Bacteroides graminisolvens DSM 19988 = JCM 15093

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides graminisolvens

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A069D304|A0A069D304_9BACE Two-component system sensor histidine kinase/response regulator hybrid OS=Bacteroides graminisolvens DSM 19988 = JCM 15093 OX=1121097 GN=JCM15093_1895 PE=4 SV=1
MM1 pKa = 7.54IIAAAFVVLGTTSCGDD17 pKa = 3.74DD18 pKa = 3.59FLTASSTEE26 pKa = 3.87KK27 pKa = 10.76QEE29 pKa = 4.24AGGAATEE36 pKa = 4.21GAILSNLGSAYY47 pKa = 10.36QILLFDD53 pKa = 4.49SYY55 pKa = 12.19ANNNYY60 pKa = 10.12NSVPLMSDD68 pKa = 3.26LRR70 pKa = 11.84SDD72 pKa = 5.11DD73 pKa = 3.54IYY75 pKa = 11.44KK76 pKa = 10.87GGGDD80 pKa = 4.82AGDD83 pKa = 3.27QGQLYY88 pKa = 10.08KK89 pKa = 10.9LSQFTSSADD98 pKa = 3.68EE99 pKa = 3.92II100 pKa = 4.46

Molecular weight: 10.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A069CYQ1|A0A069CYQ1_9BACE Putative TonB-dependent receptor OS=Bacteroides graminisolvens DSM 19988 = JCM 15093 OX=1121097 GN=JCM15093_340 PE=4 SV=1
MM1 pKa = 8.02SVMKK5 pKa = 10.42KK6 pKa = 9.61QLVYY10 pKa = 11.23LLTGWLMCGMGLQARR25 pKa = 11.84AQVEE29 pKa = 3.97PLFTYY34 pKa = 10.23KK35 pKa = 10.32ISRR38 pKa = 11.84DD39 pKa = 3.53EE40 pKa = 4.15ACNRR44 pKa = 11.84WVEE47 pKa = 4.24SQMKK51 pKa = 9.64QMNLRR56 pKa = 11.84EE57 pKa = 4.57KK58 pKa = 10.22IGQLFIYY65 pKa = 8.77TIAPDD70 pKa = 3.34RR71 pKa = 11.84SKK73 pKa = 11.48RR74 pKa = 11.84NLALLKK80 pKa = 10.74NVVHH84 pKa = 5.63TQSGRR89 pKa = 11.84IAFLGRR95 pKa = 11.84KK96 pKa = 6.66TRR98 pKa = 3.53

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3344

0

3344

1078488

37

1749

322.5

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.149 ± 0.039

1.173 ± 0.016

5.409 ± 0.029

6.354 ± 0.041

4.592 ± 0.028

6.784 ± 0.039

1.846 ± 0.019

6.911 ± 0.046

6.867 ± 0.036

9.186 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.02

5.359 ± 0.039

3.615 ± 0.026

3.492 ± 0.024

4.322 ± 0.028

6.409 ± 0.038

5.566 ± 0.031

6.593 ± 0.032

1.269 ± 0.02

4.376 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski