 4.29TTPGDD34 pKa = 3.92FAPCPPPIVSTSGGDD49 pKa = 4.16SIGGDD54 pKa = 3.39ANSDD58 pKa = 3.75PVNMRR63 pKa = 11.84DD64 pKa = 3.48LPRR67 pKa = 11.84LGGEE71 pKa = 4.28YY72 pKa = 9.75EE73 pKa = 4.49YY74 pKa = 11.16KK75 pKa = 10.71SPTPEE80 pKa = 4.58PEE82 pKa = 4.41AEE84 pKa = 4.22TPDD87 pKa = 3.67EE88 pKa = 4.63GEE90 pKa = 4.03EE91 pKa = 4.24TPADD95 pKa = 3.92GEE97 pKa = 4.3

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DIW8|A0A514DIW8_9CAUD Uncharacterized protein OS=Gordonia phage Verity OX=2591211 GN=72 PE=4 SV=1
MM1 pKa = 7.55TDD3 pKa = 3.63DD4 pKa = 3.64RR5 pKa = 11.84PHH7 pKa = 6.49RR8 pKa = 11.84GWYY11 pKa = 10.38RR12 pKa = 11.84LMNPLAARR20 pKa = 11.84RR21 pKa = 11.84PPARR25 pKa = 11.84GSQPEE30 pKa = 4.5VVLSTPPLPTLLINDD45 pKa = 4.13DD46 pKa = 3.34WRR48 pKa = 11.84DD49 pKa = 3.87TIRR52 pKa = 11.84GSATIAIGTPDD63 pKa = 4.85GIHH66 pKa = 5.99TRR68 pKa = 11.84VSLDD72 pKa = 3.27RR73 pKa = 11.84NAVTDD78 pKa = 4.58DD79 pKa = 4.04DD80 pKa = 4.23PDD82 pKa = 3.93YY83 pKa = 11.45AVVLAHH89 pKa = 5.42PTRR92 pKa = 11.84RR93 pKa = 11.84VGATITKK100 pKa = 7.68ATPGKK105 pKa = 10.05KK106 pKa = 9.53RR107 pKa = 11.84SRR109 pKa = 3.64

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

19583

32

1636

222.5

24.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.036 ± 0.529

0.929 ± 0.174

7.491 ± 0.472

5.295 ± 0.342

2.216 ± 0.206

8.64 ± 0.532

2.094 ± 0.208

4.647 ± 0.217

2.727 ± 0.155

7.537 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.102

2.732 ± 0.19

6.393 ± 0.257

3.723 ± 0.168

7.455 ± 0.464

4.734 ± 0.239

7.221 ± 0.276

7.619 ± 0.204

2.043 ± 0.147

2.283 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski