Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3233 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6AKS0|Q6AKS0_DESPS Uncharacterized protein OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=DP2326 PE=4 SV=1
MM1 pKa = 7.73 GIIDD5 pKa = 4.08 MEE7 pKa = 4.02 ILYY10 pKa = 10.71 VIGNGFDD17 pKa = 3.25 LWHH20 pKa = 6.69 GLPTKK25 pKa = 10.0 YY26 pKa = 9.3 TDD28 pKa = 4.68 FYY30 pKa = 11.41 AFSEE34 pKa = 4.29 THH36 pKa = 6.66 LDD38 pKa = 3.36 EE39 pKa = 5.65 LEE41 pKa = 4.1 QYY43 pKa = 11.1 LCFDD47 pKa = 5.7 IITEE51 pKa = 4.17 NPWNNFEE58 pKa = 4.22 VVLGKK63 pKa = 10.11 FDD65 pKa = 3.92 WEE67 pKa = 3.81 IFYY70 pKa = 11.12 NNHH73 pKa = 5.46 NFIDD77 pKa = 3.86 VSDD80 pKa = 4.8 DD81 pKa = 3.43 SFKK84 pKa = 11.24 PSMAYY89 pKa = 10.27 GLEE92 pKa = 4.17 DD93 pKa = 5.68 DD94 pKa = 5.12 IKK96 pKa = 10.4 EE97 pKa = 4.14 QADD100 pKa = 3.75 NLVDD104 pKa = 5.64 DD105 pKa = 4.49 IQQ107 pKa = 5.32
Molecular weight: 12.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|Q6ANB4|Q6ANB4_DESPS DNA polymerase III subunit gamma/tau OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=dnaX PE=3 SV=1
MM1 pKa = 7.36 TLFAFQLRR9 pKa = 11.84 RR10 pKa = 11.84 STISIIYY17 pKa = 10.07 ALIAHH22 pKa = 6.95 IRR24 pKa = 11.84 RR25 pKa = 11.84 PNSTGQPATRR35 pKa = 11.84 QFQSLSRR42 pKa = 11.84 PLIHH46 pKa = 7.17 RR47 pKa = 11.84 KK48 pKa = 7.33 KK49 pKa = 9.07 TRR51 pKa = 11.84 PHH53 pKa = 6.27 RR54 pKa = 11.84 NKK56 pKa = 10.42 LLQTNRR62 pKa = 11.84 GGSSSRR68 pKa = 11.84 RR69 pKa = 3.57
Molecular weight: 8.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3233
0
3233
1036698
31
4725
320.7
35.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.533 ± 0.049
1.385 ± 0.026
5.077 ± 0.049
6.336 ± 0.051
4.306 ± 0.037
7.521 ± 0.064
1.986 ± 0.023
7.138 ± 0.043
5.699 ± 0.047
10.397 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.571 ± 0.027
3.781 ± 0.042
3.921 ± 0.034
3.723 ± 0.03
5.01 ± 0.044
6.628 ± 0.046
5.295 ± 0.056
6.665 ± 0.046
1.022 ± 0.016
3.006 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here