Maribacter sp. (strain HTCC2170 / KCCM 42371)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Maribacter; unclassified Maribacter

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4AT64|A4AT64_MARSH Uncharacterized protein OS=Maribacter sp. (strain HTCC2170 / KCCM 42371) OX=313603 GN=FB2170_16156 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84NSQSFLSKK11 pKa = 9.81LTFGIILSLLIFYY24 pKa = 10.45HH25 pKa = 7.01GEE27 pKa = 4.13TFSQTTFTEE36 pKa = 4.1SAAAYY41 pKa = 9.39GLNLGGNKK49 pKa = 10.19DD50 pKa = 4.28GGHH53 pKa = 6.48AWADD57 pKa = 3.38YY58 pKa = 11.38DD59 pKa = 5.18LDD61 pKa = 4.87GDD63 pKa = 3.98FDD65 pKa = 5.18LVVNTQGNGKK75 pKa = 10.14LYY77 pKa = 10.68RR78 pKa = 11.84NDD80 pKa = 3.48GGSFTDD86 pKa = 3.38VTGTLAPDD94 pKa = 4.04FNGGSLEE101 pKa = 4.09RR102 pKa = 11.84TVLFVDD108 pKa = 4.95FNNDD112 pKa = 2.65GYY114 pKa = 10.47PDD116 pKa = 3.59VFRR119 pKa = 11.84NKK121 pKa = 10.39HH122 pKa = 5.12NDD124 pKa = 2.95LRR126 pKa = 11.84IYY128 pKa = 10.64LQDD131 pKa = 3.61PATNRR136 pKa = 11.84FGNGTGGTVPNQRR149 pKa = 11.84FTSMTDD155 pKa = 3.01GMNTEE160 pKa = 4.4GAGALDD166 pKa = 3.82YY167 pKa = 11.58DD168 pKa = 4.48GDD170 pKa = 4.06GDD172 pKa = 5.35LDD174 pKa = 4.52LFVDD178 pKa = 3.6NHH180 pKa = 6.08NFGIDD185 pKa = 2.86ILQNDD190 pKa = 3.94GTGNFTHH197 pKa = 6.0VTRR200 pKa = 11.84KK201 pKa = 9.87ADD203 pKa = 3.86SPNPPYY209 pKa = 10.76DD210 pKa = 3.69VLNSTTWPLGLVQDD224 pKa = 4.57ATDD227 pKa = 3.75GDD229 pKa = 4.43YY230 pKa = 11.73GSATDD235 pKa = 4.66FNDD238 pKa = 4.31DD239 pKa = 3.06GWVDD243 pKa = 2.99IVVRR247 pKa = 11.84KK248 pKa = 9.7RR249 pKa = 11.84DD250 pKa = 3.67QVDD253 pKa = 3.68LFTNVGGTFQNGVNIDD269 pKa = 3.74DD270 pKa = 4.7ANNNNKK276 pKa = 9.7GAVAFYY282 pKa = 11.03DD283 pKa = 3.87FDD285 pKa = 4.46NDD287 pKa = 4.64GDD289 pKa = 4.1FDD291 pKa = 4.28MYY293 pKa = 8.96WTEE296 pKa = 3.79NGNNQIHH303 pKa = 6.81RR304 pKa = 11.84NNGDD308 pKa = 3.63GTWTGLGAATGIPISFSGQIEE329 pKa = 4.09GLACGDD335 pKa = 3.56VDD337 pKa = 4.26NDD339 pKa = 3.4GDD341 pKa = 4.12IDD343 pKa = 3.65IFLAGGANKK352 pKa = 10.33LFLNQINNGGGAMSFVDD369 pKa = 4.36SGLTFSNSGGEE380 pKa = 4.09GCTFIDD386 pKa = 3.97IDD388 pKa = 4.23QDD390 pKa = 3.5GDD392 pKa = 3.58LDD394 pKa = 4.95LYY396 pKa = 9.76TNRR399 pKa = 11.84SGNNRR404 pKa = 11.84LYY406 pKa = 10.64INNLMANQANHH417 pKa = 6.79LYY419 pKa = 10.16IDD421 pKa = 3.48IVEE424 pKa = 4.59DD425 pKa = 3.59RR426 pKa = 11.84DD427 pKa = 3.81AFGLINTEE435 pKa = 3.63EE436 pKa = 4.38RR437 pKa = 11.84FGVGATAKK445 pKa = 10.58ILDD448 pKa = 4.02CDD450 pKa = 4.14GNVISGTRR458 pKa = 11.84EE459 pKa = 3.64VNGGFGHH466 pKa = 6.6GTQGPGRR473 pKa = 11.84IHH475 pKa = 6.97FGLPSGPLTPIVVEE489 pKa = 4.01VSFPRR494 pKa = 11.84TASGRR499 pKa = 11.84VVVRR503 pKa = 11.84QQLIPNDD510 pKa = 3.84YY511 pKa = 11.13NNGSINLLDD520 pKa = 4.11VFPDD524 pKa = 3.77SANQPPTATDD534 pKa = 3.35EE535 pKa = 4.33YY536 pKa = 8.47TTVVEE541 pKa = 4.25NSFVSLDD548 pKa = 3.69PLIDD552 pKa = 3.8NGNGADD558 pKa = 4.14SDD560 pKa = 4.48PEE562 pKa = 4.34GEE564 pKa = 4.08PLEE567 pKa = 4.74VISVTQPSNGVSVLNGDD584 pKa = 3.57GTVTYY589 pKa = 9.85TPTTGFFGNDD599 pKa = 2.6SFTYY603 pKa = 9.73TMRR606 pKa = 11.84DD607 pKa = 3.17NANCTFTSEE616 pKa = 4.11EE617 pKa = 4.2ATGSIYY623 pKa = 9.65ITVFPDD629 pKa = 3.14SDD631 pKa = 3.84NDD633 pKa = 3.91TVADD637 pKa = 4.9RR638 pKa = 11.84VDD640 pKa = 4.93LDD642 pKa = 4.5DD643 pKa = 6.95DD644 pKa = 4.5NDD646 pKa = 4.03GLLDD650 pKa = 4.24ADD652 pKa = 4.5EE653 pKa = 5.37LNTIISNSQQDD664 pKa = 4.01CTGEE668 pKa = 4.13TTLDD672 pKa = 3.6FSSAATLVSGTALQLGAVYY691 pKa = 10.25RR692 pKa = 11.84ISNITTGTDD701 pKa = 2.97ALVTIDD707 pKa = 3.3QTFNATVANIDD718 pKa = 3.74NNGSEE723 pKa = 4.27PASFRR728 pKa = 11.84PQTAFNLTNIGDD740 pKa = 3.55QGYY743 pKa = 9.61IEE745 pKa = 4.29YY746 pKa = 10.35RR747 pKa = 11.84IQFVNSGGSAPVVISKK763 pKa = 9.35FFTNFNDD770 pKa = 3.18IDD772 pKa = 3.99GNSNYY777 pKa = 10.27GEE779 pKa = 4.55QIWSDD784 pKa = 3.43NPTSYY789 pKa = 10.47IISNPTEE796 pKa = 3.96LTMSTDD802 pKa = 3.7GSWVLGTAGINEE814 pKa = 4.17FPGAGNTFPEE824 pKa = 4.2VNYY827 pKa = 10.13GVNYY831 pKa = 10.17NSQSEE836 pKa = 3.82ISIRR840 pKa = 11.84VGAVARR846 pKa = 11.84VAGASASGRR855 pKa = 11.84QHH857 pKa = 6.64NIEE860 pKa = 4.51FSCLTNYY867 pKa = 9.38VNPEE871 pKa = 3.95TYY873 pKa = 10.77GIDD876 pKa = 3.55SDD878 pKa = 4.15SDD880 pKa = 3.95GIANHH885 pKa = 7.19LDD887 pKa = 3.6LDD889 pKa = 4.02SDD891 pKa = 3.75NDD893 pKa = 4.55GIYY896 pKa = 10.79DD897 pKa = 3.6AVEE900 pKa = 4.29AGHH903 pKa = 5.97SQSHH907 pKa = 6.05SNGVVNGPYY916 pKa = 9.94GANGLANVVEE926 pKa = 4.67TAAEE930 pKa = 4.07SGAINYY936 pKa = 7.6TILDD940 pKa = 3.63TDD942 pKa = 3.76GTDD945 pKa = 3.41PADD948 pKa = 4.76FLDD951 pKa = 4.35TDD953 pKa = 4.45SDD955 pKa = 4.97DD956 pKa = 5.67DD957 pKa = 4.24GCSDD961 pKa = 5.06ANEE964 pKa = 4.77AYY966 pKa = 10.92NNANADD972 pKa = 3.64GGDD975 pKa = 3.49NEE977 pKa = 5.0YY978 pKa = 10.86YY979 pKa = 10.05ATGNPPATDD988 pKa = 3.11GFGRR992 pKa = 11.84VTGATYY998 pKa = 9.05PVPADD1003 pKa = 3.21IDD1005 pKa = 3.63TDD1007 pKa = 3.61STYY1010 pKa = 11.92DD1011 pKa = 3.38HH1012 pKa = 7.18LEE1014 pKa = 3.93VGLAPSISTQPPNTNVCPGCDD1035 pKa = 2.81ITIEE1039 pKa = 4.36VIASNSDD1046 pKa = 3.31TYY1048 pKa = 11.04QWQLDD1053 pKa = 4.14DD1054 pKa = 3.92GSGWVDD1060 pKa = 3.52LTDD1063 pKa = 3.3SGIYY1067 pKa = 9.95SDD1069 pKa = 4.14TSTSILTITNATAPDD1084 pKa = 3.57NGNEE1088 pKa = 3.72YY1089 pKa = 10.52RR1090 pKa = 11.84VIVSNTMFICSSEE1103 pKa = 4.19TSNSAVLTLRR1113 pKa = 11.84VNTIITNRR1121 pKa = 11.84RR1122 pKa = 11.84ITHH1125 pKa = 6.35RR1126 pKa = 11.84VNKK1129 pKa = 9.94NN1130 pKa = 2.67

Molecular weight: 120.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4AUD4|A4AUD4_MARSH Uncharacterized protein OS=Maribacter sp. (strain HTCC2170 / KCCM 42371) OX=313603 GN=FB2170_08769 PE=4 SV=1
MM1 pKa = 7.62PKK3 pKa = 10.27SNRR6 pKa = 11.84SRR8 pKa = 11.84SWIDD12 pKa = 2.94ILSEE16 pKa = 4.26LIEE19 pKa = 4.31VFFKK23 pKa = 10.97SAGRR27 pKa = 11.84SKK29 pKa = 10.72RR30 pKa = 11.84GRR32 pKa = 11.84TWHH35 pKa = 4.78QHH37 pKa = 3.44VVPYY41 pKa = 10.44EE42 pKa = 4.27DD43 pKa = 3.53DD44 pKa = 3.27WAVRR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 4.02GNKK53 pKa = 10.25RR54 pKa = 11.84ITSKK58 pKa = 10.32HH59 pKa = 5.07RR60 pKa = 11.84RR61 pKa = 11.84QDD63 pKa = 3.24TAIRR67 pKa = 11.84KK68 pKa = 9.06AKK70 pKa = 9.3TLARR74 pKa = 11.84KK75 pKa = 9.3HH76 pKa = 5.81KK77 pKa = 10.31ADD79 pKa = 4.04VIIHH83 pKa = 6.58RR84 pKa = 11.84SDD86 pKa = 2.73GTIRR90 pKa = 11.84DD91 pKa = 4.15RR92 pKa = 11.84INYY95 pKa = 8.01DD96 pKa = 2.82

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1168930

22

5669

342.7

38.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.265 ± 0.041

0.75 ± 0.017

5.794 ± 0.048

6.67 ± 0.046

5.117 ± 0.037

6.937 ± 0.066

1.874 ± 0.024

7.727 ± 0.041

7.626 ± 0.068

9.23 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.025

5.946 ± 0.038

3.555 ± 0.029

3.18 ± 0.021

3.508 ± 0.031

6.484 ± 0.031

5.552 ± 0.063

6.286 ± 0.033

1.177 ± 0.018

3.92 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski