Streptococcus satellite phage Javan537
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUQ0|A0A4D5ZUQ0_9VIRU LmaC OS=Streptococcus satellite phage Javan537 OX=2558731 GN=JavanS537_0006 PE=4 SV=1
MM1 pKa = 7.1 TFKK4 pKa = 10.76 FFSDD8 pKa = 3.2 NAKK11 pKa = 10.59 SFTFKK16 pKa = 11.15 YY17 pKa = 10.52 DD18 pKa = 3.33 FEE20 pKa = 4.84 TMDD23 pKa = 3.83 HH24 pKa = 6.67 ANVSSTAILGYY35 pKa = 9.44 MVGTYY40 pKa = 7.14 EE41 pKa = 4.12 QQPTEE46 pKa = 3.74 ITIGGNEE53 pKa = 4.22 ANNTFTMVVKK63 pKa = 10.59 YY64 pKa = 10.86 VEE66 pKa = 5.52 DD67 pKa = 3.78 EE68 pKa = 4.43 DD69 pKa = 3.79 LTKK72 pKa = 10.67 VFNRR76 pKa = 11.84 ICKK79 pKa = 10.14 SFEE82 pKa = 4.28 SYY84 pKa = 11.02 SKK86 pKa = 11.06 GYY88 pKa = 10.45 DD89 pKa = 3.21 EE90 pKa = 4.88 EE91 pKa = 4.44 AA92 pKa = 4.07
Molecular weight: 10.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.408
IPC2_protein 4.533
IPC_protein 4.393
Toseland 4.228
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.202
Rodwell 4.228
Grimsley 4.151
Solomon 4.329
Lehninger 4.279
Nozaki 4.444
DTASelect 4.584
Thurlkill 4.24
EMBOSS 4.228
Sillero 4.495
Patrickios 3.35
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.435
Protein with the highest isoelectric point:
>tr|A0A4D5ZS25|A0A4D5ZS25_9VIRU Paratox OS=Streptococcus satellite phage Javan537 OX=2558731 GN=JavanS537_0001 PE=4 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 KK3 pKa = 8.09 TIPNTHH9 pKa = 5.77 KK10 pKa = 10.61 RR11 pKa = 11.84 ILASIPVGADD21 pKa = 2.86 SRR23 pKa = 11.84 SVTGKK28 pKa = 10.05 EE29 pKa = 3.42 LAATHH34 pKa = 6.45 RR35 pKa = 11.84 MDD37 pKa = 4.14 RR38 pKa = 11.84 RR39 pKa = 11.84 TIQAIIRR46 pKa = 11.84 RR47 pKa = 11.84 LIVEE51 pKa = 4.33 YY52 pKa = 10.31 GVPICASRR60 pKa = 11.84 DD61 pKa = 3.3 INGGYY66 pKa = 9.82 FIPANDD72 pKa = 3.68 TEE74 pKa = 4.29 RR75 pKa = 11.84 LEE77 pKa = 4.69 GIRR80 pKa = 11.84 ALKK83 pKa = 10.26 SQLTEE88 pKa = 3.64 EE89 pKa = 4.52 EE90 pKa = 4.32 KK91 pKa = 10.66 RR92 pKa = 11.84 VSALLEE98 pKa = 4.47 ADD100 pKa = 3.08 LTEE103 pKa = 4.21 YY104 pKa = 11.14 KK105 pKa = 10.5 EE106 pKa = 4.06 LLRR109 pKa = 11.84 GG110 pKa = 3.53
Molecular weight: 12.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.075
IPC2_protein 8.814
IPC_protein 8.931
Toseland 9.56
ProMoST 9.297
Dawson 9.794
Bjellqvist 9.516
Wikipedia 9.97
Rodwell 10.028
Grimsley 9.867
Solomon 9.897
Lehninger 9.867
Nozaki 9.619
DTASelect 9.487
Thurlkill 9.633
EMBOSS 9.97
Sillero 9.736
Patrickios 7.658
IPC_peptide 9.882
IPC2_peptide 8.185
IPC2.peptide.svr19 7.852
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2637
48
388
146.5
16.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.257 ± 0.645
0.796 ± 0.104
5.271 ± 0.324
8.267 ± 0.891
4.285 ± 0.568
4.323 ± 0.459
1.82 ± 0.348
6.864 ± 0.452
9.215 ± 0.696
9.48 ± 0.448
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.236
6.598 ± 0.343
3.072 ± 0.34
3.906 ± 0.369
4.854 ± 0.558
5.347 ± 0.325
7.243 ± 0.591
4.551 ± 0.365
0.683 ± 0.119
5.006 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here