Romboutsia sp. CE17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Romboutsia; unclassified Romboutsia

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H1WZ53|A0A6H1WZ53_9FIRM MarR family transcriptional regulator OS=Romboutsia sp. CE17 OX=2724150 GN=HF520_05160 PE=4 SV=1
MM1 pKa = 7.06EE2 pKa = 6.68AIRR5 pKa = 11.84INPEE9 pKa = 3.47EE10 pKa = 3.9FKK12 pKa = 11.05LINFINYY19 pKa = 9.59YY20 pKa = 8.92NDD22 pKa = 3.13NYY24 pKa = 11.07EE25 pKa = 4.25EE26 pKa = 4.43LLSDD30 pKa = 3.86FPNYY34 pKa = 10.0VSRR37 pKa = 11.84ICLIDD42 pKa = 4.07KK43 pKa = 10.52DD44 pKa = 4.27YY45 pKa = 10.96MDD47 pKa = 4.24VVTFDD52 pKa = 3.37EE53 pKa = 5.54DD54 pKa = 4.21YY55 pKa = 11.6EE56 pKa = 4.22EE57 pKa = 5.53LEE59 pKa = 4.13NAHH62 pKa = 7.05DD63 pKa = 4.21YY64 pKa = 11.18EE65 pKa = 4.52SLLLNEE71 pKa = 4.94EE72 pKa = 4.35YY73 pKa = 10.82ALHH76 pKa = 6.03FVIGRR81 pKa = 11.84TDD83 pKa = 3.22EE84 pKa = 4.13NLEE87 pKa = 4.05SVEE90 pKa = 4.82FIDD93 pKa = 6.46GEE95 pKa = 4.65TKK97 pKa = 10.43SLKK100 pKa = 10.76NYY102 pKa = 9.97VDD104 pKa = 4.95DD105 pKa = 4.68IYY107 pKa = 11.19EE108 pKa = 4.07EE109 pKa = 4.59SSIKK113 pKa = 10.69DD114 pKa = 3.19IGDD117 pKa = 3.73LNLDD121 pKa = 3.81LNHH124 pKa = 6.95LVGLLLDD131 pKa = 4.38FEE133 pKa = 5.67DD134 pKa = 4.26NEE136 pKa = 4.41IVISVVNFEE145 pKa = 4.74HH146 pKa = 7.08GGEE149 pKa = 3.88LSMPRR154 pKa = 11.84IIEE157 pKa = 3.93VDD159 pKa = 3.28DD160 pKa = 4.91CGDD163 pKa = 3.46LEE165 pKa = 4.27EE166 pKa = 4.84TIRR169 pKa = 11.84ALVNRR174 pKa = 11.84FTAA177 pKa = 3.89

Molecular weight: 20.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H1X379|A0A6H1X379_9FIRM 50S ribosomal protein L10 OS=Romboutsia sp. CE17 OX=2724150 GN=rplJ PE=3 SV=1
MM1 pKa = 7.67ANYY4 pKa = 9.66RR5 pKa = 11.84KK6 pKa = 9.97LGRR9 pKa = 11.84VTSHH13 pKa = 6.74RR14 pKa = 11.84NLMLRR19 pKa = 11.84NLVTDD24 pKa = 4.18LLRR27 pKa = 11.84CGRR30 pKa = 11.84IEE32 pKa = 4.23TTVTRR37 pKa = 11.84AKK39 pKa = 8.01EE40 pKa = 4.1TRR42 pKa = 11.84RR43 pKa = 11.84MAEE46 pKa = 3.94KK47 pKa = 10.02MITLAKK53 pKa = 10.41RR54 pKa = 11.84GDD56 pKa = 3.38LHH58 pKa = 7.54ARR60 pKa = 11.84RR61 pKa = 11.84QVLAYY66 pKa = 11.02VMDD69 pKa = 3.7EE70 pKa = 4.19TVVNNLFTDD79 pKa = 3.95IAPKK83 pKa = 9.34YY84 pKa = 9.57AEE86 pKa = 4.25RR87 pKa = 11.84NGGYY91 pKa = 7.95TRR93 pKa = 11.84IIKK96 pKa = 9.98KK97 pKa = 10.06GPRR100 pKa = 11.84RR101 pKa = 11.84GDD103 pKa = 3.28AAEE106 pKa = 3.81MAFIEE111 pKa = 4.79LVV113 pKa = 3.2

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2826

0

2826

850299

31

2122

300.9

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.244 ± 0.057

1.21 ± 0.022

5.811 ± 0.039

7.497 ± 0.051

4.176 ± 0.036

6.253 ± 0.049

1.34 ± 0.017

10.463 ± 0.064

8.991 ± 0.052

9.038 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.022

6.653 ± 0.047

2.664 ± 0.024

2.163 ± 0.02

3.156 ± 0.032

6.568 ± 0.039

4.818 ± 0.035

6.447 ± 0.043

0.573 ± 0.013

4.308 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski