Arabis nemorensis
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A565BPQ4|A0A565BPQ4_9BRAS Uncharacterized protein OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS14000 PE=3 SV=1
MM1 pKa = 8.17 DD2 pKa = 6.09 PNQNTCPIDD11 pKa = 3.55 IDD13 pKa = 5.28 DD14 pKa = 5.16 FLDD17 pKa = 4.22 DD18 pKa = 5.8 LLDD21 pKa = 3.94 FDD23 pKa = 6.01 FGFDD27 pKa = 3.82 DD28 pKa = 4.21 EE29 pKa = 4.87 VGQGIHH35 pKa = 5.61 NQAEE39 pKa = 4.5 VVHH42 pKa = 6.48 NGVHH46 pKa = 5.09 NQADD50 pKa = 3.94 VVHH53 pKa = 7.28 IEE55 pKa = 3.89 ASQVIQNVCKK65 pKa = 10.6 SYY67 pKa = 11.79 ANN69 pKa = 3.57
Molecular weight: 7.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.503
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A565CBM6|A0A565CBM6_9BRAS DDE Tnp4 domain-containing protein OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS21554 PE=3 SV=1
MM1 pKa = 7.74 ASLSTSVVGSASSRR15 pKa = 11.84 LWLPSSNDD23 pKa = 3.61 KK24 pKa = 10.87 ISVPSASLSLRR35 pKa = 11.84 SGSRR39 pKa = 11.84 RR40 pKa = 11.84 SPFSLNSSPSVSAXXXXXXXXXXXXXXXXASTFSGLSIAFDD81 pKa = 3.85 LRR83 pKa = 11.84 SGTSGLNGQRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 GLVVRR100 pKa = 11.84 AGKK103 pKa = 9.83 AALCQTKK110 pKa = 10.09 RR111 pKa = 11.84 SRR113 pKa = 11.84 SRR115 pKa = 11.84 KK116 pKa = 9.07 SLARR120 pKa = 11.84 THH122 pKa = 6.34 GFRR125 pKa = 11.84 LRR127 pKa = 11.84 MRR129 pKa = 11.84 TTSGRR134 pKa = 11.84 ATIKK138 pKa = 10.11 RR139 pKa = 11.84 RR140 pKa = 11.84 RR141 pKa = 11.84 AKK143 pKa = 10.46 GRR145 pKa = 11.84 WNLCPKK151 pKa = 10.26 SNPSSGKK158 pKa = 9.72 RR159 pKa = 11.84 AA160 pKa = 3.32
Molecular weight: 15.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.544
ProMoST 13.027
Dawson 12.544
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.193
Grimsley 12.574
Solomon 13.027
Lehninger 12.939
Nozaki 12.544
DTASelect 12.53
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.915
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28762
0
28762
10701967
49
5082
372.1
41.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.399 ± 0.012
1.785 ± 0.007
5.326 ± 0.01
6.717 ± 0.019
4.202 ± 0.01
6.426 ± 0.014
2.228 ± 0.006
5.245 ± 0.01
6.289 ± 0.012
9.46 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.466 ± 0.005
4.327 ± 0.01
4.776 ± 0.013
3.451 ± 0.009
5.493 ± 0.011
8.974 ± 0.015
5.059 ± 0.011
6.668 ± 0.01
1.257 ± 0.005
2.756 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here