Vibrio phage pVa-21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6L1Z1|A0A1S6L1Z1_9CAUD Uncharacterized protein OS=Vibrio phage pVa-21 OX=1958921 GN=pVa21_168 PE=4 SV=1
MM1 pKa = 7.24YY2 pKa = 10.2DD3 pKa = 3.17NYY5 pKa = 10.85GFQYY9 pKa = 10.65LPRR12 pKa = 11.84EE13 pKa = 3.95LVNRR17 pKa = 11.84YY18 pKa = 9.9DD19 pKa = 3.66EE20 pKa = 4.65IYY22 pKa = 10.55RR23 pKa = 11.84SILSEE28 pKa = 4.11VVHH31 pKa = 6.65NLVQHH36 pKa = 6.59DD37 pKa = 4.07LGHH40 pKa = 5.79FRR42 pKa = 11.84SYY44 pKa = 11.12LSCLDD49 pKa = 3.69YY50 pKa = 10.37ATWIYY55 pKa = 10.05WLEE58 pKa = 4.19SANPATLEE66 pKa = 3.95QLEE69 pKa = 4.43VAAKK73 pKa = 9.13YY74 pKa = 10.8GSDD77 pKa = 2.87IAEE80 pKa = 4.53TIRR83 pKa = 11.84DD84 pKa = 3.77EE85 pKa = 4.28VHH87 pKa = 7.31DD88 pKa = 4.58AFEE91 pKa = 4.5NSDD94 pKa = 3.89GEE96 pKa = 4.25CDD98 pKa = 3.43EE99 pKa = 4.45EE100 pKa = 5.09QEE102 pKa = 4.33LDD104 pKa = 3.8YY105 pKa = 11.54AVGNSVLLVMPTTNEE120 pKa = 3.48FNEE123 pKa = 4.4FEE125 pKa = 4.26RR126 pKa = 11.84CVWDD130 pKa = 5.87DD131 pKa = 3.46IQDD134 pKa = 3.78SNVIEE139 pKa = 4.75PYY141 pKa = 10.75FNGDD145 pKa = 3.66LLVSIRR151 pKa = 11.84PGLSRR156 pKa = 11.84LLLTSNSGMYY166 pKa = 8.05VTT168 pKa = 5.54

Molecular weight: 19.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6L1S7|A0A1S6L1S7_9CAUD Uncharacterized protein OS=Vibrio phage pVa-21 OX=1958921 GN=pVa21_099 PE=4 SV=1
MM1 pKa = 7.58SLTDD5 pKa = 3.66LVVARR10 pKa = 11.84KK11 pKa = 10.04DD12 pKa = 3.76SIVGFTKK19 pKa = 10.6GASITDD25 pKa = 3.28NKK27 pKa = 10.47LRR29 pKa = 11.84ILIQPTGLYY38 pKa = 10.47GHH40 pKa = 8.0LLTSKK45 pKa = 10.03HH46 pKa = 6.36VSIKK50 pKa = 9.97WSLGDD55 pKa = 3.51RR56 pKa = 11.84KK57 pKa = 10.53SKK59 pKa = 10.79YY60 pKa = 9.69KK61 pKa = 10.0SLRR64 pKa = 11.84YY65 pKa = 9.81LIMEE69 pKa = 4.62RR70 pKa = 11.84VRR72 pKa = 11.84IQQ74 pKa = 2.81

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

69916

43

2329

290.1

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.91 ± 0.164

0.95 ± 0.053

7.212 ± 0.135

7.39 ± 0.141

3.888 ± 0.082

6.006 ± 0.151

2.374 ± 0.078

6.039 ± 0.11

6.054 ± 0.15

8.386 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.988 ± 0.077

5.086 ± 0.1

3.827 ± 0.081

3.184 ± 0.084

5.631 ± 0.106

5.874 ± 0.116

5.937 ± 0.113

7.021 ± 0.094

1.209 ± 0.045

4.035 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski