Pseudofulvimonas gallinarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Pseudofulvimonas

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3UU02|A0A4V3UU02_9GAMM Uncharacterized protein DUF4340 OS=Pseudofulvimonas gallinarii OX=634155 GN=EDC25_11847 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84IVAAGMLALASAYY15 pKa = 10.24AGAAEE20 pKa = 4.24VTVKK24 pKa = 10.69NDD26 pKa = 2.75SLTDD30 pKa = 3.95FGTAAIVWGFVEE42 pKa = 4.54GEE44 pKa = 4.3KK45 pKa = 10.34AASWLTSPCNGTLRR59 pKa = 11.84AVQIFWRR66 pKa = 11.84SPSGTSGQSIHH77 pKa = 7.37DD78 pKa = 4.2SIEE81 pKa = 3.66IFRR84 pKa = 11.84SGTFPTPGALVEE96 pKa = 4.7TIAGPVLADD105 pKa = 3.42GALNEE110 pKa = 3.94WRR112 pKa = 11.84YY113 pKa = 10.38VDD115 pKa = 3.94EE116 pKa = 4.84NNLVPLAVDD125 pKa = 3.36VAEE128 pKa = 4.5NEE130 pKa = 4.28TLVVSFTFDD139 pKa = 3.27HH140 pKa = 6.69EE141 pKa = 4.36PEE143 pKa = 4.09QLVAPSVVRR152 pKa = 11.84DD153 pKa = 3.61SDD155 pKa = 4.07GVQPNRR161 pKa = 11.84NGLYY165 pKa = 11.05AMLAPGTYY173 pKa = 8.2MWFNSASLGVAGDD186 pKa = 3.46WVIRR190 pKa = 11.84AVIDD194 pKa = 4.0CPVVDD199 pKa = 5.11PEE201 pKa = 5.14ADD203 pKa = 3.45VAVDD207 pKa = 3.44MTADD211 pKa = 3.45AEE213 pKa = 5.08GYY215 pKa = 8.67TPGQPLTYY223 pKa = 9.79TVVVDD228 pKa = 3.83NAGPAAATSTTIVDD242 pKa = 3.53VFPAAFTSPAWTCTPSGGATCPASGSGNITHH273 pKa = 6.99VISLPAGGSATYY285 pKa = 9.83EE286 pKa = 3.66ITGTVSASASGTLSNSFSAVVGGIIDD312 pKa = 4.45PEE314 pKa = 4.27PSNNTVTLEE323 pKa = 4.21LEE325 pKa = 4.17EE326 pKa = 5.38LLDD329 pKa = 5.2DD330 pKa = 4.41DD331 pKa = 4.83TVFANGFEE339 pKa = 4.49DD340 pKa = 4.07GG341 pKa = 4.11

Molecular weight: 35.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3L111|A0A4R3L111_9GAMM Uncharacterized protein (Fragment) OS=Pseudofulvimonas gallinarii OX=634155 GN=EDC25_13127 PE=4 SV=1
MM1 pKa = 7.21ATTRR5 pKa = 11.84KK6 pKa = 9.34AAKK9 pKa = 9.91KK10 pKa = 8.6PAARR14 pKa = 11.84KK15 pKa = 8.09ATAKK19 pKa = 10.56KK20 pKa = 8.94ATKK23 pKa = 9.97KK24 pKa = 9.32VAKK27 pKa = 10.04KK28 pKa = 9.2ATAKK32 pKa = 10.59KK33 pKa = 8.84ATKK36 pKa = 9.97KK37 pKa = 9.32VAKK40 pKa = 10.04KK41 pKa = 9.2ATAKK45 pKa = 10.36KK46 pKa = 9.0ATRR49 pKa = 11.84KK50 pKa = 8.86VAKK53 pKa = 8.88KK54 pKa = 5.97TTARR58 pKa = 11.84KK59 pKa = 8.26ATAKK63 pKa = 10.48KK64 pKa = 9.1ATAKK68 pKa = 10.58KK69 pKa = 8.84ATKK72 pKa = 9.97KK73 pKa = 9.32VAKK76 pKa = 10.04KK77 pKa = 9.26ATAKK81 pKa = 10.33KK82 pKa = 9.47AAKK85 pKa = 9.74KK86 pKa = 8.97ATRR89 pKa = 11.84KK90 pKa = 8.87VAKK93 pKa = 8.88KK94 pKa = 5.97TTARR98 pKa = 11.84KK99 pKa = 8.26ATAKK103 pKa = 10.32KK104 pKa = 9.09ATRR107 pKa = 11.84KK108 pKa = 8.51VAKK111 pKa = 10.07KK112 pKa = 9.11ATAKK116 pKa = 10.36KK117 pKa = 9.0ATRR120 pKa = 11.84KK121 pKa = 8.51VAKK124 pKa = 10.07KK125 pKa = 9.11ATAKK129 pKa = 10.55KK130 pKa = 9.03ATAKK134 pKa = 10.53KK135 pKa = 9.12ATAKK139 pKa = 10.19KK140 pKa = 9.94AARR143 pKa = 11.84KK144 pKa = 8.28PAAKK148 pKa = 9.92KK149 pKa = 9.11AAKK152 pKa = 10.1RR153 pKa = 11.84PAKK156 pKa = 8.68KK157 pKa = 8.85TKK159 pKa = 9.46PAVKK163 pKa = 10.03KK164 pKa = 10.12AVKK167 pKa = 9.85PVTTPIAPVLSSTVQQ182 pKa = 2.84

Molecular weight: 19.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2887

0

2887

1021112

26

4943

353.7

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.879 ± 0.064

0.905 ± 0.016

6.123 ± 0.034

5.316 ± 0.046

3.346 ± 0.025

8.992 ± 0.048

2.309 ± 0.022

4.183 ± 0.033

1.987 ± 0.033

10.834 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.02

2.52 ± 0.034

5.432 ± 0.034

3.517 ± 0.024

8.225 ± 0.06

5.319 ± 0.033

4.877 ± 0.044

7.508 ± 0.044

1.538 ± 0.02

2.156 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski