Streptococcus satellite phage Javan415
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPH6|A0A4D5ZPH6_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan415 OX=2558690 GN=JavanS415_0012 PE=4 SV=1
MM1 pKa = 7.88 VNITDD6 pKa = 3.82 VKK8 pKa = 10.81 QILQFAIDD16 pKa = 3.74 AEE18 pKa = 4.38 IKK20 pKa = 10.71 VFLDD24 pKa = 3.67 GGWGVDD30 pKa = 3.37 ALLGYY35 pKa = 8.24 QSRR38 pKa = 11.84 AHH40 pKa = 6.36 NDD42 pKa = 2.16 IDD44 pKa = 4.33 IFVEE48 pKa = 4.03 KK49 pKa = 10.57 NDD51 pKa = 3.52 YY52 pKa = 11.06 QNFIEE57 pKa = 4.35 IMKK60 pKa = 10.8 ANGFYY65 pKa = 9.81 EE66 pKa = 3.97 IKK68 pKa = 9.96 MEE70 pKa = 4.09 YY71 pKa = 7.09 TTLNHH76 pKa = 5.95 TVWEE80 pKa = 4.16 DD81 pKa = 3.21 LKK83 pKa = 11.51 NRR85 pKa = 11.84 IIDD88 pKa = 3.56 LHH90 pKa = 7.38 CFEE93 pKa = 4.89 YY94 pKa = 10.57 TDD96 pKa = 3.95 EE97 pKa = 5.66 GEE99 pKa = 3.93 ILYY102 pKa = 10.75 DD103 pKa = 3.82 GDD105 pKa = 4.27 CFPVEE110 pKa = 4.2 TFSGKK115 pKa = 10.15 GRR117 pKa = 11.84 IEE119 pKa = 4.42 EE120 pKa = 4.37 IEE122 pKa = 4.28 VSCIEE127 pKa = 4.83 PYY129 pKa = 9.55 SQVMFHH135 pKa = 6.87 LGYY138 pKa = 10.91 EE139 pKa = 4.04 FDD141 pKa = 4.65 EE142 pKa = 4.84 NDD144 pKa = 3.22 AHH146 pKa = 7.24 DD147 pKa = 4.19 VKK149 pKa = 11.0 LLCEE153 pKa = 4.15 TFHH156 pKa = 8.87 IEE158 pKa = 4.46 IPNEE162 pKa = 3.74 YY163 pKa = 10.09 RR164 pKa = 3.46
Molecular weight: 19.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.991
IPC2_protein 4.279
IPC_protein 4.228
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.457
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.342
Patrickios 1.964
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>tr|A0A4D5ZSM1|A0A4D5ZSM1_9VIRU Integrase OS=Streptococcus satellite phage Javan415 OX=2558690 GN=JavanS415_0013 PE=3 SV=1
MM1 pKa = 7.39 FLHH4 pKa = 6.8 FPIILDD10 pKa = 4.48 GIIEE14 pKa = 4.21 ADD16 pKa = 3.38 EE17 pKa = 4.25 TFFAISYY24 pKa = 9.35 KK25 pKa = 10.68 GNHH28 pKa = 6.13 SKK30 pKa = 10.92 SKK32 pKa = 8.95 TFAMPRR38 pKa = 11.84 KK39 pKa = 7.79 AHH41 pKa = 6.1 KK42 pKa = 9.66 RR43 pKa = 11.84 GHH45 pKa = 4.94 STHH48 pKa = 6.35 IRR50 pKa = 11.84 GLSQEE55 pKa = 4.4 KK56 pKa = 10.23 VCVPCAVNRR65 pKa = 11.84 NGLSISKK72 pKa = 8.26 ITNTGRR78 pKa = 11.84 VSTRR82 pKa = 11.84 DD83 pKa = 3.09 LHH85 pKa = 7.18 HH86 pKa = 7.07 IYY88 pKa = 10.41 DD89 pKa = 3.91 GRR91 pKa = 11.84 IMTNSTLVTDD101 pKa = 4.3 KK102 pKa = 10.3 MNSYY106 pKa = 10.45 VRR108 pKa = 11.84 FTNANGIDD116 pKa = 4.04 LVQLKK121 pKa = 8.99 TGKK124 pKa = 9.91 AKK126 pKa = 10.54 KK127 pKa = 10.1 GIYY130 pKa = 9.6 NIQHH134 pKa = 5.91 INSYY138 pKa = 10.3 HH139 pKa = 5.54 SQLKK143 pKa = 9.97 RR144 pKa = 11.84 FMRR147 pKa = 11.84 GFNGVSTKK155 pKa = 10.69 YY156 pKa = 10.69 LNNYY160 pKa = 7.83 LVWNNLVNYY169 pKa = 9.92 AKK171 pKa = 10.47 EE172 pKa = 3.76 SDD174 pKa = 3.53 MEE176 pKa = 4.07 KK177 pKa = 10.79 RR178 pKa = 11.84 NIFLTFVLATLKK190 pKa = 9.45 TAKK193 pKa = 10.3 CRR195 pKa = 11.84 DD196 pKa = 3.51 LSNRR200 pKa = 11.84 PAVPLVAA207 pKa = 5.64
Molecular weight: 23.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.202
IPC2_protein 9.531
IPC_protein 9.575
Toseland 10.277
ProMoST 9.882
Dawson 10.423
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.965
Grimsley 10.482
Solomon 10.452
Lehninger 10.423
Nozaki 10.262
DTASelect 10.058
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.335
Patrickios 10.643
IPC_peptide 10.452
IPC2_peptide 8.756
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2485
61
477
191.2
22.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.473 ± 0.506
0.644 ± 0.191
5.755 ± 0.785
8.571 ± 0.85
4.185 ± 0.33
4.99 ± 0.356
1.73 ± 0.423
7.243 ± 0.596
9.658 ± 0.676
8.813 ± 0.608
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.321
6.278 ± 0.453
3.018 ± 0.407
2.897 ± 0.414
4.99 ± 0.419
4.829 ± 0.566
6.318 ± 0.468
5.835 ± 0.348
0.926 ± 0.143
5.352 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here