Natronolimnohabitans innermongolicus JCM 12255

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natronolimnohabitans; Natronolimnohabitans innermongolicus

Average proteome isoelectric point is 4.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L9WKT0|L9WKT0_9EURY Uncharacterized protein OS=Natronolimnohabitans innermongolicus JCM 12255 OX=1227499 GN=C493_19771 PE=4 SV=1
MM1 pKa = 7.01ATAATSLGLVGSASAQDD18 pKa = 3.64DD19 pKa = 4.45YY20 pKa = 11.98EE21 pKa = 4.59VIEE24 pKa = 4.94ASGQSITVADD34 pKa = 4.06GEE36 pKa = 4.43SWEE39 pKa = 4.34NKK41 pKa = 9.78LIDD44 pKa = 3.76MTTGQDD50 pKa = 3.41VSITTTGSDD59 pKa = 2.77WTIRR63 pKa = 11.84NVGFHH68 pKa = 6.16GRR70 pKa = 11.84NEE72 pKa = 4.35SGAGTATFAISDD84 pKa = 3.8AGGEE88 pKa = 4.22STIEE92 pKa = 3.69NVYY95 pKa = 10.83LGDD98 pKa = 4.71GSDD101 pKa = 4.15DD102 pKa = 4.06RR103 pKa = 11.84NGSSTGHH110 pKa = 6.02GQTAFWVNPDD120 pKa = 3.48HH121 pKa = 7.21AGHH124 pKa = 7.42IDD126 pKa = 3.54MQNVNIQGFADD137 pKa = 3.51NAVYY141 pKa = 10.46GSAPGNGGGGTIHH154 pKa = 7.32IDD156 pKa = 3.02SCFAANCYY164 pKa = 9.69VSHH167 pKa = 7.0FRR169 pKa = 11.84LATEE173 pKa = 4.74GSKK176 pKa = 8.69VTNSSILVDD185 pKa = 3.67DD186 pKa = 4.35EE187 pKa = 5.48GYY189 pKa = 10.76AGRR192 pKa = 11.84GIWAWAPGTIEE203 pKa = 4.79VEE205 pKa = 3.97NCQIEE210 pKa = 4.48MNGNHH215 pKa = 5.55TAIDD219 pKa = 3.57AGANGQGTQVVVADD233 pKa = 3.85TDD235 pKa = 3.69YY236 pKa = 11.85DD237 pKa = 3.89EE238 pKa = 4.35QAGIAEE244 pKa = 4.38HH245 pKa = 6.73AGSNVQLEE253 pKa = 4.78GDD255 pKa = 3.6TGTDD259 pKa = 3.4PEE261 pKa = 5.28AIIPDD266 pKa = 4.05GTPTSAEE273 pKa = 3.61AAAAGDD279 pKa = 4.01DD280 pKa = 3.62

Molecular weight: 28.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L9WTT5|L9WTT5_9EURY Gas vesicle structural protein OS=Natronolimnohabitans innermongolicus JCM 12255 OX=1227499 GN=gvpA PE=3 SV=1
MM1 pKa = 7.42SVLGLRR7 pKa = 11.84PRR9 pKa = 11.84VKK11 pKa = 10.5AALLWGAVGSMAFLVLVQGYY31 pKa = 9.83ALLVDD36 pKa = 4.41PLVSLARR43 pKa = 11.84GAAIAVLVGAMTTGCAYY60 pKa = 10.63RR61 pKa = 11.84LEE63 pKa = 4.35HH64 pKa = 7.15RR65 pKa = 11.84VAEE68 pKa = 4.32WAARR72 pKa = 11.84RR73 pKa = 11.84ARR75 pKa = 11.84RR76 pKa = 11.84GDD78 pKa = 3.33EE79 pKa = 3.87SHH81 pKa = 6.72GVDD84 pKa = 4.3GKK86 pKa = 11.32SKK88 pKa = 10.54SS89 pKa = 3.38

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4400

0

4400

1253988

28

2952

285.0

31.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.782 ± 0.055

0.755 ± 0.011

8.99 ± 0.053

9.283 ± 0.053

3.208 ± 0.022

8.294 ± 0.034

1.964 ± 0.017

4.295 ± 0.029

1.632 ± 0.022

8.909 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.017

2.193 ± 0.021

4.542 ± 0.026

2.364 ± 0.024

6.546 ± 0.039

5.571 ± 0.034

6.431 ± 0.027

8.747 ± 0.037

1.115 ± 0.014

2.687 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski