Amino acid dipepetide frequency for Natronolimnohabitans innermongolicus JCM 12255

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.114AlaAla: 13.114 ± 0.159	0.704AlaCys: 0.704 ± 0.022	10.635AlaAsp: 10.635 ± 0.111	8.526AlaGlu: 8.526 ± 0.091	3.843AlaPhe: 3.843 ± 0.057	9.129AlaGly: 9.129 ± 0.092	1.719AlaHis: 1.719 ± 0.04	5.518AlaIle: 5.518 ± 0.076	1.719AlaLys: 1.719 ± 0.043	10.358AlaLeu: 10.358 ± 0.123	2.065AlaMet: 2.065 ± 0.048	2.348AlaAsn: 2.348 ± 0.038	3.723AlaPro: 3.723 ± 0.065	2.09AlaGln: 2.09 ± 0.046	5.752AlaArg: 5.752 ± 0.075	5.378AlaSer: 5.378 ± 0.064	7.266AlaThr: 7.266 ± 0.073	10.188AlaVal: 10.188 ± 0.123	1.02AlaTrp: 1.02 ± 0.029	2.726AlaTyr: 2.726 ± 0.045	0.001AlaXaa: 0.001 ± 0.001
Cys	0.624CysAla: 0.624 ± 0.024	0.131CysCys: 0.131 ± 0.01	0.632CysAsp: 0.632 ± 0.023	0.648CysGlu: 0.648 ± 0.024	0.226CysPhe: 0.226 ± 0.015	0.821CysGly: 0.821 ± 0.027	0.201CysHis: 0.201 ± 0.014	0.263CysIle: 0.263 ± 0.015	0.131CysLys: 0.131 ± 0.01	0.648CysLeu: 0.648 ± 0.023	0.101CysMet: 0.101 ± 0.01	0.192CysAsn: 0.192 ± 0.013	0.495CysPro: 0.495 ± 0.022	0.167CysGln: 0.167 ± 0.013	0.516CysArg: 0.516 ± 0.02	0.467CysSer: 0.467 ± 0.02	0.437CysThr: 0.437 ± 0.019	0.5CysVal: 0.5 ± 0.02	0.101CysTrp: 0.101 ± 0.009	0.252CysTyr: 0.252 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	10.803AspAla: 10.803 ± 0.118	0.715AspCys: 0.715 ± 0.027	10.369AspAsp: 10.369 ± 0.169	9.979AspGlu: 9.979 ± 0.122	1.82AspPhe: 1.82 ± 0.035	8.856AspGly: 8.856 ± 0.11	1.935AspHis: 1.935 ± 0.038	2.671AspIle: 2.671 ± 0.049	0.79AspLys: 0.79 ± 0.026	7.369AspLeu: 7.369 ± 0.105	1.151AspMet: 1.151 ± 0.032	1.182AspAsn: 1.182 ± 0.031	4.589AspPro: 4.589 ± 0.06	1.691AspGln: 1.691 ± 0.039	7.095AspArg: 7.095 ± 0.081	4.036AspSer: 4.036 ± 0.062	3.624AspThr: 3.624 ± 0.059	8.329AspVal: 8.329 ± 0.097	1.037AspTrp: 1.037 ± 0.031	1.856AspTyr: 1.856 ± 0.04	0.002AspXaa: 0.002 ± 0.002
Glu	9.007GluAla: 9.007 ± 0.09	0.564GluCys: 0.564 ± 0.021	6.658GluAsp: 6.658 ± 0.098	7.085GluGlu: 7.085 ± 0.116	3.226GluPhe: 3.226 ± 0.053	5.642GluGly: 5.642 ± 0.083	2.095GluHis: 2.095 ± 0.046	4.557GluIle: 4.557 ± 0.072	1.78GluLys: 1.78 ± 0.048	8.205GluLeu: 8.205 ± 0.084	1.88GluMet: 1.88 ± 0.038	2.552GluAsn: 2.552 ± 0.052	4.794GluPro: 4.794 ± 0.075	2.754GluGln: 2.754 ± 0.054	8.525GluArg: 8.525 ± 0.103	6.08GluSer: 6.08 ± 0.083	7.88GluThr: 7.88 ± 0.09	5.272GluVal: 5.272 ± 0.068	1.209GluTrp: 1.209 ± 0.033	3.061GluTyr: 3.061 ± 0.054	0.002GluXaa: 0.002 ± 0.001
Phe	3.658PheAla: 3.658 ± 0.062	0.282PheCys: 0.282 ± 0.016	3.418PheAsp: 3.418 ± 0.056	3.237PheGlu: 3.237 ± 0.059	0.967PhePhe: 0.967 ± 0.03	3.129PheGly: 3.129 ± 0.055	0.654PheHis: 0.654 ± 0.023	1.005PheIle: 1.005 ± 0.034	0.4PheLys: 0.4 ± 0.015	2.671PheLeu: 2.671 ± 0.053	0.489PheMet: 0.489 ± 0.021	0.616PheAsn: 0.616 ± 0.02	1.249PhePro: 1.249 ± 0.031	0.723PheGln: 0.723 ± 0.025	1.675PheArg: 1.675 ± 0.037	1.57PheSer: 1.57 ± 0.037	1.734PheThr: 1.734 ± 0.042	3.331PheVal: 3.331 ± 0.055	0.405PheTrp: 0.405 ± 0.019	0.867PheTyr: 0.867 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	8.114GlyAla: 8.114 ± 0.096	0.775GlyCys: 0.775 ± 0.026	7.372GlyAsp: 7.372 ± 0.092	7.12GlyGlu: 7.12 ± 0.083	2.945GlyPhe: 2.945 ± 0.054	6.854GlyGly: 6.854 ± 0.101	1.585GlyHis: 1.585 ± 0.036	4.227GlyIle: 4.227 ± 0.061	1.616GlyLys: 1.616 ± 0.042	7.36GlyLeu: 7.36 ± 0.087	1.578GlyMet: 1.578 ± 0.036	1.76GlyAsn: 1.76 ± 0.038	3.268GlyPro: 3.268 ± 0.061	1.861GlyGln: 1.861 ± 0.038	4.752GlyArg: 4.752 ± 0.074	5.124GlySer: 5.124 ± 0.077	5.857GlyThr: 5.857 ± 0.08	7.014GlyVal: 7.014 ± 0.084	1.11GlyTrp: 1.11 ± 0.031	2.64GlyTyr: 2.64 ± 0.046	0.002GlyXaa: 0.002 ± 0.001
His	1.941HisAla: 1.941 ± 0.046	0.217HisCys: 0.217 ± 0.013	1.903HisAsp: 1.903 ± 0.047	1.746HisGlu: 1.746 ± 0.033	0.518HisPhe: 0.518 ± 0.019	2.017HisGly: 2.017 ± 0.037	0.508HisHis: 0.508 ± 0.021	0.651HisIle: 0.651 ± 0.025	0.289HisLys: 0.289 ± 0.015	1.656HisLeu: 1.656 ± 0.039	0.285HisMet: 0.285 ± 0.015	0.403HisAsn: 0.403 ± 0.018	1.171HisPro: 1.171 ± 0.034	0.478HisGln: 0.478 ± 0.021	1.297HisArg: 1.297 ± 0.033	0.859HisSer: 0.859 ± 0.027	1.022HisThr: 1.022 ± 0.031	1.872HisVal: 1.872 ± 0.036	0.241HisTrp: 0.241 ± 0.017	0.565HisTyr: 0.565 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	5.184IleAla: 5.184 ± 0.069	0.293IleCys: 0.293 ± 0.016	4.691IleAsp: 4.691 ± 0.067	4.36IleGlu: 4.36 ± 0.078	1.045IlePhe: 1.045 ± 0.03	4.131IleGly: 4.131 ± 0.069	0.835IleHis: 0.835 ± 0.024	1.363IleIle: 1.363 ± 0.042	0.619IleLys: 0.619 ± 0.024	3.071IleLeu: 3.071 ± 0.059	0.529IleMet: 0.529 ± 0.022	0.932IleAsn: 0.932 ± 0.03	2.045IlePro: 2.045 ± 0.046	1.036IleGln: 1.036 ± 0.033	2.531IleArg: 2.531 ± 0.043	2.167IleSer: 2.167 ± 0.045	2.502IleThr: 2.502 ± 0.047	4.265IleVal: 4.265 ± 0.06	0.37IleTrp: 0.37 ± 0.016	0.981IleTyr: 0.981 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	1.541LysAla: 1.541 ± 0.043	0.101LysCys: 0.101 ± 0.01	0.935LysAsp: 0.935 ± 0.032	1.115LysGlu: 1.115 ± 0.034	0.408LysPhe: 0.408 ± 0.02	1.157LysGly: 1.157 ± 0.035	0.475LysHis: 0.475 ± 0.022	0.739LysIle: 0.739 ± 0.03	0.448LysLys: 0.448 ± 0.021	1.551LysLeu: 1.551 ± 0.038	0.317LysMet: 0.317 ± 0.016	0.492LysAsn: 0.492 ± 0.022	0.858LysPro: 0.858 ± 0.027	0.658LysGln: 0.658 ± 0.023	1.571LysArg: 1.571 ± 0.037	0.986LysSer: 0.986 ± 0.033	1.273LysThr: 1.273 ± 0.036	1.021LysVal: 1.021 ± 0.035	0.185LysTrp: 0.185 ± 0.012	0.486LysTyr: 0.486 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	10.616LeuAla: 10.616 ± 0.114	0.675LeuCys: 0.675 ± 0.024	5.821LeuAsp: 5.821 ± 0.072	12.429LeuGlu: 12.429 ± 0.117	3.054LeuPhe: 3.054 ± 0.064	7.164LeuGly: 7.164 ± 0.075	1.4LeuHis: 1.4 ± 0.038	3.101LeuIle: 3.101 ± 0.056	1.321LeuLys: 1.321 ± 0.037	8.424LeuLeu: 8.424 ± 0.112	1.176LeuMet: 1.176 ± 0.034	1.588LeuAsn: 1.588 ± 0.041	3.939LeuPro: 3.939 ± 0.069	1.985LeuGln: 1.985 ± 0.041	4.967LeuArg: 4.967 ± 0.06	5.353LeuSer: 5.353 ± 0.065	5.041LeuThr: 5.041 ± 0.07	8.002LeuVal: 8.002 ± 0.106	0.85LeuTrp: 0.85 ± 0.027	2.19LeuTyr: 2.19 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	1.742MetAla: 1.742 ± 0.039	0.12MetCys: 0.12 ± 0.011	1.244MetAsp: 1.244 ± 0.036	1.093MetGlu: 1.093 ± 0.032	0.498MetPhe: 0.498 ± 0.02	1.409MetGly: 1.409 ± 0.032	0.296MetHis: 0.296 ± 0.014	0.796MetIle: 0.796 ± 0.026	0.392MetLys: 0.392 ± 0.019	1.486MetLeu: 1.486 ± 0.036	0.306MetMet: 0.306 ± 0.018	0.561MetAsn: 0.561 ± 0.02	0.768MetPro: 0.768 ± 0.026	0.494MetGln: 0.494 ± 0.021	1.006MetArg: 1.006 ± 0.028	1.421MetSer: 1.421 ± 0.032	1.5MetThr: 1.5 ± 0.035	1.207MetVal: 1.207 ± 0.032	0.16MetTrp: 0.16 ± 0.012	0.395MetTyr: 0.395 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.534AsnAla: 2.534 ± 0.046	0.214AsnCys: 0.214 ± 0.014	1.663AsnAsp: 1.663 ± 0.038	1.904AsnGlu: 1.904 ± 0.041	0.586AsnPhe: 0.586 ± 0.023	2.104AsnGly: 2.104 ± 0.047	0.503AsnHis: 0.503 ± 0.021	0.765AsnIle: 0.765 ± 0.027	0.376AsnLys: 0.376 ± 0.019	1.687AsnLeu: 1.687 ± 0.04	0.396AsnMet: 0.396 ± 0.018	0.459AsnAsn: 0.459 ± 0.021	1.354AsnPro: 1.354 ± 0.034	0.561AsnGln: 0.561 ± 0.023	1.641AsnArg: 1.641 ± 0.039	0.895AsnSer: 0.895 ± 0.027	1.131AsnThr: 1.131 ± 0.038	2.216AsnVal: 2.216 ± 0.046	0.314AsnTrp: 0.314 ± 0.016	0.634AsnTyr: 0.634 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	4.572ProAla: 4.572 ± 0.076	0.23ProCys: 0.23 ± 0.014	4.994ProAsp: 4.994 ± 0.073	4.53ProGlu: 4.53 ± 0.071	1.552ProPhe: 1.552 ± 0.034	3.438ProGly: 3.438 ± 0.049	0.857ProHis: 0.857 ± 0.025	2.207ProIle: 2.207 ± 0.037	0.783ProLys: 0.783 ± 0.024	3.667ProLeu: 3.667 ± 0.063	0.829ProMet: 0.829 ± 0.028	1.136ProAsn: 1.136 ± 0.031	2.065ProPro: 2.065 ± 0.039	0.93ProGln: 0.93 ± 0.029	2.262ProArg: 2.262 ± 0.049	2.604ProSer: 2.604 ± 0.05	3.309ProThr: 3.309 ± 0.054	3.872ProVal: 3.872 ± 0.055	0.516ProTrp: 0.516 ± 0.02	1.06ProTyr: 1.06 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	2.232GlnAla: 2.232 ± 0.049	0.154GlnCys: 0.154 ± 0.009	1.309GlnAsp: 1.309 ± 0.036	1.696GlnGlu: 1.696 ± 0.045	1.042GlnPhe: 1.042 ± 0.026	1.487GlnGly: 1.487 ± 0.039	0.534GlnHis: 0.534 ± 0.022	1.126GlnIle: 1.126 ± 0.036	0.495GlnLys: 0.495 ± 0.021	2.495GlnLeu: 2.495 ± 0.044	0.497GlnMet: 0.497 ± 0.019	0.645GlnAsn: 0.645 ± 0.025	1.136GlnPro: 1.136 ± 0.03	0.986GlnGln: 0.986 ± 0.033	1.93GlnArg: 1.93 ± 0.046	1.538GlnSer: 1.538 ± 0.04	1.538GlnThr: 1.538 ± 0.043	1.651GlnVal: 1.651 ± 0.035	0.323GlnTrp: 0.323 ± 0.016	0.825GlnTyr: 0.825 ± 0.026	0.002GlnXaa: 0.002 ± 0.001
Arg	6.096ArgAla: 6.096 ± 0.092	0.514ArgCys: 0.514 ± 0.024	5.216ArgAsp: 5.216 ± 0.069	6.848ArgGlu: 6.848 ± 0.092	2.276ArgPhe: 2.276 ± 0.051	4.391ArgGly: 4.391 ± 0.064	1.234ArgHis: 1.234 ± 0.032	3.1ArgIle: 3.1 ± 0.054	1.291ArgLys: 1.291 ± 0.031	6.179ArgLeu: 6.179 ± 0.071	1.273ArgMet: 1.273 ± 0.031	1.631ArgAsn: 1.631 ± 0.036	2.584ArgPro: 2.584 ± 0.052	1.687ArgGln: 1.687 ± 0.039	5.179ArgArg: 5.179 ± 0.092	3.962ArgSer: 3.962 ± 0.066	4.318ArgThr: 4.318 ± 0.07	4.922ArgVal: 4.922 ± 0.068	0.779ArgTrp: 0.779 ± 0.027	1.983ArgTyr: 1.983 ± 0.045	0.001ArgXaa: 0.001 ± 0.001
Ser	5.483SerAla: 5.483 ± 0.072	0.344SerCys: 0.344 ± 0.019	4.858SerAsp: 4.858 ± 0.076	4.577SerGlu: 4.577 ± 0.081	1.873SerPhe: 1.873 ± 0.039	4.917SerGly: 4.917 ± 0.065	1.014SerHis: 1.014 ± 0.028	2.759SerIle: 2.759 ± 0.055	1.058SerLys: 1.058 ± 0.03	4.939SerLeu: 4.939 ± 0.069	1.091SerMet: 1.091 ± 0.032	1.377SerAsn: 1.377 ± 0.039	2.526SerPro: 2.526 ± 0.042	1.319SerGln: 1.319 ± 0.034	3.452SerArg: 3.452 ± 0.058	3.633SerSer: 3.633 ± 0.066	3.608SerThr: 3.608 ± 0.055	4.881SerVal: 4.881 ± 0.072	0.608SerTrp: 0.608 ± 0.02	1.392SerTyr: 1.392 ± 0.03	0.001SerXaa: 0.001 ± 0.001
Thr	7.081ThrAla: 7.081 ± 0.079	0.408ThrCys: 0.408 ± 0.018	6.294ThrAsp: 6.294 ± 0.08	4.951ThrGlu: 4.951 ± 0.077	2.009ThrPhe: 2.009 ± 0.045	5.608ThrGly: 5.608 ± 0.071	1.203ThrHis: 1.203 ± 0.032	3.4ThrIle: 3.4 ± 0.054	1.019ThrLys: 1.019 ± 0.029	5.638ThrLeu: 5.638 ± 0.077	1.065ThrMet: 1.065 ± 0.028	1.453ThrAsn: 1.453 ± 0.039	3.031ThrPro: 3.031 ± 0.049	1.372ThrGln: 1.372 ± 0.035	3.305ThrArg: 3.305 ± 0.054	2.919ThrSer: 2.919 ± 0.046	4.201ThrThr: 4.201 ± 0.07	7.105ThrVal: 7.105 ± 0.085	0.605ThrTrp: 0.605 ± 0.021	1.64ThrTyr: 1.64 ± 0.037	0.001ThrXaa: 0.001 ± 0.001
Val	9.761ValAla: 9.761 ± 0.106	0.73ValCys: 0.73 ± 0.028	8.328ValAsp: 8.328 ± 0.099	7.505ValGlu: 7.505 ± 0.083	2.876ValPhe: 2.876 ± 0.053	7.497ValGly: 7.497 ± 0.102	1.686ValHis: 1.686 ± 0.043	3.413ValIle: 3.413 ± 0.061	1.12ValLys: 1.12 ± 0.03	7.951ValLeu: 7.951 ± 0.092	1.276ValMet: 1.276 ± 0.036	1.704ValAsn: 1.704 ± 0.042	4.195ValPro: 4.195 ± 0.061	1.787ValGln: 1.787 ± 0.04	5.275ValArg: 5.275 ± 0.071	4.951ValSer: 4.951 ± 0.065	5.846ValThr: 5.846 ± 0.072	8.677ValVal: 8.677 ± 0.102	0.779ValTrp: 0.779 ± 0.024	2.114ValTyr: 2.114 ± 0.044	0.002ValXaa: 0.002 ± 0.001
Trp	0.884TrpAla: 0.884 ± 0.025	0.104TrpCys: 0.104 ± 0.007	0.79TrpAsp: 0.79 ± 0.032	0.844TrpGlu: 0.844 ± 0.029	0.463TrpPhe: 0.463 ± 0.019	0.809TrpGly: 0.809 ± 0.025	0.244TrpHis: 0.244 ± 0.012	0.564TrpIle: 0.564 ± 0.021	0.275TrpLys: 0.275 ± 0.015	1.293TrpLeu: 1.293 ± 0.038	0.207TrpMet: 0.207 ± 0.013	0.352TrpAsn: 0.352 ± 0.017	0.424TrpPro: 0.424 ± 0.018	0.372TrpGln: 0.372 ± 0.016	0.809TrpArg: 0.809 ± 0.03	0.607TrpSer: 0.607 ± 0.021	0.789TrpThr: 0.789 ± 0.027	0.778TrpVal: 0.778 ± 0.027	0.187TrpTrp: 0.187 ± 0.014	0.355TrpTyr: 0.355 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.832TyrAla: 2.832 ± 0.053	0.276TyrCys: 0.276 ± 0.016	2.767TyrAsp: 2.767 ± 0.048	2.634TyrGlu: 2.634 ± 0.049	0.855TyrPhe: 0.855 ± 0.027	2.373TyrGly: 2.373 ± 0.039	0.658TyrHis: 0.658 ± 0.026	0.723TyrIle: 0.723 ± 0.027	0.396TyrLys: 0.396 ± 0.02	2.446TyrLeu: 2.446 ± 0.046	0.384TyrMet: 0.384 ± 0.018	0.551TyrAsn: 0.551 ± 0.019	1.199TyrPro: 1.199 ± 0.032	0.691TyrGln: 0.691 ± 0.026	1.921TyrArg: 1.921 ± 0.033	1.16TyrSer: 1.16 ± 0.032	1.432TyrThr: 1.432 ± 0.033	2.371TyrVal: 2.371 ± 0.042	0.351TyrTrp: 0.351 ± 0.015	0.85TyrTyr: 0.85 ± 0.026	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.002XaaTrp: 0.002 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4400 proteins (1253988 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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