Arabis nemorensis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A565BPQ4|A0A565BPQ4_9BRAS Uncharacterized protein OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS14000 PE=3 SV=1
MM1 pKa = 8.17DD2 pKa = 6.09PNQNTCPIDD11 pKa = 3.55IDD13 pKa = 5.28DD14 pKa = 5.16FLDD17 pKa = 4.22DD18 pKa = 5.8LLDD21 pKa = 3.94FDD23 pKa = 6.01FGFDD27 pKa = 3.82DD28 pKa = 4.21EE29 pKa = 4.87VGQGIHH35 pKa = 5.61NQAEE39 pKa = 4.5VVHH42 pKa = 6.48NGVHH46 pKa = 5.09NQADD50 pKa = 3.94VVHH53 pKa = 7.28IEE55 pKa = 3.89ASQVIQNVCKK65 pKa = 10.6SYY67 pKa = 11.79ANN69 pKa = 3.57

Molecular weight:
7.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A565CBM6|A0A565CBM6_9BRAS DDE Tnp4 domain-containing protein OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS21554 PE=3 SV=1
MM1 pKa = 7.74ASLSTSVVGSASSRR15 pKa = 11.84LWLPSSNDD23 pKa = 3.61KK24 pKa = 10.87ISVPSASLSLRR35 pKa = 11.84SGSRR39 pKa = 11.84RR40 pKa = 11.84SPFSLNSSPSVSAXXXXXXXXXXXXXXXXASTFSGLSIAFDD81 pKa = 3.85LRR83 pKa = 11.84SGTSGLNGQRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84GLVVRR100 pKa = 11.84AGKK103 pKa = 9.83AALCQTKK110 pKa = 10.09RR111 pKa = 11.84SRR113 pKa = 11.84SRR115 pKa = 11.84KK116 pKa = 9.07SLARR120 pKa = 11.84THH122 pKa = 6.34GFRR125 pKa = 11.84LRR127 pKa = 11.84MRR129 pKa = 11.84TTSGRR134 pKa = 11.84ATIKK138 pKa = 10.11RR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84AKK143 pKa = 10.46GRR145 pKa = 11.84WNLCPKK151 pKa = 10.26SNPSSGKK158 pKa = 9.72RR159 pKa = 11.84AA160 pKa = 3.32

Molecular weight:
15.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28762

0

28762

10701967

49

5082

372.1

41.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.399 ± 0.012

1.785 ± 0.007

5.326 ± 0.01

6.717 ± 0.019

4.202 ± 0.01

6.426 ± 0.014

2.228 ± 0.006

5.245 ± 0.01

6.289 ± 0.012

9.46 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.005

4.327 ± 0.01

4.776 ± 0.013

3.451 ± 0.009

5.493 ± 0.011

8.974 ± 0.015

5.059 ± 0.011

6.668 ± 0.01

1.257 ± 0.005

2.756 ± 0.008

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski