Lachnoanaerobaculum sp. ICM7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoanaerobaculum; unclassified Lachnoanaerobaculum

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J4UCB5|J4UCB5_9FIRM Histidine kinase OS=Lachnoanaerobaculum sp. ICM7 OX=936594 GN=HMPREF1140_0868 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 4.88KK3 pKa = 10.91FIMYY7 pKa = 10.34AKK9 pKa = 9.84EE10 pKa = 3.66QFGYY14 pKa = 10.63DD15 pKa = 3.16ISLTEE20 pKa = 5.04ADD22 pKa = 4.0TPDD25 pKa = 3.38TFEE28 pKa = 4.52SLFGVSFIAEE38 pKa = 4.25EE39 pKa = 4.11EE40 pKa = 4.23NTAVSYY46 pKa = 10.56DD47 pKa = 3.34EE48 pKa = 4.32KK49 pKa = 10.94FIYY52 pKa = 10.61KK53 pKa = 9.74NDD55 pKa = 3.56SAIVANMDD63 pKa = 3.47TVYY66 pKa = 11.11LDD68 pKa = 4.7DD69 pKa = 5.28GCIQQDD75 pKa = 2.91IGLAAA80 pKa = 4.6

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J4TAN2|J4TAN2_9FIRM Tripeptide aminopeptidase OS=Lachnoanaerobaculum sp. ICM7 OX=936594 GN=pepT PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2588

0

2588

838843

33

3464

324.1

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.615 ± 0.05

1.13 ± 0.02

6.261 ± 0.037

7.409 ± 0.056

4.471 ± 0.039

6.816 ± 0.049

1.44 ± 0.02

8.722 ± 0.062

8.173 ± 0.05

8.646 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.028

5.49 ± 0.046

2.887 ± 0.029

2.197 ± 0.023

3.775 ± 0.03

6.41 ± 0.035

5.064 ± 0.058

6.573 ± 0.039

0.789 ± 0.017

4.285 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski