Roseburia sp. 499

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; unclassified Roseburia

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9JZD8|A0A1Q9JZD8_9FIRM GntR family transcriptional regulator OS=Roseburia sp. 499 OX=1261634 GN=BIV20_04880 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.23RR3 pKa = 11.84KK4 pKa = 9.3LVSLMLVAAMAASMVVGCGNNSNDD28 pKa = 4.42KK29 pKa = 10.98GSNDD33 pKa = 3.21KK34 pKa = 11.34GKK36 pKa = 8.37TEE38 pKa = 3.89EE39 pKa = 4.29AGGEE43 pKa = 4.03KK44 pKa = 10.48DD45 pKa = 3.6EE46 pKa = 5.0SGSTASGDD54 pKa = 3.24GRR56 pKa = 11.84VYY58 pKa = 11.19LLNFKK63 pKa = 10.65PEE65 pKa = 4.02TDD67 pKa = 4.09QAWQDD72 pKa = 3.57LADD75 pKa = 3.84VYY77 pKa = 11.05TDD79 pKa = 3.39EE80 pKa = 4.89TGVEE84 pKa = 4.43VNVLTAADD92 pKa = 4.09GQYY95 pKa = 9.12STTMQSEE102 pKa = 4.35MAKK105 pKa = 10.63DD106 pKa = 3.41EE107 pKa = 4.5APTIFNIGNTTAAQTWNDD125 pKa = 3.39YY126 pKa = 9.18TLDD129 pKa = 3.91LKK131 pKa = 11.18DD132 pKa = 3.62SEE134 pKa = 4.94LYY136 pKa = 10.46KK137 pKa = 10.9HH138 pKa = 6.18LTDD141 pKa = 3.83KK142 pKa = 11.03SLSITYY148 pKa = 9.37DD149 pKa = 3.23GKK151 pKa = 10.26IAAVANCYY159 pKa = 8.88EE160 pKa = 4.63CYY162 pKa = 10.64GIIYY166 pKa = 9.51NKK168 pKa = 10.3KK169 pKa = 9.51ILNDD173 pKa = 3.36YY174 pKa = 7.56CTLDD178 pKa = 3.54GAVIASPDD186 pKa = 3.94EE187 pKa = 3.92ITSFDD192 pKa = 3.54TLKK195 pKa = 11.08AVAEE199 pKa = 4.77DD200 pKa = 3.05INARR204 pKa = 11.84VDD206 pKa = 3.91EE207 pKa = 4.69INDD210 pKa = 3.27EE211 pKa = 4.38FGYY214 pKa = 10.42EE215 pKa = 3.89LQGAFASAGLDD226 pKa = 3.32SGSSWRR232 pKa = 11.84FSGHH236 pKa = 6.45LANMPLYY243 pKa = 11.0YY244 pKa = 10.18EE245 pKa = 4.8FKK247 pKa = 10.8DD248 pKa = 4.85DD249 pKa = 5.15GCDD252 pKa = 3.98LINGEE257 pKa = 4.14ATIDD261 pKa = 3.57GTYY264 pKa = 10.34LDD266 pKa = 3.82QYY268 pKa = 9.78KK269 pKa = 10.42AVWDD273 pKa = 4.42LYY275 pKa = 10.95VNTSGADD282 pKa = 3.45PKK284 pKa = 10.21TLNSGALNAEE294 pKa = 4.44SEE296 pKa = 4.48FGMEE300 pKa = 4.02EE301 pKa = 3.67AVFYY305 pKa = 11.31QNGDD309 pKa = 3.31WEE311 pKa = 4.58FSPLTSDD318 pKa = 3.23EE319 pKa = 3.96NGYY322 pKa = 10.35LVTADD327 pKa = 5.35DD328 pKa = 4.01IGMMPIYY335 pKa = 10.46FGVDD339 pKa = 3.35DD340 pKa = 4.94EE341 pKa = 4.57NQGLCVGTEE350 pKa = 4.14NYY352 pKa = 8.28WAVNSQASQEE362 pKa = 4.92DD363 pKa = 3.4IDD365 pKa = 3.84ATLAFLEE372 pKa = 4.41WVITSDD378 pKa = 3.41EE379 pKa = 4.07GRR381 pKa = 11.84DD382 pKa = 3.63AITNTMGLSAPYY394 pKa = 8.83DD395 pKa = 3.92TFTGDD400 pKa = 3.52YY401 pKa = 9.52EE402 pKa = 4.37SANAFVQDD410 pKa = 3.87SNALMSAGKK419 pKa = 8.67TSVAWSFNATPNVDD433 pKa = 3.0DD434 pKa = 3.57WRR436 pKa = 11.84ADD438 pKa = 3.45VVSALTAYY446 pKa = 9.73TDD448 pKa = 4.83GSGDD452 pKa = 3.09WDD454 pKa = 3.61AVKK457 pKa = 9.09TAFVDD462 pKa = 4.35GWANQWTLAHH472 pKa = 7.02EE473 pKa = 5.36DD474 pKa = 3.71DD475 pKa = 4.31AQQ477 pKa = 4.77

Molecular weight: 51.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9K4F6|A0A1Q9K4F6_9FIRM Tyrosine--tRNA ligase OS=Roseburia sp. 499 OX=1261634 GN=tyrS PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3105

0

3105

974933

37

2567

314.0

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.795 ± 0.04

1.435 ± 0.018

5.371 ± 0.035

8.715 ± 0.06

4.05 ± 0.028

6.692 ± 0.037

1.617 ± 0.018

7.745 ± 0.045

7.381 ± 0.043

8.647 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.265 ± 0.022

4.725 ± 0.034

2.921 ± 0.021

3.541 ± 0.031

3.969 ± 0.028

5.674 ± 0.035

5.275 ± 0.041

6.967 ± 0.038

0.871 ± 0.015

4.343 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski