Blackbird associated gemycircularvirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus blabi1

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YSH3|T1YSH3_9VIRU Replication associated protein OS=Blackbird associated gemycircularvirus 1 OX=1985371 PE=3 SV=1
MM1 pKa = 7.78PPKK4 pKa = 10.2QKK6 pKa = 10.32PPYY9 pKa = 10.25ANFRR13 pKa = 11.84YY14 pKa = 10.22ALLTYY19 pKa = 8.84AQCGDD24 pKa = 3.48LDD26 pKa = 3.38AWAVNDD32 pKa = 3.52HH33 pKa = 6.0MAFLGAEE40 pKa = 4.26CIIGRR45 pKa = 11.84EE46 pKa = 4.02LHH48 pKa = 6.83ADD50 pKa = 3.72GGTHH54 pKa = 6.27LHH56 pKa = 6.45AFVDD60 pKa = 5.06FSRR63 pKa = 11.84RR64 pKa = 11.84FRR66 pKa = 11.84SRR68 pKa = 11.84RR69 pKa = 11.84SDD71 pKa = 3.3VFDD74 pKa = 3.2VGGRR78 pKa = 11.84HH79 pKa = 6.27PNLVPSYY86 pKa = 9.53GSPEE90 pKa = 3.9GGFDD94 pKa = 3.65YY95 pKa = 10.76AIKK98 pKa = 10.93DD99 pKa = 3.51GDD101 pKa = 4.03VVAGGLSRR109 pKa = 11.84PSGRR113 pKa = 11.84GVYY116 pKa = 10.06EE117 pKa = 4.89DD118 pKa = 3.53VSSWSIVVGQKK129 pKa = 10.15SEE131 pKa = 3.99GDD133 pKa = 3.08FWEE136 pKa = 4.99CVARR140 pKa = 11.84LDD142 pKa = 5.07PRR144 pKa = 11.84SLCTNYY150 pKa = 10.59NSLRR154 pKa = 11.84AYY156 pKa = 10.45ANWKK160 pKa = 8.61YY161 pKa = 10.96KK162 pKa = 9.6PVPVRR167 pKa = 11.84YY168 pKa = 7.77EE169 pKa = 3.82HH170 pKa = 6.55PAGVEE175 pKa = 4.15FEE177 pKa = 4.61LGMVPEE183 pKa = 4.27LAVWRR188 pKa = 11.84EE189 pKa = 3.5IALGANRR196 pKa = 11.84EE197 pKa = 3.94RR198 pKa = 11.84GILVIYY204 pKa = 10.51GDD206 pKa = 3.62TRR208 pKa = 11.84LGKK211 pKa = 7.48TLWSRR216 pKa = 11.84SLGNHH221 pKa = 7.07IYY223 pKa = 10.38TIGQMSGEE231 pKa = 4.29VILRR235 pKa = 11.84DD236 pKa = 3.59QADD239 pKa = 3.15AEE241 pKa = 4.52YY242 pKa = 11.0AVFDD246 pKa = 4.31DD247 pKa = 3.99MRR249 pKa = 11.84GGLEE253 pKa = 4.55FFHH256 pKa = 6.92GWKK259 pKa = 9.78EE260 pKa = 3.78WFGCQNVVTVKK271 pKa = 10.58KK272 pKa = 10.3LYY274 pKa = 10.03KK275 pKa = 10.65DD276 pKa = 3.6PVQMPWGRR284 pKa = 11.84PVIWLANRR292 pKa = 11.84DD293 pKa = 3.65PRR295 pKa = 11.84EE296 pKa = 3.9EE297 pKa = 4.06LRR299 pKa = 11.84DD300 pKa = 3.3GVTQHH305 pKa = 6.08TSMGKK310 pKa = 8.58QAAIEE315 pKa = 4.34GDD317 pKa = 3.96IKK319 pKa = 10.76WLDD322 pKa = 3.56GNCIFVEE329 pKa = 4.06LDD331 pKa = 3.07HH332 pKa = 7.33AIFRR336 pKa = 11.84ASTT339 pKa = 3.17

Molecular weight: 38.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YSH3|T1YSH3_9VIRU Replication associated protein OS=Blackbird associated gemycircularvirus 1 OX=1985371 PE=3 SV=1
MM1 pKa = 7.29VYY3 pKa = 9.92RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.25SSSSRR11 pKa = 11.84ARR13 pKa = 11.84KK14 pKa = 9.05PIGRR18 pKa = 11.84TGRR21 pKa = 11.84RR22 pKa = 11.84GGTRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84YY29 pKa = 5.73PTRR32 pKa = 11.84KK33 pKa = 7.68RR34 pKa = 11.84TYY36 pKa = 8.85RR37 pKa = 11.84KK38 pKa = 9.74NRR40 pKa = 11.84ALPKK44 pKa = 10.22KK45 pKa = 10.23RR46 pKa = 11.84ILNLTSRR53 pKa = 11.84KK54 pKa = 9.17KK55 pKa = 10.48RR56 pKa = 11.84NGMLTWTNTSSEE68 pKa = 4.29NGGSVTPGIGSLVVNARR85 pKa = 11.84DD86 pKa = 3.49GLEE89 pKa = 4.28MIFCPHH95 pKa = 5.92RR96 pKa = 11.84HH97 pKa = 5.38GSVFSSGTSTVAQVPLRR114 pKa = 11.84TATSCYY120 pKa = 9.21MRR122 pKa = 11.84GFAEE126 pKa = 4.51NIRR129 pKa = 11.84IQSSSGIPWFHH140 pKa = 6.58RR141 pKa = 11.84RR142 pKa = 11.84ICFTHH147 pKa = 7.16KK148 pKa = 10.15GDD150 pKa = 3.78APFRR154 pKa = 11.84EE155 pKa = 4.7LAAGDD160 pKa = 3.92TPVNTFSPLVEE171 pKa = 4.46TSNGMQRR178 pKa = 11.84LAFNLTINAQANTRR192 pKa = 11.84NAMKK196 pKa = 10.57NYY198 pKa = 9.54LFKK201 pKa = 10.87GAQGRR206 pKa = 11.84DD207 pKa = 3.08WNNLIVAPVDD217 pKa = 3.45TSRR220 pKa = 11.84VTLKK224 pKa = 10.48FDD226 pKa = 3.35KK227 pKa = 10.06TWTYY231 pKa = 11.33RR232 pKa = 11.84SGNANGMVKK241 pKa = 8.37EE242 pKa = 4.53TKK244 pKa = 9.96LFHH247 pKa = 6.9PMNQTLVYY255 pKa = 10.75DD256 pKa = 4.32DD257 pKa = 5.56DD258 pKa = 4.69EE259 pKa = 6.53NGDD262 pKa = 3.86IEE264 pKa = 4.22QDD266 pKa = 3.09SYY268 pKa = 12.19YY269 pKa = 11.16SVASKK274 pKa = 11.13AGMGDD279 pKa = 4.03YY280 pKa = 11.09YY281 pKa = 11.69VMDD284 pKa = 5.39FIQPGLGGSVTDD296 pKa = 4.35LLAMHH301 pKa = 7.04FNSTMYY307 pKa = 8.7WHH309 pKa = 6.77EE310 pKa = 3.98RR311 pKa = 3.42

Molecular weight: 35.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

650

311

339

325.0

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.557

1.385 ± 0.288

5.846 ± 0.92

4.462 ± 1.073

4.308 ± 0.087

9.538 ± 0.587

2.615 ± 0.25

4.154 ± 0.202

4.462 ± 0.468

6.769 ± 0.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.64

4.923 ± 1.252

4.615 ± 0.298

2.615 ± 0.029

8.769 ± 0.821

6.769 ± 1.089

5.692 ± 2.047

6.769 ± 0.892

2.462 ± 0.585

4.0 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski