Cloacibacterium normanense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Cloacibacterium

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5UCU9|A0A1E5UCU9_9FLAO Putative polysaccharide capsule synthesis protein CpsH OS=Cloacibacterium normanense OX=237258 GN=BHF72_0127 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 8.61TLNFKK8 pKa = 8.15TFKK11 pKa = 10.46TFLQLIFLVVGVLGLAQTYY30 pKa = 7.7PALEE34 pKa = 4.48FGNVTPSPNTNTSLSFDD51 pKa = 3.83FQKK54 pKa = 10.42DD55 pKa = 3.72TNNNTATNLVNYY67 pKa = 7.32TSPSVLTVSYY77 pKa = 10.8NVTATSFPNGLRR89 pKa = 11.84FGAGGAAPFYY99 pKa = 10.57TLMNAIGNAGSDD111 pKa = 3.4NTQYY115 pKa = 11.26TSFGVPTNGTGIDD128 pKa = 3.3IASNYY133 pKa = 9.63APYY136 pKa = 10.34ISANLASAGTQPGNGRR152 pKa = 11.84VKK154 pKa = 10.66LGEE157 pKa = 3.92LTITLSRR164 pKa = 11.84GNNNPILHH172 pKa = 6.32FKK174 pKa = 10.77GLGAASGSSLTAEE187 pKa = 4.49FNIKK191 pKa = 10.37SILNSAGAEE200 pKa = 3.89ILSTTNLVQLTGTNLNVNNITKK222 pKa = 10.17IINNDD227 pKa = 3.14YY228 pKa = 10.7TGATDD233 pKa = 3.76PATTNTARR241 pKa = 11.84GSVRR245 pKa = 11.84FQNNDD250 pKa = 1.76IRR252 pKa = 11.84TIVLEE257 pKa = 4.25VYY259 pKa = 10.57GNRR262 pKa = 11.84NGTASNVTWTGADD275 pKa = 3.53DD276 pKa = 3.99FFISISAGEE285 pKa = 4.45SDD287 pKa = 4.16LQVTKK292 pKa = 9.88TVSNATPTEE301 pKa = 3.86GSNIVFTVTASNLGASNNTNVTVNDD326 pKa = 4.81LLPTGYY332 pKa = 9.2TYY334 pKa = 11.25VSHH337 pKa = 6.38TASTGTYY344 pKa = 9.96VPGTGVWTIGNLNDD358 pKa = 4.13AANATLAITAKK369 pKa = 10.45VNPTGNFTNSATISTTSGILDD390 pKa = 4.14PNTSNNSASVSTTPVIVDD408 pKa = 3.38SDD410 pKa = 3.94GDD412 pKa = 4.1GVPNSLDD419 pKa = 4.27LDD421 pKa = 4.43DD422 pKa = 6.41DD423 pKa = 4.25NDD425 pKa = 5.19GITDD429 pKa = 3.68CDD431 pKa = 3.85EE432 pKa = 4.43NLLGANASVSNVFSFVTAGTAIATSNNEE460 pKa = 3.31VRR462 pKa = 11.84LTQNTGNQAGQIWSVGKK479 pKa = 10.73VDD481 pKa = 4.12FTKK484 pKa = 10.95SFVLRR489 pKa = 11.84FDD491 pKa = 4.75AYY493 pKa = 11.16LGTNDD498 pKa = 4.29NGADD502 pKa = 4.05GIAAVFHH509 pKa = 6.46NSPQGVNATGATGSGIGARR528 pKa = 11.84GIANGIVLEE537 pKa = 4.67LDD539 pKa = 3.46TYY541 pKa = 12.05DD542 pKa = 3.89NGTSLGDD549 pKa = 3.24ISSDD553 pKa = 3.44HH554 pKa = 5.55GQIWDD559 pKa = 4.03SDD561 pKa = 3.9NQTSTGFLTTAVALPNLEE579 pKa = 3.76NSTWHH584 pKa = 6.02PVVINWNASTKK595 pKa = 8.92TISYY599 pKa = 8.83TVDD602 pKa = 2.68GTNAGTYY609 pKa = 9.67TGDD612 pKa = 3.21LVTNYY617 pKa = 9.51FAGANKK623 pKa = 9.95VYY625 pKa = 10.47FGYY628 pKa = 8.5TASTGGATNEE638 pKa = 3.59QKK640 pKa = 10.35IRR642 pKa = 11.84INSFCSDD649 pKa = 4.17LPLEE653 pKa = 5.01LDD655 pKa = 3.51TDD657 pKa = 4.2GDD659 pKa = 4.58DD660 pKa = 4.12IANSLDD666 pKa = 3.97LDD668 pKa = 4.27SDD670 pKa = 4.02NDD672 pKa = 3.99GCPDD676 pKa = 4.7AIEE679 pKa = 4.46GDD681 pKa = 4.08EE682 pKa = 4.44NVTASQLTANRR693 pKa = 11.84ISGGVDD699 pKa = 3.07VNGVPNLVNSGGAADD714 pKa = 3.83IDD716 pKa = 4.03GDD718 pKa = 3.81QGQGAGQAYY727 pKa = 6.44TVNPAAAGGTASSNQSICTGATPAALSLSGHH758 pKa = 6.18SGAIQWQSSTDD769 pKa = 3.21NVTFTNISGATSTTYY784 pKa = 11.22APGALTATTYY794 pKa = 10.54YY795 pKa = 10.48RR796 pKa = 11.84AVLTSAGGCTANSSTVTITVNPLPTITGTTAVCVGSTTQLSGSGTPAASS845 pKa = 3.08

Molecular weight: 86.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5UAT1|A0A1E5UAT1_9FLAO Putative secreted protein OS=Cloacibacterium normanense OX=237258 GN=BHF72_0987 PE=4 SV=1
MM1 pKa = 7.49SIVHH5 pKa = 6.6KK6 pKa = 10.1IGRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 7.93TSVARR16 pKa = 11.84VYY18 pKa = 10.35VKK20 pKa = 10.51PGTGNITVNGKK31 pKa = 8.29DD32 pKa = 3.0AKK34 pKa = 10.26TYY36 pKa = 10.12FCTDD40 pKa = 2.94MLVYY44 pKa = 10.26KK45 pKa = 10.42VNQPFLLTEE54 pKa = 3.96TVGQYY59 pKa = 11.19DD60 pKa = 3.33VTVNVFGGGITGQAEE75 pKa = 4.93AIRR78 pKa = 11.84LGISRR83 pKa = 11.84ALCAINEE90 pKa = 4.15EE91 pKa = 4.09NRR93 pKa = 11.84LALKK97 pKa = 9.89PAGLLTRR104 pKa = 11.84DD105 pKa = 2.99ARR107 pKa = 11.84MVEE110 pKa = 4.26RR111 pKa = 11.84KK112 pKa = 9.98KK113 pKa = 10.51PGQKK117 pKa = 9.6KK118 pKa = 9.13ARR120 pKa = 11.84KK121 pKa = 8.69RR122 pKa = 11.84FQFSKK127 pKa = 10.78RR128 pKa = 3.42

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2558

0

2558

805349

37

2395

314.8

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.258 ± 0.058

0.706 ± 0.017

5.114 ± 0.034

7.235 ± 0.058

5.696 ± 0.047

6.166 ± 0.045

1.701 ± 0.023

7.944 ± 0.048

8.687 ± 0.063

9.16 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.024

6.483 ± 0.058

3.294 ± 0.023

3.572 ± 0.033

3.153 ± 0.023

6.143 ± 0.042

5.327 ± 0.051

6.059 ± 0.041

1.001 ± 0.019

4.046 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski