Hubei tombus-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGN2|A0A1L3KGN2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 6 OX=1923293 PE=4 SV=1
MM1 pKa = 7.38TSVKK5 pKa = 10.64DD6 pKa = 3.55SLRR9 pKa = 11.84KK10 pKa = 9.29FLPSTAPISHH20 pKa = 7.34DD21 pKa = 3.39AFVSGYY27 pKa = 10.02KK28 pKa = 9.94GRR30 pKa = 11.84KK31 pKa = 7.76KK32 pKa = 10.39EE33 pKa = 3.95LYY35 pKa = 9.81QRR37 pKa = 11.84ALDD40 pKa = 4.31EE41 pKa = 4.32IRR43 pKa = 11.84ATEE46 pKa = 4.13SNLEE50 pKa = 3.77NDD52 pKa = 2.77SRR54 pKa = 11.84LNVFVKK60 pKa = 10.46FEE62 pKa = 4.31KK63 pKa = 9.94TDD65 pKa = 3.25HH66 pKa = 5.27TTKK69 pKa = 10.43KK70 pKa = 10.61DD71 pKa = 3.31PVPRR75 pKa = 11.84VISPRR80 pKa = 11.84DD81 pKa = 3.17PKK83 pKa = 11.03FNIRR87 pKa = 11.84VGRR90 pKa = 11.84YY91 pKa = 7.85LKK93 pKa = 10.35PLEE96 pKa = 3.97EE97 pKa = 4.56RR98 pKa = 11.84LFKK101 pKa = 11.1SLGKK105 pKa = 10.59LFGHH109 pKa = 5.69KK110 pKa = 8.79TVLKK114 pKa = 10.0GVNSQRR120 pKa = 11.84SAEE123 pKa = 3.97LLKK126 pKa = 10.69EE127 pKa = 3.6KK128 pKa = 10.41WEE130 pKa = 4.17MYY132 pKa = 9.84RR133 pKa = 11.84NPVAIGLDD141 pKa = 3.21ASRR144 pKa = 11.84FDD146 pKa = 3.34QHH148 pKa = 8.13VSIDD152 pKa = 3.5ALRR155 pKa = 11.84WEE157 pKa = 4.0HH158 pKa = 7.12SIYY161 pKa = 10.83LEE163 pKa = 4.16CFPQRR168 pKa = 11.84KK169 pKa = 7.59HH170 pKa = 5.33QEE172 pKa = 3.57RR173 pKa = 11.84LRR175 pKa = 11.84RR176 pKa = 11.84LLKK179 pKa = 10.44LQEE182 pKa = 4.56LNSCTGYY189 pKa = 9.13TPDD192 pKa = 5.1GKK194 pKa = 10.79LKK196 pKa = 10.81YY197 pKa = 8.99NVKK200 pKa = 7.88GTRR203 pKa = 11.84MSGDD207 pKa = 3.37MNTSLGNCVLMCSMIKK223 pKa = 10.19AYY225 pKa = 10.77LMSKK229 pKa = 10.6GIDD232 pKa = 3.24GQLANNGDD240 pKa = 3.88DD241 pKa = 3.47CVVFMEE247 pKa = 5.83KK248 pKa = 10.31SDD250 pKa = 4.23LLNFSDD256 pKa = 5.87GLYY259 pKa = 10.85DD260 pKa = 3.33WFLALGFNMAIEE272 pKa = 4.19EE273 pKa = 4.26PVFEE277 pKa = 4.23FEE279 pKa = 5.54QIEE282 pKa = 4.56FCQTKK287 pKa = 9.6PVFDD291 pKa = 3.71GEE293 pKa = 4.2YY294 pKa = 9.9WIMCRR299 pKa = 11.84NPHH302 pKa = 4.89TAINKK307 pKa = 9.47DD308 pKa = 3.4SVMLKK313 pKa = 10.24PFSCSSEE320 pKa = 3.6FRR322 pKa = 11.84GWLDD326 pKa = 3.33AVGTGGLAMAGGIPIFQEE344 pKa = 4.83LYY346 pKa = 9.16RR347 pKa = 11.84TYY349 pKa = 10.53QKK351 pKa = 10.62CGQQRR356 pKa = 11.84KK357 pKa = 9.04ISEE360 pKa = 4.62DD361 pKa = 3.43LLPWSFTNLSKK372 pKa = 11.06GMTRR376 pKa = 11.84QYY378 pKa = 10.57GTVSPAARR386 pKa = 11.84ASFYY390 pKa = 10.01WAFDD394 pKa = 3.26ITPDD398 pKa = 3.56EE399 pKa = 4.4QIALEE404 pKa = 4.78DD405 pKa = 4.47YY406 pKa = 10.67YY407 pKa = 11.63SSTKK411 pKa = 9.97IVSSLGPYY419 pKa = 8.85EE420 pKa = 3.91PRR422 pKa = 11.84GVFAEE427 pKa = 4.2

Molecular weight: 48.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGN2|A0A1L3KGN2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 6 OX=1923293 PE=4 SV=1
MM1 pKa = 7.32AVKK4 pKa = 10.55GEE6 pKa = 3.9KK7 pKa = 9.83RR8 pKa = 11.84VIKK11 pKa = 9.55EE12 pKa = 3.54GKK14 pKa = 9.02VFVEE18 pKa = 3.61QRR20 pKa = 11.84YY21 pKa = 9.19NGKK24 pKa = 9.2RR25 pKa = 11.84WVSEE29 pKa = 4.15SSWVSGQQPSKK40 pKa = 10.96EE41 pKa = 4.04GGLTKK46 pKa = 10.49SVARR50 pKa = 11.84KK51 pKa = 9.31IRR53 pKa = 11.84GGVSRR58 pKa = 11.84AGGFVTAPVSGAMITRR74 pKa = 11.84PTVPRR79 pKa = 11.84FGMRR83 pKa = 11.84GDD85 pKa = 3.52STIVSNSEE93 pKa = 3.6LVLNLTPIALAFGVQSLPLIAAQPAWLSTIADD125 pKa = 4.14NYY127 pKa = 10.54SKK129 pKa = 10.26WRR131 pKa = 11.84WISMRR136 pKa = 11.84LIYY139 pKa = 10.34SPKK142 pKa = 10.34CPTTTSGTVAMCLSYY157 pKa = 11.01DD158 pKa = 4.27RR159 pKa = 11.84NDD161 pKa = 3.66APPGSRR167 pKa = 11.84VQLSQTYY174 pKa = 9.63KK175 pKa = 10.79AINFPPYY182 pKa = 10.38AGYY185 pKa = 10.72DD186 pKa = 3.29GAAILNTDD194 pKa = 3.63VTPTSAIYY202 pKa = 10.77LDD204 pKa = 3.69VDD206 pKa = 3.36VTRR209 pKa = 11.84FDD211 pKa = 4.06KK212 pKa = 10.95NWYY215 pKa = 7.69STIGTVAFAALTAFDD230 pKa = 4.16QNQFCPATVHH240 pKa = 6.29IGSDD244 pKa = 3.44GGPAVAVPPGDD255 pKa = 4.55LFFKK259 pKa = 10.7YY260 pKa = 10.28VIEE263 pKa = 4.85LIEE266 pKa = 4.91PINPTMNVV274 pKa = 2.92

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

902

201

427

300.7

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.874 ± 1.112

1.663 ± 0.285

5.432 ± 0.427

5.322 ± 0.998

5.1 ± 0.445

6.763 ± 0.846

0.998 ± 0.395

4.878 ± 0.544

7.095 ± 1.042

8.204 ± 1.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.993 ± 0.387

3.991 ± 0.01

5.322 ± 0.811

3.437 ± 0.063

5.765 ± 0.27

7.206 ± 1.067

5.987 ± 0.838

7.317 ± 1.335

1.885 ± 0.156

3.769 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski