Oenococcus phage vB_OeS_unk162
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6S4IMJ1|A0A6S4IMJ1_9VIRU Uncharacterized protein OS=Oenococcus phage vB_OeS_unk162 OX=2036701 GN=vBOeSunk162_22 PE=4 SV=1
MM1 pKa = 8.01 AKK3 pKa = 10.22 PKK5 pKa = 10.0 IDD7 pKa = 3.69 VMEE10 pKa = 4.4 SLQDD14 pKa = 4.05 SINANCDD21 pKa = 3.56 LQGMNLTIGFLTPNNPLTILDD42 pKa = 4.24 GSSSTVLEE50 pKa = 3.98 RR51 pKa = 11.84 FMDD54 pKa = 4.24 GGQIVSYY61 pKa = 9.07 PYY63 pKa = 10.29 QIAIRR68 pKa = 11.84 HH69 pKa = 5.4 TSQEE73 pKa = 4.01 VISQVLYY80 pKa = 10.67 EE81 pKa = 4.07 IYY83 pKa = 10.26 EE84 pKa = 4.06 YY85 pKa = 11.42 LNNDD89 pKa = 3.46 DD90 pKa = 5.84 FSIEE94 pKa = 4.17 SSNNTWQFEE103 pKa = 4.33 TASATKK109 pKa = 9.65 QQPSGKK115 pKa = 9.24 DD116 pKa = 2.99 QEE118 pKa = 4.32 NNYY121 pKa = 9.68 VFVLNLQITISFF133 pKa = 3.99
Molecular weight: 15.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 4.126
IPC_protein 4.024
Toseland 3.846
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.91
Rodwell 3.859
Grimsley 3.757
Solomon 3.986
Lehninger 3.948
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.04
Protein with the highest isoelectric point:
>tr|A0A6S4INR3|A0A6S4INR3_9VIRU Major capsid protein OS=Oenococcus phage vB_OeS_unk162 OX=2036701 GN=vBOeSunk162_08 PE=4 SV=1
MM1 pKa = 7.72 KK2 pKa = 10.42 NRR4 pKa = 11.84 NTYY7 pKa = 10.31 KK8 pKa = 10.28 ILTLISVIFPLDD20 pKa = 2.92 RR21 pKa = 11.84 LYY23 pKa = 10.35 IGRR26 pKa = 11.84 HH27 pKa = 4.05 CFIRR31 pKa = 11.84 CFSLNLLGIGWLQDD45 pKa = 2.99 LFYY48 pKa = 10.68 RR49 pKa = 11.84 DD50 pKa = 3.48 KK51 pKa = 11.4 TFDD54 pKa = 2.94 EE55 pKa = 4.34 AMARR59 pKa = 11.84 RR60 pKa = 11.84 GFNNTTIRR68 pKa = 11.84 NADD71 pKa = 3.1 GRR73 pKa = 4.1
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.428
IPC_protein 9.955
Toseland 10.014
ProMoST 10.101
Dawson 10.262
Bjellqvist 10.028
Wikipedia 10.482
Rodwell 10.423
Grimsley 10.35
Solomon 10.335
Lehninger 10.292
Nozaki 10.101
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.204
Patrickios 10.116
IPC_peptide 10.321
IPC2_peptide 9.194
IPC2.peptide.svr19 8.407
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
11484
49
2170
225.2
25.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.958 ± 0.538
0.348 ± 0.062
7.297 ± 0.36
5.286 ± 0.553
4.328 ± 0.32
6.217 ± 0.684
1.533 ± 0.186
7.497 ± 0.344
7.593 ± 1.053
7.297 ± 0.334
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.238 ± 0.254
6.54 ± 0.326
2.778 ± 0.213
4.31 ± 0.308
2.699 ± 0.449
8.159 ± 0.723
7.802 ± 0.914
5.825 ± 0.309
1.315 ± 0.162
3.979 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here