Amino acid dipepetide frequency for Gordonia phage Verity

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.954AlaAla: 16.954 ± 1.701
1.072AlaCys: 1.072 ± 0.291
9.243AlaAsp: 9.243 ± 0.816
7.66AlaGlu: 7.66 ± 0.709
2.758AlaPhe: 2.758 ± 0.518
10.213AlaGly: 10.213 ± 1.302
1.941AlaHis: 1.941 ± 0.289
5.668AlaIle: 5.668 ± 0.729
3.932AlaLys: 3.932 ± 0.423
8.784AlaLeu: 8.784 ± 0.865
2.043AlaMet: 2.043 ± 0.261
3.319AlaAsn: 3.319 ± 0.395
5.924AlaPro: 5.924 ± 0.603
5.311AlaGln: 5.311 ± 0.567
8.528AlaArg: 8.528 ± 0.691
5.209AlaSer: 5.209 ± 0.464
7.711AlaThr: 7.711 ± 0.716
9.192AlaVal: 9.192 ± 0.581
2.145AlaTrp: 2.145 ± 0.354
2.758AlaTyr: 2.758 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
1.175CysAla: 1.175 ± 0.36
0.051CysCys: 0.051 ± 0.053
0.511CysAsp: 0.511 ± 0.175
0.255CysGlu: 0.255 ± 0.102
0.204CysPhe: 0.204 ± 0.088
1.379CysGly: 1.379 ± 0.363
0.306CysHis: 0.306 ± 0.126
0.46CysIle: 0.46 ± 0.185
0.204CysLys: 0.204 ± 0.097
0.46CysLeu: 0.46 ± 0.128
0.0CysMet: 0.0 ± 0.0
0.153CysAsn: 0.153 ± 0.086
0.919CysPro: 0.919 ± 0.235
0.051CysGln: 0.051 ± 0.045
1.123CysArg: 1.123 ± 0.257
0.613CysSer: 0.613 ± 0.217
0.562CysThr: 0.562 ± 0.196
0.357CysVal: 0.357 ± 0.156
0.204CysTrp: 0.204 ± 0.115
0.306CysTyr: 0.306 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
7.966AspAla: 7.966 ± 0.69
0.664AspCys: 0.664 ± 0.164
7.609AspAsp: 7.609 ± 1.078
6.383AspGlu: 6.383 ± 0.83
0.919AspPhe: 0.919 ± 0.251
7.354AspGly: 7.354 ± 0.605
1.328AspHis: 1.328 ± 0.303
2.553AspIle: 2.553 ± 0.315
1.787AspLys: 1.787 ± 0.359
5.056AspLeu: 5.056 ± 0.497
1.685AspMet: 1.685 ± 0.313
2.094AspAsn: 2.094 ± 0.313
6.281AspPro: 6.281 ± 0.553
2.094AspGln: 2.094 ± 0.458
5.362AspArg: 5.362 ± 0.753
2.758AspSer: 2.758 ± 0.356
5.26AspThr: 5.26 ± 0.519
4.851AspVal: 4.851 ± 0.628
1.583AspTrp: 1.583 ± 0.286
1.328AspTyr: 1.328 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
5.362GluAla: 5.362 ± 0.617
0.409GluCys: 0.409 ± 0.154
1.941GluAsp: 1.941 ± 0.307
1.328GluGlu: 1.328 ± 0.293
2.196GluPhe: 2.196 ± 0.329
3.575GluGly: 3.575 ± 0.383
1.736GluHis: 1.736 ± 0.295
2.86GluIle: 2.86 ± 0.369
1.226GluLys: 1.226 ± 0.275
5.464GluLeu: 5.464 ± 0.636
1.175GluMet: 1.175 ± 0.274
1.123GluAsn: 1.123 ± 0.259
2.604GluPro: 2.604 ± 0.38
3.473GluGln: 3.473 ± 0.559
4.085GluArg: 4.085 ± 0.649
2.758GluSer: 2.758 ± 0.394
3.473GluThr: 3.473 ± 0.477
4.443GluVal: 4.443 ± 0.413
1.736GluTrp: 1.736 ± 0.306
1.992GluTyr: 1.992 ± 0.356
0.0GluXaa: 0.0 ± 0.0
Phe
2.962PheAla: 2.962 ± 0.436
0.255PheCys: 0.255 ± 0.136
2.451PheAsp: 2.451 ± 0.346
1.175PheGlu: 1.175 ± 0.253
0.766PhePhe: 0.766 ± 0.318
2.502PheGly: 2.502 ± 0.388
0.357PheHis: 0.357 ± 0.13
0.919PheIle: 0.919 ± 0.242
0.766PheLys: 0.766 ± 0.201
1.226PheLeu: 1.226 ± 0.22
0.664PheMet: 0.664 ± 0.152
0.715PheAsn: 0.715 ± 0.261
1.021PhePro: 1.021 ± 0.263
0.562PheGln: 0.562 ± 0.158
1.43PheArg: 1.43 ± 0.269
0.919PheSer: 0.919 ± 0.185
1.379PheThr: 1.379 ± 0.28
1.328PheVal: 1.328 ± 0.218
0.409PheTrp: 0.409 ± 0.127
0.357PheTyr: 0.357 ± 0.128
0.0PheXaa: 0.0 ± 0.0
Gly
9.192GlyAla: 9.192 ± 1.065
1.123GlyCys: 1.123 ± 0.326
6.383GlyAsp: 6.383 ± 0.889
3.881GlyGlu: 3.881 ± 0.535
1.583GlyPhe: 1.583 ± 0.419
8.222GlyGly: 8.222 ± 1.064
1.992GlyHis: 1.992 ± 0.328
4.954GlyIle: 4.954 ± 0.645
2.604GlyLys: 2.604 ± 0.364
5.873GlyLeu: 5.873 ± 0.654
2.349GlyMet: 2.349 ± 0.353
2.655GlyAsn: 2.655 ± 0.521
4.034GlyPro: 4.034 ± 0.433
3.473GlyGln: 3.473 ± 0.386
6.434GlyArg: 6.434 ± 0.576
4.596GlySer: 4.596 ± 0.648
6.588GlyThr: 6.588 ± 0.798
6.792GlyVal: 6.792 ± 0.731
1.481GlyTrp: 1.481 ± 0.227
2.196GlyTyr: 2.196 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
2.4HisAla: 2.4 ± 0.368
0.255HisCys: 0.255 ± 0.114
2.043HisAsp: 2.043 ± 0.362
1.072HisGlu: 1.072 ± 0.258
0.357HisPhe: 0.357 ± 0.132
1.787HisGly: 1.787 ± 0.296
0.357HisHis: 0.357 ± 0.121
0.817HisIle: 0.817 ± 0.202
0.357HisLys: 0.357 ± 0.14
1.889HisLeu: 1.889 ± 0.365
0.562HisMet: 0.562 ± 0.177
0.357HisAsn: 0.357 ± 0.109
1.328HisPro: 1.328 ± 0.323
0.817HisGln: 0.817 ± 0.203
2.145HisArg: 2.145 ± 0.368
0.511HisSer: 0.511 ± 0.157
1.532HisThr: 1.532 ± 0.33
1.583HisVal: 1.583 ± 0.279
0.255HisTrp: 0.255 ± 0.122
0.511HisTyr: 0.511 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
6.23IleAla: 6.23 ± 0.559
0.204IleCys: 0.204 ± 0.092
4.085IleAsp: 4.085 ± 0.386
3.166IleGlu: 3.166 ± 0.431
0.46IlePhe: 0.46 ± 0.142
4.902IleGly: 4.902 ± 0.732
0.868IleHis: 0.868 ± 0.163
1.941IleIle: 1.941 ± 0.32
1.226IleLys: 1.226 ± 0.334
2.553IleLeu: 2.553 ± 0.313
1.175IleMet: 1.175 ± 0.191
1.736IleAsn: 1.736 ± 0.322
2.86IlePro: 2.86 ± 0.285
1.328IleGln: 1.328 ± 0.313
2.962IleArg: 2.962 ± 0.445
1.634IleSer: 1.634 ± 0.312
3.728IleThr: 3.728 ± 0.341
3.779IleVal: 3.779 ± 0.362
0.613IleTrp: 0.613 ± 0.178
1.021IleTyr: 1.021 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
2.911LysAla: 2.911 ± 0.307
0.255LysCys: 0.255 ± 0.108
1.583LysAsp: 1.583 ± 0.361
0.715LysGlu: 0.715 ± 0.199
0.97LysPhe: 0.97 ± 0.229
1.992LysGly: 1.992 ± 0.363
0.511LysHis: 0.511 ± 0.142
1.072LysIle: 1.072 ± 0.223
0.511LysLys: 0.511 ± 0.167
3.166LysLeu: 3.166 ± 0.38
0.766LysMet: 0.766 ± 0.174
0.97LysAsn: 0.97 ± 0.28
1.685LysPro: 1.685 ± 0.259
0.868LysGln: 0.868 ± 0.285
2.604LysArg: 2.604 ± 0.311
1.532LysSer: 1.532 ± 0.283
1.532LysThr: 1.532 ± 0.232
1.992LysVal: 1.992 ± 0.365
0.715LysTrp: 0.715 ± 0.179
0.919LysTyr: 0.919 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
9.447LeuAla: 9.447 ± 0.566
0.357LeuCys: 0.357 ± 0.134
5.822LeuAsp: 5.822 ± 0.719
4.188LeuGlu: 4.188 ± 0.535
1.481LeuPhe: 1.481 ± 0.329
7.558LeuGly: 7.558 ± 0.954
1.277LeuHis: 1.277 ± 0.281
4.136LeuIle: 4.136 ± 0.412
1.532LeuLys: 1.532 ± 0.329
5.464LeuLeu: 5.464 ± 0.56
1.226LeuMet: 1.226 ± 0.2
2.094LeuAsn: 2.094 ± 0.312
4.954LeuPro: 4.954 ± 0.51
2.604LeuGln: 2.604 ± 0.378
5.26LeuArg: 5.26 ± 0.652
3.115LeuSer: 3.115 ± 0.364
5.362LeuThr: 5.362 ± 0.508
6.486LeuVal: 6.486 ± 0.54
1.532LeuTrp: 1.532 ± 0.365
1.481LeuTyr: 1.481 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
2.604MetAla: 2.604 ± 0.344
0.051MetCys: 0.051 ± 0.056
0.613MetAsp: 0.613 ± 0.17
0.766MetGlu: 0.766 ± 0.159
0.255MetPhe: 0.255 ± 0.103
1.072MetGly: 1.072 ± 0.233
0.255MetHis: 0.255 ± 0.109
1.43MetIle: 1.43 ± 0.274
0.46MetLys: 0.46 ± 0.174
1.992MetLeu: 1.992 ± 0.332
0.255MetMet: 0.255 ± 0.102
0.766MetAsn: 0.766 ± 0.152
1.992MetPro: 1.992 ± 0.372
0.511MetGln: 0.511 ± 0.164
1.889MetArg: 1.889 ± 0.312
1.379MetSer: 1.379 ± 0.238
3.575MetThr: 3.575 ± 0.367
1.328MetVal: 1.328 ± 0.293
0.409MetTrp: 0.409 ± 0.132
0.255MetTyr: 0.255 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
3.37AsnAla: 3.37 ± 0.544
0.306AsnCys: 0.306 ± 0.104
1.889AsnAsp: 1.889 ± 0.294
1.736AsnGlu: 1.736 ± 0.235
0.255AsnPhe: 0.255 ± 0.125
3.217AsnGly: 3.217 ± 0.363
0.409AsnHis: 0.409 ± 0.127
1.021AsnIle: 1.021 ± 0.291
0.919AsnLys: 0.919 ± 0.221
1.941AsnLeu: 1.941 ± 0.294
0.46AsnMet: 0.46 ± 0.23
0.715AsnAsn: 0.715 ± 0.181
2.758AsnPro: 2.758 ± 0.366
0.613AsnGln: 0.613 ± 0.168
1.328AsnArg: 1.328 ± 0.263
1.379AsnSer: 1.379 ± 0.296
1.685AsnThr: 1.685 ± 0.299
2.196AsnVal: 2.196 ± 0.353
0.715AsnTrp: 0.715 ± 0.206
0.409AsnTyr: 0.409 ± 0.126
0.0AsnXaa: 0.0 ± 0.0
Pro
8.784ProAla: 8.784 ± 0.584
0.255ProCys: 0.255 ± 0.116
5.72ProAsp: 5.72 ± 0.678
3.422ProGlu: 3.422 ± 0.457
1.583ProPhe: 1.583 ± 0.271
4.954ProGly: 4.954 ± 0.56
1.379ProHis: 1.379 ± 0.278
2.502ProIle: 2.502 ± 0.294
1.992ProLys: 1.992 ± 0.304
3.064ProLeu: 3.064 ± 0.484
0.817ProMet: 0.817 ± 0.191
1.583ProAsn: 1.583 ± 0.299
5.72ProPro: 5.72 ± 1.093
1.634ProGln: 1.634 ± 0.296
4.188ProArg: 4.188 ± 0.531
2.809ProSer: 2.809 ± 0.312
5.413ProThr: 5.413 ± 0.609
5.158ProVal: 5.158 ± 0.516
1.379ProTrp: 1.379 ± 0.282
1.583ProTyr: 1.583 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
4.494GlnAla: 4.494 ± 0.499
0.357GlnCys: 0.357 ± 0.132
1.532GlnAsp: 1.532 ± 0.299
1.226GlnGlu: 1.226 ± 0.212
1.226GlnPhe: 1.226 ± 0.218
1.941GlnGly: 1.941 ± 0.243
0.919GlnHis: 0.919 ± 0.185
1.787GlnIle: 1.787 ± 0.277
0.919GlnLys: 0.919 ± 0.189
4.034GlnLeu: 4.034 ± 0.506
1.328GlnMet: 1.328 ± 0.22
0.817GlnAsn: 0.817 ± 0.185
2.145GlnPro: 2.145 ± 0.331
2.758GlnGln: 2.758 ± 0.413
2.809GlnArg: 2.809 ± 0.487
1.838GlnSer: 1.838 ± 0.499
2.298GlnThr: 2.298 ± 0.375
3.217GlnVal: 3.217 ± 0.345
0.817GlnTrp: 0.817 ± 0.176
0.766GlnTyr: 0.766 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
8.426ArgAla: 8.426 ± 0.829
0.919ArgCys: 0.919 ± 0.234
4.494ArgAsp: 4.494 ± 0.457
4.494ArgGlu: 4.494 ± 0.749
1.226ArgPhe: 1.226 ± 0.207
4.698ArgGly: 4.698 ± 0.515
2.247ArgHis: 2.247 ± 0.462
3.677ArgIle: 3.677 ± 0.463
2.962ArgLys: 2.962 ± 0.407
5.362ArgLeu: 5.362 ± 0.519
2.094ArgMet: 2.094 ± 0.299
2.043ArgAsn: 2.043 ± 0.304
3.779ArgPro: 3.779 ± 0.575
2.707ArgGln: 2.707 ± 0.488
8.937ArgArg: 8.937 ± 1.079
3.115ArgSer: 3.115 ± 0.492
4.239ArgThr: 4.239 ± 0.51
5.515ArgVal: 5.515 ± 0.626
1.838ArgTrp: 1.838 ± 0.355
1.787ArgTyr: 1.787 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
5.311SerAla: 5.311 ± 0.776
0.613SerCys: 0.613 ± 0.222
3.319SerAsp: 3.319 ± 0.464
2.043SerGlu: 2.043 ± 0.292
1.328SerPhe: 1.328 ± 0.23
5.515SerGly: 5.515 ± 0.528
1.021SerHis: 1.021 ± 0.203
2.707SerIle: 2.707 ± 0.341
1.072SerLys: 1.072 ± 0.207
3.319SerLeu: 3.319 ± 0.355
1.583SerMet: 1.583 ± 0.282
1.328SerAsn: 1.328 ± 0.215
2.4SerPro: 2.4 ± 0.393
1.532SerGln: 1.532 ± 0.352
2.553SerArg: 2.553 ± 0.416
3.166SerSer: 3.166 ± 0.411
3.575SerThr: 3.575 ± 0.398
2.86SerVal: 2.86 ± 0.369
1.123SerTrp: 1.123 ± 0.239
0.97SerTyr: 0.97 ± 0.276
0.0SerXaa: 0.0 ± 0.0
Thr
9.601ThrAla: 9.601 ± 1.06
0.766ThrCys: 0.766 ± 0.219
5.924ThrAsp: 5.924 ± 0.657
2.962ThrGlu: 2.962 ± 0.337
1.379ThrPhe: 1.379 ± 0.217
5.873ThrGly: 5.873 ± 0.647
1.379ThrHis: 1.379 ± 0.331
3.575ThrIle: 3.575 ± 0.448
1.736ThrLys: 1.736 ± 0.241
5.873ThrLeu: 5.873 ± 0.608
1.838ThrMet: 1.838 ± 0.29
1.634ThrAsn: 1.634 ± 0.331
6.537ThrPro: 6.537 ± 0.73
1.889ThrGln: 1.889 ± 0.306
4.239ThrArg: 4.239 ± 0.561
3.524ThrSer: 3.524 ± 0.456
5.566ThrThr: 5.566 ± 0.633
5.822ThrVal: 5.822 ± 0.597
0.97ThrTrp: 0.97 ± 0.218
1.072ThrTyr: 1.072 ± 0.216
0.0ThrXaa: 0.0 ± 0.0
Val
8.12ValAla: 8.12 ± 0.655
0.817ValCys: 0.817 ± 0.224
6.588ValAsp: 6.588 ± 0.742
4.8ValGlu: 4.8 ± 0.547
1.736ValPhe: 1.736 ± 0.343
6.639ValGly: 6.639 ± 0.593
1.583ValHis: 1.583 ± 0.285
2.86ValIle: 2.86 ± 0.384
1.532ValLys: 1.532 ± 0.29
5.617ValLeu: 5.617 ± 0.498
0.868ValMet: 0.868 ± 0.176
2.247ValAsn: 2.247 ± 0.337
4.494ValPro: 4.494 ± 0.412
2.911ValGln: 2.911 ± 0.335
4.902ValArg: 4.902 ± 0.674
3.728ValSer: 3.728 ± 0.448
6.281ValThr: 6.281 ± 0.585
6.486ValVal: 6.486 ± 0.685
1.685ValTrp: 1.685 ± 0.337
2.298ValTyr: 2.298 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
2.553TrpAla: 2.553 ± 0.355
0.409TrpCys: 0.409 ± 0.146
0.97TrpAsp: 0.97 ± 0.2
0.715TrpGlu: 0.715 ± 0.187
0.919TrpPhe: 0.919 ± 0.2
1.021TrpGly: 1.021 ± 0.197
0.562TrpHis: 0.562 ± 0.173
0.715TrpIle: 0.715 ± 0.194
0.97TrpLys: 0.97 ± 0.239
2.298TrpLeu: 2.298 ± 0.292
0.357TrpMet: 0.357 ± 0.133
0.409TrpAsn: 0.409 ± 0.194
1.328TrpPro: 1.328 ± 0.246
1.175TrpGln: 1.175 ± 0.205
1.277TrpArg: 1.277 ± 0.304
1.634TrpSer: 1.634 ± 0.364
1.072TrpThr: 1.072 ± 0.268
1.277TrpVal: 1.277 ± 0.261
0.409TrpTrp: 0.409 ± 0.143
0.357TrpTyr: 0.357 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.37
0.153TyrCys: 0.153 ± 0.097
2.196TyrAsp: 2.196 ± 0.371
0.97TyrGlu: 0.97 ± 0.272
0.562TyrPhe: 0.562 ± 0.202
1.992TyrGly: 1.992 ± 0.338
0.511TyrHis: 0.511 ± 0.191
0.817TyrIle: 0.817 ± 0.214
0.562TyrLys: 0.562 ± 0.169
1.941TyrLeu: 1.941 ± 0.285
0.562TyrMet: 0.562 ± 0.189
0.562TyrAsn: 0.562 ± 0.17
1.175TyrPro: 1.175 ± 0.261
0.919TyrGln: 0.919 ± 0.264
2.502TyrArg: 2.502 ± 0.385
1.123TyrSer: 1.123 ± 0.294
1.379TyrThr: 1.379 ± 0.307
1.532TyrVal: 1.532 ± 0.299
0.409TyrTrp: 0.409 ± 0.126
0.46TyrTyr: 0.46 ± 0.134
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (19583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski