Oribacterium sp. oral taxon 078 str. F0262
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4CPC5|D4CPC5_9FIRM Uncharacterized protein OS=Oribacterium sp. oral taxon 078 str. F0262 OX=608534 GN=GCWU000341_02216 PE=4 SV=1
MM1 pKa = 7.59 TDD3 pKa = 3.09 YY4 pKa = 11.24 AALVGLEE11 pKa = 4.31 FYY13 pKa = 10.91 FLAADD18 pKa = 4.23 TEE20 pKa = 4.75 PGFSGEE26 pKa = 3.66 INAADD31 pKa = 3.86 FQKK34 pKa = 9.96 TVIDD38 pKa = 4.46 LVSFHH43 pKa = 7.28 CISVHH48 pKa = 6.92 PDD50 pKa = 2.7 TT51 pKa = 5.66
Molecular weight: 5.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 4.012
IPC_protein 3.846
Toseland 3.656
ProMoST 4.05
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.592
Solomon 3.821
Lehninger 3.783
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.745
EMBOSS 3.834
Sillero 3.973
Patrickios 0.604
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|D4CN60|D4CN60_9FIRM Uncharacterized protein OS=Oribacterium sp. oral taxon 078 str. F0262 OX=608534 GN=GCWU000341_01800 PE=4 SV=1
MM1 pKa = 7.36 HH2 pKa = 7.48 LSLGRR7 pKa = 11.84 GRR9 pKa = 11.84 QNGSAFFRR17 pKa = 11.84 TRR19 pKa = 11.84 GGRR22 pKa = 11.84 QSGSASFRR30 pKa = 11.84 RR31 pKa = 11.84 IKK33 pKa = 10.15 KK34 pKa = 9.13 GKK36 pKa = 9.86 RR37 pKa = 11.84 SGGANGLPRR46 pKa = 11.84 GGG48 pKa = 3.59
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.544
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.281
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2936
0
2936
809676
39
2318
275.8
30.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.836 ± 0.049
1.424 ± 0.019
5.021 ± 0.031
8.528 ± 0.068
4.343 ± 0.034
7.875 ± 0.048
1.65 ± 0.02
6.295 ± 0.042
5.773 ± 0.04
10.574 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.023
3.186 ± 0.029
3.857 ± 0.026
2.708 ± 0.026
7.159 ± 0.055
7.152 ± 0.045
4.054 ± 0.033
5.419 ± 0.038
0.905 ± 0.019
3.567 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here