Natronorubrum tibetense GA33

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natronorubrum; Natronorubrum tibetense

Average proteome isoelectric point is 4.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L9VRY1|L9VRY1_9EURY Basic membrane lipoprotein OS=Natronorubrum tibetense GA33 OX=1114856 GN=C496_13756 PE=4 SV=1
MM1 pKa = 7.31GASDD5 pKa = 4.24TIAAYY10 pKa = 10.27FGFDD14 pKa = 3.18EE15 pKa = 5.59HH16 pKa = 6.12EE17 pKa = 3.78TDD19 pKa = 4.43YY20 pKa = 10.42RR21 pKa = 11.84TEE23 pKa = 3.94TLAGVTTFLAMAYY36 pKa = 9.32IIVVNPAILAEE47 pKa = 4.97AITIDD52 pKa = 4.42GYY54 pKa = 9.96TDD56 pKa = 3.0AQTRR60 pKa = 11.84DD61 pKa = 3.56VIAIATILASVLAIGVMAFWANRR84 pKa = 11.84PFGLAPGMGLNAFFAFTVVIILGVPWEE111 pKa = 4.16LALAAVFVEE120 pKa = 4.81GIIFIALTAVGARR133 pKa = 11.84KK134 pKa = 9.95YY135 pKa = 10.35IIEE138 pKa = 4.79LFPEE142 pKa = 4.31PVKK145 pKa = 10.53FAVGAGIGVFLLFLGLQEE163 pKa = 4.19MEE165 pKa = 4.36VVVGYY170 pKa = 10.12DD171 pKa = 3.22ATLLTLGNVLEE182 pKa = 4.61SPVAALSLTGLALTFFLYY200 pKa = 10.82ARR202 pKa = 11.84GIRR205 pKa = 11.84GSIIIGILSTAVAGWLLTLLDD226 pKa = 4.02VVSPGSLVPEE236 pKa = 4.34PAYY239 pKa = 10.61DD240 pKa = 3.27QATNDD245 pKa = 3.93GLFSMLLSVEE255 pKa = 3.92YY256 pKa = 10.4DD257 pKa = 3.15ITPLISGFIDD267 pKa = 3.91GLGMMADD274 pKa = 4.11DD275 pKa = 4.55PLVFALVVFTFFFVDD290 pKa = 4.8FFDD293 pKa = 3.96TAGTLIGVSQIGGFLDD309 pKa = 4.87DD310 pKa = 5.36EE311 pKa = 5.43GDD313 pKa = 3.51LPEE316 pKa = 4.97IEE318 pKa = 4.57RR319 pKa = 11.84PLMADD324 pKa = 2.96AVGTTFGAMIGTSTVTTYY342 pKa = 10.35IEE344 pKa = 4.22SSTGIEE350 pKa = 3.78EE351 pKa = 4.36GGRR354 pKa = 11.84TGFTALIVGVLFLAALLFVPLMRR377 pKa = 11.84AVPQYY382 pKa = 9.06ATYY385 pKa = 10.05IALVVVGIIMLQGVADD401 pKa = 4.96IDD403 pKa = 3.65WQDD406 pKa = 3.6PVWAISAGLTITIMPLTASIANGLAAGIISYY437 pKa = 9.36PLIKK441 pKa = 10.31AAIGEE446 pKa = 4.25ARR448 pKa = 11.84DD449 pKa = 3.57VSPGQWVLAVLFVGYY464 pKa = 8.81FAIYY468 pKa = 9.22FAVEE472 pKa = 3.93AGQITFF478 pKa = 3.94

Molecular weight: 50.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L9VPD0|L9VPD0_9EURY Uncharacterized protein OS=Natronorubrum tibetense GA33 OX=1114856 GN=C496_16057 PE=3 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.76GEE5 pKa = 3.81KK6 pKa = 10.11AIWIWAPIIAKK17 pKa = 9.69KK18 pKa = 10.4CPKK21 pKa = 9.74CGNSSSYY28 pKa = 10.68HH29 pKa = 5.33EE30 pKa = 5.18RR31 pKa = 11.84SDD33 pKa = 3.87CEE35 pKa = 4.01YY36 pKa = 11.3DD37 pKa = 3.32EE38 pKa = 4.92TEE40 pKa = 3.8PDD42 pKa = 3.25EE43 pKa = 4.38WNKK46 pKa = 10.67GLVGFRR52 pKa = 11.84PAPVFDD58 pKa = 3.97ISQTKK63 pKa = 10.39GEE65 pKa = 4.55SLPEE69 pKa = 4.13LEE71 pKa = 5.08TVLSLPPLRR80 pKa = 11.84EE81 pKa = 3.51LEE83 pKa = 4.09QRR85 pKa = 11.84VDD87 pKa = 3.27RR88 pKa = 11.84HH89 pKa = 5.55SLGVGSSAGRR99 pKa = 11.84RR100 pKa = 11.84LCGCARR106 pKa = 11.84GGHH109 pKa = 5.66LRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84PRR115 pKa = 11.84LRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84DD120 pKa = 3.76PRR122 pKa = 11.84GGAPPSRR129 pKa = 11.84VRR131 pKa = 11.84VPRR134 pKa = 11.84VRR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84GLCSRR143 pKa = 4.63

Molecular weight: 16.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4671

0

4671

1348622

27

3252

288.7

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.171 ± 0.051

0.773 ± 0.011

8.714 ± 0.058

9.268 ± 0.055

3.295 ± 0.025

8.198 ± 0.036

2.048 ± 0.019

4.597 ± 0.032

1.797 ± 0.022

8.867 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.743 ± 0.016

2.407 ± 0.021

4.531 ± 0.024

2.548 ± 0.023

6.301 ± 0.044

5.765 ± 0.029

6.547 ± 0.033

8.547 ± 0.038

1.138 ± 0.014

2.745 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski