Candidimonas sp. SYP-B2681
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3632 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432DWA2|A0A432DWA2_9BURK Disulfide bond formation protein B OS=Candidimonas sp. SYP-B2681 OX=2497686 GN=EKL30_02860 PE=4 SV=1
MM1 pKa = 7.32 NVHH4 pKa = 5.14 SHH6 pKa = 5.75 IVEE9 pKa = 4.15 TLQQGVVLDD18 pKa = 3.88 VSIEE22 pKa = 4.03 DD23 pKa = 3.49 LHH25 pKa = 7.01 FRR27 pKa = 11.84 AYY29 pKa = 10.38 VVLSEE34 pKa = 4.18 PDD36 pKa = 3.21 INLVADD42 pKa = 4.64 FVPPEE47 pKa = 4.0 QFEE50 pKa = 4.16 QDD52 pKa = 3.22 GDD54 pKa = 3.98 VHH56 pKa = 6.46 VAAISHH62 pKa = 6.85 PDD64 pKa = 3.3 EE65 pKa = 4.0 VHH67 pKa = 6.46 EE68 pKa = 4.36 QIQDD72 pKa = 2.87 ITFNMNPGDD81 pKa = 3.74 AAVFMCSDD89 pKa = 2.75 QRR91 pKa = 11.84 TYY93 pKa = 11.66 LDD95 pKa = 3.49 ALEE98 pKa = 5.17 EE99 pKa = 4.37 LGQSNNAIQDD109 pKa = 3.38
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.668
ProMoST 3.973
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A432DP70|A0A432DP70_9BURK CoA transferase OS=Candidimonas sp. SYP-B2681 OX=2497686 GN=EKL30_07455 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.39 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3632
0
3632
1176441
24
2840
323.9
35.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.213 ± 0.044
0.926 ± 0.013
5.271 ± 0.03
5.254 ± 0.038
3.699 ± 0.026
7.954 ± 0.037
2.248 ± 0.021
5.545 ± 0.027
3.683 ± 0.035
10.59 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.018
3.128 ± 0.022
4.96 ± 0.024
3.94 ± 0.028
6.143 ± 0.034
6.105 ± 0.025
5.323 ± 0.023
7.52 ± 0.035
1.387 ± 0.018
2.507 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here