Sphingorhabdus sp. JK6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Parasphingorhabdus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2DHW0|A0A6H2DHW0_9SPHN MarR family transcriptional regulator OS=Sphingorhabdus sp. JK6 OX=2725558 GN=HF685_02210 PE=4 SV=1
MM1 pKa = 7.11TSKK4 pKa = 11.02LKK6 pKa = 11.05LLVATSAFSVAMLGATPAFAVGTDD30 pKa = 3.08AGVPITNSVNITYY43 pKa = 10.44NVGGAAQTPPPVASDD58 pKa = 3.37TFVVDD63 pKa = 3.93RR64 pKa = 11.84KK65 pKa = 11.07VNLLVQRR72 pKa = 11.84FDD74 pKa = 5.57AIDD77 pKa = 3.56TPVAPNQTGAAVAYY91 pKa = 7.82TVTNLTNDD99 pKa = 4.07TIDD102 pKa = 3.89VILSAEE108 pKa = 4.06QTGGDD113 pKa = 4.23DD114 pKa = 4.09FDD116 pKa = 3.9STGAFTYY123 pKa = 9.33YY124 pKa = 10.64TDD126 pKa = 3.52VNQDD130 pKa = 3.17GLLDD134 pKa = 4.27GGDD137 pKa = 3.91TILASGVIDD146 pKa = 4.44NLAEE150 pKa = 4.06DD151 pKa = 5.24AIAYY155 pKa = 9.55ILVTSNIPTNNSSASPLANGEE176 pKa = 4.18TGDD179 pKa = 4.58VILIGQAANAATGTAFSDD197 pKa = 3.99DD198 pKa = 3.83AGNVNAAGTVQNVFADD214 pKa = 3.52ADD216 pKa = 4.38GPGSTDD222 pKa = 3.14VAGATALLGDD232 pKa = 4.46GRR234 pKa = 11.84HH235 pKa = 5.64SAIDD239 pKa = 3.6TYY241 pKa = 11.66VVDD244 pKa = 3.7TATLSVAKK252 pKa = 9.73TSVILWDD259 pKa = 4.96PINGSTNPKK268 pKa = 9.4AIPGAIVQYY277 pKa = 10.51CIAVSNASTTVAATNVAISDD297 pKa = 4.28SIAALALTYY306 pKa = 10.77YY307 pKa = 8.62GTTVASGPATIPTATAAPTSATACDD332 pKa = 4.15GTGSNPDD339 pKa = 3.32ASALSHH345 pKa = 6.18SSGVISGNLGNVAASSTEE363 pKa = 3.82TLIFRR368 pKa = 11.84ATVNN372 pKa = 3.19

Molecular weight: 36.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2DMH9|A0A6H2DMH9_9SPHN Glycerophosphodiester phosphodiesterase OS=Sphingorhabdus sp. JK6 OX=2725558 GN=HF685_08630 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.57GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.68ATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3022

0

3022

940177

30

2434

311.1

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.073 ± 0.047

0.828 ± 0.015

6.163 ± 0.036

5.99 ± 0.048

3.926 ± 0.034

8.509 ± 0.062

1.904 ± 0.026

6.136 ± 0.038

4.536 ± 0.041

9.357 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.023

3.504 ± 0.035

4.706 ± 0.028

3.354 ± 0.024

5.691 ± 0.038

5.952 ± 0.038

5.225 ± 0.038

6.64 ± 0.037

1.355 ± 0.019

2.411 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski