Amino acid dipepetide frequency for Vibrio phage pVa-21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.55AlaAla: 5.55 ± 0.367
0.601AlaCys: 0.601 ± 0.101
4.534AlaAsp: 4.534 ± 0.283
5.278AlaGlu: 5.278 ± 0.324
2.632AlaPhe: 2.632 ± 0.201
4.048AlaGly: 4.048 ± 0.263
1.602AlaHis: 1.602 ± 0.151
4.32AlaIle: 4.32 ± 0.244
4.92AlaLys: 4.92 ± 0.357
6.007AlaLeu: 6.007 ± 0.319
2.675AlaMet: 2.675 ± 0.218
3.776AlaAsn: 3.776 ± 0.289
2.389AlaPro: 2.389 ± 0.192
2.002AlaGln: 2.002 ± 0.163
3.19AlaArg: 3.19 ± 0.271
3.805AlaSer: 3.805 ± 0.242
4.062AlaThr: 4.062 ± 0.299
4.977AlaVal: 4.977 ± 0.276
0.586AlaTrp: 0.586 ± 0.088
2.145AlaTyr: 2.145 ± 0.166
0.0AlaXaa: 0.0 ± 0.0
Cys
0.501CysAla: 0.501 ± 0.076
0.114CysCys: 0.114 ± 0.039
0.758CysAsp: 0.758 ± 0.124
0.858CysGlu: 0.858 ± 0.132
0.343CysPhe: 0.343 ± 0.074
0.586CysGly: 0.586 ± 0.09
0.243CysHis: 0.243 ± 0.058
0.658CysIle: 0.658 ± 0.087
0.586CysLys: 0.586 ± 0.088
0.701CysLeu: 0.701 ± 0.09
0.343CysMet: 0.343 ± 0.068
0.415CysAsn: 0.415 ± 0.084
0.3CysPro: 0.3 ± 0.056
0.386CysGln: 0.386 ± 0.069
0.629CysArg: 0.629 ± 0.088
0.501CysSer: 0.501 ± 0.076
0.544CysThr: 0.544 ± 0.093
0.672CysVal: 0.672 ± 0.098
0.043CysTrp: 0.043 ± 0.026
0.315CysTyr: 0.315 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
4.877AspAla: 4.877 ± 0.242
0.544AspCys: 0.544 ± 0.099
4.935AspAsp: 4.935 ± 0.385
5.75AspGlu: 5.75 ± 0.285
3.032AspPhe: 3.032 ± 0.199
5.449AspGly: 5.449 ± 0.292
1.23AspHis: 1.23 ± 0.116
4.834AspIle: 4.834 ± 0.241
4.234AspLys: 4.234 ± 0.25
6.365AspLeu: 6.365 ± 0.306
2.331AspMet: 2.331 ± 0.221
3.561AspAsn: 3.561 ± 0.204
2.846AspPro: 2.846 ± 0.182
1.988AspGln: 1.988 ± 0.154
3.79AspArg: 3.79 ± 0.277
3.418AspSer: 3.418 ± 0.237
3.991AspThr: 3.991 ± 0.248
4.863AspVal: 4.863 ± 0.263
1.101AspTrp: 1.101 ± 0.126
2.975AspTyr: 2.975 ± 0.22
0.0AspXaa: 0.0 ± 0.0
Glu
4.935GluAla: 4.935 ± 0.282
0.758GluCys: 0.758 ± 0.117
4.92GluAsp: 4.92 ± 0.261
5.292GluGlu: 5.292 ± 0.296
3.275GluPhe: 3.275 ± 0.225
3.933GluGly: 3.933 ± 0.283
1.945GluHis: 1.945 ± 0.188
4.377GluIle: 4.377 ± 0.25
4.205GluLys: 4.205 ± 0.244
8.067GluLeu: 8.067 ± 0.413
2.474GluMet: 2.474 ± 0.184
3.132GluAsn: 3.132 ± 0.19
2.002GluPro: 2.002 ± 0.176
3.204GluGln: 3.204 ± 0.247
4.305GluArg: 4.305 ± 0.277
3.876GluSer: 3.876 ± 0.263
3.905GluThr: 3.905 ± 0.213
5.693GluVal: 5.693 ± 0.322
0.872GluTrp: 0.872 ± 0.108
2.732GluTyr: 2.732 ± 0.219
0.0GluXaa: 0.0 ± 0.0
Phe
2.56PheAla: 2.56 ± 0.175
0.472PheCys: 0.472 ± 0.081
3.533PheAsp: 3.533 ± 0.213
3.118PheGlu: 3.118 ± 0.214
1.159PhePhe: 1.159 ± 0.099
2.789PheGly: 2.789 ± 0.197
0.815PheHis: 0.815 ± 0.106
2.017PheIle: 2.017 ± 0.171
2.617PheLys: 2.617 ± 0.218
2.432PheLeu: 2.432 ± 0.191
0.887PheMet: 0.887 ± 0.116
2.546PheAsn: 2.546 ± 0.208
1.359PhePro: 1.359 ± 0.156
0.987PheGln: 0.987 ± 0.111
1.931PheArg: 1.931 ± 0.144
2.303PheSer: 2.303 ± 0.18
2.961PheThr: 2.961 ± 0.209
2.188PheVal: 2.188 ± 0.164
0.486PheTrp: 0.486 ± 0.084
1.716PheTyr: 1.716 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
3.318GlyAla: 3.318 ± 0.315
0.658GlyCys: 0.658 ± 0.111
3.948GlyAsp: 3.948 ± 0.26
4.134GlyGlu: 4.134 ± 0.244
2.56GlyPhe: 2.56 ± 0.19
4.405GlyGly: 4.405 ± 0.431
1.459GlyHis: 1.459 ± 0.161
3.719GlyIle: 3.719 ± 0.262
4.162GlyLys: 4.162 ± 0.289
4.749GlyLeu: 4.749 ± 0.262
1.816GlyMet: 1.816 ± 0.16
2.975GlyAsn: 2.975 ± 0.23
1.101GlyPro: 1.101 ± 0.14
1.702GlyGln: 1.702 ± 0.154
3.662GlyArg: 3.662 ± 0.266
3.948GlySer: 3.948 ± 0.279
3.79GlyThr: 3.79 ± 0.246
4.262GlyVal: 4.262 ± 0.269
0.958GlyTrp: 0.958 ± 0.124
2.732GlyTyr: 2.732 ± 0.195
0.0GlyXaa: 0.0 ± 0.0
His
1.573HisAla: 1.573 ± 0.148
0.286HisCys: 0.286 ± 0.07
1.502HisAsp: 1.502 ± 0.172
1.53HisGlu: 1.53 ± 0.149
0.973HisPhe: 0.973 ± 0.097
1.259HisGly: 1.259 ± 0.132
0.486HisHis: 0.486 ± 0.083
1.588HisIle: 1.588 ± 0.159
1.144HisLys: 1.144 ± 0.117
2.117HisLeu: 2.117 ± 0.196
0.601HisMet: 0.601 ± 0.082
1.216HisAsn: 1.216 ± 0.161
1.287HisPro: 1.287 ± 0.16
0.973HisGln: 0.973 ± 0.12
1.244HisArg: 1.244 ± 0.14
1.23HisSer: 1.23 ± 0.163
1.373HisThr: 1.373 ± 0.191
1.716HisVal: 1.716 ± 0.169
0.329HisTrp: 0.329 ± 0.066
1.316HisTyr: 1.316 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
3.962IleAla: 3.962 ± 0.256
0.486IleCys: 0.486 ± 0.088
5.421IleAsp: 5.421 ± 0.313
4.591IleGlu: 4.591 ± 0.298
1.788IlePhe: 1.788 ± 0.175
3.104IleGly: 3.104 ± 0.209
1.645IleHis: 1.645 ± 0.158
3.204IleIle: 3.204 ± 0.229
3.805IleLys: 3.805 ± 0.249
3.89IleLeu: 3.89 ± 0.214
1.53IleMet: 1.53 ± 0.159
3.747IleAsn: 3.747 ± 0.232
3.047IlePro: 3.047 ± 0.219
1.945IleGln: 1.945 ± 0.176
3.619IleArg: 3.619 ± 0.219
3.79IleSer: 3.79 ± 0.25
4.205IleThr: 4.205 ± 0.27
3.919IleVal: 3.919 ± 0.252
0.515IleTrp: 0.515 ± 0.083
2.174IleTyr: 2.174 ± 0.162
0.0IleXaa: 0.0 ± 0.0
Lys
4.649LysAla: 4.649 ± 0.318
0.544LysCys: 0.544 ± 0.082
4.219LysAsp: 4.219 ± 0.233
4.706LysGlu: 4.706 ± 0.277
2.117LysPhe: 2.117 ± 0.186
3.576LysGly: 3.576 ± 0.251
1.788LysHis: 1.788 ± 0.177
2.904LysIle: 2.904 ± 0.188
3.519LysLys: 3.519 ± 0.265
5.464LysLeu: 5.464 ± 0.322
1.859LysMet: 1.859 ± 0.156
2.56LysAsn: 2.56 ± 0.185
2.603LysPro: 2.603 ± 0.203
2.217LysGln: 2.217 ± 0.19
3.418LysArg: 3.418 ± 0.215
3.418LysSer: 3.418 ± 0.262
3.29LysThr: 3.29 ± 0.211
4.033LysVal: 4.033 ± 0.217
0.958LysTrp: 0.958 ± 0.114
2.703LysTyr: 2.703 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
6.265LeuAla: 6.265 ± 0.346
0.687LeuCys: 0.687 ± 0.099
6.708LeuAsp: 6.708 ± 0.33
6.451LeuGlu: 6.451 ± 0.336
3.132LeuPhe: 3.132 ± 0.241
4.734LeuGly: 4.734 ± 0.279
2.031LeuHis: 2.031 ± 0.187
4.834LeuIle: 4.834 ± 0.32
5.106LeuLys: 5.106 ± 0.249
6.022LeuLeu: 6.022 ± 0.265
2.145LeuMet: 2.145 ± 0.187
4.563LeuAsn: 4.563 ± 0.304
3.747LeuPro: 3.747 ± 0.203
2.546LeuGln: 2.546 ± 0.198
4.491LeuArg: 4.491 ± 0.255
6.165LeuSer: 6.165 ± 0.304
5.492LeuThr: 5.492 ± 0.361
5.163LeuVal: 5.163 ± 0.262
0.858LeuTrp: 0.858 ± 0.135
2.718LeuTyr: 2.718 ± 0.154
0.0LeuXaa: 0.0 ± 0.0
Met
2.732MetAla: 2.732 ± 0.253
0.358MetCys: 0.358 ± 0.074
1.931MetAsp: 1.931 ± 0.182
2.288MetGlu: 2.288 ± 0.208
1.173MetPhe: 1.173 ± 0.118
1.602MetGly: 1.602 ± 0.171
0.601MetHis: 0.601 ± 0.102
1.559MetIle: 1.559 ± 0.13
1.53MetLys: 1.53 ± 0.151
2.617MetLeu: 2.617 ± 0.184
0.801MetMet: 0.801 ± 0.106
1.445MetAsn: 1.445 ± 0.122
1.402MetPro: 1.402 ± 0.146
0.858MetGln: 0.858 ± 0.107
1.23MetArg: 1.23 ± 0.125
2.517MetSer: 2.517 ± 0.196
1.53MetThr: 1.53 ± 0.134
2.188MetVal: 2.188 ± 0.19
0.286MetTrp: 0.286 ± 0.061
1.23MetTyr: 1.23 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.533AsnAla: 3.533 ± 0.249
0.501AsnCys: 0.501 ± 0.082
3.175AsnAsp: 3.175 ± 0.229
3.504AsnGlu: 3.504 ± 0.209
2.045AsnPhe: 2.045 ± 0.158
3.633AsnGly: 3.633 ± 0.264
0.973AsnHis: 0.973 ± 0.112
2.989AsnIle: 2.989 ± 0.181
3.104AsnLys: 3.104 ± 0.202
4.405AsnLeu: 4.405 ± 0.254
1.459AsnMet: 1.459 ± 0.153
2.374AsnAsn: 2.374 ± 0.228
2.603AsnPro: 2.603 ± 0.185
1.316AsnGln: 1.316 ± 0.122
3.204AsnArg: 3.204 ± 0.24
2.403AsnSer: 2.403 ± 0.203
2.846AsnThr: 2.846 ± 0.214
3.59AsnVal: 3.59 ± 0.248
0.715AsnTrp: 0.715 ± 0.099
2.489AsnTyr: 2.489 ± 0.205
0.0AsnXaa: 0.0 ± 0.0
Pro
2.503ProAla: 2.503 ± 0.21
0.3ProCys: 0.3 ± 0.074
3.047ProAsp: 3.047 ± 0.188
3.59ProGlu: 3.59 ± 0.274
1.473ProPhe: 1.473 ± 0.144
1.473ProGly: 1.473 ± 0.157
0.772ProHis: 0.772 ± 0.104
2.703ProIle: 2.703 ± 0.207
2.36ProLys: 2.36 ± 0.193
2.818ProLeu: 2.818 ± 0.208
1.087ProMet: 1.087 ± 0.122
1.888ProAsn: 1.888 ± 0.186
1.001ProPro: 1.001 ± 0.127
1.23ProGln: 1.23 ± 0.144
1.716ProArg: 1.716 ± 0.159
2.331ProSer: 2.331 ± 0.182
2.575ProThr: 2.575 ± 0.188
3.089ProVal: 3.089 ± 0.216
0.486ProTrp: 0.486 ± 0.082
1.831ProTyr: 1.831 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
2.446GlnAla: 2.446 ± 0.213
0.386GlnCys: 0.386 ± 0.071
1.702GlnAsp: 1.702 ± 0.15
2.131GlnGlu: 2.131 ± 0.191
1.473GlnPhe: 1.473 ± 0.152
1.516GlnGly: 1.516 ± 0.139
0.901GlnHis: 0.901 ± 0.103
1.745GlnIle: 1.745 ± 0.151
1.316GlnLys: 1.316 ± 0.138
3.261GlnLeu: 3.261 ± 0.22
1.101GlnMet: 1.101 ± 0.134
1.173GlnAsn: 1.173 ± 0.132
1.001GlnPro: 1.001 ± 0.102
1.173GlnGln: 1.173 ± 0.185
2.145GlnArg: 2.145 ± 0.182
2.045GlnSer: 2.045 ± 0.165
1.988GlnThr: 1.988 ± 0.18
2.26GlnVal: 2.26 ± 0.184
0.429GlnTrp: 0.429 ± 0.079
1.645GlnTyr: 1.645 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
3.747ArgAla: 3.747 ± 0.248
0.386ArgCys: 0.386 ± 0.088
4.348ArgAsp: 4.348 ± 0.345
3.948ArgGlu: 3.948 ± 0.253
2.06ArgPhe: 2.06 ± 0.191
3.304ArgGly: 3.304 ± 0.222
1.473ArgHis: 1.473 ± 0.154
3.848ArgIle: 3.848 ± 0.257
3.376ArgLys: 3.376 ± 0.189
4.191ArgLeu: 4.191 ± 0.277
1.774ArgMet: 1.774 ± 0.169
3.333ArgAsn: 3.333 ± 0.202
1.931ArgPro: 1.931 ± 0.155
1.902ArgGln: 1.902 ± 0.189
3.848ArgArg: 3.848 ± 0.35
3.218ArgSer: 3.218 ± 0.232
2.818ArgThr: 2.818 ± 0.202
3.747ArgVal: 3.747 ± 0.252
0.787ArgTrp: 0.787 ± 0.1
2.274ArgTyr: 2.274 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
3.461SerAla: 3.461 ± 0.255
0.515SerCys: 0.515 ± 0.103
4.391SerAsp: 4.391 ± 0.29
4.091SerGlu: 4.091 ± 0.241
2.446SerPhe: 2.446 ± 0.187
4.219SerGly: 4.219 ± 0.281
1.416SerHis: 1.416 ± 0.146
3.59SerIle: 3.59 ± 0.222
3.533SerLys: 3.533 ± 0.26
4.863SerLeu: 4.863 ± 0.231
1.831SerMet: 1.831 ± 0.217
2.889SerAsn: 2.889 ± 0.228
2.088SerPro: 2.088 ± 0.18
1.888SerGln: 1.888 ± 0.173
3.662SerArg: 3.662 ± 0.242
3.49SerSer: 3.49 ± 0.265
3.175SerThr: 3.175 ± 0.225
4.448SerVal: 4.448 ± 0.236
0.658SerTrp: 0.658 ± 0.102
2.088SerTyr: 2.088 ± 0.159
0.0SerXaa: 0.0 ± 0.0
Thr
4.52ThrAla: 4.52 ± 0.284
0.544ThrCys: 0.544 ± 0.091
3.762ThrAsp: 3.762 ± 0.227
3.461ThrGlu: 3.461 ± 0.21
2.646ThrPhe: 2.646 ± 0.205
3.404ThrGly: 3.404 ± 0.223
1.33ThrHis: 1.33 ± 0.139
4.305ThrIle: 4.305 ± 0.227
3.647ThrLys: 3.647 ± 0.259
5.578ThrLeu: 5.578 ± 0.323
1.659ThrMet: 1.659 ± 0.164
2.789ThrAsn: 2.789 ± 0.206
2.718ThrPro: 2.718 ± 0.184
1.859ThrGln: 1.859 ± 0.167
3.047ThrArg: 3.047 ± 0.198
3.075ThrSer: 3.075 ± 0.214
3.533ThrThr: 3.533 ± 0.233
4.663ThrVal: 4.663 ± 0.298
0.658ThrTrp: 0.658 ± 0.111
2.174ThrTyr: 2.174 ± 0.183
0.0ThrXaa: 0.0 ± 0.0
Val
4.906ValAla: 4.906 ± 0.307
0.787ValCys: 0.787 ± 0.096
5.492ValAsp: 5.492 ± 0.248
5.621ValGlu: 5.621 ± 0.279
2.331ValPhe: 2.331 ± 0.195
3.848ValGly: 3.848 ± 0.258
1.402ValHis: 1.402 ± 0.165
4.262ValIle: 4.262 ± 0.231
4.663ValLys: 4.663 ± 0.272
5.507ValLeu: 5.507 ± 0.339
2.045ValMet: 2.045 ± 0.17
3.662ValAsn: 3.662 ± 0.275
2.818ValPro: 2.818 ± 0.222
1.945ValGln: 1.945 ± 0.162
4.091ValArg: 4.091 ± 0.245
4.134ValSer: 4.134 ± 0.213
4.177ValThr: 4.177 ± 0.248
4.763ValVal: 4.763 ± 0.256
0.729ValTrp: 0.729 ± 0.091
3.018ValTyr: 3.018 ± 0.231
0.0ValXaa: 0.0 ± 0.0
Trp
0.629TrpAla: 0.629 ± 0.097
0.043TrpCys: 0.043 ± 0.028
0.901TrpAsp: 0.901 ± 0.106
1.001TrpGlu: 1.001 ± 0.109
0.572TrpPhe: 0.572 ± 0.087
0.486TrpGly: 0.486 ± 0.101
0.372TrpHis: 0.372 ± 0.072
0.644TrpIle: 0.644 ± 0.089
0.772TrpLys: 0.772 ± 0.108
1.244TrpLeu: 1.244 ± 0.137
0.415TrpMet: 0.415 ± 0.066
0.687TrpAsn: 0.687 ± 0.1
0.386TrpPro: 0.386 ± 0.077
0.372TrpGln: 0.372 ± 0.074
0.644TrpArg: 0.644 ± 0.091
0.744TrpSer: 0.744 ± 0.114
0.758TrpThr: 0.758 ± 0.107
0.729TrpVal: 0.729 ± 0.095
0.114TrpTrp: 0.114 ± 0.039
0.572TrpTyr: 0.572 ± 0.081
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.432TyrAla: 2.432 ± 0.173
0.529TyrCys: 0.529 ± 0.093
2.889TyrAsp: 2.889 ± 0.217
2.56TyrGlu: 2.56 ± 0.209
1.645TyrPhe: 1.645 ± 0.191
2.689TyrGly: 2.689 ± 0.21
1.259TyrHis: 1.259 ± 0.138
2.288TyrIle: 2.288 ± 0.193
1.945TyrLys: 1.945 ± 0.152
3.561TyrLeu: 3.561 ± 0.235
1.03TyrMet: 1.03 ± 0.127
2.131TyrAsn: 2.131 ± 0.168
1.645TyrPro: 1.645 ± 0.174
1.344TyrGln: 1.344 ± 0.14
2.446TyrArg: 2.446 ± 0.172
2.331TyrSer: 2.331 ± 0.204
2.36TyrThr: 2.36 ± 0.161
3.247TyrVal: 3.247 ± 0.225
0.515TyrTrp: 0.515 ± 0.082
1.502TyrTyr: 1.502 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 241 proteins (69916 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski