Amino acid dipepetide frequency for Natronorubrum tibetense GA33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.671AlaAla: 11.671 ± 0.138	0.727AlaCys: 0.727 ± 0.025	9.434AlaAsp: 9.434 ± 0.107	8.198AlaGlu: 8.198 ± 0.09	3.736AlaPhe: 3.736 ± 0.061	8.532AlaGly: 8.532 ± 0.096	1.758AlaHis: 1.758 ± 0.039	5.48AlaIle: 5.48 ± 0.067	1.745AlaLys: 1.745 ± 0.033	9.666AlaLeu: 9.666 ± 0.109	2.015AlaMet: 2.015 ± 0.044	2.476AlaAsn: 2.476 ± 0.041	3.369AlaPro: 3.369 ± 0.05	2.161AlaGln: 2.161 ± 0.041	5.417AlaArg: 5.417 ± 0.077	5.32AlaSer: 5.32 ± 0.077	6.826AlaThr: 6.826 ± 0.083	9.592AlaVal: 9.592 ± 0.106	0.971AlaTrp: 0.971 ± 0.029	2.612AlaTyr: 2.612 ± 0.049	0.001AlaXaa: 0.001 ± 0.001
Cys	0.604CysAla: 0.604 ± 0.023	0.126CysCys: 0.126 ± 0.011	0.662CysAsp: 0.662 ± 0.023	0.633CysGlu: 0.633 ± 0.024	0.231CysPhe: 0.231 ± 0.012	0.855CysGly: 0.855 ± 0.032	0.209CysHis: 0.209 ± 0.013	0.295CysIle: 0.295 ± 0.016	0.149CysLys: 0.149 ± 0.01	0.636CysLeu: 0.636 ± 0.021	0.111CysMet: 0.111 ± 0.01	0.199CysAsn: 0.199 ± 0.013	0.49CysPro: 0.49 ± 0.021	0.189CysGln: 0.189 ± 0.013	0.518CysArg: 0.518 ± 0.022	0.48CysSer: 0.48 ± 0.019	0.44CysThr: 0.44 ± 0.02	0.52CysVal: 0.52 ± 0.022	0.119CysTrp: 0.119 ± 0.009	0.259CysTyr: 0.259 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	9.459AspAla: 9.459 ± 0.099	0.755AspCys: 0.755 ± 0.026	9.374AspAsp: 9.374 ± 0.155	9.518AspGlu: 9.518 ± 0.126	1.777AspPhe: 1.777 ± 0.044	8.272AspGly: 8.272 ± 0.1	1.998AspHis: 1.998 ± 0.044	3.479AspIle: 3.479 ± 0.056	0.959AspLys: 0.959 ± 0.033	7.148AspLeu: 7.148 ± 0.091	1.201AspMet: 1.201 ± 0.033	1.406AspAsn: 1.406 ± 0.037	4.6AspPro: 4.6 ± 0.072	1.88AspGln: 1.88 ± 0.044	6.614AspArg: 6.614 ± 0.078	4.278AspSer: 4.278 ± 0.063	4.055AspThr: 4.055 ± 0.068	7.467AspVal: 7.467 ± 0.078	1.027AspTrp: 1.027 ± 0.033	1.876AspTyr: 1.876 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.598GluAla: 8.598 ± 0.095	0.617GluCys: 0.617 ± 0.025	6.587GluAsp: 6.587 ± 0.113	7.517GluGlu: 7.517 ± 0.125	3.32GluPhe: 3.32 ± 0.055	5.665GluGly: 5.665 ± 0.077	2.147GluHis: 2.147 ± 0.041	4.739GluIle: 4.739 ± 0.062	1.896GluLys: 1.896 ± 0.044	8.173GluLeu: 8.173 ± 0.095	1.952GluMet: 1.952 ± 0.04	2.629GluAsn: 2.629 ± 0.05	4.502GluPro: 4.502 ± 0.068	3.048GluGln: 3.048 ± 0.064	7.662GluArg: 7.662 ± 0.107	6.102GluSer: 6.102 ± 0.069	7.841GluThr: 7.841 ± 0.108	5.446GluVal: 5.446 ± 0.079	1.18GluTrp: 1.18 ± 0.028	3.054GluTyr: 3.054 ± 0.049	0.004GluXaa: 0.004 ± 0.002
Phe	3.501PheAla: 3.501 ± 0.062	0.299PheCys: 0.299 ± 0.016	3.459PheAsp: 3.459 ± 0.051	3.362PheGlu: 3.362 ± 0.053	1.04PhePhe: 1.04 ± 0.03	3.137PheGly: 3.137 ± 0.054	0.701PheHis: 0.701 ± 0.022	1.097PheIle: 1.097 ± 0.036	0.468PheLys: 0.468 ± 0.021	2.711PheLeu: 2.711 ± 0.058	0.506PheMet: 0.506 ± 0.018	0.687PheAsn: 0.687 ± 0.022	1.332PhePro: 1.332 ± 0.033	0.802PheGln: 0.802 ± 0.025	1.708PheArg: 1.708 ± 0.037	1.679PheSer: 1.679 ± 0.043	1.889PheThr: 1.889 ± 0.04	3.269PheVal: 3.269 ± 0.057	0.406PheTrp: 0.406 ± 0.02	0.894PheTyr: 0.894 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	7.36GlyAla: 7.36 ± 0.099	0.77GlyCys: 0.77 ± 0.027	6.798GlyAsp: 6.798 ± 0.085	7.07GlyGlu: 7.07 ± 0.085	3.068GlyPhe: 3.068 ± 0.056	6.719GlyGly: 6.719 ± 0.104	1.691GlyHis: 1.691 ± 0.039	4.541GlyIle: 4.541 ± 0.07	1.767GlyLys: 1.767 ± 0.043	7.25GlyLeu: 7.25 ± 0.092	1.61GlyMet: 1.61 ± 0.038	1.975GlyAsn: 1.975 ± 0.041	3.119GlyPro: 3.119 ± 0.054	2.018GlyGln: 2.018 ± 0.04	4.532GlyArg: 4.532 ± 0.06	5.218GlySer: 5.218 ± 0.073	5.758GlyThr: 5.758 ± 0.07	6.862GlyVal: 6.862 ± 0.077	1.165GlyTrp: 1.165 ± 0.032	2.683GlyTyr: 2.683 ± 0.041	0.002GlyXaa: 0.002 ± 0.001
His	2.025HisAla: 2.025 ± 0.042	0.226HisCys: 0.226 ± 0.014	1.955HisAsp: 1.955 ± 0.046	1.819HisGlu: 1.819 ± 0.04	0.561HisPhe: 0.561 ± 0.023	2.041HisGly: 2.041 ± 0.04	0.541HisHis: 0.541 ± 0.023	0.727HisIle: 0.727 ± 0.027	0.301HisLys: 0.301 ± 0.015	1.722HisLeu: 1.722 ± 0.038	0.306HisMet: 0.306 ± 0.015	0.462HisAsn: 0.462 ± 0.02	1.223HisPro: 1.223 ± 0.031	0.513HisGln: 0.513 ± 0.019	1.356HisArg: 1.356 ± 0.031	0.957HisSer: 0.957 ± 0.027	1.079HisThr: 1.079 ± 0.029	1.861HisVal: 1.861 ± 0.038	0.243HisTrp: 0.243 ± 0.014	0.561HisTyr: 0.561 ± 0.018	0.001HisXaa: 0.001 ± 0.001
Ile	5.274IleAla: 5.274 ± 0.071	0.323IleCys: 0.323 ± 0.015	4.934IleAsp: 4.934 ± 0.063	4.752IleGlu: 4.752 ± 0.073	1.12IlePhe: 1.12 ± 0.036	4.342IleGly: 4.342 ± 0.069	0.909IleHis: 0.909 ± 0.028	1.573IleIle: 1.573 ± 0.044	0.707IleLys: 0.707 ± 0.024	3.274IleLeu: 3.274 ± 0.063	0.549IleMet: 0.549 ± 0.023	1.023IleAsn: 1.023 ± 0.029	2.201IlePro: 2.201 ± 0.039	1.162IleGln: 1.162 ± 0.032	2.755IleArg: 2.755 ± 0.047	2.442IleSer: 2.442 ± 0.047	2.724IleThr: 2.724 ± 0.048	4.456IleVal: 4.456 ± 0.075	0.397IleTrp: 0.397 ± 0.016	1.049IleTyr: 1.049 ± 0.028	0.004IleXaa: 0.004 ± 0.002
Lys	1.66LysAla: 1.66 ± 0.036	0.126LysCys: 0.126 ± 0.011	1.029LysAsp: 1.029 ± 0.032	1.272LysGlu: 1.272 ± 0.034	0.469LysPhe: 0.469 ± 0.019	1.295LysGly: 1.295 ± 0.033	0.523LysHis: 0.523 ± 0.018	0.832LysIle: 0.832 ± 0.027	0.525LysLys: 0.525 ± 0.02	1.66LysLeu: 1.66 ± 0.037	0.358LysMet: 0.358 ± 0.015	0.543LysAsn: 0.543 ± 0.023	0.964LysPro: 0.964 ± 0.028	0.706LysGln: 0.706 ± 0.026	1.641LysArg: 1.641 ± 0.039	1.173LysSer: 1.173 ± 0.034	1.356LysThr: 1.356 ± 0.035	1.085LysVal: 1.085 ± 0.033	0.21LysTrp: 0.21 ± 0.012	0.544LysTyr: 0.544 ± 0.02	0.001LysXaa: 0.001 ± 0.001
Leu	10.067LeuAla: 10.067 ± 0.117	0.671LeuCys: 0.671 ± 0.023	6.085LeuAsp: 6.085 ± 0.082	11.676LeuGlu: 11.676 ± 0.123	3.083LeuPhe: 3.083 ± 0.055	7.081LeuGly: 7.081 ± 0.083	1.457LeuHis: 1.457 ± 0.035	3.266LeuIle: 3.266 ± 0.064	1.446LeuLys: 1.446 ± 0.04	8.488LeuLeu: 8.488 ± 0.122	1.238LeuMet: 1.238 ± 0.033	1.751LeuAsn: 1.751 ± 0.04	3.837LeuPro: 3.837 ± 0.057	2.093LeuGln: 2.093 ± 0.042	4.822LeuArg: 4.822 ± 0.063	5.507LeuSer: 5.507 ± 0.069	5.222LeuThr: 5.222 ± 0.068	7.824LeuVal: 7.824 ± 0.108	0.858LeuTrp: 0.858 ± 0.026	2.194LeuTyr: 2.194 ± 0.042	0.001LeuXaa: 0.001 ± 0.001
Met	1.771MetAla: 1.771 ± 0.039	0.116MetCys: 0.116 ± 0.009	1.301MetAsp: 1.301 ± 0.033	1.106MetGlu: 1.106 ± 0.03	0.526MetPhe: 0.526 ± 0.023	1.423MetGly: 1.423 ± 0.036	0.34MetHis: 0.34 ± 0.016	0.826MetIle: 0.826 ± 0.028	0.43MetLys: 0.43 ± 0.021	1.528MetLeu: 1.528 ± 0.036	0.305MetMet: 0.305 ± 0.014	0.661MetAsn: 0.661 ± 0.021	0.776MetPro: 0.776 ± 0.028	0.515MetGln: 0.515 ± 0.023	0.993MetArg: 0.993 ± 0.027	1.457MetSer: 1.457 ± 0.034	1.503MetThr: 1.503 ± 0.031	1.241MetVal: 1.241 ± 0.033	0.179MetTrp: 0.179 ± 0.011	0.43MetTyr: 0.43 ± 0.018	0.001MetXaa: 0.001 ± 0.001
Asn	2.632AsnAla: 2.632 ± 0.046	0.235AsnCys: 0.235 ± 0.015	1.808AsnAsp: 1.808 ± 0.036	2.098AsnGlu: 2.098 ± 0.041	0.66AsnPhe: 0.66 ± 0.023	2.213AsnGly: 2.213 ± 0.048	0.545AsnHis: 0.545 ± 0.022	0.96AsnIle: 0.96 ± 0.033	0.432AsnLys: 0.432 ± 0.02	1.89AsnLeu: 1.89 ± 0.036	0.449AsnMet: 0.449 ± 0.017	0.547AsnAsn: 0.547 ± 0.023	1.504AsnPro: 1.504 ± 0.034	0.631AsnGln: 0.631 ± 0.021	1.747AsnArg: 1.747 ± 0.038	1.086AsnSer: 1.086 ± 0.029	1.291AsnThr: 1.291 ± 0.043	2.26AsnVal: 2.26 ± 0.04	0.379AsnTrp: 0.379 ± 0.017	0.696AsnTyr: 0.696 ± 0.027	0.001AsnXaa: 0.001 ± 0.001
Pro	4.231ProAla: 4.231 ± 0.059	0.245ProCys: 0.245 ± 0.012	4.845ProAsp: 4.845 ± 0.073	4.512ProGlu: 4.512 ± 0.086	1.585ProPhe: 1.585 ± 0.036	3.457ProGly: 3.457 ± 0.051	0.935ProHis: 0.935 ± 0.025	2.242ProIle: 2.242 ± 0.043	0.878ProLys: 0.878 ± 0.029	3.561ProLeu: 3.561 ± 0.06	0.879ProMet: 0.879 ± 0.026	1.197ProAsn: 1.197 ± 0.028	2.071ProPro: 2.071 ± 0.048	0.956ProGln: 0.956 ± 0.026	2.195ProArg: 2.195 ± 0.046	2.746ProSer: 2.746 ± 0.047	3.258ProThr: 3.258 ± 0.047	3.885ProVal: 3.885 ± 0.053	0.498ProTrp: 0.498 ± 0.019	1.137ProTyr: 1.137 ± 0.026	0.001ProXaa: 0.001 ± 0.001
Gln	2.334GlnAla: 2.334 ± 0.051	0.171GlnCys: 0.171 ± 0.01	1.444GlnAsp: 1.444 ± 0.031	1.891GlnGlu: 1.891 ± 0.047	1.135GlnPhe: 1.135 ± 0.03	1.599GlnGly: 1.599 ± 0.035	0.575GlnHis: 0.575 ± 0.024	1.223GlnIle: 1.223 ± 0.033	0.574GlnLys: 0.574 ± 0.023	2.6GlnLeu: 2.6 ± 0.04	0.521GlnMet: 0.521 ± 0.022	0.721GlnAsn: 0.721 ± 0.024	1.281GlnPro: 1.281 ± 0.036	1.116GlnGln: 1.116 ± 0.035	1.993GlnArg: 1.993 ± 0.042	1.686GlnSer: 1.686 ± 0.033	1.623GlnThr: 1.623 ± 0.038	1.72GlnVal: 1.72 ± 0.041	0.349GlnTrp: 0.349 ± 0.014	0.926GlnTyr: 0.926 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.704ArgAla: 5.704 ± 0.078	0.469ArgCys: 0.469 ± 0.019	4.915ArgAsp: 4.915 ± 0.054	6.44ArgGlu: 6.44 ± 0.09	2.296ArgPhe: 2.296 ± 0.043	4.127ArgGly: 4.127 ± 0.061	1.231ArgHis: 1.231 ± 0.033	3.185ArgIle: 3.185 ± 0.055	1.413ArgLys: 1.413 ± 0.034	5.91ArgLeu: 5.91 ± 0.08	1.243ArgMet: 1.243 ± 0.034	1.697ArgAsn: 1.697 ± 0.036	2.509ArgPro: 2.509 ± 0.052	1.762ArgGln: 1.762 ± 0.037	4.649ArgArg: 4.649 ± 0.07	3.778ArgSer: 3.778 ± 0.06	4.227ArgThr: 4.227 ± 0.066	4.754ArgVal: 4.754 ± 0.064	0.782ArgTrp: 0.782 ± 0.022	1.912ArgTyr: 1.912 ± 0.041	0.001ArgXaa: 0.001 ± 0.001
Ser	5.227SerAla: 5.227 ± 0.075	0.373SerCys: 0.373 ± 0.018	5.0SerAsp: 5.0 ± 0.07	4.777SerGlu: 4.777 ± 0.071	1.938SerPhe: 1.938 ± 0.041	5.148SerGly: 5.148 ± 0.074	1.106SerHis: 1.106 ± 0.027	2.926SerIle: 2.926 ± 0.052	1.203SerLys: 1.203 ± 0.032	5.024SerLeu: 5.024 ± 0.054	1.195SerMet: 1.195 ± 0.028	1.56SerAsn: 1.56 ± 0.041	2.623SerPro: 2.623 ± 0.046	1.445SerGln: 1.445 ± 0.04	3.432SerArg: 3.432 ± 0.06	3.687SerSer: 3.687 ± 0.058	3.787SerThr: 3.787 ± 0.054	5.027SerVal: 5.027 ± 0.065	0.612SerTrp: 0.612 ± 0.022	1.555SerTyr: 1.555 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	6.877ThrAla: 6.877 ± 0.066	0.42ThrCys: 0.42 ± 0.017	6.151ThrAsp: 6.151 ± 0.069	5.087ThrGlu: 5.087 ± 0.064	2.19ThrPhe: 2.19 ± 0.047	5.628ThrGly: 5.628 ± 0.068	1.241ThrHis: 1.241 ± 0.034	3.585ThrIle: 3.585 ± 0.057	1.077ThrLys: 1.077 ± 0.028	5.839ThrLeu: 5.839 ± 0.064	1.128ThrMet: 1.128 ± 0.026	1.607ThrAsn: 1.607 ± 0.036	3.098ThrPro: 3.098 ± 0.047	1.439ThrGln: 1.439 ± 0.037	3.219ThrArg: 3.219 ± 0.057	3.144ThrSer: 3.144 ± 0.049	4.349ThrThr: 4.349 ± 0.066	7.023ThrVal: 7.023 ± 0.108	0.643ThrTrp: 0.643 ± 0.022	1.731ThrTyr: 1.731 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	9.093ValAla: 9.093 ± 0.116	0.687ValCys: 0.687 ± 0.027	7.829ValAsp: 7.829 ± 0.085	7.381ValGlu: 7.381 ± 0.08	2.852ValPhe: 2.852 ± 0.059	7.233ValGly: 7.233 ± 0.087	1.625ValHis: 1.625 ± 0.036	3.55ValIle: 3.55 ± 0.058	1.266ValLys: 1.266 ± 0.033	7.835ValLeu: 7.835 ± 0.091	1.261ValMet: 1.261 ± 0.034	1.862ValAsn: 1.862 ± 0.041	4.106ValPro: 4.106 ± 0.053	1.907ValGln: 1.907 ± 0.041	5.056ValArg: 5.056 ± 0.067	5.036ValSer: 5.036 ± 0.071	5.91ValThr: 5.91 ± 0.085	8.099ValVal: 8.099 ± 0.099	0.782ValTrp: 0.782 ± 0.027	2.098ValTyr: 2.098 ± 0.04	0.001ValXaa: 0.001 ± 0.001
Trp	0.89TrpAla: 0.89 ± 0.024	0.098TrpCys: 0.098 ± 0.009	0.784TrpAsp: 0.784 ± 0.027	0.888TrpGlu: 0.888 ± 0.026	0.477TrpPhe: 0.477 ± 0.019	0.849TrpGly: 0.849 ± 0.026	0.277TrpHis: 0.277 ± 0.014	0.613TrpIle: 0.613 ± 0.02	0.299TrpLys: 0.299 ± 0.017	1.256TrpLeu: 1.256 ± 0.03	0.228TrpMet: 0.228 ± 0.013	0.406TrpAsn: 0.406 ± 0.018	0.418TrpPro: 0.418 ± 0.017	0.371TrpGln: 0.371 ± 0.015	0.766TrpArg: 0.766 ± 0.024	0.631TrpSer: 0.631 ± 0.023	0.77TrpThr: 0.77 ± 0.024	0.787TrpVal: 0.787 ± 0.023	0.205TrpTrp: 0.205 ± 0.012	0.365TrpTyr: 0.365 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.731TyrAla: 2.731 ± 0.045	0.274TyrCys: 0.274 ± 0.014	2.747TyrAsp: 2.747 ± 0.05	2.679TyrGlu: 2.679 ± 0.047	0.882TyrPhe: 0.882 ± 0.024	2.358TyrGly: 2.358 ± 0.04	0.669TyrHis: 0.669 ± 0.024	0.83TyrIle: 0.83 ± 0.03	0.437TyrLys: 0.437 ± 0.019	2.493TyrLeu: 2.493 ± 0.043	0.374TyrMet: 0.374 ± 0.015	0.658TyrAsn: 0.658 ± 0.025	1.286TyrPro: 1.286 ± 0.034	0.767TyrGln: 0.767 ± 0.024	1.927TyrArg: 1.927 ± 0.043	1.239TyrSer: 1.239 ± 0.029	1.566TyrThr: 1.566 ± 0.036	2.288TyrVal: 2.288 ± 0.039	0.373TyrTrp: 0.373 ± 0.02	0.874TyrTyr: 0.874 ± 0.025	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.002	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.003XaaArg: 0.003 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4671 proteins (1348622 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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